Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.8.99.B2: tetrathionate reductase

This is an abbreviated version!
For detailed information about tetrathionate reductase, go to the full flat file.

Word Map on EC 1.8.99.B2

Reaction

tetrathionate
+
a reduced electron acceptor
= 2 thiosulfate +
an oxidized electron acceptor

Synonyms

AFE_0042, C8J_0815, CjTsdA, di-heme TsdA, tetrathionate-forming thiosulfate dehydrogenase, tetrathionate-forming TSD, thiosulfate dehydrogenase, thiosulfate:quinone reductase, TQO, TSD, TsdA, ttrA, WsTsdA

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.99 With unknown physiological acceptors
                1.8.99.B2 tetrathionate reductase

Cloned

Cloned on EC 1.8.99.B2 - tetrathionate reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene tsdA, recombinant expression of Strep-tagged enzyme in Escherichia coli strains BL21(DE3) and C43(DE3), coexpression with Strep-tagged Wolinella succinogenes TsdC
-
genes PSR1_00330 /ttrA and PSR1_003329/ttrB, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis. The putative psr1_00329-psr1_00330 operon lacks a gene encoding a membrane anchor subunit of tetrathionate reductase (TtrC), but a gene encoding a c-type cytochrome (cyt c) is adjacent to these two genes. RT-PCR enzyme expression analysis
recombinant enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
recombinant expression of wild-type and mutant Strep II-tagged enzymes
-