1.8.5.8: eukaryotic sulfide quinone oxidoreductase
This is an abbreviated version!
For detailed information about eukaryotic sulfide quinone oxidoreductase, go to the full flat file.
Reaction
Synonyms
glutathione:CoQ reductase, HMT2, ScSQR, SQOR, SQR, Sqrdl, sulfide : quinone oxidoreductase, sulfide quinone oxidoreductase, sulfide:quinone oxidoreductase
ECTree
1.8.5.8 eukaryotic sulfide quinone oxidoreductaseAdvanced search results
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results (Crystallization
Crystallization on EC 1.8.5.8 - eukaryotic sulfide quinone oxidoreductase
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CRYSTALLIZATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
purified selenomethionine-substituted and native enzyme, X-ray diffraction structure determination analysis at 2.59 and 2.99 A resolution, respectively, the SeMet and native structures are essentially identical
purified SQOR-CoQ1 complexed with cyanide, hanging drop vapor diffusion method, mixing of 17.4 mg/ml solubilized human SQOR in 50 mM Tris-HCl, pH 8.0, 300 mM NaCl, 0.05% w/v n-dodecyl beta-D-maltoside, and 5 mM CoQ1 (in 100% DMSO), with reservoir solution composed of 200 mM ammonium tartrate dibasic, pH 6.6, and 20% w/v PEG 3350, yielding a final CoQ1 concentration of 2.5 mM, in a 1:1 ratio, at 20°C, soaking of crystals with 10 mM potassium cyanide solution for 45 min, X-ray diffraction structure determination analysis at 2.25 A resolution, molecular replacement using a SQOR-CoQ1 structure (PDB ID 6OIB) as a search model, structure modeling
purified SQR in the absence and presence of CoQ1, a soluble CoQ10 analogue, and with/without sulfite or sulfide, X-ray diffraction structure determination and analysis at 2.0-2.8 A resolution. Soaking SQR-CoQ1 crystals with sodium sulfide provided evidence for in crystallo catalysis and captures CoQ1 exiting a long hydrophobic channel leading from FAD to the putative membrane-facing surface of SQR
