1.8.7.1: assimilatory sulfite reductase (ferredoxin)
This is an abbreviated version!
For detailed information about assimilatory sulfite reductase (ferredoxin), go to the full flat file.
Word Map on EC 1.8.7.1
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1.8.7.1
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nitrite
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maize
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4.2.99.8
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o-acetyl-l-serine
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2.7.7.4
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sulfhydrylase
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cyanidioschyzon
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sulfurylase
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sulphite
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5'-phosphosulfate
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merolae
-
viologen
-
medicine
- 1.8.7.1
- nitrite
- maize
-
4.2.99.8
- o-acetyl-l-serine
-
2.7.7.4
-
sulfhydrylase
-
cyanidioschyzon
-
sulfurylase
- sulphite
- 5'-phosphosulfate
- merolae
- viologen
- medicine
Reaction
+ 3 oxidized ferredoxin + 3 H2O = + 3 reduced ferredoxin + 6 H+
Synonyms
CMG021C, CmSiRB, coenzyme F420-dependent sulfite reductase, CYME_CMG021C, CYME_CMJ117C, DCP68, desulphoviridin, dissimilatory sulfite reductase, dSiR, EC 1.8.99.1, Fd-SiR, FdSiR, ferredoxin and sulfite reductase, ferredoxin sulfite reductase, ferredoxin-dependent sulfite reductase, ferredoxin-sulfite reductase, ferredoxin:sulfite oxidoreductase, ferredoxin:sulfite reductase, Fsr, NirA, PsSiR, SIR, SiRA, SirB, siroheme- and [Fe4-S4]-dependent NirA, sulfite reductase, sulfite reductase B, ZmSiR
ECTree
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Purification
Purification on EC 1.8.7.1 - assimilatory sulfite reductase (ferredoxin)
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cell extract is treated with DEAE-cellulose in a batchwise manner, the unbound fraction is fractionated by ammonium sulfate precipitation, anion-exchange and hydrophobic column chromatography are applied
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Ni2+-nitrilotriacetic acid affinity matrix and Q-Sepharose column chromatography
phenyl-Sepharose chromatography, F420-Sepharose chromatography and QAE-Sephadex gel filtration
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recombinant His-tagged wild-type and mutant enzymes, as well as enzyme CysG, by alternating ultrafiltration and nickel affinity chromatography, followed by anion exchange chromatography, ultrafiltration, and gel filtration