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EC 2.8.4.3 Details
EC number
2.8.4.3
Accepted name
tRNA-2-methylthio-N6-dimethylallyladenosine synthase
Reaction
N6-(3-methylbut-2-en-1-yl)-adenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5′-deoxyadenine + electron acceptor (overall reaction);;(1a) N6-(3-methylbut-2-en-1-yl)-adenine37 in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced electron acceptor = N6-(3-methylbut-2-en-1-yl)-2-thioadenine37 in tRNA + (sulfur carrier) + L-methionine + 5′-deoxyadenine + electron acceptor ;;(1b) S-adenosyl-L-methionine + N6-(3-methylbut-2-en-1-yl)-2-thioadenine37 in tRNA = S-adenosyl-L-homocysteine + N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenine37 in tRNA
Other name(s)
MiaB, 2-methylthio-N-6-isopentenyl adenosine synthase, tRNA-i6A37 methylthiotransferase, tRNA (N6-dimethylallyladenosine37):sulfur-(sulfur carrier),S-adenosyl-L-methionine C2-methylthiotransferase
Systematic name
tRNA N6-(3-methylbut-2-en-1-yl)-adenine37:sulfur-(sulfur carrier),S-adenosyl-L-methionine C2-(methylsulfanyl)transferase
Comment
This bacterial enzyme binds two [4Fe-4S] clusters as well as the transferred sulfur [3]. The enzyme is a member of the superfamily of S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes. The sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that in vitro the reaction is a single turnover. The identity of the electron donor is not known.
History
created 2014, modified 2015