1.8.98.2: sulfiredoxin

This is an abbreviated version!
For detailed information about sulfiredoxin, go to the full flat file.

Word Map on EC 1.8.98.2

Reaction

peroxiredoxin-(S-hydroxy-S-oxocysteine)
+
ATP
+ 2 R-SH =
peroxiredoxin-(S-hydroxycysteine)
+
ADP
+
phosphate
+
R-S-S-R

Synonyms

AtSrx, cysteine-sulfinic acid reductase, neoplastic progression 3, peroxiredoxin-(S-hydroxy-S-oxocysteine) reductase, protein cysteine sulfinic acid reductase, Srx, Srx1, Srxn1, sulfiredoxin, sulfiredoxin 1, sulfiredoxin-1, sulphiredoxin

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.98 With other, known, physiological acceptors
                1.8.98.2 sulfiredoxin

Cloned

Cloned on EC 1.8.98.2 - sulfiredoxin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged wild-type Srx and mutants in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in HEK293 cells and Escherichia coli
expressed in Saccharomyces cerevisiae
expression in Escherichia coli
for expression in Escherichia coli BL21DE3 cells
-
into the vector pcDNA3
-
into the vector pET14b for expression in Escherichia coli BL21 cells
-
the 5' flanking region of the human Srx1 promoter region, -3664 - +19, is ligated into the vector pCR2.1
-
the vectors pcDNA3 and pFLAG-CMV2 are used, a leader sequence is cloned into the N-terminus coding region of Srx
-
transient expression of an AtSrx-GFP fusion in Nicotiana tabacum leaves