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1.8.1.9: thioredoxin-disulfide reductase

This is an abbreviated version!
For detailed information about thioredoxin-disulfide reductase, go to the full flat file.

Word Map on EC 1.8.1.9

Reaction

thioredoxin
+
NADP+
=
thioredoxin disulfide
 +
NADPH
 +
H+

Synonyms

all0737, ApTR, At2g41680, AtNTRA, AtNTRB, bacillithiol disulfide reductase, bacillithiol-disulfide reductase, BBOV_I002190, Bdr, BSSB reductase, CeTR2, DmTR, DmTrxR, DmTrxR-1, EC 1.6.4.5, EhTRXR, ferredoxin:thioredoxin reductase, FTR, general stress protein 35, GSP35, HCOI_01258400, hemolysate thioredoxin reductase, hemolysate TR, hTrxR, HvNTR1, HvNTR2, L-TR, L-TrxR, More, MtNTRC, mTR3, multifunctional thioredoxin-glutathione reductase, NAD(P)H:paraquat oxidoreductase, NADP-dependent thioredoxin reductase, NADP-linked thioredoxin reductase, NADP-thioredoxin reductase, NADP-thioredoxin reductase C, NADPH thioredoxin reductase, NADPH thioredoxin reductase C, NADPH-dependent thioredoxin reductase, NADPH-dependent thioredoxin reductase 2, NADPH-dependent thioredoxin reductase C, NADPH-dependent thioredoxin reductase I, NADPH-dependent thioredoxin reductase-1, NADPH-dependent TRX reductase, NADPH-thioredoxin reductase, NADPH-thioredoxin reductase C, NADPH-Trx reductase, NADPH2:oxidized thioredoxin oxidoreductase, NAPDH-dependent BSSB reductase, NTR, Ntr1, NTR2, Ntr3, NTRA, NTRAB, NtrB, NTRC, PH0178, PH1426, PhRP, PhTrxR, protein-disulfide oxidoreductase, reductase, thioredoxin, SEP1, taTrxR, TGR, thioredoxin glutathione reductase, thioredoxin h reductase, thioredoxin reductase, thioredoxin reductase (NADPH), thioredoxin reductase 1, thioredoxin reductase 2, thioredoxin reductase-1, thioredoxin reductase1, TON_1603, TR, TR1, TR2, TR3, TRase, TrR, Trr1, Trr1p, TRR2, Trx1, Trx3, TrxB, TrxB1, TrxB2, TrxR, TrxR-1(cyto), TrxR-1(mito), TrxR1, TrxR2, TrxR3, TrxRB3, TRXRD, TrxRh1, TrxRh2, TrxT, Txnrd1, TXNRD1-v3, Txnrd2, TXNRD3, TxrR-1, YpdA

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.9 thioredoxin-disulfide reductase

IC50 Value

IC50 Value on EC 1.8.1.9 - thioredoxin-disulfide reductase

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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0018
(1E,4E)-1,5-bis(3,4-dihydroxyphenyl)penta-1,4-dien-3-one
Sus scrofa
-
pH 7.4, 25°C
0.0137
(1E,4E)-1,5-bis(3,5-di-tert-butyl-4-hydroxyphenyl)penta-1,4-dien-3-one
Sus scrofa
-
pH 7.4, 25°C
0.003
(1E,4E)-1,5-bis(3-bromo-4-hydroxy-5-methoxyphenyl)penta-1,4-dien-3-one
Sus scrofa
-
pH 7.4, 25°C
0.0017
(1E,4E)-1,5-bis(4-hydroxy-3,5-dimethoxyphenyl)penta-1,4-dien-3-one
Sus scrofa
-
pH 7.4, 25°C
0.0063
(1E,4E)-1,5-bis(4-hydroxyphenyl)penta-1,4-dien-3-one
Sus scrofa
-
pH 7.4, 25°C
0.0383
(1E,4Z,6E)-1,7-di-2-furyl-5-hydroxyhepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.05
(1E,4Z,6E)-1-(2-bromophenyl)-5-hydroxy-7-(4-hydroxyphenyl)hepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.0622
(1E,4Z,6E)-1-[4-(dimethylamino)phenyl]-5-hydroxy-7-[5-(hydroxymethyl)-2-furyl]hepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.0003
(1E,4Z,6E)-5-hydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.0016
(1E,4Z,6E)-5-hydroxy-1,7-bis(5-methyl-2-furyl)hepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.0005
(1E,4Z,6E)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-7-(5-methyl-2-furyl)hepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.001
(1E,4Z,6E)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-7-[5-(hydroxymethyl)-2-furyl]hepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.02
(1E,4Z,6E)-5-hydroxy-1-(4-hydroxyphenyl)-7-(2-thienyl)hepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.02
(1E,4Z,6E)-5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-(4-hydroxyphenyl)hepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.057
(1E,4Z,6E)-5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenylhepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.0138
(1E,4Z,6E)-5-hydroxy-7-(4-hydroxyphenyl)-1-(3,4,5-trimethoxyphenyl)hepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.0233
(1E,4Z,6E)-5-hydroxy-7-(4-hydroxyphenyl)-1-phenylhepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.002
(1E,4Z,6E)-5-hydroxy-7-[5-(hydroxymethyl)-2-furyl]-1-(3,4,5-trimethoxyphenyl)hepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.0008
(1E,4Z,6E)-5-hydroxy-7-[5-(hydroxymethyl)-2-furyl]-1-(4-methoxyphenyl)hepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.0013
(1E,4Z,6E)-5-hydroxy-7-[5-(hydroxymethyl)-2-furyl]-1-phenylhepta-1,4,6-trien-3-one
Homo sapiens
-
pH 7.4, 37°C
0.0081
(2E,5E)-2,5-bis(3,4-dihydroxybenzylidene)cyclopentanone
Sus scrofa
-
pH 7.4, 25°C
0.0042
(2E,5E)-2,5-bis(3-bromo-4-hydroxy-5-methoxybenzylidene)cyclopentanone
Sus scrofa
-
pH 7.4, 25°C
0.0033
(2E,5E)-2,5-bis(4-hydroxybenzylidene)cyclopentanone
Sus scrofa
-
pH 7.4, 25°C
0.0407
(2E,5E)-2,5-bis[(3,5-di-tert-butyl-4-hydroxyphenyl)methylidene]cyclopentanone
Sus scrofa
-
pH 7.4, 25°C
0.00052
(2E,5E)-2,5-bis[(4-hydroxy-3,5-dimethoxyphenyl)methylidene]cyclopentanone
Sus scrofa
-
pH 7.4, 25°C
0.0048
(2E,6E)-2,6-bis(3,4-dihydroxybenzylidene)cyclohexanone
Sus scrofa
-
pH 7.4, 25°C
0.0089
(2E,6E)-2,6-bis(3-bromo-4-hydroxy-5-methoxybenzylidene)cyclohexanone
Sus scrofa
-
pH 7.4, 25°C
0.0241
(2E,6E)-2,6-bis(4-hydroxybenzylidene)cyclohexanone
Sus scrofa
-
pH 7.4, 25°C
0.0453
(2E,6E)-2,6-bis[(3,4-dimethoxyphenyl)methylidene]cyclohexanone
Sus scrofa
-
pH 7.4, 25°C
0.0064
(2E,6E)-2,6-bis[(4-hydroxy-3,5-dimethoxyphenyl)methylidene]cyclohexanone
Sus scrofa
-
pH 7.4, 25°C
0.0051
(2E,6E)-2-(4-hydroxybenzylidene)-6-(4-hydroxy-3-methoxybenzylidene)cyclohexanone
Homo sapiens
-
pH 7.4, 37°C
0.0249
(2E,6E)-2-[(4-hydroxyphenyl)methylidene]-6-[(3,4,5-trimethoxyphenyl)methylidene]cyclohexanone
Homo sapiens
-
pH 7.4, 37°C
0.0462
(3E,5E)-3,5-bis(3,4-dihydroxybenzylidene)piperidin-4-one
Sus scrofa
-
pH 7.4, 25°C
0.0046
(3E,5E)-3,5-bis(3-bromo-4-hydroxy-5-methoxybenzylidene)piperidin-4-one
Sus scrofa
-
pH 7.4, 25°C
0.071
(3E,5E)-3,5-bis(4-hydroxybenzylidene)-4-oxopiperidinium
Sus scrofa
-
pH 7.4, 25°C
0.0306
(3E,5E)-3,5-bis[(3,4-dimethoxyphenyl)methylidene]piperidin-4-one
Sus scrofa
-
pH 7.4, 25°C
0.00086
(3E,5E)-3,5-bis[(4-hydroxy-3,5-dimethoxyphenyl)methylidene]piperidin-4-one
Sus scrofa
-
pH 7.4, 25°C
0.0184
(3E,5E)-3-[(2,5-di-tert-butyl-4-hydroxyphenyl)methylidene]-5-[(3,5-di-tert-butyl-4-hydroxyphenyl)methylidene]piperidin-4-one
Sus scrofa
-
pH 7.4, 25°C
0.00007442
(4-ammoniothiophenolato)(2,2':6',2''-terpyridine)platinum(II) chloride
Homo sapiens
in 50 mM Tris-HCl, pH 8.0, at 37°C
-
0.00007002
(4-ammoniothiophenolato)(4'-toyl-2,2':6',2''-terpyridine)platinum(II) nitrate
Homo sapiens
in 50 mM Tris-HCl, pH 8.0, at 37°C
-
0.00006977
(4-hydroxylthiophenolato)(2,2':6',2''-terpyridine)platinum(II) chloride
Homo sapiens
in 50 mM Tris-HCl, pH 8.0, at 37°C
-
0.00005826
(4-hydroxylthiophenolato)(4'-toyl-2,2':6',2''-terpyridine)platinum(II) chloride
Homo sapiens
in 50 mM Tris-HCl, pH 8.0, at 37°C
-
0.00000096 - 0.00000406
(4-methylpyrimidine-2-thiolato-kappaS)(1,3,5-triaza-7-phosphatricyclo[3.3.1.13,7]decane-kappaP)gold
0.00000155 - 0.00000586
(4-methylpyrimidine-2-thiolato-kappaS)[1,1'-(1,3,7-triaza-5-phosphabicyclo[3.3.1]nonane-3,7-diyl-kappaP)diethanone]gold
0.00007426
(N-acetyl-4-aminothiophenolato)(2,2':6',2''-terpyridine)platinum(II) chloride
Homo sapiens
in 50 mM Tris-HCl, pH 8.0, at 37°C
-
0.00007786
(N-acetyl-4-aminothiophenolato)(4'-toyl-2,2':6',2''-terpyridine)platinum(II) nitrate
Homo sapiens
in 50 mM Tris-HCl, pH 8.0, at 37°C
-
0.00000161 - 0.00000412
(pyridine-2-thiolato-kappaS)(1,3,5-triaza-7-phosphatricyclo[3.3.1.13,7]decane-kappaP)gold
0.00000154 - 0.0000062
(pyridine-2-thiolato-kappaS)[1,1'-(1,3,7-triaza-5-phosphabicyclo[3.3.1]nonane-3,7-diyl-kappaP)diethanone]gold
0.00000192 - 0.00000673
(pyrimidine-2-thiolato-kappaS)(1,3,5-triaza-7-phosphatricyclo[3.3.1.13,7]decane-kappaP)gold
0.0005 - 0.004
1,1'-sulfanediylbis(2,4-dinitrobenzene)
0.02 - 0.15
1,1'-sulfanediylbis[2-nitro-4-(trifluoromethyl)benzene]
0.03 - 0.2
1,3-dinitro-5-(trifluoromethyl)benzene
0.2
1,4-dihydroxyanthroquinone
0.2
1,8-dihydroxyanthroquinone
Rattus norvegicus
IC50 above 0.2 mM, isoform TrxR1, at 25°C in 0.2 M Na, K-phosphate buffer (pH 7.4)
0.00225
1-chloro-2,4-dinitrobenzene
Haemonchus contortus
-
pH 7.8, 37°C
0.00036
15-deoxy-D-12,14-PGJ2
Rattus norvegicus
-
0.06 mM, IC50: 0.00036 mM
0.0086
2-aminothiazolium [trans-tetrachlorobis(2-aminothiazole)ruthenate(III)]
Rattus norvegicus
-
0.017 - 0.0994
2-benzoyloxycinnamaldehyde
0.0636
2-benzyloxycinnamaldehyde
Rattus norvegicus
-
1 h incubation with recombinant TrxR, in TE buffer, at 25°C
0.008 - 0.1
2-chloro-1,3-dinitro-5-(trifluoromethyl)benzene
0.0253
2-hydroxycinnamaldehyde
Rattus norvegicus
-
1 h incubation with recombinant TrxR, in TE buffer, at 25°C
0.000146
2-hydroxymethyl-5-methoxy-1-methyl-3-[(2,4,6-trifluorophenoxy)methyl]indole-4,7-dione
Rattus norvegicus
-
in 100 mM potassium phosphate buffer, pH 7.4, at 22°C
0.1
2-pentoxycinnamaldehyde
Rattus norvegicus
-
1 h incubation with recombinant TrxR, in TE buffer, at 25°C
0.02
3,4-estronequinone
Rattus norvegicus
-
0.032 mM, IC50: 0.02 mM
0.0083
3-(4-[[2-(1-hydroxy-4-oxocyclohexa-2,5-dien-1-yl)-1H-indol-1-yl]sulfonyl]phenyl)propanoic acid
Rattus norvegicus
-
pH 7.5, determined after 60 min incubation of recombinant rat TrxR with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, irreversible
0.0098
3-(4-[[6-fluoro-2-(1-hydroxy-4-oxocyclohexa-2,5-dien-1-yl)-1H-indol-1-yl]sulfonyl]phenyl)propanoic acid
Rattus norvegicus
-
pH 7.5, determined after 60 min incubation of recombinant rat TrxR with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, irreversible
0.01 - 0.08
4,5-dinitro-1,3-benzodioxole
0.002 - 0.01
4,6-dinitro-2,1,3-benzothiadiazole
0.0065
4-(1,3-benzothiazol-2-yl)-4-hydroxycyclohexa-2,5-dien-1-one
Rattus norvegicus
-
pH 7.5, determined after 60 min incubation of recombinant rat TrxR with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, irreversible
0.0761
4-(1,3-benzoxazol-2-yl)-4-hydroxycyclohexa-2,5-dien-1-one
Rattus norvegicus
-
pH 7.5, determined after 60 min incubation of recombinant rat TrxR with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, irreversible
0.1043
4-(1-benzothien-2-yl)-4-hydroxycyclohexa-2,5-dien-1-one
Rattus norvegicus
-
pH 7.5, determined after 60 min incubation of recombinant rat TrxR with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, irreversible
0.0063
4-(5-fluoro-1,3-benzothiazol-2-yl)-4-hydroxycyclohexa-2,5-dien-1-one
Rattus norvegicus
-
pH 7.5, determined after 60 min incubation of recombinant rat TrxR with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, irreversible
0.0038
4-hydroxy-2-nonenal
Rattus norvegicus
-
0.005-0.025 mM, IC50: 0.0038 mM, irreversible inhibition
0.0029
4-hydroxy-4-[1-(phenylsulfonyl)-1H-indol-2-yl]cyclohexa-2,5-dien-1-one
Rattus norvegicus
-
pH 7.5, determined after 60 min incubation of recombinant rat TrxR with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, irreversible
0.012
4-hydroxynonenal
Rattus norvegicus
-
0.06 mM, IC50: 0.012 mM
0.002 - 0.05
4-nitro-2,1,3-benzothiadiazole
0.0027
4-[6-fluoro-1-(phenylsulfonyl)-1H-indol-2-yl]-4-hydroxycyclohexa-2,5-dien-1-one
Rattus norvegicus
-
pH 7.5, determined after 60 min incubation of recombinant rat TrxR with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, irreversible
0.007 - 0.0844
5-fluoro-2-hydroxycinnamaldehyde
0.000082
5-methoxy-1,2-dimethyl-3-[1-oxo-2-(2,4,6-trifluorophenyl)ethyl]indole-4,7-dione
Rattus norvegicus
-
in 100 mM potassium phosphate buffer, pH 7.4, at 22°C
0.2
5-nitro-1,3-benzodioxole
Homo sapiens
-
IC50: 0.2 mM
0.01 - 0.09
5-nitro-2,1,3-benzothiadiazole
0.002 - 0.14
6,7-dinitroquinoxaline
0.04 - 0.2
6-nitroquinoxaline
0.019
Ag+
Meleagris gallopavo
-
pH 7.5, 50°C
0.0205
allyl isothiocyanate
Homo sapiens
-
25°C, 30 min preincubation in assay with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate
0.18 - 0.2
aloe emodin
0.2
aloin A
0.2
anthrone
0.00000074 - 0.00000245
auranofin
0.00012
aurothioglucose
Fasciola hepatica
-
IC50: 120 nM
0.0033
Benzyl isothiocyanate
Homo sapiens
-
25°C, 30 min preincubation in assay with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate
0.529
Cd2+
Meleagris gallopavo
-
pH 7.5, 50°C
0.004
chaetocin
Homo sapiens
-
using 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, in 100 mM potassium phosphate (pH 7.0), at 22°C
0.00023
chloro[N(4)-ortho-chlorophenyl-2-acetylpyridinethiosemicarbazonato]gold(III)dichloroaurate(I)
Rattus norvegicus
-
pH 7.0, 37°C
0.181 - 0.194
chrysophanol
0.0025 - 1.46
Cu2+
0.0036 - 0.0382
curcumin
0.38
diallyl disulfide
Homo sapiens
-
25°C, 30 min preincubation in assay with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate
0.00016
dichloro[N(4)-ortho-chlorophenyl-2-acetylpyridinethiosemicarbazonato]antimony(III)
Rattus norvegicus
-
pH 7.0, 37°C
0.001
diphenylene iodonium
Mus musculus
-
IC50: 0.001 mM
0.61
Fe3+
Meleagris gallopavo
-
pH 7.5, 50°C
0.2
frangulin A
0.049 - 0.198
hypericin
0.513
leukotriene A4 methyl ester
Rattus norvegicus
-
0.06 mM, IC50: 0.513 mM
0.0000171 - 0.000158
methylmercury
0.62
myricetin
Rattus norvegicus
-
0.05 mM, strong inhibitory effect, IC50: 0.62 mM
0.00035
palmarumycin CP1
Homo sapiens
-
0.001 mM, the naphthoquinone spiroketal fungal metabolite palmarumycin CP1 is a potent inhibitor of thioredoxin reductase-1, IC50: 0.00035 mM
0.075
phenethyl isothiocyanate
Homo sapiens
-
25°C, 30 min preincubation in assay with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate
0.132 - 0.185
physcion
0.068
Prostaglandin A2
Rattus norvegicus
-
0.06 mM, IC50: 0.068 mM
0.0046 - 0.0079
pseudohypericin
0.0032
PX-911
Homo sapiens
-
0.001 mM, a water-soluble prodrug of a palmarumycin CP1 analogue, IC50: 0.0032 mM
0.00028
PX-916
Homo sapiens
-
0.001 mM, a water-soluble prodrug of a palmarumycin CP1 analogue, potent inhibitor of purified human thioredoxin reductase-1, IC50: 0.00028 mM
0.00027
PX-960
Homo sapiens
-
0.001 mM, a water-soluble prodrug of a palmarumycin CP1 analogue, IC50: 0.00027 mM
0.97
quercetin
Rattus norvegicus
-
0.05 mM, strong inhibitory effect, IC50: 0.97 mM
0.084 - 0.123
Rhein
0.2
sennoside A
0.2
sennoside B
0.2
skyrin glucoside
0.04
sulforaphane
Homo sapiens
-
25°C, 30 min preincubation in assay with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate
0.06
theaflavin
Bos taurus
IC50 above 0.06 mM, in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5
0.0191
theaflavin-3'-monogallate
Bos taurus
in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5
0.0216
theaflavin-3,3'-digallate
Bos taurus
in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5
0.018
theaflavin-3-monogallate
Bos taurus
in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5
0.0015 - 0.01
trans,trans-curcumin
0.0713
trans-cinnamaldehyde
Rattus norvegicus
-
1 h incubation with recombinant TrxR, in TE buffer, at 25°C
0.0238
trans-[bis(2-amino-5-methylthiazole)tetrachlororuthenate(III)]
Rattus norvegicus
-
0.00000167 - 0.00000682
trisodium (4,5-dihydro-1,3-thiazole-2-thiolato-kappaS2)[3,3',3''-(phosphanetriyl-kappaP)tribenzenesulfonato(3-)]aurate(3-)
0.00000062 - 0.00000695
trisodium [3,3',3''-(phosphanetriyl-kappaP)tribenzenesulfonato(3-)](pyrimidine-2-thiolato-kappaS)aurate(3-)
0.0034 - 3.4
Zn2+
additional information
additional information
Homo sapiens
black tea extract and theaflavins (mixture of theaflavin, theaflavin-3-monogallate, theaflavin-3'-monogallate and theaflavin-3,3'-digallate) inhibit the purified TrxR1 with IC50 44 mg/ml and 21 mg/ml, respectively
-