1.8.99.2: adenylyl-sulfate reductase

This is an abbreviated version!
For detailed information about adenylyl-sulfate reductase, go to the full flat file.

Word Map on EC 1.8.99.2

Reaction

AMP
+
sulfite
+
acceptor
=
adenylyl sulfate
+
reduced acceptor

Synonyms

5'-adenylyl sulfate reductase, 5'-adenylylsulfate reductase, 5-adenylylsulfate reductase, AcAPR1, adenosine 5'-phosphosulfate reductase, adenosine phosphosulfate reductase, adenosine-5'-phosphosulfate reductase, adenylyl sulfate reductase, adenylylsulfate reductase, adenylylsulphate reductase, AdoPSO4 reductase, AMP,sulfite:flavin oxidoreductase, APR, APR1, APR2, APR3, AprA, AprB, AprBA, APS reductase, APS-reductase, APSR, AR, ATAPR2, Dde_1110, dissimilatory APS reductase, dissimilatory APS reductases, EAPR, EiAPR, FAD, FeS-enzyme adenosine-5'-phosphosulfate reductase, LMAPR, reductase, adenylylsulfate

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.99 With unknown physiological acceptors
                1.8.99.2 adenylyl-sulfate reductase

Purification

Purification on EC 1.8.99.2 - adenylyl-sulfate reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DE-52 column chromatography, macro-DEAE column chromatography, and hydroxyapatite column chromatography
-
DEAE-Biogel A chromatography, Source 15 column chromatography, and Superdex 200 column chromatography
-
DEAE-Toyopearl 650S column chromatography, Sephacryl S-200 gel filtration, and Superdex 200 gel filtration
B8PS61; B8DS74
HiTrap chelating column chromatography
-
isolated under the exclusion of oxygen
Ni2+ affinity chromatography
Ni2+-affinity column chromatography
-
partial purification by ammonium sulfate precipitation and Q-Sepharose column chromatography
F6KEW4; F6KEW3
purification of a recombinant His-tagged protein and several mutants by Ni2+ affinity chromatography
-
purification of a recombinant tagged protein by chitin affinity chromatography
-