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0.88 - 87.8
5,5'-dithio-bis(2-nitrobenzoic acid)
0.012 - 106.3
5,5'-dithiobis(2-nitrobenzoic acid)
24.1 - 174.3
5-hydroxy-1,4-naphthoquinone
3.9 - 15.3
Arabidopsis thaliana thioredoxin 3
-
20
Babesia bovis thioredoxin
pH 8.0, 30°C
-
11.7
ebsulfur
-
pH 7.5, temperature not specified in the publication
1.7
ebsulfur disulfide
-
pH 7.5, temperature not specified in the publication
4.1 - 9.4
Escherichia coli thioredoxin
-
12.1
Hordeum vulgare thioredoxin 1
wild-type, pH 7.5, temperature not specified in the publication
-
1.8 - 11.4
Hordeum vulgare thioredoxin 2
-
2.6 - 5.3
Hordeum vulgare thioredoxin 2 mutant E86A
-
7.9
Hordeum vulgare thioredoxin 2 mutant G57P
wild-type, pH 7.5, temperature not specified in the publication
-
15.9
Hordeum vulgare thioredoxin 2 mutant I51G
wild-type, pH 7.5, temperature not specified in the publication
-
2.25 - 3.26
Hordeum vulgare thioredoxin disulfide h1
-
0.43 - 2.98
Hordeum vulgare thioredoxin disulfide h2
-
1.183 - 6.08
hydrogen peroxide
1.8
lipoic acid
-
isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C
16.05
methaneseleninic acid
-
at pH 6.1 and 37°C
500
protein disulfide-isomerase
-
-
-
1.83
rat thioredoxin
-
insulin coupled assay
-
43.7
thioredoxin 1
-
wild type enzyme, pH and temperature not specified in the publication
42.9 - 47.1
thioredoxin 2
-
34
thioredoxin 3
-
wild type enzyme, pH and temperature not specified in the publication
-
2.6 - 2.7
thioredoxin 8
-
0.083 - 114.1
thioredoxin disulfide
2.2
thioredoxin disulfide 41
-
pH 7, 30°C
-
1.43
thioredoxin disulfide 8
-
pH 7, 30°C
-
22.7
thioredoxin K36E, thioredoxin P34S
-
mutant, pH 8.0
-
10.3
thioredoxin-CAC, thioredoxin-R
-
mutant, pH 8.0
-
additional information
additional information
-
0.88
5,5'-dithio-bis(2-nitrobenzoic acid)
pH 7.2, temperature not specified in the publication
1.23
5,5'-dithio-bis(2-nitrobenzoic acid)
truncated mutant, pH 7.2, temperature not specified in the publication
10.7
5,5'-dithio-bis(2-nitrobenzoic acid)
pH 7.2, temperature not specified in the publication
10.8
5,5'-dithio-bis(2-nitrobenzoic acid)
pH 8.0, 30°C
11.96
5,5'-dithio-bis(2-nitrobenzoic acid)
-
pH 7.8, 37°C
87.8
5,5'-dithio-bis(2-nitrobenzoic acid)
-
pH 7.5, 50°C
0.012
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant U498C
0.018
5,5'-dithiobis(2-nitrobenzoic acid)
-
TrxR-16 mutant K29R/H108Y/A119N/V478E
0.075
5,5'-dithiobis(2-nitrobenzoic acid)
-
TrxR-16 mutant K29R/H108Y
0.23
5,5'-dithiobis(2-nitrobenzoic acid)
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.25
5,5'-dithiobis(2-nitrobenzoic acid)
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.52
5,5'-dithiobis(2-nitrobenzoic acid)
-
TrxR-16 mutant K29R
0.55
5,5'-dithiobis(2-nitrobenzoic acid)
-
TrxR-16, TrxR lacking the last 16 C-terminal amino acids
0.62
5,5'-dithiobis(2-nitrobenzoic acid)
-
0.62
5,5'-dithiobis(2-nitrobenzoic acid)
pH 7.5
1.2
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C
1.6
5,5'-dithiobis(2-nitrobenzoic acid)
full-length Drosophila enzyme with C-terminal sequence SCCS
1.77
5,5'-dithiobis(2-nitrobenzoic acid)
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
2.23
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme
2.23
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
2.4
5,5'-dithiobis(2-nitrobenzoic acid)
truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid
2.53
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
2.62
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH 7, 25°C
2.62
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
5.5
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH 7.4
8.28
5,5'-dithiobis(2-nitrobenzoic acid)
-
isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C
9
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
15.6
5,5'-dithiobis(2-nitrobenzoic acid)
truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid
16
5,5'-dithiobis(2-nitrobenzoic acid)
-
full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C
18.73
5,5'-dithiobis(2-nitrobenzoic acid)
-
isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C
20.83
5,5'-dithiobis(2-nitrobenzoic acid)
full-length mouse enzyme with C-terminal sequence GCUG
20.85
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 50 mM potassium phosphate at pH 7.0
21.6
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
30.02
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, 10 mM potassium phosphate buffer (pH 7.0) containing 10 mM EDTA, at 25°C
33.08
5,5'-dithiobis(2-nitrobenzoic acid)
purified, recombinant, tetrameric enzyme
33.33
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme
47.72
5,5'-dithiobis(2-nitrobenzoic acid)
mutant enzyme Y116T, in TE buffer, at 20°C
48.42
5,5'-dithiobis(2-nitrobenzoic acid)
-
truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0
49.87
5,5'-dithiobis(2-nitrobenzoic acid)
mutant enzyme Y116I, in TE buffer, at 20°C
70.3
5,5'-dithiobis(2-nitrobenzoic acid)
wild type enzyme, in TE buffer, at 20°C
106.3
5,5'-dithiobis(2-nitrobenzoic acid)
purified, recombinant, dimeric enzyme
24.1
5-hydroxy-1,4-naphthoquinone
wild type enzyme, in TE buffer, at 20°C
52.75
5-hydroxy-1,4-naphthoquinone
mutant enzyme Y116T, in TE buffer, at 20°C
174.3
5-hydroxy-1,4-naphthoquinone
mutant enzyme Y116I, in TE buffer, at 20°C
3.9
Arabidopsis thaliana thioredoxin 3
mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication
-
4.8
Arabidopsis thaliana thioredoxin 3
wild-type, pH 7.5, temperature not specified in the publication
-
5
Arabidopsis thaliana thioredoxin 3
mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication
-
5
Arabidopsis thaliana thioredoxin 3
mutant G225R/G226D, pH 7.5, temperature not specified in the publication
-
5.9
Arabidopsis thaliana thioredoxin 3
pH 7.5, temperature not specified in the publication
-
10.4
Arabidopsis thaliana thioredoxin 3
mutant W42A, pH 7.5, temperature not specified in the publication
-
10.7
Arabidopsis thaliana thioredoxin 3
mutant N45A/D46A, pH 7.5, temperature not specified in the publication
-
11
Arabidopsis thaliana thioredoxin 3
mutant W42A/M43A, pH 7.5, temperature not specified in the publication
-
14.8
Arabidopsis thaliana thioredoxin 3
mutant M43A, pH 7.5, temperature not specified in the publication
-
15.3
Arabidopsis thaliana thioredoxin 3
mutant Delta42-47, pH 7.5, temperature not specified in the publication
-
0.16
DTNB
-
mutant enzyme H509A
0.233
DTNB
-
mutant enzyme H509Q
4.58
DTNB
-
wild-type enzyme
50.3
DTNB
-
chimeric enzyme mutant, partly from Salmonella typhimurium AhpF protein
4.1
Escherichia coli thioredoxin
pH 8.0, 30°C
-
9.4
Escherichia coli thioredoxin
wild-type, pH 7.5, temperature not specified in the publication
-
1.8
Hordeum vulgare thioredoxin 2
mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication
-
4.2
Hordeum vulgare thioredoxin 2
mutant N139A, pH 7.5, temperature not specified in the publication
-
5.1
Hordeum vulgare thioredoxin 2
mutant R140A, pH 7.5, temperature not specified in the publication
-
7.3
Hordeum vulgare thioredoxin 2
mutant N45A/D46A, pH 7.5, temperature not specified in the publication
-
7.9
Hordeum vulgare thioredoxin 2
mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication
-
8
Hordeum vulgare thioredoxin 2
wild-type, pH 7.5, temperature not specified in the publication
-
8.1
Hordeum vulgare thioredoxin 2
mutant G225R/G226D, pH 7.5, temperature not specified in the publication
-
8.5
Hordeum vulgare thioredoxin 2
pH 7.5, temperature not specified in the publication
-
10.4
Hordeum vulgare thioredoxin 2
mutant W42A, pH 7.5, temperature not specified in the publication
-
11.4
Hordeum vulgare thioredoxin 2
mutant M43A, pH 7.5, temperature not specified in the publication
-
2.6
Hordeum vulgare thioredoxin 2 mutant E86A
mutant R140A, pH 7.5, temperature not specified in the publication
-
4.2
Hordeum vulgare thioredoxin 2 mutant E86A
mutant N139A, pH 7.5, temperature not specified in the publication
-
5.3
Hordeum vulgare thioredoxin 2 mutant E86A
wild-type, pH 7.5, temperature not specified in the publication
-
2.25
Hordeum vulgare thioredoxin disulfide h1
-
pH 7.4, HvNTR1
-
3.26
Hordeum vulgare thioredoxin disulfide h1
-
pH 7.4, HvNTR2
-
0.43
Hordeum vulgare thioredoxin disulfide h2
-
pH 5.7, HvNTR1
-
0.8
Hordeum vulgare thioredoxin disulfide h2
-
pH 5.7, HvNTR2
-
1.31
Hordeum vulgare thioredoxin disulfide h2
-
pH 7.4, HvNTR1
-
2.98
Hordeum vulgare thioredoxin disulfide h2
-
pH 7.4, HvNTR2
-
1.183
hydrogen peroxide
semisynthetic enzyme with 91% content of selenium
6.08
hydrogen peroxide
semisynthetic enzyme with 91% content of selenium
1.6
Lipoamide
-
isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C
2 - 3.7
Lipoamide
wild type enzyme, in TE buffer, at 20°C
3.3
Lipoamide
-
isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C
27.6
Lipoamide
mutant enzyme Y116I, in TE buffer, at 20°C
31.2
Lipoamide
mutant enzyme Y116T, in TE buffer, at 20°C
12
methylseleninate
-
recombinant protein
0.06
NADH
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
0.2
NADH
-
with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.2
NADH
truncated mutant, pH 7.2, temperature not specified in the publication
0.817
NADH
-
cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication
0.25
NADPH
-
with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
0.35
NADPH
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
0.59
NADPH
pH 7.2, temperature not specified in the publication
0.65
NADPH
-
cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication
0.97
NADPH
truncated mutant, pH 7.2, temperature not specified in the publication
6.4
NADPH
pH 7.2, temperature not specified in the publication
109.8
NADPH
-
pH 7.5, 50°C
0.016
thioredoxin
-
Escherichia coli thioredoxin as substrate
0.02
thioredoxin
mutant enzyme U489C
0.052 - 2.1
thioredoxin
-
thioredoxin from Escherichia coli, wild type enzyme
0.243
thioredoxin
-
SeC498C mutant enzyme, human thioredoxin
0.38
thioredoxin
-
in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication
0.5
thioredoxin
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
1.25
thioredoxin
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
1.3
thioredoxin
pH 5.5, 60°C
2.38
thioredoxin
-
mutant C136S
3.5
thioredoxin
-
human thioredoxin as substrate
4.03
thioredoxin
-
thioredoxin from Escherichia coli, mutant TR-SCCS
5.3
thioredoxin
-
thioredoxin from Escherichia coli, mutant TR-GCCS
5.58
thioredoxin
-
insulin coupled assay method
8.1
thioredoxin
-
human thioredoxin as substrate
10.17
thioredoxin
-
thioredoxin from Escherichia coli, wild type enzyme
13.2
thioredoxin
-
mutant C139S
14.3
thioredoxin
-
thioredoxin-2 from Drosophila melanogaster, pH 7.4
15.4
thioredoxin
-
thioredoxin-1 from Anopheles gambiae, pH 7.4
15.7
thioredoxin
-
thioredoxin-1 from Plasmodium falciparum, pH 7.4
19.97
thioredoxin
-
human thioredoxin as substrate
22
thioredoxin
-
wild-type
22.8
thioredoxin
-
wild-type, pH 8.0
25
thioredoxin
semisynthetic enzyme with 63% content of selenium
25.78
thioredoxin
-
human thioredoxin as substrate
27.4
thioredoxin
-
rat thioredoxin as substrate
29.5
thioredoxin
-
Escherichia coli thioredoxin as substrate
37
thioredoxin
semisynthetic enzyme with 91% content of selenium
37.88
thioredoxin
-
rat thioredoxin as substrate
41.7
thioredoxin
-
wild-type enzyme, human thioredoxin
46.57
thioredoxin
-
Escherichia coli thioredoxin as substrate
1030
thioredoxin
-
human thioredoxin
1300
thioredoxin
-
thioredoxin from E. coli
42.9
thioredoxin 2
-
wild type enzyme, pH and temperature not specified in the publication
-
47.1
thioredoxin 2
-
mutant enzyme K137A, pH and temperature not specified in the publication
-
2
thioredoxin 41
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
2.2
thioredoxin 41
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
2.6
thioredoxin 8
-
with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
2.7
thioredoxin 8
-
with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C
-
0.083
thioredoxin disulfide
full-length mouse enzyme with C-terminal sequence SCCS
0.13
thioredoxin disulfide
full-length mouse enzyme with C-terminal sequence GCCG
1.175
thioredoxin disulfide
-
Methanosarcina acetivorans thioredoxin 7, cosubstrate: NADPH, pH and temperature not specified in the publication
4.99
thioredoxin disulfide
-
pH 7, 25°C
5.8
thioredoxin disulfide
full-length Drosophila enzyme with C-terminal sequence SCCS
7.7
thioredoxin disulfide
pH 7.0, 30°C
13.3
thioredoxin disulfide
pH 7.0, 30°C
19.2
thioredoxin disulfide
-
-
30
thioredoxin disulfide
-
full length enzyme, in 50 mM potassium phosphate pH 7.0, at 25°C
37
thioredoxin disulfide
full-length mouse enzyme with C-terminal sequence GCUG
40.98
thioredoxin disulfide
mutant enzyme Y116I, in TE buffer, at 20°C
44.85
thioredoxin disulfide
mutant enzyme Y116T, in TE buffer, at 20°C
47 - 73
thioredoxin disulfide
purified, recombinant, tetrameric enzyme
94.17
thioredoxin disulfide
wild type enzyme, in TE buffer, at 20°C
114.1
thioredoxin disulfide
purified, recombinant, dimeric enzyme
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
wild-type and recombinant chimeric mutants in a coupled assay
-
additional information
additional information
-
transhydrogenase activity of mutants and wild-type enzyme with thioredoxin and FAD
-
additional information
additional information
turnover number of semisynthetic truncated mTR3 enzymes
-
additional information
additional information
-
turnover number of semisynthetic truncated mTR3 enzymes
-
additional information
additional information
-
turnover number of truncated enzyme forms
-