Any feedback?
Please rate this page
(ecexplorer.php)
(0/150)

BRENDA support

EC Explorer

EC 1.1.1.375 Details
EC number
1.1.1.375
Accepted name
L-2-hydroxycarboxylate dehydrogenase [NAD(P)+]
Reaction
a (2S)-2-hydroxycarboxylate + NAD(P)+ = a 2-oxocarboxylate + NAD(P)H + H+
Other name(s)
MdhII, lactate/malate dehydrogenase
Systematic name
(2S)-2-hydroxycarboxylate:NAD(P)+ oxidoreductase
Comment
The enzyme from the archaeon Methanocaldococcus jannaschii catalyses the reversible oxidation of (2R)-3-sulfolactate and (S)-malate to 3-sulfopyruvate and oxaloacetate, respectively (note that (2R)-3-sulfolactate has the same stereochemical configuration as (2S)-2-hydroxycarboxylates) [1]. The enzyme can use both NADH and NADPH, although activity is higher with NADPH [1-3]. The oxidation of (2R)-3-sulfolactate was observed only in the presence of NADP+ [1]. The same organism also possesses an NAD+-specific enzyme with similar activity, cf. EC 1.1.1.337, L-2-hydroxycarboxylate dehydrogenase (NAD+).
History
created 2014
EC Tree
2.5.1.12 created 1972, deleted 1976
2.5.1.13 created 1972, deleted 1976
2.5.1.14 created 1972, deleted 1976
2.5.1.37 created 1989, deleted 2004
2.5.1.40 created 1992, deleted 1999