1.8.1.9: thioredoxin-disulfide reductase
This is an abbreviated version!
For detailed information about thioredoxin-disulfide reductase, go to the full flat file.
Word Map on EC 1.8.1.9
-
1.8.1.9
-
selenium
-
selenoproteins
-
selenocysteine
-
gsh
-
peroxiredoxins
-
dismutase
-
catalase
-
auranofin
-
glutaredoxins
-
trx1
-
selenoenzyme
-
hydroperoxide
-
peroxidases
-
fad
-
ribonucleotide
-
redox-active
-
cisplatin
-
dithiols
-
goldi
-
se-deficient
-
dtnb
-
iodothyronine
-
flavoenzyme
-
ebselen
-
redox-sensitive
-
selenium-containing
-
selenols
-
deiodinases
-
thiol-dependent
-
organoselenium
-
thioredoxin-like
-
selenium-deficient
-
se-dependent
-
n-heterocyclic
-
thioredoxin-dependent
-
diselenide
-
redox-regulated
-
selenium-dependent
-
thiol-disulfide
-
thiol-based
-
thioredoxin-1
-
ask1
-
carbene
-
1-chloro-2,4-dinitrobenzene
-
sulforaphane
-
secis
-
txnip
-
trypanothione
-
medicine
-
analysis
-
na2seo3
-
agriculture
-
thioredoxin-interacting
- 1.8.1.9
- selenium
-
selenoproteins
- selenocysteine
- gsh
- peroxiredoxins
- dismutase
- catalase
- auranofin
- glutaredoxins
- trx1
-
selenoenzyme
- hydroperoxide
- peroxidases
- fad
- ribonucleotide
-
redox-active
- cisplatin
- dithiols
-
goldi
-
se-deficient
- dtnb
-
iodothyronine
-
flavoenzyme
- ebselen
-
redox-sensitive
-
selenium-containing
-
selenols
-
deiodinases
-
thiol-dependent
-
organoselenium
-
thioredoxin-like
-
selenium-deficient
-
se-dependent
-
n-heterocyclic
-
thioredoxin-dependent
- diselenide
-
redox-regulated
-
selenium-dependent
-
thiol-disulfide
-
thiol-based
- thioredoxin-1
- ask1
-
carbene
- 1-chloro-2,4-dinitrobenzene
- sulforaphane
-
secis
-
txnip
- trypanothione
- medicine
- analysis
- na2seo3
- agriculture
-
thioredoxin-interacting
Reaction
Synonyms
all0737, ApTR, At2g41680, AtNTRA, AtNTRB, bacillithiol disulfide reductase, bacillithiol-disulfide reductase, BBOV_I002190, Bdr, BSSB reductase, CeTR2, DmTR, DmTrxR, DmTrxR-1, EC 1.6.4.5, EhTRXR, ferredoxin:thioredoxin reductase, FTR, general stress protein 35, GSP35, HCOI_01258400, hemolysate thioredoxin reductase, hemolysate TR, hTrxR, HvNTR1, HvNTR2, L-TR, L-TrxR, More, MtNTRC, mTR3, multifunctional thioredoxin-glutathione reductase, NAD(P)H:paraquat oxidoreductase, NADP-dependent thioredoxin reductase, NADP-linked thioredoxin reductase, NADP-thioredoxin reductase, NADP-thioredoxin reductase C, NADPH thioredoxin reductase, NADPH thioredoxin reductase C, NADPH-dependent thioredoxin reductase, NADPH-dependent thioredoxin reductase 2, NADPH-dependent thioredoxin reductase C, NADPH-dependent thioredoxin reductase I, NADPH-dependent thioredoxin reductase-1, NADPH-dependent TRX reductase, NADPH-thioredoxin reductase, NADPH-thioredoxin reductase C, NADPH-Trx reductase, NADPH2:oxidized thioredoxin oxidoreductase, NAPDH-dependent BSSB reductase, NTR, Ntr1, NTR2, Ntr3, NTRA, NTRAB, NtrB, NTRC, PH0178, PH1426, PhRP, PhTrxR, protein-disulfide oxidoreductase, reductase, thioredoxin, SEP1, taTrxR, TGR, thioredoxin glutathione reductase, thioredoxin h reductase, thioredoxin reductase, thioredoxin reductase (NADPH), thioredoxin reductase 1, thioredoxin reductase 2, thioredoxin reductase-1, thioredoxin reductase1, TON_1603, TR, TR1, TR2, TR3, TRase, TrR, Trr1, Trr1p, TRR2, Trx1, Trx3, TrxB, TrxB1, TrxB2, TrxR, TrxR-1(cyto), TrxR-1(mito), TrxR1, TrxR2, TrxR3, TrxRB3, TRXRD, TrxRh1, TrxRh2, TrxT, Txnrd1, TXNRD1-v3, Txnrd2, TXNRD3, TxrR-1, YpdA
ECTree
1.8.1.9 thioredoxin-disulfide reductaseAdvanced search results
results (
results (KM Value
KM Value on EC 1.8.1.9 - thioredoxin-disulfide reductase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
1.25
dithionitrobenzene
-
pH and temperature not specified in the publication
0.0275
ebsulfur disulfide
-
pH 7.5, temperature not specified in the publication
2.5
H2O2
-
wild-type enzyme, Km-value can be reduced by addition of selenocysteine
0.00123
Hordeum vulgare thioredoxin 1
wild-type, pH 7.5, temperature not specified in the publication
-
0.0014
Hordeum vulgare thioredoxin 2 mutant G57P
wild-type, pH 7.5, temperature not specified in the publication
-
0.0155
Hordeum vulgare thioredoxin 2 mutant I51G
wild-type, pH 7.5, temperature not specified in the publication
-
0.0027
lipoic acid
-
isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C
0.0013
thioredoxin 1
-
wild type enzyme, pH and temperature not specified in the publication
0.0011
thioredoxin 3
-
wild type enzyme, pH and temperature not specified in the publication
-
2.4
5,5'-dithio-bis(2-nitrobenzoic acid)
pH 7.2, temperature not specified in the publication
2.5
5,5'-dithio-bis(2-nitrobenzoic acid)
pH 7.2, temperature not specified in the publication
11.9
5,5'-dithio-bis(2-nitrobenzoic acid)
truncated mutant, pH 7.2, temperature not specified in the publication
0.0186
5,5'-dithiobis(2-nitrobenzoic acid)
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
0.09
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, 10 mM potassium phosphate buffer (pH 7.0) containing 10 mM EDTA, at 25°C
0.0906
5,5'-dithiobis(2-nitrobenzoic acid)
mutant enzyme Y116T, in TE buffer, at 20°C
0.094
5,5'-dithiobis(2-nitrobenzoic acid)
wild type enzyme, in TE buffer, at 20°C
0.0971
5,5'-dithiobis(2-nitrobenzoic acid)
mutant enzyme Y116I, in TE buffer, at 20°C
0.105
5,5'-dithiobis(2-nitrobenzoic acid)
-
isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C
0.114
5,5'-dithiobis(2-nitrobenzoic acid)
-
full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C
0.139
5,5'-dithiobis(2-nitrobenzoic acid)
purified, recombinant, tetrameric enzyme
0.17
5,5'-dithiobis(2-nitrobenzoic acid)
full-length Drosophila enzyme with C-terminal sequence SCCS
0.2156
5,5'-dithiobis(2-nitrobenzoic acid)
purified, recombinant, dimeric enzyme
0.22
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
0.39
5,5'-dithiobis(2-nitrobenzoic acid)
-
isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C
0.41
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
0.45
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
0.463
5,5'-dithiobis(2-nitrobenzoic acid)
-
in 50 mM potassium phosphate buffer (pH 7.0), at 22°C
0.47
5,5'-dithiobis(2-nitrobenzoic acid)
full-length mouse enzyme with C-terminal sequence GCUG
0.53
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
0.71
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C
0.83
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
0.92
5,5'-dithiobis(2-nitrobenzoic acid)
truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid
1.1
5,5'-dithiobis(2-nitrobenzoic acid)
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
1.4
5,5'-dithiobis(2-nitrobenzoic acid)
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
2.4
5,5'-dithiobis(2-nitrobenzoic acid)
-
TrxR-16, TrxR lacking the last 16 C-terminal amino acids
2.72
5,5'-dithiobis(2-nitrobenzoic acid)
truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid
4.1
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
0.00227
5-hydroxy-1,4-naphthoquinone
wild type enzyme, in TE buffer, at 20°C
0.00698
5-hydroxy-1,4-naphthoquinone
mutant enzyme Y116T, in TE buffer, at 20°C
0.02374
5-hydroxy-1,4-naphthoquinone
mutant enzyme Y116I, in TE buffer, at 20°C
0.00048
Arabidopsis thaliana thioredoxin 3
pH 7.5, temperature not specified in the publication
-
0.00052
Arabidopsis thaliana thioredoxin 3
mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication
-
0.00067
Arabidopsis thaliana thioredoxin 3
mutant G225R/G226D, pH 7.5, temperature not specified in the publication
-
0.00073
Arabidopsis thaliana thioredoxin 3
mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication
-
0.0008
Arabidopsis thaliana thioredoxin 3
wild-type, pH 7.5, temperature not specified in the publication
-
0.0024
Arabidopsis thaliana thioredoxin 3
mutant W42A, pH 7.5, temperature not specified in the publication
-
0.0042
Arabidopsis thaliana thioredoxin 3
mutant N45A/D46A, pH 7.5, temperature not specified in the publication
-
0.0141
Arabidopsis thaliana thioredoxin 3
mutant M43A, pH 7.5, temperature not specified in the publication
-
0.0305
Arabidopsis thaliana thioredoxin 3
mutant W42A/M43A, pH 7.5, temperature not specified in the publication
-
0.0543
Arabidopsis thaliana thioredoxin 3
mutant Delta42-47, pH 7.5, temperature not specified in the publication
-
0.149
Escherichia coli thioredoxin
wild-type, pH 7.5, temperature not specified in the publication
-
0.00068
Hordeum vulgare thioredoxin 2
mutant N139A, pH 7.5, temperature not specified in the publication
-
0.00105
Hordeum vulgare thioredoxin 2
mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication
-
0.00117
Hordeum vulgare thioredoxin 2
wild-type, pH 7.5, temperature not specified in the publication
-
0.00128
Hordeum vulgare thioredoxin 2
mutant G225R/G226D, pH 7.5, temperature not specified in the publication
-
0.00146
Hordeum vulgare thioredoxin 2
mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication
-
0.0022
Hordeum vulgare thioredoxin 2
mutant N45A/D46A, pH 7.5, temperature not specified in the publication
-
0.00343
Hordeum vulgare thioredoxin 2
mutant R140A, pH 7.5, temperature not specified in the publication
-
0.016
Hordeum vulgare thioredoxin 2
mutant M43A, pH 7.5, temperature not specified in the publication
-
0.0457
Hordeum vulgare thioredoxin 2
mutant W42A, pH 7.5, temperature not specified in the publication
-
0.06
Hordeum vulgare thioredoxin 2
pH 7.5, temperature not specified in the publication
-
0.00041
Hordeum vulgare thioredoxin 2 mutant E86A
mutant N139A, pH 7.5, temperature not specified in the publication
-
0.00057
Hordeum vulgare thioredoxin 2 mutant E86A
wild-type, pH 7.5, temperature not specified in the publication
-
0.0016
Hordeum vulgare thioredoxin 2 mutant E86A
mutant R140A, pH 7.5, temperature not specified in the publication
-
0.023
NADH
-
with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.0806
NADH
truncated mutant, pH 7.2, temperature not specified in the publication
0.1
NADH
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
0.736
NADH
-
cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication
0.0018
NADPH
-
with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.00235
NADPH
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
0.0045
NADPH
-
pH 7.0, 25°C, cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid)
0.0056
NADPH
pH 7.2, temperature not specified in the publication
0.0063
NADPH
-
cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication
0.0224
NADPH
pH 7.2, temperature not specified in the publication
0.0247
NADPH
truncated mutant, pH 7.2, temperature not specified in the publication
0.00031
thioredoxin
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
0.0047
thioredoxin
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.0062
thioredoxin
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.61
thioredoxin
-
thioredoxin from Escherichia coli, wild type enzyme
0.0006
thioredoxin 2
-
wild type enzyme, pH and temperature not specified in the publication
-
0.0009
thioredoxin 2
-
mutant enzyme K137A, pH and temperature not specified in the publication
-
0.0023
thioredoxin 41
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
0.0036
thioredoxin 41
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
0.0028
thioredoxin 8
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
0.0029
thioredoxin 8
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
0.00099
thioredoxin disulfide
mutant enzyme Y116I, in TE buffer, at 20°C
0.00106
thioredoxin disulfide
mutant enzyme Y116T, in TE buffer, at 20°C
0.00143
thioredoxin disulfide
wild type enzyme, in TE buffer, at 20°C
0.0032
thioredoxin disulfide
purified, recombinant, tetrameric enzyme
0.00637
thioredoxin disulfide
-
full length enzyme, in 50 mM potassium phosphate pH 7.0, at 25°C
0.032
thioredoxin disulfide
full-length mouse enzyme with C-terminal sequence SCCS
0.0676
thioredoxin disulfide
full-length mouse enzyme with C-terminal sequence GCUG
0.086
thioredoxin disulfide
-
Methanosarcina acetivorans thioredoxin 7, cosubstrate: NADPH, pH and temperature not specified in the publication
0.123
thioredoxin disulfide
full-length mouse enzyme with C-terminal sequence GCCG
0.141
thioredoxin disulfide
full-length Drosophila enzyme with C-terminal sequence SCCS
additional information
additional information
-
additionally to the Arabidopsis thaliana thioredoxin, several other thioredoxins have been tested, Km-values
-
additional information
additional information
-
Km-values are pH-dependent
-
additional information
additional information
-
effects of paraquat on reaction kinetics of NADH and NADPH in lung cell extracts
-
additional information
additional information
KM-values of semisynthetic truncated mTR3 enzymes
-
additional information
additional information
-
KM-values of semisynthetic truncated mTR3 enzymes
-
additional information
additional information
-
KM-values of truncated enzymes forms
-
