1.8.99.2: adenylyl-sulfate reductase

This is an abbreviated version!
For detailed information about adenylyl-sulfate reductase, go to the full flat file.

Word Map on EC 1.8.99.2

Reaction

AMP
+
sulfite
+
acceptor
=
adenylyl sulfate
+
reduced acceptor

Synonyms

5'-adenylyl sulfate reductase, 5'-adenylylsulfate reductase, 5-adenylylsulfate reductase, AcAPR1, adenosine 5'-phosphosulfate reductase, adenosine phosphosulfate reductase, adenosine-5'-phosphosulfate reductase, adenylyl sulfate reductase, adenylylsulfate reductase, adenylylsulphate reductase, AdoPSO4 reductase, AMP,sulfite:flavin oxidoreductase, APR, APR1, APR2, APR3, AprA, AprB, AprBA, APS reductase, APS-reductase, APSR, AR, ATAPR2, Dde_1110, dissimilatory APS reductase, dissimilatory APS reductases, EAPR, EiAPR, FAD, FeS-enzyme adenosine-5'-phosphosulfate reductase, LMAPR, reductase, adenylylsulfate

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.99 With unknown physiological acceptors
                1.8.99.2 adenylyl-sulfate reductase

Molecular Weight

Molecular Weight on EC 1.8.99.2 - adenylyl-sulfate reductase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
160000
170000
17100
-
alpha2beta2, 2 * 73300 + 2 * 17100, SDS-PAGE
175000
18000
-
1 * 18000 + 1 * 75000, containing one FAD per molecule and eight iron atoms arranged in two [4Fe-4S] clusters, SDS-PAGE
180000
18500
2 * 80000 + 1 * 18500, alpha2beta structure, SDS-PAGE
186000
-
smallest catalytic active subunit, also aggregations of 324000 Da and 569000 Da are detected, gel filtration
190000
-
gel filtration
20000
200000
-
estimation from sedimentation coefficient
210000
-
gel filtration
25600
-
alpha2beta2, 2 * 67800 + 2 * 25600, smallest catalytic active unit, SDS-PAGE
26000
27000
-
2 * 27000, SDS-PAGE
28350
-
calculated average mass, determined on the elemental composition of the apoprotein plus the components in the metal center, assuming that all cysteines are in reduced form and the metal cluster has an oxidation state of zero
28360
-
calculated from amino acid sequence
28513
-
1 * 28513, analytical ultracentrifugation
28560
-
calculated from amino acid sequence
28700
28710
30470
-
MALDI-TOF mass spectrometry, the enzyme used is a recombinant protein with 48 additional residues containing the His6 tag
35000
-
SDS-PAGE
35430
-
mass spectrometry
400000
-
gel filtration
439500
-
equilibrium sedimentation
48000
non-reducing and reducing SDS-PAGE
48700
-
MALDI-TOF mass spectrometry, whole enzyme
48710
-
calculated from sequence of cDNA, whole enzyme
52000
-
SDS-PAGE
600000
-
gel filtration, the majority of the protein is aggregated
67800
-
alpha2beta2, 2 * 67800 + 2 * 25600, smallest catalytic active unit, SDS-PAGE
70000
72000
-
x * 72000 + x * 20000, SDS-PAGE
73300
-
alpha2beta2, 2 * 73300 + 2 * 17100, SDS-PAGE
75000
80000
90000 - 124000
-
dynamic light scattering
96000
non-reducing SDS-PAGE, 16% of the protein was present as a homodimer