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1.8.2.2: thiosulfate dehydrogenase

This is an abbreviated version!
For detailed information about thiosulfate dehydrogenase, go to the full flat file.

Word Map on EC 1.8.2.2

Reaction

2 thiosulfate +

2 ferricytochrome c
=
tetrathionate
 + 2 ferrocytochrome c + 4 H+

Synonyms

AFE_0042, Alvin_0091, AvTsdA, C8J_0815, D0Y83_01395, di-heme TsdA, DIE28_04650, diheme cytochrome c TsdA, enzymes, thiosulfate-oxidizing, MpTsdBA, oxidase, thiosulfate, Tat pathway signal sequence domain protein, tetrathionate reductase, tetrathionate synthase, thiosulfate dehydrogenase, thiosulfate oxidase, thiosulfate-acceptor oxidoreductase, thiosulfate-oxidizing enzyme, TSD, TsdA

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.2 With a cytochrome as acceptor
                1.8.2.2 thiosulfate dehydrogenase

Purification

Purification on EC 1.8.2.2 - thiosulfate dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography with immobilized cytochrome c
-
partial
partial, using ion exchange chromatography, gel filtration and hydrophobic interaction chromatography
-
partial, using ion-exchange chromatography and calcium phosphate gel treatment
-
partial, using ultracentrifugation, ammonium sulfate fractionation, column chromatography on DEAE-cellulose and gel filtration on Sephacryl S-200
-
partial, using ultracentrifugation, ultrafiltration, column chromatography on DEAE-Sephadex A-25 and gel filtration on Sephadex S-200
-
purified preparation is brwon in color
-
recombinant Strep-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography and gel filtration
recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity chromatography and gel filtration
using a Phenyl-Sepharose, a DEAE Sephacel and a Macroprep ceramic hydroxyapatite type I, and a Mono Q column
-
using ammonium sulfate precipitation, hydrophobic interaction chromatography, anion-exchange chromatography and gel filtration
-
using chromatography on Phenyl-Sepharose column and Mono Q column, gel filtration on Superdex 200 and column chromatography on Phenyl Superose
-
using heat-treatment, 5-17% ethanol precipitation and CM-cellulose treatment
-
using MnCl2 precipitation, treatment with calcium phosphate gel, and successive column chromatography on DEAE-Sephadex and Sephadex G-150
-
using sonication, column chromatography on DEAE-cellulose, pH-adjustment to 4.5 and calcium phosphate gel treatment
-