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1.8.7.1: assimilatory sulfite reductase (ferredoxin)

This is an abbreviated version!
For detailed information about assimilatory sulfite reductase (ferredoxin), go to the full flat file.

Word Map on EC 1.8.7.1

Reaction

hydrogen sulfide
+ 3 oxidized ferredoxin + 3 H2O =
sulfite
+ 3 reduced ferredoxin + 6 H+

Synonyms

CMG021C, CmSiRB, coenzyme F420-dependent sulfite reductase, CYME_CMG021C, CYME_CMJ117C, DCP68, desulphoviridin, dissimilatory sulfite reductase, dSiR, EC 1.8.99.1, Fd-SiR, FdSiR, ferredoxin and sulfite reductase, ferredoxin sulfite reductase, ferredoxin-dependent sulfite reductase, ferredoxin-sulfite reductase, ferredoxin:sulfite oxidoreductase, ferredoxin:sulfite reductase, Fsr, NirA, PsSiR, SIR, SiRA, SirB, siroheme- and [Fe4-S4]-dependent NirA, sulfite reductase, sulfite reductase B, ZmSiR

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.7 With an iron-sulfur protein as acceptor
                1.8.7.1 assimilatory sulfite reductase (ferredoxin)

Purification

Purification on EC 1.8.7.1 - assimilatory sulfite reductase (ferredoxin)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitacion, filtration on a superdex-75 column
-
cell extract is treated with DEAE-cellulose in a batchwise manner, the unbound fraction is fractionated by ammonium sulfate precipitation, anion-exchange and hydrophobic column chromatography are applied
-
Ni2+-nitrilotriacetic acid affinity matrix and Q-Sepharose column chromatography
partially, crude leaves extract
-
phenyl-sepharosa column, DNA cellulose column
-
phenyl-Sepharose chromatography, F420-Sepharose chromatography and QAE-Sephadex gel filtration
-
recombinant His-tagged wild-type and mutant enzymes, as well as enzyme CysG, by alternating ultrafiltration and nickel affinity chromatography, followed by anion exchange chromatography, ultrafiltration, and gel filtration