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0.032 - 11.9
5,5'-dithio-bis(2-nitrobenzoic acid)
0.0186 - 172.4
5,5'-dithiobis(2-nitrobenzoic acid)
0.00227 - 0.02374
5-hydroxy-1,4-naphthoquinone
0.00048 - 0.0543
Arabidopsis thaliana thioredoxin 3
-
0.0042
Babesia bovis thioredoxin
pH 8.0, 30°C
-
0.089
benzyl viologen
-
at pH 9.5 and 80°C
0.0046
chaetocin
-
in 100 mM potassium phosphate (pH 7.0), at 22°C
0.0161
chetomin
-
in 100 mM potassium phosphate (pH 7.0), at 22°C
1.25
dithionitrobenzene
-
pH and temperature not specified in the publication
0.0025
ebsulfur
-
pH 7.5, temperature not specified in the publication
0.0275
ebsulfur disulfide
-
pH 7.5, temperature not specified in the publication
0.0036
Entamoeba histolytica thioredoxin disulfide 41
-
pH 7.0, 25°C
-
0.007 - 0.149
Escherichia coli thioredoxin
-
0.0046
Escherichia coli thioredoxin disulfide
-
pH 7.0, 25°C
-
0.0169
gliotoxin
-
in 100 mM potassium phosphate (pH 7.0), at 22°C
0.0333
glutaredoxin 4
-
-
-
2.5
H2O2
-
wild-type enzyme, Km-value can be reduced by addition of selenocysteine
0.00123
Hordeum vulgare thioredoxin 1
wild-type, pH 7.5, temperature not specified in the publication
-
0.00068 - 0.06
Hordeum vulgare thioredoxin 2
-
0.00041 - 0.0016
Hordeum vulgare thioredoxin 2 mutant E86A
-
0.0014
Hordeum vulgare thioredoxin 2 mutant G57P
wild-type, pH 7.5, temperature not specified in the publication
-
0.0155
Hordeum vulgare thioredoxin 2 mutant I51G
wild-type, pH 7.5, temperature not specified in the publication
-
0.00112 - 0.00118
Hordeum vulgare thioredoxin disulfide h1
-
0.0009 - 0.00179
Hordeum vulgare thioredoxin disulfide h2
-
0.0033
human thioredoxin
-
wild-type enzyme
6.6
hydrogen peroxide
semisynthetic enzyme with 91% content of selenium
0.0027
lipoic acid
-
isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C
0.134
methaneseleninic acid
-
at pH 6.1 and 37°C
0.018
methylseleninate
-
-
0.035
protein disulfide-isomerase
-
-
-
0.0025 - 0.0033
reductase
-
0.00031 - 67.6
thioredoxin
0.0013
thioredoxin 1
-
wild type enzyme, pH and temperature not specified in the publication
0.0006 - 0.0009
thioredoxin 2
-
0.0011
thioredoxin 3
-
wild type enzyme, pH and temperature not specified in the publication
-
0.0023 - 0.0036
thioredoxin 41
-
0.0028 - 0.0029
thioredoxin 8
-
0.00047
thioredoxin C-2
-
-
-
0.00099 - 0.173
thioredoxin disulfide
0.0036
thioredoxin disulfide 41
-
pH 7, 30°C
-
0.0028
thioredoxin disulfide 8
-
pH 7, 30°C
-
0.0076
thioredoxin disulfide h
-
-
0.0067
thioredoxin K36R
-
substrate mutant, pH 8.0
-
0.007 - 0.019
thioredoxin-1 disulfide
-
0.0044
thioredoxin-II
-
-
-
0.125
thioredoxin-R
-
substrate mutant, thioredoxin-CAC, pH 8.0
-
additional information
additional information
-
0.032
5,5'-dithio-bis(2-nitrobenzoic acid)
-
pH 7.5, 50°C
0.238
5,5'-dithio-bis(2-nitrobenzoic acid)
pH 8.0, 30°C
0.28
5,5'-dithio-bis(2-nitrobenzoic acid)
-
pH 7.8, 37°C
2.4
5,5'-dithio-bis(2-nitrobenzoic acid)
pH 7.2, temperature not specified in the publication
2.5
5,5'-dithio-bis(2-nitrobenzoic acid)
pH 7.2, temperature not specified in the publication
11.9
5,5'-dithio-bis(2-nitrobenzoic acid)
truncated mutant, pH 7.2, temperature not specified in the publication
0.0186
5,5'-dithiobis(2-nitrobenzoic acid)
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
0.049
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
0.088
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme
0.09
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, 10 mM potassium phosphate buffer (pH 7.0) containing 10 mM EDTA, at 25°C
0.0906
5,5'-dithiobis(2-nitrobenzoic acid)
mutant enzyme Y116T, in TE buffer, at 20°C
0.094
5,5'-dithiobis(2-nitrobenzoic acid)
wild type enzyme, in TE buffer, at 20°C
0.0971
5,5'-dithiobis(2-nitrobenzoic acid)
mutant enzyme Y116I, in TE buffer, at 20°C
0.105
5,5'-dithiobis(2-nitrobenzoic acid)
-
isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C
0.114
5,5'-dithiobis(2-nitrobenzoic acid)
-
full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C
0.139
5,5'-dithiobis(2-nitrobenzoic acid)
purified, recombinant, tetrameric enzyme
0.17
5,5'-dithiobis(2-nitrobenzoic acid)
full-length Drosophila enzyme with C-terminal sequence SCCS
0.2156
5,5'-dithiobis(2-nitrobenzoic acid)
purified, recombinant, dimeric enzyme
0.22
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
0.39
5,5'-dithiobis(2-nitrobenzoic acid)
-
isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C
0.41
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
0.42
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme
0.45
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
0.463
5,5'-dithiobis(2-nitrobenzoic acid)
-
in 50 mM potassium phosphate buffer (pH 7.0), at 22°C
0.47
5,5'-dithiobis(2-nitrobenzoic acid)
full-length mouse enzyme with C-terminal sequence GCUG
0.53
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
0.7
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH 7.4
0.7
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant U498C
0.71
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C
0.75
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH 7, 25°C
0.83
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
0.92
5,5'-dithiobis(2-nitrobenzoic acid)
truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid
1.1
5,5'-dithiobis(2-nitrobenzoic acid)
-
TrxR-16 mutant K29R/H108Y/A119N/V478E
1.1
5,5'-dithiobis(2-nitrobenzoic acid)
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
1.4
5,5'-dithiobis(2-nitrobenzoic acid)
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
1.7
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH 7.0, 25°C
2.4
5,5'-dithiobis(2-nitrobenzoic acid)
-
TrxR-16, TrxR lacking the last 16 C-terminal amino acids
2.72
5,5'-dithiobis(2-nitrobenzoic acid)
truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid
3.3
5,5'-dithiobis(2-nitrobenzoic acid)
-
TrxR-16 mutant K29R
4.1
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
4.5
5,5'-dithiobis(2-nitrobenzoic acid)
-
TrxR-16 mutant K29R/H108Y
172.4
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
0.00227
5-hydroxy-1,4-naphthoquinone
wild type enzyme, in TE buffer, at 20°C
0.00698
5-hydroxy-1,4-naphthoquinone
mutant enzyme Y116T, in TE buffer, at 20°C
0.02374
5-hydroxy-1,4-naphthoquinone
mutant enzyme Y116I, in TE buffer, at 20°C
0.00048
Arabidopsis thaliana thioredoxin 3
pH 7.5, temperature not specified in the publication
-
0.00052
Arabidopsis thaliana thioredoxin 3
mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication
-
0.00067
Arabidopsis thaliana thioredoxin 3
mutant G225R/G226D, pH 7.5, temperature not specified in the publication
-
0.00073
Arabidopsis thaliana thioredoxin 3
mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication
-
0.0008
Arabidopsis thaliana thioredoxin 3
wild-type, pH 7.5, temperature not specified in the publication
-
0.0024
Arabidopsis thaliana thioredoxin 3
mutant W42A, pH 7.5, temperature not specified in the publication
-
0.0042
Arabidopsis thaliana thioredoxin 3
mutant N45A/D46A, pH 7.5, temperature not specified in the publication
-
0.0141
Arabidopsis thaliana thioredoxin 3
mutant M43A, pH 7.5, temperature not specified in the publication
-
0.0305
Arabidopsis thaliana thioredoxin 3
mutant W42A/M43A, pH 7.5, temperature not specified in the publication
-
0.0543
Arabidopsis thaliana thioredoxin 3
mutant Delta42-47, pH 7.5, temperature not specified in the publication
-
0.05
DTNB
-
mutant enzyme H509Q
0.147
DTNB
-
mutant enzyme H509A
0.212
DTNB
-
wild-type enzyme
0.38
DTNB
-
cytosolic TrxR1 isoform
0.41
DTNB
-
mitochondrial TrxR1 isoform
0.007
Escherichia coli thioredoxin
pH 8.0, 30°C
-
0.149
Escherichia coli thioredoxin
wild-type, pH 7.5, temperature not specified in the publication
-
0.00068
Hordeum vulgare thioredoxin 2
mutant N139A, pH 7.5, temperature not specified in the publication
-
0.00105
Hordeum vulgare thioredoxin 2
mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication
-
0.00117
Hordeum vulgare thioredoxin 2
wild-type, pH 7.5, temperature not specified in the publication
-
0.00128
Hordeum vulgare thioredoxin 2
mutant G225R/G226D, pH 7.5, temperature not specified in the publication
-
0.00146
Hordeum vulgare thioredoxin 2
mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication
-
0.0022
Hordeum vulgare thioredoxin 2
mutant N45A/D46A, pH 7.5, temperature not specified in the publication
-
0.00343
Hordeum vulgare thioredoxin 2
mutant R140A, pH 7.5, temperature not specified in the publication
-
0.016
Hordeum vulgare thioredoxin 2
mutant M43A, pH 7.5, temperature not specified in the publication
-
0.0457
Hordeum vulgare thioredoxin 2
mutant W42A, pH 7.5, temperature not specified in the publication
-
0.06
Hordeum vulgare thioredoxin 2
pH 7.5, temperature not specified in the publication
-
0.00041
Hordeum vulgare thioredoxin 2 mutant E86A
mutant N139A, pH 7.5, temperature not specified in the publication
-
0.00057
Hordeum vulgare thioredoxin 2 mutant E86A
wild-type, pH 7.5, temperature not specified in the publication
-
0.0016
Hordeum vulgare thioredoxin 2 mutant E86A
mutant R140A, pH 7.5, temperature not specified in the publication
-
0.00112
Hordeum vulgare thioredoxin disulfide h1
-
pH 7.4, HvNTR2
-
0.00118
Hordeum vulgare thioredoxin disulfide h1
-
pH 7.4, HvNTR1
-
0.0009
Hordeum vulgare thioredoxin disulfide h2
-
pH 5.7, HvNTR2
-
0.00129
Hordeum vulgare thioredoxin disulfide h2
-
pH 7.4, HvNTR2
-
0.00145
Hordeum vulgare thioredoxin disulfide h2
-
pH 5.7, HvNTR1
-
0.00179
Hordeum vulgare thioredoxin disulfide h2
-
pH 7.4, HvNTR1
-
0.0019
Lipoamide
-
isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C
0.0043
Lipoamide
-
isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C
2.89
Lipoamide
wild type enzyme, in TE buffer, at 20°C
3.68
Lipoamide
mutant enzyme Y116I, in TE buffer, at 20°C
5.59
Lipoamide
mutant enzyme Y116T, in TE buffer, at 20°C
0.011
NADH
-
0.023
NADH
-
with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.0302
NADH
-
in 50 mM potassium phosphate buffer (pH 7.0), at 22°C
0.073
NADH
-
at pH 9.5 and 80°C
0.0806
NADH
truncated mutant, pH 7.2, temperature not specified in the publication
0.1
NADH
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
0.736
NADH
-
cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication
0.0004
NADPH
-
0.001
NADPH
-
cytosolic and mitochondrial TrxR1 isoform
0.0013
NADPH
pH 8.0, 30°C
0.0014
NADPH
-
pH 7.5, 50°C
0.0017
NADPH
pH 5.5, 60°C
0.0018
NADPH
-
with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.0022
NADPH
pH 7.0, 30°C
0.00235
NADPH
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
0.0045
NADPH
-
pH 7.0, 25°C, cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid)
0.0056
NADPH
pH 7.2, temperature not specified in the publication
0.006
NADPH
-
pH 7.8, 37°C
0.0063
NADPH
-
cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication
0.0125
NADPH
-
in 50 mM potassium phosphate buffer (pH 7.0), at 22°C
0.0224
NADPH
pH 7.2, temperature not specified in the publication
0.0247
NADPH
truncated mutant, pH 7.2, temperature not specified in the publication
0.088
NADPH
-
wild type enzyme
0.78
NADPH
-
at pH 6.5 and 80°C
1.1
NADPH
-
TrxR-16 mutant K29R/H108Y/A119N/V478E
2.4
NADPH
-
TrxR-16, TrxR lacking the last 16 C-terminal amino acids
3.3
NADPH
-
TrxR-16 mutant K29R
4.5
NADPH
-
TrxR-16 mutant K29R/H108Y
0.0025
reductase
-
thioredoxin from rat, bovine substrate
-
0.00031
thioredoxin
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
0.0004
thioredoxin
-
SeC498C mutant, human thioredoxin
0.00076
thioredoxin
assay with DTNB instead of NADPH
0.00076
thioredoxin
-
substrate: thioredoxin from E. coli
0.00088
thioredoxin
-
mutant C136S
0.0011
thioredoxin
-
recombinant enzyme
0.00125
thioredoxin
-
wild-type
0.00141
thioredoxin
-
rat thioredoxin as substrate
0.00157
thioredoxin
-
Escherichia coli thioredoxin as substrate
0.0017
thioredoxin
-
at 4°C
0.0017
thioredoxin
-
mutant C139S
0.00183
thioredoxin
-
human thioredoxin as substrate
0.002
thioredoxin
-
substrate wild-type, thioredoxin P34S, pH 8.0
0.00236
thioredoxin
-
human thioredoxin as substrate
0.0025
thioredoxin
-
substrate: thioredoxin from calf and rat
0.00256
thioredoxin
-
Escherichia coli thioredoxin as substrate
0.0028
thioredoxin
-
at 25°C
0.0037
thioredoxin
-
thioredoxin from rat
0.0047
thioredoxin
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.0051
thioredoxin
-
rat thioredoxin as substrate
0.006
thioredoxin
-
human thioredoxin
0.0062
thioredoxin
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.007
thioredoxin
-
cytosolic TrxR1 isoform
0.0085
thioredoxin
-
thioredoxin-1 from Anopheles gambiae, pH 7.4
0.009
thioredoxin
-
thioredoxin-2 from Drosophila melanogaster, pH 7.4
0.0144
thioredoxin
-
substrate: thioredoxin from E. coli
0.019
thioredoxin
-
mitochondrial TrxR1 isoform
0.033
thioredoxin
-
thioredoxin-1 from Plasmodium falciparum, pH 7.4
0.034
thioredoxin
-
thioredoxin from E. coli
0.036
thioredoxin
wild-type, thioredoxin from E. coli
0.0441
thioredoxin
pH 5.5, 60°C
0.045
thioredoxin
-
substrate: thioredoxin from E. coli
0.07
thioredoxin
-
E. coli thioredoxin
0.077
thioredoxin
-
thioredoxin from Escherichia coli, mutant TR-GCCS
0.1047
thioredoxin
-
in 100 mM potassium phosphate (pH 7.0), at 22°C
0.219
thioredoxin
-
thioredoxin from Escherichia coli, mutant TR-SCCS
0.61
thioredoxin
-
thioredoxin from Escherichia coli, wild type enzyme
6.6
thioredoxin
mutant enzyme U489C
35
thioredoxin
semisynthetic enzyme with 63% content of selenium
67.6
thioredoxin
semisynthetic enzyme with 91% content of selenium
0.0006
thioredoxin 2
-
wild type enzyme, pH and temperature not specified in the publication
-
0.0009
thioredoxin 2
-
mutant enzyme K137A, pH and temperature not specified in the publication
-
0.0023
thioredoxin 41
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
0.0036
thioredoxin 41
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
0.0028
thioredoxin 8
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
0.0029
thioredoxin 8
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
0.00099
thioredoxin disulfide
mutant enzyme Y116I, in TE buffer, at 20°C
0.00106
thioredoxin disulfide
mutant enzyme Y116T, in TE buffer, at 20°C
0.00143
thioredoxin disulfide
wild type enzyme, in TE buffer, at 20°C
0.0021
thioredoxin disulfide
pH 7.0, 30°C
0.0032
thioredoxin disulfide
purified, recombinant, tetrameric enzyme
0.0036
thioredoxin disulfide
purified, recombinant, dimeric enzyme
0.00637
thioredoxin disulfide
-
full length enzyme, in 50 mM potassium phosphate pH 7.0, at 25°C
0.0067
thioredoxin disulfide
pH 7.0, 30°C
0.017
thioredoxin disulfide
-
-
0.032
thioredoxin disulfide
full-length mouse enzyme with C-terminal sequence SCCS
0.0676
thioredoxin disulfide
full-length mouse enzyme with C-terminal sequence GCUG
0.086
thioredoxin disulfide
-
Methanosarcina acetivorans thioredoxin 7, cosubstrate: NADPH, pH and temperature not specified in the publication
0.123
thioredoxin disulfide
full-length mouse enzyme with C-terminal sequence GCCG
0.141
thioredoxin disulfide
full-length Drosophila enzyme with C-terminal sequence SCCS
0.173
thioredoxin disulfide
-
pH 7, 25°C
0.007
thioredoxin-1 disulfide
-
TrxR(cyto)
-
0.019
thioredoxin-1 disulfide
-
TRxR(mito)
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
additionally to the Arabidopsis thaliana thioredoxin, several other thioredoxins have been tested, Km-values
-
additional information
additional information
-
Km-values are pH-dependent
-
additional information
additional information
-
effects of paraquat on reaction kinetics of NADH and NADPH in lung cell extracts
-
additional information
additional information
KM-values of semisynthetic truncated mTR3 enzymes
-
additional information
additional information
-
KM-values of semisynthetic truncated mTR3 enzymes
-
additional information
additional information
-
KM-values of truncated enzymes forms
-