Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(-)-carvone + NADH + H+
? + NAD+
1% activity compared to cyclohexanone
-
-
?
(1S,3S)-3-methylcyclohexanol + NAD+
(rac)-3-methylcyclohexanone + NADH + H+
125% activity compared to cyclohexanol
163% activity compared to cyclohexanone
-
r
(2E)-2-methylpent-2-enal + NADPH + H+
(2E)-2-methylpent-2-en-1-ol + NADP+
41.5% of the activity with (2E)-but-2-enal, yield 95% after 12 h
-
-
?
(2E)-3,7-dimethylocta-2,6-dienal + NADPH + H+
(2E)-3,7-dimethylocta-2,6-dien-1-ol + NADP+
37.4% of the activity with (2E)-but-2-enal, yield 87% after 12 h
-
-
?
(2E)-4-hydroxynon-2-enal + NADH + H+
(2E)-non-2-ene-1,4-diol + NAD+
-
substrate of isozyme ADH4
-
-
r
(2E)-but-2-en-1-ol + NADP+
(2E)-but-2-enal + NADPH + H+
-
-
-
r
(2E)-but-2-enal + NADPH + H+
(2E)-but-2-en-1-ol + NADP+
yield 96% after 12 h
-
-
r
(2E)-dec-2-enal + NADPH + H+
(2E)-dec-2-en-1-ol + NADP+
16.7% of the activity with (2E)-but-2-enal, yield 91% after 12 h
-
-
?
(2E)-hex-2-enal + NADPH + H+
(2E)-hex-2-en-1-ol + NADP+
41.7% of the activity with (2E)-but-2-enal, yield 98% after 12 h
-
-
?
(2E)-oct-2-enal + NADPH + H+
(2E)-oct-2-en-1-ol + NADP+
30.2% of the activity with (2E)-but-2-enal, yield 69% after 12 h
plus 27% oct-2-enyl ester
-
?
(6S)-5,6-dihydro-6-methyl-4H-thieno[2,3b]thiopyran-4-one-7,7-dioxide + NADH + H+
(4S,6S)-5,6-dihydro-4-hydroxy-6-methyl-4H-thieno[2,3b]thiopyran-7,7-dioxide + NAD+
-
-
-
-
?
(E)-hex-2-en-1-ol + NAD+
(E)-hex-2-en-1-one + NADH
(R)-1-indanol + NAD+
1-indanone + NADH + H+
-
-
-
r
(R)-1-indanol + NAD+
?
62% of activity compared to (S)-1-indanol
-
-
?
(R)-1-phenyl-1-butanol + NAD+
1-phenylbutan-1-one + NADH + H+
(R)-1-phenyl-1-propanol + NAD+
1-phenyl-1-propanone + NADH + H+
-
-
-
-
r
(R)-1-phenyl-2-butanol + NAD+
1-phenylbutan-2-one + NADH + H+
-
-
-
-
r
(R)-1-phenylethanol + NAD+
acetophenone + NADH + H+
(R)-2-butanol + NAD+
2-butanone + NADH + H+
(R)-2-butanol + NAD+
butanone + NADH + H+
-
-
-
-
r
(R)-2-heptanol + NAD+
2-heptanone + NADH + H+
(R)-2-hexanol + NAD+
2-hexanone + NADH + H+
(R)-2-octanol + NAD+
2-octanone + NADH + H+
(R)-2-pentanol + NAD+
2-pentanone + NADH + H+
(R)-2-phenylpropanol + NAD+
(R)-2-phenylpropanal + NADH + H+
(R)-3-methylcyclohexanone + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
(R)-4-phenyl-2-butanol + NAD+
4-phenylbutan-2-one + NADH + H+
-
-
-
-
r
(R)-alpha-tetralol + NAD+
?
58% of activity compared to (S)-1-indanol
-
-
?
(R)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
-
-
-
r
(R,S)-2-methylbutan-1-ol + NAD+
(R,S)-2-methyl-butan-1-one + NADH + H+
(S)-(+)-1-indanol + NAD+
indanone + NADH + H+
-
-
-
-
?
(S)-1-indanol + NAD+
1-indanone + NADH + H+
(S)-1-indanol + NAD+
?
-
-
-
?
(S)-1-phenyl-1-butanol + NAD+
1-phenylbutan-1-one + NADH + H+
-
no substrate of mutant B1
-
-
r
(S)-1-phenyl-1-propanol + NAD+
1-phenyl-1-propanone + NADH + H+
-
-
-
-
r
(S)-1-phenyl-2-butanol + NAD+
1-phenylbutan-2-one + NADH + H+
-
-
-
-
r
(S)-1-phenyl-2-propanol + NAD+
phenylacetone + NADH + H+
-
9% activity compared to cyclohexanone
-
r
(S)-1-phenylethanol + NAD+
1-phenylethanone + NADH + H+
-
-
-
-
r
(S)-1-phenylethanol + NAD+
acetophenone + NADH + H+
(S)-2-butanol + NAD+
2-butanone + NADH
-
-
-
-
r
(S)-2-butanol + NAD+
2-butanone + NADH + H+
(S)-2-butanol + NAD+
butanone + NADH + H+
(S)-2-heptanol + NAD+
2-heptanone + NADH + H+
-
331% of the activity with 2-propanol
-
-
r
(S)-2-hexanol + NAD+
2-hexanone + NADH + H+
-
38% of the activity with 2-propanol
-
-
r
(S)-2-methylbutan-1-ol + NAD+
(S)-2-methyl-butanal + NADH + H+
(S)-2-octanol + NAD+
2-octanone + NADH + H+
(S)-2-pentanol + NAD+
2-pentanone + NADH + H+
(S)-2-pentanol + NAD+
?
11% of activity compared to (S)-1-indanol
-
-
?
(S)-2-phenylpropanol + NAD+
(S)-2-phenylpropanal + NADH + H+
-
156% of the activity with 2-phenylethanol
-
-
?
(S)-4-phenyl-2-butanol + NAD+
4-phenylbutan-2-one + NADH + H+
-
-
-
-
r
(S)-4-phenylbutan-2-ol + NAD+
benzylacetone + NADH + H+
-
3% activity compared to cyclohexanone
-
r
(S)-alpha-tetralol + NAD+
?
12% of activity compared to (S)-1-indanol
-
-
?
(S)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
(S)-heptan-2-ol + NAD+
2-heptanone + NADH + H+
-
-
-
r
(S)-pentan-2-ol + NAD+
2-pentanone + NADH + H+
-
-
-
r
(S)-perillylalcohol + NAD+
(S)-perillaldehyde + NADH + H+
52% activity compared to cyclohexanol
-
-
?
(Z)-hex-2-en-1-ol + NAD+
(Z)-hex-2-en-1-one + NADH
-
9.7% of the activity with ethanol
-
-
?
1,1-dichloroacetone + NADH + H+
1,1-dichloropropan-2-ol + NAD+
-
1078% of the activity with phenyl trifluoromethyl ketone
-
-
?
1,2-butanediol + NAD+
? + NADH + H+
1,2-hexanediol + NAD+
?
-
20% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1,2-pentanediol + NAD+
?
-
12% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1,2-propanediol + NAD+
hydroxyacetone + NADH
-
-
-
-
?
1,2-propanediol + NAD+
hydroxyacetone + NADH + H+
-
-
-
-
?
1,3-butanediol + NAD+
?
-
39% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1,3-propanediol + 2 NAD+ + H2O
propanedial + 2 NADH + 2 H+
-
-
-
r
1,3-propanediol + NAD+
? + NADH
Megalodesulfovibrio gigas
-
7% of the activity with ethanol
-
-
?
1,3-propanediol + NAD+
? + NADH + H+
1,4-butanediol + NAD+
? + NADH + H+
best substrate in oxidation reaction
-
-
?
1,5-pentanediol + NAD+
? + NADH + H+
18% of the activity with butan-1-ol
-
-
?
1-(3-bromophenyl)ethanol + NAD+
1-(3-bromophenyl)ethanone + NADH + H+
315% of the activity with 1-phenylethanol
-
-
?
1-(3-chlorophenyl)ethanol + NAD+
1-(3-chlorophenyl)ethanone + NADH + H+
205% of the activity with 1-phenylethanol
-
-
?
1-(4'-chlorophenyl)ethanol + NAD+
1-(4'-chlorophenyl)ethanone + NADH + H+
-
26% of the activity with (S)-(-)-1-phenylethanol
-
-
?
1-(4'-fluorophenyl)ethanol + NAD+
1-(4'-fluorophenyl)ethanone + NADH + H+
-
45% of the activity with (S)-1-phenylethanol
-
-
r
1-(4-bromophenyl)ethanol + NAD+
1-(4-bromophenyl)ethanone + NADH + H+
167% of the activity with 1-phenylethanol
-
-
?
1-(4-chlorophenyl)ethanol + NAD+
1-(4-chlorophenyl)ethanone + NADH + H+
151% of the activity with 1-phenylethanol
-
-
?
1-(4-fluorophenyl)ethanol + NAD+
1-(4-fluorophenyl)ethanone + NADH + H+
1-(4-methylphenyl)ethanol + NAD+
1-(4-methylphenyl)ethanone + NADH + H+
189% of the activity with 1-phenylethanol
-
-
?
1-(p-tolyl)-ethanol + NAD+
1-(4-methylphenyl)ethanone + NADH + H+
19% activity compared to cyclohexanol
26% activity compared to cyclohexanone
-
?
1-butanal + NADH + H+
1-butanol + NAD+
-
-
-
-
r
1-butanol + NAD+
butanal + NADH
1-butanol + NAD+
butanal + NADH + H+
1-butanol + NAD+
butyraldehyde + NADH + H+
1-chloro-5-acetylfuro[2,3-c]pyridine + NADH + H+
1-chloro-5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+
-
-
-
-
r
1-decalone + NADH + H+
decahydronaphthalen-1-ol + NAD+
85% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
1-decanol + NAD+
decanal + NADH + H+
1-dodecanol + NAD+
dodecanal + NADH
1-heptanol + NAD+
1-heptanal + NADH + H+
-
-
-
r
1-heptanol + NAD+
heptanal + NADH + H+
1-hexanal + NADH + H+
1-hexanol + NAD+
-
-
-
-
r
1-hexanol + NAD+
1-hexanal + NADH + H+
1-hexanol + NAD+
?
-
15% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
1-hydroxy-2-butanone + NADH + H+
butane-1,2-diol + NAD+
-
59% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
1-hydroxymethyl-6-methylpyrene + NAD+
1-formyl-6-methylpyrene + NADH + H+
-
-
-
r
1-hydroxymethyl-8-methylpyrene + NAD+
1-formyl-8-methylpyrene + NADH + H+
-
-
-
r
1-hydroxymethylpyrene + NAD+
1-formylpyrene + NADH + H+
-
-
-
r
1-indanol + NAD+
1-indanone + NADH + H+
1-indanone + NADH + H+
(S)-1-indanol + NAD+
1-indanone + NADH + H+
1-indanol + NAD+
-
-
-
-
r
1-nonanol + NAD+
nonanal + NADH + H+
1-octanol + NAD+
1-octanal + NADH + H+
-
6% of activity with N-benzyl-3-pyrrolidinol
-
-
?
1-octanol + NAD+
octanal + NADH
1-octanol + NAD+
octanal + NADH + H+
1-pentanol + NAD+
1-pentanal + NADH + H+
1-pentanol + NAD+
pentanal + NADH + H+
1-phenyl-1,2-ethanediol + NAD+
1-phenyl-2-propanone + NADH + H+
1% activity compared to cyclohexanol
-
-
r
1-phenyl-1,2-propandione + NADH + H+
1-phenyl-2-hydroxy-1-propanone + NAD+
-
146% of the activity with 2,2,2-trifluoroacetophenone
-
-
r
1-phenyl-1,2-propanedione + NADH
?
about 25% of the activity compared to isatin
-
-
?
1-phenyl-1,2-propanedione + NADH + H+
?
1-phenyl-1-propanol + NAD+
1-phenyl-1-propanone + NADH + H+
-
59% of the activity with (S)-(-)-1-phenylethanol
-
-
?
1-phenyl-1-propanol + NAD+
1-phenylpropan-1-one + NADH + H+
-
59% of the activity with (S)-1-phenylethanol
-
-
r
1-phenyl-1-propanol + NAD+
?
-
31% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1-phenyl-2-butanone + NADH + H+
1-phenylbutan-2-ol + NAD+
1-phenyl-2-propanol + NAD+
1-phenyl-2-propanone + NADH + H+
1-phenyl-2-propanone + NADH + H+
(S)-1-phenyl-2-propanol + NAD+
-
-
-
-
?
1-phenyl-3-butanone + NADH + H+
1-phenylbutan-3-ol + NAD+
-
353% of the activity with phenyl trifluoromethyl ketone
-
-
?
1-phenylethanol + NAD+
1-phenylethanone + NADH
1-phenylethanol + NAD+
1-phenylethanone + NADH + H+
1-phenylethanol + NAD+
acetophenone + NADH + H+
-
1% activity compared to cyclohexanone
-
?
1-phenylethanol + NADP+
1-phenylethanone + NADPH + H+
-
-
-
?
1-propanol + NAD+
propanal + NADH
-
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
1-propanol + NAD+
propanaldehyde + NADH + H+
1-propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
?
11-cis-retinal + NADH + H+
11-cis-retinol + NAD+
-
-
-
-
?
11-cis-retinol + NAD+
11-cis-retinal + NADH
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
12-hydroxylauric acid methyl ester + NAD+
12-oxolauric acid methyl ester + NADH + H+
-
product is a key intermediate for biobased polyamide 12 production
-
?
12-oxolauric acid methyl ester + NADH + H+
12-hydroxylauric acid methyl ester + NAD+
-
-
-
?
13-cis-retinal + NADH + H+
13-cis-retinol + NAD+
-
-
-
-
?
13-cis-retinol + NAD+
13-cis-retinal + NADH
16-hydroxyhexadecanoate + NAD+
16-oxohexadecanoic acid + NADH
-
-
-
-
?
17beta-hydroxyetiocholan-3-one + NAD+
ethiocholan-3,17-dione + NADH
-
-
-
-
?
2',3',4',5',6'-pentafluoroacetophenone + NADH + H+
1-(2,3,4,5,6-pentafluorophenyl)ethanol + NAD+
45% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
2,2'-dichlorobenzil + NADH + H+
1,2-bis(2-chlorophenyl)-2-hydroxyethanone + NAD+
-
-
-
?
2,2,2-trichloroethanol + NAD+
trichloroacetaldehyde + NADH + H+
-
-
-
-
?
2,2,2-trifluoro-1-phenylethanone + propan-2-ol
(1R)-2,2,2-trifluoro-1-phenylethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 99% enantiomeric excess
-
?
2,2,2-trifluoroacetophenone + NADH
2,2,2-trifluoro-1-phenylethanol + NAD+
about 35% of the activity compared to isatin
-
-
?
2,2,2-trifluoroacetophenone + NADH + H+
(R)-alpha-(trifluoromethyl)benzyl alcohol + NAD+
2,2,2-trifluoroacetophenone + NADH + H+
2,2,2-trifluoro-1-phenylethanol + NAD+
2,2,2-trifluoroacetophenone + NADPH + H+
(R)-2,2,2-trifluoro-1-phenylethanol + NADP+
180% of the activity with acetoin
-
-
r
2,2,2-trifluoroethanol + NAD+
2,2,2-trifluoroethanone + NADH + H+
-
-
-
?
2,2-dichloroacetophenone + NADH + H+
2,2-dichloro-1-phenylethanol + NAD+
2,2-difluoro-1-phenylethanone + propan-2-ol
(1R)-2,2-difluoro-1-phenylethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 94% enantiomeric excess
-
?
2,3'-dichloroacetophenone + NADH + H+
2-chloro-1-(3-chlorophenyl)ethanol + NAD+
2,3,4,5,6-pentafluorobenzyl alcohol + NAD+
2,3,4,5,6-pentafluorobenzaldehyde + NADH + H+
-
-
-
?
2,3-butanediol + H2O
?
-
-
-
?
2,3-butanediol + NAD+
3-hydroxy-2-butanone + NADH + H+
-
-
-
-
r
2,3-butanediol + NAD+
?
-
83% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
2,3-butanediol + NAD+
acetoin + NADH + H+
2,3-butanediol + NADP+
3-hydroxy-2-butanone + NADPH + H+
-
-
-
-
?
2,3-butanediol + NADP+
acetoin + NADPH + H+
2,3-butanedione + NADH + H+
?
-
activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
2,3-pentanedione + NADH + H+
? + NAD+
-
5.5% of the activity with acetaldehyde
-
-
?
2-(3,5-dimethylphenyl)propanal + NADH + H+
(S)-2-(3,5-dimethylphenyl)propanol + NAD+
2-(3-benzoylphenyl)propanal + NADH + H+
(S)-2-(3-benzoylphenyl)propanol + NAD+
2-(3-fluorobiphenyl-4-yl)propanal + NADH + H+
(S)-2-(3-(fluoro)biphenyl-4-yl)propanol + NAD+
2-(3-phenoxyphenyl)propanal + NADH + H+
(S)-2-((3-phenoxy)phenyl)propanol + NAD+
2-(4-isobutylphenyl)propanal + NADH + H+
(S)-2-(4-isobutylphenyl)propanol + NAD+
2-(4-trifluoromethylphenyl)propanal + NADH + H+
(S)-2-(4-trifluoromethyl)phenylpropanol + NAD+
18 h, 55% yield, 98% enantiomeric excess
-
-
?
2-(6-methoxynaphthalen-2-yl)propanal + NADH + H+
(S)-2-(6-methoxynaphthalen-2-yl)propan-1-ol + NAD+
18 h, 96% yield, 98% enantiomeric excess
-
-
?
2-(naphthalen-1-yl)propanal + NADH + H+
(S)-2-(naphthalen-1-yl)propanol + NAD+
18 h, 90% yield, 80% enantiomeric excess
-
-
?
2-(naphthalen-2-yl)propanal + NADH + H+
(S)-2-(naphthalen-2-yl)propanol + NAD+
18 h, 57% yield, 94% enantiomeric excess
-
-
?
2-acetylcyclohexanone + NADH + H+
2-acetylcyclohexanol + NAD+
-
-
-
-
r
2-acetylcyclopentanone + NADH + H+
2-acetylcyclopentanol + NAD+
-
-
-
-
r
2-acetylfuran + NADH + H+
? + NAD+
-
-
-
-
r
2-acetylpyridine + NADH + H+
(R)-1-(2-pyridyl)ethanol + NAD+
-
-
-
-
r
2-acetylpyrrole + NADH + H+
1-(1H-pyrrol-2-yl)ethanol + NAD+
-
70% of activity with N-benzyl-3-pyrrolidinone
-
-
?
2-acetylpyrrole + NADH + H+
? + NAD+
-
-
-
-
r
2-acetylthiazole + NADH + H+
? + NAD+
-
-
-
-
r
2-acetylthiophene + NADH + H+
? + NAD+
-
-
-
-
r
2-aminoethanol + NAD+
?
-
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
2-butanone + NADH + H+
2-butanol + NAD+
2-butanone + NADH + H+
butan-2-ol + NAD+
-
2.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
2-butanone + NADPH + H+
butan-2-ol + NADP+
12.1% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-butene-1-ol + NAD+
? + NADH
2-chloro-1-(2,4-dichlorophenyl)ethan-1-one + NADH + H+
(1R)-2-chloro-1-(2,4-dichlorophenyl)ethan-1-ol + NAD+
AE010289.1
production of with (1R)-2-chloro-1-(2,4-dichlorophenyl)ethan-1-ol 99% enantiomeric excess
-
-
?
2-chloro-1-(2,4-difluorophenyl)ethan-1-one + NADH + H+
(1R)-2-chloro-1-(2,4-fluorophenyl)ethan-1-ol + NAD+
AE010289.1
production of (1R)-2-chloro-1-(2,4-fluorophenyl)ethan-1-ol with 99% enantiomeric excess
-
-
?
2-chloro-1-(4-fluorophenyl)ethan-1-one + NADH + H+
(1R)-2-chloro-1-(4-fluorophenyl)ethan-1-ol + NAD+
AE010289.1
production of (1R)-2-chloro-1-(4-fluorophenyl)ethan-1-ol with 98% enantiomeric excess
-
-
?
2-chloroacetophenone + NADH + H+
1-(2-chlorophenyl)ethanol + NAD+
-
low activity
-
-
r
2-chlorocyclohexanone + NADH + H+
? + NAD+
3% activity compared to cyclohexanone
-
-
?
2-decalone + NADH + H+
? + NAD+
28% activity compared to cyclohexanone
-
-
?
2-decanol + NAD+
2-decanone + NADH + H+
weak activity
-
-
r
2-decanone + NADH
(S)-2-decanol + NAD+ + H+
-
92% enantiomeric excess
-
?
2-dehydro-3-deoxy-D-gluconate + NADH + H+
4-deoxy-L-erythro-5-hexoseulose + NAD+
-
-
-
r
2-deoxy-D-ribose + NAD+
? + NADH
-
-
-
-
?
2-ethoxyethanol + NAD+
2-ethoxyacetaldehyde + NADH + H+
-
-
-
r
2-ethylhexan-1-ol + NAD+
2-ethylhexanal + NADH
Sporotrichum pulverulentum
-
weak activity
-
-
?
2-fluoro-1-(4-chlorophenyl)ethanone + propan-2-ol
(1R)-2-fluoro-1-(4-chlorophenyl)ethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 98% enantiomeric excess
-
?
2-fluoro-1-(4-fluorophenyl)ethanone + propan-2-ol
(1R)-2-fluoro-1-(4-fluorophenyl)ethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 97% enantiomeric excess
-
?
2-fluoro-1-(4-iodophenyl)ethanone + propan-2-ol
(1R)-2-fluoro-1-(4-iodophenyl)ethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 99% enantiomeric excess
-
?
2-fluoro-1-phenylethanone + propan-2-ol
(1R)-2-fluoro-1-phenylethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 99% enantiomeric excess
-
?
2-heptanol + NAD+
2-heptanone + NADH + H+
2-heptanol + NAD+
heptan-2-one + NADH + H+
62% of the activity with 1-phenylethanol
-
-
?
2-heptanone + NADH
(S)-2-heptanol + NAD+ + H+
-
79% enantiomeric excess
-
?
2-heptanone + NADH + H+
(R)-2-heptanol + NAD+
-
229% of the activity with phenyl trifluoromethyl ketone
99% enantiomeric excess
-
?
2-heptanone + NADPH + H+
heptan-2-ol + NADP+
6.8% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-hexanol + NAD+
2-hexanone + NADH + H+
2-hexanone + NADH
(S)-2-hexanol + NAD+ + H+
-
37% enantiomeric excess
-
?
2-hexanone + NADH + H+
2-hexanol + NAD+
-
-
-
-
r
2-hexanone + NADH + H+
?
-
activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
2-hexanone + NADH + H+
hexan-2-ol + NAD+
-
50.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
2-hexanone + NADPH + H+
hexan-2-ol + NADP+
5.9% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-hydroxyacetophenone + NADH + H+
(S)-1-phenyl-1,2-ethanediol + NAD+
2.4% of the activity with NADPH
-
-
?
2-hydroxyacetophenone + NADPH + H+
(S)-1-phenyl-1,2-ethanediol + NADP+
-
-
-
?
2-hydroxymethylpyrene + NAD+
2-formylpyrene + NADH + H+
-
-
-
r
2-mercaptoethanol + NAD+
?
2-methoxybenzaldehyde + NADH + H+
2-methoxybenzylalcohol + NAD+
-
13% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
2-methoxybenzyl alcohol + NAD+
2-methoxybenzaldehyde + NADH + H+
-
25% of the activity with (S)-(-)-1-phenylethanol
-
-
?
2-methoxyethanol + NAD+
2-methoxyacetaldehyde + NADH + H+
-
-
-
r
2-methyl-2,4-pentanediol + NAD+
?
1% activity compared to cyclohexanol
-
-
?
2-methylbutan-1-ol + NAD+
? + NADH
-
-
-
-
?
2-methylbutyraldehyde + NADH + H+
2-methylbutanol + NAD+
-
1.4% of the activity with acetaldehyde
-
-
?
2-methylcyclohexanol + NAD+
(+)-2-methylcyclohexanone + NADH + H+
-
-
-
-
r
2-methylcyclohexanone + NADH + H+
2-methylcyclohexanol + NAD+
13% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
2-methylcyclohexanone + NADH + H+
? + NAD+
46% activity compared to cyclohexanone
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH + H+
2-methylpropionaldehyde + NADH + H+
2-methylpropanal + NAD+
-
3.3% of the activity with acetaldehyde
-
-
?
2-nitrobenzaldehyde + NADH + H+
(2-nitrophenyl)methanol + NAD+
-
33% of activity with N-benzyl-3-pyrrolidinone
-
-
?
2-nitrobenzaldehyde + NADH + H+
2-nitrobenzyl alcohol + NAD+
-
low activity
-
-
r
2-nonanol + NAD+
2-nonanone + NADH + H+
weak activity
-
-
r
2-nonanone + NADH
(S)-2-nonanol + NAD+ + H+
-
95% enantiomeric excess
-
?
2-octanol + NAD+
2-octanone + NADH + H+
2-octanone + NADH
(S)-2-octanol + NAD+ + H+
-
92% enantiomeric excess
-
?
2-octanone + NADH + H+
2-octanol + NAD+
-
-
-
-
r
2-octanone + NADPH + H+
octan-2-ol + NADP+
11.9% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-oxo-3-methylpentane + NADH
3-methylpentan-2-ol + NAD+
2-oxo-3-phenylpropane + NADH
2-hydroxy-3-phenylpropane + NAD+
2-oxo-4-methylpentane + NADH
4-methylpentan-2-ol + NAD+
-
-
-
-
?
2-oxo-butyric acid + NADH + H+
2-hydroxybutyric acid + NAD+
-
15% of activity with N-benzyl-3-pyrrolidinone
-
-
?
2-oxohexane + NADH
2-hydroxyhexane + NAD+
-
-
-
-
?
2-oxopentane + NADH
2-pentanol + NAD+
-
-
-
-
?
2-oxopentanoate + NADH + H+
2-hydroxypentanoate + NAD+
-
low activity
-
-
r
2-oxopropanal + NADH + H+
? + NAD+
328% of the activity with acetoin
-
-
r
2-pentanol + NAD+
2-pentanone + NADH + H+
2-pentanone + NADH + H+
(S)-2-pentanol + NAD+
-
60% enantiomeric excess
-
?
2-pentanone + NADH + H+
2-pentanol + NAD+
2-pentanone + NADH + H+
pentan-2-ol + NAD+
-
-
-
r
2-pentanone + NADPH + H+
pentan-2-ol + NADP+
11.9% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-phenylcyclohexanone + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
2-phenylethanol + NAD+
2-phenylethanone + NADH + H+
57% activity compared to cyclohexanol
-
-
r
2-phenylethanol + NAD+
phenylacetaldehyde + NADH + H+
-
-
-
-
r
2-phenylpropanal + NADH + H+
(S)-2-phenylpropanol + NAD+
18 h, 74% yield, 98% enantiomeric excess
-
-
?
2-phenylpropionaldehyde + NADH + H+
2-phenylpropanol + NAD+
-
-
-
-
r
2-propanol + NAD(P)+
acetone + NAD(P)H
2-propanol + NAD+
2-propanone + NADH
2-propanol + NAD+
2-propanone + NADH + H+
2-propanol + NAD+
aceton + NADH + H+
-
-
irreversible, no measurable activity with acetone
-
ir
2-propanol + NAD+
acetone + NADH
-
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
2-propanol + NAD+
propanone + NADH + H+
-
-
-
-
r
2-[3-(2-phenyl-1,3-dioxolan-2-yl)phenyl]propanal + NADH + H+
(S)-2-(3-(2-phenyl-1,3-dioxolan-2-yl)phenyl)propanol + NAD+
18 h, 95% yield, 61% enantiomeric excess
-
-
?
3',4'-dimethoxyphenylacetone + NADH + H+
1-(3,4-dimethoxyphenyl)propan-2-ol + NAD+
-
24% of the activity with phenyl trifluoromethyl ketone
-
-
?
3'-bromoacetophenone + NADH + H+
1-(3-bromophenyl)ethanol + NAD+
-
151% of the activity with phenyl trifluoromethyl ketone
-
-
?
3'-chloroacetophenone + NADH + H+
1-(3-chlorophenyl)ethanol + NAD+
-
70% of the activity with phenyl trifluoromethyl ketone
-
-
?
3'-methoxyacetophenone + NADH + H+
1-(3-methoxyphenyl)ethanol + NAD+
-
51% of the activity with phenyl trifluoromethyl ketone
-
-
?
3,3,5-trimethylcyclohexanone + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
3,4-dihydro-retinol + NAD+
3,4-dihydro-retinal
-
-
-
-
r
3,4-dimethylbenzyl alcohol + NAD+
3,4-dimethylbenzaldehyde + NADH + H+
3,4-hexanedione + NADH + H+
?
-
77% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
3,4-methylenedioxyphenyl acetone + NADH
(S)-(3,4-methylenedioxyphenyl)-2-propanol + NAD+ + H+
-
-
-
-
?
3,5-dimethylcyclohexanol + NAD+
3,5-dimethylcyclohexanone + NADH + H+
1% activity compared to cyclohexanol
-
-
r
3-acetylpyridine + NADH + H+
(R)-1-(3-pyridyl)ethanol + NAD+
-
-
-
-
r
3-acetylpyridine + NADH + H+
1-pyridin-3-ylethanol + NAD+
-
7.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
3-aminobenzyl alcohol + NAD+
3-aminobenzaldehyde + NADH + H+
8% activity compared to cyclohexanol
-
-
r
3-bromobenzyl alcohol + NAD+
3-bromobenzaldehyde + NADH + H+
-
-
-
-
?
3-bromobenzylalcohol + NAD+
3-bromobenzaldehyde + NADH + H+
-
-
-
-
?
3-butene-1-ol + NAD+
? + NADH
-
-
-
-
?
3-chloro-2-butanone + NADH + H+
3-chlorobutan-2-ol + NAD+
-
151% of the activity with phenyl trifluoromethyl ketone
-
-
?
3-chlorobenzaldehyde + NADH + H+
3-chlorobenzyl alcohol + NAD+
-
-
-
-
r
3-heptanol + NAD+
3-heptanone + NADH + H+
-
93% of the activity with 2-propanol
-
-
r
3-hydroxy-2-butanone + NADH + H+
2,3-butanediol + NAD+
-
-
-
-
r
3-hydroxy-2-butanone + NADPH + H+
2,3-butanediol + NADP+
-
-
-
-
r
3-methoxy-1-phenylpropan-1-one + NADH
3-methoxy-1-phenylpropan-1-ol + NAD+
-
-
-
-
?
3-methoxybenzaldehyde + NADH + H+
3-methoxybenzyl alcohol + NAD+
-
-
-
-
r
3-methoxybenzaldehyde + NADH + H+
3-methoxybenzylalcohol + NAD+
-
14% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
3-methoxybenzyl alcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
3-methoxybenzylalcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
-
-
-
-
?
3-methyl-1-butanol + NAD+
? + NADH
-
-
-
-
?
3-methyl-2-cyclohexenone + NADH + H+
? + NAD+
1% activity compared to cyclohexanone
-
-
?
3-methylbutan-1-ol + NAD+
3-methyl-butan-1-one + NADH + H+
3-methylbutan-2-ol + NAD+
3-methylbutan-2-one + NADH
-
R-(-)-3-methylbutan-2-ol and S-(+)-3-methylbutan-2-ol
-
-
?
3-methylbutan-2-one + NADH
3-methylbutan-2-ol + NAD+
-
-
-
-
?
3-methylbutan-2-one + NADH + H+
3-methyl-2-butanol + NAD+
-
-
-
-
r
3-methylbutan-2-one + NADH + H+
3-methylbutan-2-ol + NAD+
-
-
-
-
?
3-methylbutanal + NAD+
? + NADH + H+
3-methylbutanal + NADPH + H+
3-methylbutanol + NADP+
low activity, reaction of EC 1.1.1.2
-
-
ir
3-methylbutanol + NAD+
3-methylbutanone + NADH + H+
133% activity compared to cyclohexanol
-
-
r
3-methylbutanol + NAD+
? + NADH
3-methylbutyraldehyde + NADH + H+
3-methylbutanol + NAD+
-
2.9% of the activity with acetaldehyde
-
-
?
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH
-
-
-
-
r
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH + H+
14% of activity compared to (S)-1-indanol
-
-
?
3-methylcyclohexanol + NAD+
?
10% of the activity compared to isoborneol
-
-
?
3-methylcyclohexanone + NADH
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH + H+
3-methylcyclohexanol + NAD+
33% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
3-methylphenylethyl alcohol + NAD+
?
64% activity compared to cyclohexanol
-
-
?
3-nitroacetophenone + NADH + H+
1-(3-nitrophenyl)ethanol + NAD+
-
-
-
-
r
3-nitrobenzaldehyde + NADH + H+
3-nitrobenzyl alcohol + NAD+
3-oxo-5beta-androstan-17beta-ol + NADH
3beta,17beta-dihydroxy-5beta-androstane + NAD+
3-oxoheptane + NADH
3-hydroxyheptane + NAD+
-
-
-
-
?
3-pentanol + NAD+
3-pentanone + NADH + H+
3-pentanone + NADH
pentan-3-ol + NAD+
-
-
-
r
3-pentanone + NADPH + H+
pentan-3-ol + NADP+
7.1% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
3-penten-2-one + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
3-phenyl-1-propanol + NAD+
3-phenyl-1-propanone + NADH
-
-
-
-
?
3-phenyl-2-propen-1-ol + NAD+
? + NADH
-
-
-
-
?
3-phenylpropanol + NAD+
3-phenylpropanal + NADH + H+
-
135% of the activity with 2-phenylethanol
-
-
?
3-phenylpropionaldehyde + NADH
3-phenylpropan-1-ol + NAD+
-
1218% of the activity with phenyl trifluoromethyl ketone
-
-
?
3-phenylpropionaldehyde + NADH + H+
3-phenylpropanol + NAD+
-
6.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
3-pyridylcarbinol + NAD+
pyridine-3-carbaldehyde + NADH
-
-
-
-
?
3beta,12alpha-dihydroxy-5beta-cholanoic acid + NAD+
? + NADH
-
-
-
-
?
3beta,17beta-dihydroxy-5beta-androstane + NAD+
5beta-androstan-3,17dione + NADH
-
-
-
-
?
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoic acid + NAD+
? + NADH
-
-
-
-
?
3beta,7alpha-dihydroxy-5beta-cholanoic acid + NAD+
? + NADH
-
-
-
-
?
3beta-7alpha-dihydroxy-5beta-cholanoate + NAD+
17-hydroxy-3-oxo-5beta-cholanoate + NADH + H+
-
-
-
-
r
3beta-hxdroxy-5alpha-cholanoate + NAD+
3-oxo-5alpha-cholanoate + NADH
-
-
-
-
?
3beta-hydroxy-5beta-androstan-17-one + NAD+
5beta-androstan-3,17-dione + NADH
3beta-hydroxy-5beta-cholanoate + NAD+
3-oxo-5beta-cholanoate + NADH
-
-
-
-
?
4'-bromoacetophenone + NADH + H+
1-(4-bromophenyl)ethanol + NAD+
-
77% of the activity with phenyl trifluoromethyl ketone
-
-
?
4'-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
-
60% of the activity with phenyl trifluoromethyl ketone
-
-
?
4'-chlorobutyrophenone + NADH + H+
1-(4-chlorophenyl)butan-1-ol + NAD+
-
10% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
4-acetylpyridine + NADH + H+
(R)-1-(4-pyridyl)ethanol + NAD+
-
-
-
-
r
4-acetylpyridine + NADH + H+
1-pyridin-4-ylethanol + NAD+
-
64.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
4-amino-1-butanol + NAD+
to gamma-butyrolactam + NADH + H+
95% conversion
-
-
?
4-bromoacetophenone + NADH + H+
1-(4-bromophenyl)ethanol + NAD+
4-bromobenzyl alcohol + NAD+
4-bromobenzaldehyde + NADH + H+
-
-
-
-
?
4-bromobenzylalcohol + NAD+
4-bromobenzaldehyde + NADH + H+
-
-
-
-
?
4-carboxybenzaldehyde + NADH + H+
4-carboxybenzyl alcohol + NAD+
-
-
-
-
?
4-carboxybenzaldehyde + NADH + H+
4-carboxybenzylalcohol + NAD+
-
-
-
-
?
4-chloroacetophenone + NADH
1-(4-chlorophenyl)ethanol + NAD+
4-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
4-chlorobenzaldehyde + NADH + H+
4-chlorobenzyl alcohol + NAD+
-
-
-
-
r
4-deoxy-L-erythro-5-hexoseulose + NAD+
2-dehydro-3-deoxy-D-gluconate + NADH + H+
4-ethylcyclohexanol + NAD+
4-ethylcyclohexanone + NADH + H+
60% activity compared to cyclohexanol
-
-
r
4-ethylcyclohexanone + NADH + H+
? + NAD+
22% activity compared to cyclohexanone
-
-
?
4-fluoroacetophenone + NADH + H+
1-(4-fluorophenyl)ethanol + NAD+
4-hydroxy-2-butanone + NADH + H+
2,4-dihydroxybutane + NAD+
-
26% of the activity with phenyl trifluoromethyl ketone
-
-
?
4-hydroxy-retinol + NAD+
4-oxo-retinal + NADH
-
-
-
-
r
4-hydroxymethylpyrene + NAD+
4-formylpyrene + NADH + H+
-
-
-
r
4-methoxy-1-naphthaldehyde + NAD+
4-methoxy-1-naphthol + NADH + H+
-
fluorogenic substrate of class I and II isozymes
-
-
?
4-methoxy-1-naphthaldehyde + NAD+
4-methoxy-1-naphthyl alcohol + NADH + H+
-
substrate for class I ADH
-
-
?
4-methoxy-1-naphthaldehyde + NADH + H+
4-methoxynaphthalene-1-carbaldehyde + NAD+
-
substrate for class I ADH
-
-
r
4-methoxybenzaldehyde + NADH + H+
4-methoxybenzyl alcohol + NAD+
-
51% of the activity with butan-2-ol
-
?
4-methoxybenzaldehyde + NADH + H+
4-methoxybenzylalcohol + NAD+
-
13% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
4-methoxybenzylalcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
-
-
-
r
4-methoxyphenylacetone + NADH
(2S)-1-(4-methoxyphenyl)propan-2-ol + NAD+ + H+
-
-
-
?
4-methyl-1-pentanol + NAD+
4-methyl-1-pentanal + NADH
4-methylcyclohexanol + NAD+
4-methylcyclohexanone + NADH + H+
56% activity compared to cyclohexanol
-
-
r
4-methylcyclohexanone + NADH + H+
4-methylcyclohexanol + NAD+
60% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
4-methylcyclohexanone + NADH + H+
? + NAD+
25% activity compared to cyclohexanone
-
-
?
4-methylpentan-2-one + NADH + H+
4-methyl-2-pentanol + NAD+
-
-
-
-
r
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzyl alcohol + NAD+
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzylalcohol + NAD+
-
-
-
-
?
4-nitrobenzyl alcohol + NAD+
4-nitrobenzaldehyde + NADH + H+
94% activity compared to benzyl alcohol
-
-
?
4-nitrosodimethylaniline + NAD+
? + NADH + H+
-
photometric assay substrate
-
-
?
4-nitrotetrazolium violet + NADH + H+
a formazan + NAD+
4-phenylbutan-2-one + NADH
4-phenylbutan-2-ol + NAD+
-
-
-
-
?
5-acetyl-7-chlorofuro[2,3-c]pyridine + NADH + H+
5-(1-hydroxyethyl)-7-chlorofuro[2,3-c]pyridine + NAD+
-
-
-
-
r
5-acetylfuro[2,3-c]pyridine + NADH + H+
5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+
-
-
-
-
r
5-amino-1-pentanol + NAD+
delta-valerolactam + NADH + H+
38% conversion
-
-
?
5-hydroxymethylfurfural + NADH + H+
(furan-2,5-diyl)dimethanol + NAD+
5-hydroxymethylfurfural + NADH + H+
5-hydroxymethylfurfuryl alcohol + NAD+
low activity
-
-
ir
5alpha-androstan-17beta-ol-3-one + NADH + H+
3beta,17beta-dihydroxy-5alpha-androstan + NAD+
5alpha-pregnan-3beta-ol-20-one + NAD+
5alpha-pregnan-3,20-dione + NADH
-
low activity
-
-
?
5beta-androstan-17beta-ol-3-one + NAD+
5beta-androstan-3,17-dione + NADH
5beta-androstan-3beta-ol-17-one + NAD+
5beta-androstan-3,17-dione + NADH
5beta-cholanic acid-3-one + NADH
5beta-cholanic acid-3-ol + NAD+
-
low activity
-
-
?
5beta-cholanic acid-3-one + NADH + H+
5beta-cholanic acid-3-ol + NAD+
-
-
-
-
?
5beta-pregnan-21-ol-3,20-dione hemisuccinate + NAD+
5beta-pregnan-3,20,21-trione hemisuccinate + NADH + H+
-
-
-
-
?
5beta-pregnan-3,20-dione + NADH
?
-
-
-
-
?
5beta-pregnan-3beta-ol-20-one + NAD+
5beta-pregnan-3,20-dione + NADH
-
-
-
-
?
6-amino-1-hexanol + NAD+
epsilon-caprolactam + NADH + H+
14% conversion
-
-
?
6-benzyloxy-3,5-dioxo-hexanoic acid ethyl ester + NADH + H+
(3R,5S)-6-benzyloxy-3,5-dihydroxy-hexanoic acid ethyl ester + NAD+
-
-
-
-
?
6-methoxy-2-naphthaldehyde + NADH + H+
(6-methoxynaphthalen-2-yl)methanol + NAD+
7-cis-retinol + NAD+
7-cis-retinal + NADH
9-cis-retinol + NAD+
9-cis-retinal + NADH
a primary alcohol + NAD+
an aldehyde + NADH + H+
-
ADH3 is involved in multiple cellular pathways, as diverse as formaldehyde detoxification, retinoid metabolism and NO homeostasis, ADH3 is considered to play only a minor role in hepatic alcohol metabolism because ethanol concentrations rarely exceed 50 mM
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
acetaldehyde + NADH + H+
ethanol + NAD+ + H+
acetaldehyde + NADPH + H+
ethanol + NADP+
acetoin + NAD+
diacetyl + NADH
18% of the activity with 2,3-butanediol
-
-
?
acetoin + NADH + H+
2,3-butanediol + NAD+
-
-
-
r
acetoin + NADH + H+
3-hydroxy-2-butanol + NAD+
-
-
-
-
r
acetoin + NADH + H+
butan-2,3-diol + NAD+
-
-
-
?
acetone + NADH
isopropanol + NAD+
acetone + NADH + H+
2-propanol + NAD+
-
-
-
-
?
acetone + NADH + H+
propan-2-ol + NAD+
acetone + NADPH + H+
propan-2-ol + NADP+
14.7% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
acetophenone + NADH
(R)-alpha-phenyl ethanol + NAD+
-
the enzyme exhibits high stereoselectivity in the desymmetrization of the prochiral ketone acetophenone, producing optically pure (R)-alpha-phenyl ethanol at high conversion
-
-
?
acetophenone + NADH + H+
(R)-1-phenylethanol + NAD+
-
6% of the activity with phenyl trifluoromethyl ketone, in the reverse reaction 87% of the activity with 2-propanol
99% enantiomeric excess
-
?
acetophenone + NADH + H+
(S)-(-)-1-phenylethanol + NAD+
-
more than 99% enantiomeric excess
-
-
r
acetophenone + NADH + H+
(S)-1-phenylethanol + NAD+
-
-
99% enantiomeric excess
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
acetophenone + NADPH + H+
1-phenylethanol + NADP+
5.1% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
acetylacetone + 2 NADH + 2 H+
2,4-pentanediol + 2 NAD+
-
-
-
-
r
acetylacetone + NADH + H+
4-hydroxypentan-2-one + NAD+
1% activity compared to cyclohexanone
1% activity compared to cyclohexanone
-
r
acetylacetone + NADH + H+
?
-
63% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
acetylpyrazine + NADH + H+
(R)-1-(pyrazyl)ethanol + NAD+
-
-
-
-
r
alcohol + NAD+
aldehyde or ketone + NADH
all-trans retinal + NADH + H+
all-trans retinol + NAD+
26.8% of the activity with butan-1-ol
-
-
?
all-trans-retinal + NADH + H+
all-trans-retinol + NAD+
-
-
-
-
?
all-trans-retinol + NAD+
all-trans-retinal + NADH
allyl alcohol + NAD+
acrolein + NADH
allyl alcohol + NAD+
prop-2-en-1-al + NADH
allylalcohol + NAD+
prop-2-enal + NADH + H+
-
-
-
?
alpha-ethyl benzoylformate + NADH + H+
ethyl (R)-(-)-mandelate + NAD+
-
-
95% enantiomeric excess
-
r
alpha-methyl benzoylformate + NADH + H+
methyl (R)-mandelate + NAD+
-
-
92% enantiomeric excess
-
r
alpha-tetralone + NADH + H+
(S)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
alpha-tetralol + NAD+
-
-
-
-
r
anisaldehyde + NADH + H+
anisic alcohol + NAD+
-
-
-
-
?
benzacetaldehyde + NADPH + H+
benzol + NADP+
low activity, reaction of EC 1.1.1.2
-
-
ir
benzaldehyde + NADH
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
107% of the activity with (2E)-but-2-enal, yield 99% after 12 h
-
-
r
benzil + NADH + H+
(R)-benzoin + NAD+
62% of the activity compared to 1-phenyl-1,2-propanedione. The enzyme catalyses the asymmetric reduction of benzil to (R)-benzoin with both excellent conversion (98%) and optical purity (98%) by way of an efficient in situ NADH-recycling system involving a second thermophilic ADH
-
-
?
benzoin + NADH + H+
1,2-diphenylethane-1,2-diol
-
13% of activity with N-benzyl-3-pyrrolidinone
-
-
?
benzoylformic acid + NADPH + H+
mandelate + NADP+
2.4% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
benzyl alcohol + acycloNAD+
benzaldehyde + acycloNADH + H+
-
acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
-
-
r
benzylacetone + NADH + H+
4-phenylbutan-2-ol + NAD+
benzylalcohol + NAD+
benzaldehyde + NADH + H+
benzyloxycarbonyl-3-pyrrolidinone + NADH + H+
(S)-benzyloxycarbonyl-3-pyrrolidinol + NAD+
-
production with 99.6% enantiomeric excess
-
-
?
butan-1-ol + acycloNAD+
butanal + acycloNADH + H+
-
acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety
-
-
?
butan-1-ol + NAD+
butanal + NADH + H+
butan-2,3-diol + NAD+
acetoin + NADH + H+
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
butan-2-ol + NAD+
butanone + NADH + H+
-
83% of the activity with butan-2-ol
-
?
butan-2-one + NADH
butan-2-ol + NAD+
butanal + NADH
1-butanol + NAD+ + H+
-
% of the activity with phenyl trifluoromethyl ketone
-
-
ir
butanal + NADH
n-butanol + NAD+
butanal + NADH + H+
butan-1-ol + NAD+
-
-
-
r
butanol + NAD+
1-butanal + NADH + H+
about 15% of the activity with ethanol or 1-propanol
-
-
?
butanol + NAD+
butanal + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
butanol + NAD+
butyraldehyde + NADH + H+
butanol + NAD+
n-butanal + NADH
-
-
-
?
butyraldehyde + NAD+
n-butanol + NADH + H+
-
-
-
r
butyraldehyde + NADH
n-butanol + NAD+
butyraldehyde + NADH + H+
butanol + NAD+
-
56% of the activity with acetaldehyde
-
-
?
butyraldehyde + NADH + H+
n-butanol + NAD+
butyraldehyde + NADPH + H+
butanol + NADP+
-
22% of the activity with NADH
-
-
?
capronaldehyde + NADH
1-hexanol + NAD+
chloroacetone + NADH + H+
1-chloro-2-propanol + NAD+
-
-
-
-
r
chloroacetone + NADH + H+
1-chloropropan-2-ol + NAD+
choline + NAD+
? + NADH + H+
3% activity compared to benzyl alcohol
-
-
?
cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH + H+
-
-
-
-
r
cinnamyl alcohol + NAD+
cinnamyl aldehyde + NADH + H+
-
-
-
?
cis-4-methylcyclohexanol + NAD+
4-methylcyclohexanone + NADH
-
-
-
-
?
cis-decahydro-1-naphthol + NAD+
?
11% of activity compared to (S)-1-indanol
-
-
?
crotyl alcohol + NAD+
crotonaldehyde + NADH
cycloheptanol + NAD+
cycloheptanone + NADH + H+
cycloheptanone + NADH
cycloheptanol + NAD+
cyclohexanol + NAD+
cyclohexanone + NADH
cyclohexanol + NAD+
cyclohexanone + NADH + H+
cyclohexanone + NADH
cyclohexanol + NAD+
cyclohexanone + NADH + H+
?
-
activity is 2.1fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
cyclohexanone + NADH + H+
cyclohexanol + NAD+
cyclooctanone + NADH
cyclooctanol + NAD+
-
-
-
-
?
cyclopentanol + NAD+
? + NADH
cyclopentanol + NAD+
cyclopentanone + NADH + H+
cyclopentanone + NADH
cyclopentanol + NAD+
-
-
-
-
?
cyclopentanone + NADH + H+
? + NAD+
1% activity compared to cyclohexanone
-
-
?
cyclopetanone + NADH + H+
cyclopentanol + NAD+
-
-
-
-
?
D-allonate + NADH + H+
?
10% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-altronate + NADH + H+
?
8% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-arabinose + NADH + H+
D-arabitol + NAD+
66% of the activity with 2,3-butanediol
-
-
?
D-arabitol + NADH + H+
D-lyxose + D-arabinose + NAD+
-
-
-
?
D-erythrose + NAD+
?
-
-
-
?
D-glucitol + NAD+
? + NADH
D-gluconate + NADH + H+
?
79% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-glyceraldehyde + NAD+
?
-
-
-
?
D-mannonate + NADH + H+
?
37% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-ribonate + NADH + H+
?
0.3% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-sorbitol + NADH + H+
L-gulose + NAD+
0.01% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-talonate + NADH + H+
?
3% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-threose + NAD+
?
-
-
-
?
D-xylonate + NADH + H+
?
1% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
decahydro-2-naphthol + NAD+
?
37% activity compared to cyclohexanol
-
-
?
decan-1-ol + NAD+
decanal + NADH + H+
-
-
-
r
decyl aldehyde + NADH + H+
1-decanol + NAD+
21% activity compared to benzyl alcohol
-
-
?
diacetyl + 2 NADH + 2 H+
2,3-butandiol + 2 NAD+
-
-
-
-
r
diacetyl + NADH + H+
acetoin + NAD+
150% of the activity with acetoin
-
-
?
diethylketone + NADH + H+
3-pentanol + NAD+
-
-
-
-
r
digitose + NAD+
? + NADH
-
-
-
-
?
dihydro-4,4-dimethyl-2,3-furandione + NADH + H+
?
-
13.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
dihydroxyacetone + NADH + H+
glycerol + NAD+
36% of the activity with acetoin
-
-
?
dihydroxyacetone phosphate + NADH + H+
glycerol phosphate + NAD+
82% of the activity with acetoin
-
-
?
DL-glyceraldehyde + H+
glycerol + NAD+
31% of the activity with acetoin
-
-
?
docosanol + NAD+
? + NADH + H+
-
immobilized HLAD shows about 60% reaction rate and free HLAD shows about 20% reaction rate with docosanol compared to ethanol at pH 8.8 and 30°C
-
-
?
dodecan-1-ol + NAD+
dodecanal + NADH + H+
-
-
-
r
dodecanol + NAD+
dodecanal + NADH + H+
-
immobilized HLAD shows about 60% reaction rate and free HLAD shows about 35% reaction rate with dodecanol compared to ethanol at pH 8.8 and 30°C
-
-
?
eicosan-1-ol + NAD+
n-eicosanal + NADH + H+
-
-
-
r
ethanol + 3-benzoylpyridine-adenine dinucleotide
acetaldehyde + ?
-
rapid equilibrium bi bi mechanism
-
-
?
ethanol + acycloNAD+
acetaldehyde + acycloNADH + H+
-
acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moietyhas been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety
-
-
?
ethanol + beta-NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
ethanol + NAD+
acetaldehyde + NADH + H+
ethanol + NAD+
aldehyde + NADH + H+
-
immobilized HLAD shows 100% reaction rate and free HLAD shows about 55% reaction rate with ethanol at pH 8.8 and 30°C
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
ethyl (2,4-dichlorophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(2,4-dichlorophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (3,5-difluorophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(3,5-difluorophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (4-bromophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(4-bromophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (4-chlorophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(4-chlorophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (4-cyanophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(4-cyanophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (4-fluorophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(4-fluorophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (4-methylphenyl)(oxo)acetate + NADH + H+
ethyl (2R)-hydroxy(4-methylphenyl)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl 2-oxopropanoate + NADPH + H+
(R)-ethyl 2-hydroxypropanoate + NADP+
294& of the activity with acetoin
-
-
r
ethyl 3,3-dimethyl-2-oxobutanoate + NADH + H+
ethyl (2R)-2-hydroxy-3,3-dimethylbutanoate + NAD+
enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 71% enantiomeric excess of ethyl (2S)-2-hydroxy-3,3-dimethylbutanoate
-
-
?
ethyl 3,3-dimethyl-2-oxobutanoate + NADH + H+
ethyl (2S)-2-hydroxy-3,3-dimethylbutanoate + NAD+
enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 71% enantiomeric excess of ethyl (2S)-2-hydroxy-3,3-dimethylbutanoate
-
-
?
ethyl 3-methyl-2-oxobutanoate + NADH + H+
ethyl (2R)-2-hydroxy-3-methylbutanoate + NAD+
enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 44% enantiomeric excess of ethyl (2S)-2-hydroxy-3-methylbutanoate
-
-
?
ethyl 3-methyl-2-oxobutanoate + NADH + H+
ethyl (2S)-2-hydroxy-3-methylbutanoate + NAD+
enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 44% enantiomeric excess of ethyl (2S)-2-hydroxy-3-methylbutanoate
-
-
?
ethyl 3-methyl-2-oxobutyrate + NADH + H+
?
1-phenyl-1,2-propanedione and ethyl 3-methyl-2-oxobutyrate are the best substrate in the reduction reaction
-
-
?
ethyl 3-methyl-2-oxobutyrate + NADH + H+
ethyl-2-hydroxy-3-methylbutyrate + NAD+
-
33% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl 3-oxo-3-phenylpropanoate + NADH + H+
ethyl (3S)-3-hydroxy-3-phenylpropanoate + NAD+
ethyl (3S)-3-hydroxy-3-phenylpropanoate is produced with 99% enantiomeric excess
-
-
?
ethyl 3-oxobutanoate + NADH
ethyl 3-hydroxybutanoate + NAD+
-
-
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl (3S)-3-hydroxybutanoate + NAD+
ethyl (3S)-3-hydroxybutanoate is produced with 95% enantiomeric excess
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
ethyl 3-oxobutyrate + NADH + H+
ethyl (S)-3-hydroxybutyrate + NAD+
-
-
-
-
?
ethyl 3-oxohexanoate + NADH
ethyl 3-hydroxyhexanoate + NAD+
-
-
-
-
?
ethyl 3-oxohexanoate + NADH + H+
ethyl (3R)-3-hydroxyhexanoate + NAD+
ethyl (3R)-3-hydroxyhexanoate is produced with 24% enantiomeric excess
-
-
?
ethyl 3-oxopentanoate + NADH
ethyl 3-hydroxypentanoate + NAD+
-
-
-
-
?
ethyl 3-oxopentanoate + NADH + H+
ethyl (3S)-3-hydroxypentanoate + NAD+
ethyl (3S)-3-hydroxypentanoate + NAD+is produced with 60% enantiomeric excess
-
-
?
ethyl 3-phenyl-3-oxopropanoate + NADH
ethyl 3-phenyl-3-hydroxypropanoate + NAD+
-
-
-
-
?
ethyl 4-bromo-3-oxobutanoate + NADH + H+
ethyl 4-bromo-3-hydroxybutanoate
-
511% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADH + H+
(S)-ethyl-4-chloro-3-hydroxybutanoate + NAD+
ethyl 4-chloro-3-oxobutanoate + NADH + H+
ethyl (3R)-4-chloro-3-hydroxybutanoate + NAD+
ethyl (3R)-4-chloro-3-hydroxybutanoate is produced with 4% enantiomeric excess
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADH + H+
ethyl 4-chloro-3-hydroxybutanoate + NAD+
-
809% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl 4-chloroacetoacetate + NADH + H+
?
-
6.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
ethyl 4-chloroacetoacetate + NADH + H+
ethyl 4-chloro-3-hydroxybutanoate + NAD+
-
-
-
-
r
ethyl 4-chloroacetoacetate + NADPH
?
activity is 1.48fold higher than with 2-hydroxyacetophenone and NADPH
-
-
?
ethyl 4-methyl-3-oxopentanoate + NADH + H+
ethyl (3S)-3-hydroxy-4-methylpentanoate + NAD+
ethyl (3S)-3-hydroxy-4-methylpentanoate is produced with 16% enantiomeric excess
-
-
?
ethyl acetoacetate + NADH + H+
?
-
42.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
ethyl acetoacetate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
-
-
-
r
ethyl benzoylformate + NADH
?
about 30% of the activity compared to isatin
-
-
?
ethyl benzoylformate + NADH
ethyl (R)-mandelate + NAD+
-
enantiomeric excess of 99.9%
-
-
?
ethyl benzoylformate + NADH + H+
?
23% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
ethyl benzoylformate + NADH + H+
ethyl (R)-(-)-mandelate + NAD+
-
95% enantiomeric excess
-
-
?
ethyl benzoylformate + NADH + H+
ethyl (R)-mandelate + NAD+
ethyl oxo(phenyl)acetate + NADH + H+
ethyl (2R)-hydroxy(phenyl)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl oxo(phenyl)acetate + NADH + H+
ethyl hydroxy(phenyl)acetate + NAD+
-
100% of the activity with 2,2,2-trifluoroacetophenone
-
-
r
ethyl pyruvate + NADH
ethyl 2-hydroxypropanoate + NAD+
-
-
-
-
?
ethyl pyruvate + NADH + H+
ethyl 2-hydroxypropanoate + NAD+
-
488% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl pyruvate + NADH + H+
ethyl 2-hydroxypropionate + NAD+
16% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
ethyl pyruvate + NADH + H+
ethyl lactate + NAD+
-
-
-
-
r
ethyl trifluoroacetoacetate + NADPH + H+
1-ethoxy-2,2,2-trifluoroethanol + NADP+
4.3% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
ethylene glycol + NAD+
? + NADH
ethylenglycol + NAD+
? + NADH
-
-
-
-
r
formaldehyde + NADH + H+
methanol + NAD+
furfural + NADH + H+
furfuryl alcohol + NAD+
furfuryl alcohol + NAD+
furfural + NADH
glutaraldehyde + NADH + H+
?
-
70.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
glutaraldehyde + NADH + H+
? + NAD+
best substrate in reduction reaction
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
glycolaldehyde + NADH + H+
glycol + NAD+
heptaldehyde + NADH + H+
? + NAD+
33% activity compared to benzyl alcohol
-
-
?
heptan-2-ol + NAD+
heptan-2-one + NADH
-
-
-
-
?
heptan-4-ol + NAD+
heptan-4-one + NADH
-
-
-
-
?
hexadecan-1-ol + NAD+
hexadecanal + NADH + H+
-
-
-
r
hexadecanol + NAD+
hexadecanal + NADH
-
very low activity
-
-
?
hexadecanol + NAD+
hexadecanal + NADH + H+
-
immobilized HLAD shows about 60% reaction rate and free HLAD shows about 15% reaction rate with hexadecanol compared to ethanol at pH 8.8 and 30°C
-
-
?
hexaldehyde + NADH + H+
1-hexanol + NAD+
7% activity compared to benzyl alcohol
-
-
?
hexan-1-ol + acycloNAD+
hexanal + acycloNADH + H+
-
acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety
-
-
?
hexan-1-ol + NAD+
hexanal + NADH + H+
hexan-1-ol + NAD+
n-hexanal + NADH
hexan-2-ol + NAD+
2-hexanone + NADH
-
-
-
-
?
hexanal + NADH + H+
1-hexanol + NAD+
69.6% of the activity with glutaraldehyde
-
-
?
hexanal + NADH + H+
hexan-1-ol + NAD+
16% of the activity with acetoin
-
-
?
hexanal + NADH + H+
n-hexanol + NAD+
hexanal + NADPH + H+
1-hexanone + NADP+
-
24% of the activity with NADH
-
-
?
hexanol + NAD+
hexaldehyde + NADH
-
-
-
-
?
hexanol + NAD+
hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
hydrocinnamaldehyde + NADH + H+
hydrocinnamyl alcohol + NAD+
12% activity compared to benzyl alcohol
-
-
r
hydrocinnamyl alcohol + NAD+
hydrocinnamaldehyde + NADH + H+
31% activity compared to benzyl alcohol
-
-
r
hydroxyacetone + NADH + H+
propane-1,2-diol + NAD+
-
27% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
isatin + NADH + H+
? + NAD+
iso-propanol + NAD+
? + NADH + H+
-
-
-
?
iso-propanol + NAD+
isopropanal + NADH + H+
isoamylalcohol + NAD+
3-methylbutanal + NADH + H+
-
-
-
r
isoborneol + NAD+
?
isoborneol is the best substrate in the oxidation reaction
-
-
?
isobutanal + NADH + H+
isobutanol + NAD+
20% of the activity with glutaraldehyde
-
-
?
isobutyl alcohol + NAD+
? + NADH
-
-
-
-
?
isobutyraldehyde + NADH + H+
isobutanol + NAD+
isobutyramide + NAD+
?
-
-
-
-
r
isopentan-1-ol + NAD+
isopentanal + NADH + H+
-
34% of the activity with ethanol
-
-
?
isopentanol + NAD+
? + NADH + H+
isopentenyl alcohol + NAD+
isopentanone + NADH
-
-
-
-
?
isopropanol + NAD+
2-propanone + NADH + H+
-
-
-
r
isopropanol + NAD+
acetone + NADH + H+
isopropanol + NAD+
propan-2-one + NADH + H+
-
-
-
r
L-glyceraldehyde + NAD+
?
-
-
-
?
levulinic acid + NADH + H+
?
-
11% of activity with N-benzyl-3-pyrrolidinone
-
-
?
m-chlorophenacyl chloride + NADH + H+
2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
4.6fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
m-nitrobenzaldehyde + NAD+
m-nitrobenzyl alcohol + NADH + H+
-
substrate of class IV ADH
-
-
?
m-nitrobenzaldehyde + NADH + H+
m-nitrobenzyl alcohol + NAD+
mannitol + NAD+
? + NADH + H+
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
methyl 3-oxobutanoate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
methyl 4-bromo-3-oxobutanoate + NADH + H+
methyl 4-bromo-3-hydroxybutanoate + NAD+
-
164% of the activity with phenyl trifluoromethyl ketone
-
-
?
methyl 4-chloro-3-oxobutanoate + NADH + H+
methyl 4-chloro-3-hydroxybutanoate + NAD+
methyl acetoacetate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
-
-
-
r
methyl benzoylformate + NADH
?
methyl benzoylformate + NADH + H+
methyl (R)-(-)-mandelate + NAD+
-
92% enantiomeric excess
-
-
?
methyl benzoylformate + NADH + H+
methyl (S)-mandelate + NAD+
-
6 h, 100% conversion, 17% enantiomeric excess
-
r
methyl glyoxal + NADH + H+
?
-
3.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
methyl o-chlorobenzoylformate + NADH
?
about 55% of the activity compared to isatin
-
-
?
methyl o-chlorobenzoylformate + NADH + H+
methyl (R)-o-chloromandelate + NAD+
-
6 h, 99% conversion, 72% enantiomeric excess
-
r
methyl oxo(phenyl)acetate + NADH + H+
methyl hydroxy(phenyl)acetate + NAD+
-
57% of the activity with 2,2,2-trifluoroacetophenone
-
-
r
methyl pyruvate + NADH + H+
methyl 2-hydroxypropanoate + NAD+
-
18.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
methyl pyruvate + NADH + H+
methyl lactate + NAD+
-
-
-
-
r
methylbenzoylformate + NADH + H+
?
-
13% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
methylcrotonyl alcohol + NAD+
methylcrotonaldehyde + NADH
-
-
-
-
?
methylglyoxal + NADH + H+
? + NAD+
-
11.9% of the activity with acetaldehyde
-
-
?
methylglyoxal + NADH + H+
acetol + NAD+
-
-
-
r
N-benzyl-3-piperidone + NADH + H+
N-benzylpiperidin-3-ol + NAD+
-
2.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
N-benzyl-3-pyrrolidinol + NAD+
1-benzylpyrrolidin-3-one + NADH + H+
-
33% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
N-benzyl-3-pyrrolidinone + NADH + H+
(R)-N-benzyl-3-pyrrolidinol + NAD+
-
(R)-stereoselectivity of the reduction carried out with the heat-treated cells
-
-
r
N-benzyl-3-pyrrolidinone + NADH + H+
(S)-N-benzyl-3-pyrrolidinol + NAD+
N-benzyl-4-piperidone + NADH + H+
N-benzylpiperidin-4-ol + NAD+
-
10.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
n-butanal + NADH + H+
n-butanol + NAD+
-
-
-
-
?
n-butanol + NAD+
butanal + NADH
-
low activity
-
-
?
n-butanol + NAD+
butylaldehyde + NADH + H+
n-butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
n-butanol + NAD+
butyraldehyde + NADH + H+
-
-
-
r
n-butanol + NAD+
n-butanal + NADH
n-butanol + NAD+
n-butanal + NADH + H+
-
-
-
-
?
n-butanol + NADH + H+
butyraldehyde + NAD+
-
-
-
r
n-butylaldehyde + NADH + H+
butanal + NAD+
-
activity is 1.8fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
n-butyraldehyde + NADH + H+
n-butanol + NAD+
-
7.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
n-decanol + NAD+
n-decanal + NADH
-
-
-
-
r
n-hexanol + NAD+
n-hexanal + NADH
-
-
-
-
r
n-hexylaldehyde + NADH + H+
hexanal + NAD+
-
activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
n-octylaldehyde + NADH + H+
n-octanol + NAD+
-
activity is 1.7fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
n-pentanol + NAD+
pentanal + NADH + H+
-
-
-
-
?
n-pentylaldehyde + NADH + H+
n-pentanol + NAD+
-
-
-
-
r
n-propanol + NAD+
n-propanal + NADH
-
-
-
-
r
n-propanol + NAD+
propanal + NADH
n-propanol + NAD+
propanal + NADH + H+
n-propanol + NAD+
propionaldehyde + NADH
-
-
-
-
?
N-tert-butoxycarbonyl-3-pyrrolidinone + NADH + H+
(S)-N-tert-butoxycarbonyl-3-pyrrolidinol + NAD+
-
production of (S)-N-tert-butoxycarbonyl-3-pyrrolidinol with 99.6% enantiomeric excess
-
-
?
n-valeraldehyde + NADH + H+
n-pentanol + NAD+
-
activity is 2.8fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
N-[4-(chloroacetyl)-2-nitrophenyl]acetamide + NADH + H+
N-[4-[(1R)-2-chloro-1-hydroxyethyl]-2-nitrophenyl]acetamide + NAD+
AE010289.1
production of N-[4-[(1R)-2-chloro-1-hydroxyethyl]-2-nitrophenyl]acetamide with 98% enantiomeric excess
-
-
?
nonyl aldehyde + NADH + H+
1-nonanol + NAD+
25% activity compared to benzyl alcohol
-
-
?
nonylphenol polyethoxylate 9 + NAD+
? + NADH
7% activity compared to benzyl alcohol
-
-
?
octacosan-1-ol + NAD+
octacosanal + NADH + H+
-
-
-
r
octadecan-1-ol + NAD+
octadecanal + NADH + H+
-
-
-
r
octan-1-ol + NAD+
n-octanal + NADH
octan-1-ol + NAD+
octanal + NADH + H+
-
-
-
r
octan-2-ol + NAD+
octan-2-one + NADH
octanal + NADH + H+
octanol + NAD+
octanol + NAD+
octanal + NADH
octanol + NAD+
octanal + NADH + H+
octyl aldehyde + NADH + H+
1-octanol + NAD+
29% activity compared to benzyl alcohol
-
-
?
octylphenol polyethoxylate 2 + NAD+
? + NADH
59% activity compared to benzyl alcohol
-
-
?
octylphenol polyethoxylate 8 + NAD+
? + NADH
7% activity compared to benzyl alcohol
-
-
?
oxalacetic acid + NADH + H+
?
-
14% of activity with N-benzyl-3-pyrrolidinone
-
-
?
oxaloacetate + NADH + H+
? + NAD+
-
-
-
-
r
p-chlorobenzaldehyde + NADH + H+
p-chlorobenzyl alcohol + NAD+
-
1.2fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
p-methoxybenzyl alcohol + NAD+
p-methoxybenzaldehyde + NADH + H+
p-nitrobenzaldehyde + NADH
p-nitrobenzyl alcohol + NAD+
-
-
-
-
?
p-nitrobenzaldehyde + NADH + H+
p-nitrobenzyl alcohol + NAD+
-
substrate of isozyme ADH4
-
-
r
p-nitrobenzyloxycarbonyl-3-pyrrolidinone + NADH + H+
(S)-N-p-nitrobenzyloxycarbonyl-3-pyrrolidinol + NAD+
-
production with 90.7% enantiomeric excess
-
-
?
p-nitrophenyl octanoate + H2O
p-nitrophenol + octanoate
-
acid-assisted nucleophilic catalysis involving the ammonium ion of Lys and the thiolate of Cys in the acyl-oxygen cleavage
-
?
pentan-1-ol + NAD+
pentanal + NADH + H+
pentan-2-ol + NAD+
2-pentanone + NADH
pentan-2-ol + NAD+
pentan-2-one + NADH + H+
pentan-3-ol + NAD+
3-pentanone + NADH
pentan-3-ol + NAD+
3-pentanone + NADH + H+
-
-
-
r
pentanal + NAD+
pentanone + NADH
pentanal + NADH
1-pentanol + NAD+ + H+
-
132% of the activity with phenyl trifluoromethyl ketone, in the reverse reaction 5% of the activity with 2-propanol
-
-
?
pentanal + NADH + H+
1-pentanol + NAD+
38% of the activity with glutaraldehyde
-
-
?
pentanol + NAD+
n-pentanal + NADH
pentanol + NAD+
pentanal + NADH + H+
pentanol + NAD+
valeraldehyde + NADH + H+
-
240% activity compared to cyclohexanone
-
r
phenyl trifluoromethyl ketone + NADH + H+
(S)-1-phenyltrifluoroethanol + NAD+
-
-
more than 99% enantiomeric excess
-
?
phenylacetaldehyde + NADH
phenylethanol + NAD+
phenylalaninol + NAD+
? + NADH
-
-
-
-
?
phenylethanol + NAD+
phenylacetaldehyde + NADH
-
R-(+)-phenylethanol and S-(-)-phenylethanol
-
-
?
phenylethanol + NAD+
phenylacetaldehyde + NADH + H+
phenylglyoxylic acid + NADH + H+
hydroxy(phenyl)acetic acid + NAD+
-
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde retains 48.77% activity of its original activity
-
-
?
phytol + NAD+
phytenal + NADH + H+
-
-
-
-
?
polyoxyethylene 8 decyl ether + NAD+
? + NADH
12% activity compared to benzyl alcohol
-
-
?
primary or secondary alcohol + NAD+
aldehyde or ketone + NADH
prop-2-en-1-ol + NAD+
? + NADH
-
140.6% of the activity with ethanol
-
-
?
prop-2-en-1-ol + NAD+
prop-2-enal + NADH + H+
-
85% of the activity with butan-2-ol
-
?
propan-1,2-diol + NAD+
? + NADH
propan-1-ol + acycloNAD+
propanal + acycloNADH + H+
-
acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety
-
-
?
propan-1-ol + NAD+
propanal + NADH + H+
propan-2-ol + NAD+
acetone + NADH
propan-2-ol + NAD+
acetone + NADH + H+
propanal + NADH + H+
propan-1-ol + NAD+
-
38% of the activity with butan-2-ol
-
r
propanal + NADH + H+
propanol + NAD+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
propionaldehyde + NADH
propanol + NAD+
propionaldehyde + NADH + H+
1-propanol + NAD+
-
-
-
-
r
propionaldehyde + NADH + H+
?
-
activity is 1.1fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
propiophenone + NADH + H+
1-phenylpropan-1-ol + NAD+
-
27% of activity with N-benzyl-3-pyrrolidinone
-
-
?
pyridine 2-aldehyde + NADH + H+
? + NAD+
-
-
-
-
r
pyridine 3-aldehyde + NADH + H+
? + NAD+
-
-
-
-
r
pyridine 4-aldehyde + NADH + H+
? + NAD+
-
-
-
-
r
pyridine-3-aldehyde + NADH + H+
pyridin-3-yl ethanol + NAD+
-
82% of activity with N-benzyl-3-pyrrolidinone
-
-
?
pyridine-4-aldehyde + NADH + H+
pyridin-4-yl methanol + NAD+
-
4.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
pyruvaldehyde + NADH + H+
lactaldehyde + NAD+
81% of the activity with acetoin
-
-
?
R-(+)-1-phenylethanol + NAD+
1-phenylethanone + NADH
-
-
-
-
?
R-(+)-cis-verbenol + NAD+
? + NADH
-
-
-
-
?
R-(+)-trans-bicyclo(2.2.1)-heptanol + NAD+
R-(+)-trans-bicyclo(2.2.1)-heptanal + NADH
-
R-(+)-trans-bicyclo(2.2.1)-heptanol and S-(-)-trans-bicyclo(2.2.1)-heptanol
-
-
?
rac-3-methylcyclohexanone + NADH + H+
(1S,3S)-3-methylcyclohexanol + NAD+
-
-
-
-
?
retinal + NADH + H+
retinol + NAD+
-
substrate of isozyme ADH4
-
-
r
retinol + NAD+
retinal + NADH
-
-
-
-
?
S-(-)-1-phenylethanol + NAD+
1-phenylethanone + NADH
-
-
-
-
?
S-(-)-cis-bicyclo(2.2.1)-heptanol + NAD+
S-(-)-cis-bicyclo(2.2.1)-heptanal + NADH
-
-
-
-
?
S-(-)-trans-bicyclo(2.2.1)-heptanol + NAD+
S-(-)-cis-bicyclo(2.2.1)-heptanal + NADH + H+
-
1054% activity compared to ethanol
-
-
r
secondary alcohol + NAD+
aldehyde + NADH
-
-
-
-
?
sinapaldehyde + NADH
sinapyl alcohol + NAD+
preferred substrates
-
-
r
sinapaldehyde + NADH + H+
sinalcohol + NAD+
sinapaldehyde + NADH + H+
sinapyl alcohol + NAD+
preferred substrates
-
-
r
tert-butanol + NAD+
butyraldehyde + NADH + H+
-
-
-
?
tert-butyl 3-oxobutanoate + NADH + H+
tert butyl 3-hydroxybutanoate + NAD+
-
568% of the activity with phenyl trifluoromethyl ketone
-
-
?
tert-butyl acetoacetate + NADH + H+
?
-
-
-
?
tetracosanol + NAD+
? + NADH + H+
-
immobilized HLAD shows about 55% reaction rate and free HLAD shows about 15% reaction rate with tetracosanol compared to ethanol at pH 8.8 and 30°C
-
-
?
tetracosanol-1-ol + NAD+
tetracosanal + NADH + H+
-
-
-
r
tetradecan-1-ol + NAD+
tetradecanal + NADH + H+
-
-
-
r
tetrahydro-4H-pyran-4-one + NADH + H+
? + NAD+
10% activity compared to cyclohexanone
-
-
?
tetralin-1-ol + NAD+
3,4-dihydronaphthalen-1(2H)-one + NADH + H+
51% of the activity compared to isoborneol
-
-
?
trans-4-(N,N-dimethylamino)-cinnamaldehyde + NADH
trans-4-(N,N-dimethylamino)-cinnamyl alcohol + NAD+
-
-
-
-
?
trans-4-methylcyclohexanol + NAD+
4-methylcyclohexanone + NADH
-
-
-
-
?
trans-cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
trans-cinnamyl alcohol + NAD+
(2E)-3-phenylprop-2-enal + NADH + H+
-
activity is 3.9fold higher than with (S)-(-)-1-phenylethanol
-
-
?
triacontan-1-ol + NAD+
triacontanal + NADH + H+
-
-
-
r
Tris + NAD+
? + NADH
-
-
-
-
r
Triton X-100 + NAD+
? + NADH
7% activity compared to benzyl alcohol
-
-
?
Triton X-114 + NAD+
? + NADH
3% activity compared to benzyl alcohol
-
-
?
Triton X-165 + NAD+
? + NADH
24% activity compared to benzyl alcohol
-
-
?
Triton X-35 + NAD+
? + NADH
43% activity compared to benzyl alcohol
-
-
?
Triton X-405 + NAD+
? + NADH
19% activity compared to benzyl alcohol
-
-
?
Triton X-45 + NAD+
? + NADH
7% activity compared to benzyl alcohol
-
-
?
tryptophol + NAD+
? + NADH
-
-
-
-
?
ubiquinone + NADH + H+
ubiquinol + NAD+
valeraldehyde + NADH
1-pentanol + NAD+
vanillyl alcohol + NAD+
vanillin + NADH
-
-
-
-
?
additional information
?
-
(E)-hex-2-en-1-ol + NAD+
(E)-hex-2-en-1-one + NADH
-
43.2% of the activity with ethanol
-
-
?
(E)-hex-2-en-1-ol + NAD+
(E)-hex-2-en-1-one + NADH
-
-
-
-
?
(R)-1-phenyl-1-butanol + NAD+
1-phenylbutan-1-one + NADH + H+
-
no substrate of mutant B1
-
-
r
(R)-1-phenyl-1-butanol + NAD+
1-phenylbutan-1-one + NADH + H+
-
no substrate of mutant B1
-
-
r
(R)-1-phenylethanol + NAD+
acetophenone + NADH + H+
-
-
-
-
r
(R)-1-phenylethanol + NAD+
acetophenone + NADH + H+
-
-
-
-
r
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
55% of activity with N-benzyl-3-pyrrolidinol
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
55% of activity with N-benzyl-3-pyrrolidinol
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
50% of the activity with 2-propanol
-
-
ir
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
50% of the activity with 2-propanol
-
-
ir
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
r
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
(R)-2-heptanol + NAD+
2-heptanone + NADH + H+
-
2469% of the activity with 2-propanol
-
-
r
(R)-2-heptanol + NAD+
2-heptanone + NADH + H+
-
2469% of the activity with 2-propanol
-
-
r
(R)-2-hexanol + NAD+
2-hexanone + NADH + H+
-
1906% of the activity with 2-propanol
-
-
r
(R)-2-hexanol + NAD+
2-hexanone + NADH + H+
-
1906% of the activity with 2-propanol
-
-
r
(R)-2-octanol + NAD+
2-octanone + NADH + H+
-
-
-
-
?
(R)-2-octanol + NAD+
2-octanone + NADH + H+
-
2406% of the activity with 2-propanol
-
-
r
(R)-2-octanol + NAD+
2-octanone + NADH + H+
-
2406% of the activity with 2-propanol
-
-
r
(R)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
-
-
-
?
(R)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
75% of the activity with 2-propanol
-
-
r
(R)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
-
-
r
(R)-2-phenylpropanol + NAD+
(R)-2-phenylpropanal + NADH + H+
-
63% of the activity with 2-phenylethanol
-
-
?
(R)-2-phenylpropanol + NAD+
(R)-2-phenylpropanal + NADH + H+
-
63% of the activity with 2-phenylethanol
-
-
?
(R,S)-2-methylbutan-1-ol + NAD+
(R,S)-2-methyl-butan-1-one + NADH + H+
-
-
-
-
?
(R,S)-2-methylbutan-1-ol + NAD+
(R,S)-2-methyl-butan-1-one + NADH + H+
-
-
-
?
(R,S)-2-methylbutan-1-ol + NAD+
(R,S)-2-methyl-butan-1-one + NADH + H+
-
-
-
?
(S)-1-indanol + NAD+
1-indanone + NADH + H+
-
-
-
r
(S)-1-indanol + NAD+
1-indanone + NADH + H+
-
3900% of the activity with (S)-1-phenylethanol
-
-
r
(S)-1-phenylethanol + NAD+
acetophenone + NADH + H+
-
-
-
-
r
(S)-1-phenylethanol + NAD+
acetophenone + NADH + H+
-
-
-
-
r
(S)-2-butanol + NAD+
2-butanone + NADH + H+
-
275% of the activity with 2-propanol
-
-
ir
(S)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
r
(S)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
?
(S)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
(S)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
(S)-2-butanol + NAD+
butanone + NADH + H+
-
-
-
-
r
(S)-2-butanol + NAD+
butanone + NADH + H+
-
-
-
-
r
(S)-2-methylbutan-1-ol + NAD+
(S)-2-methyl-butanal + NADH + H+
-
-
-
-
?
(S)-2-methylbutan-1-ol + NAD+
(S)-2-methyl-butanal + NADH + H+
-
-
-
?
(S)-2-methylbutan-1-ol + NAD+
(S)-2-methyl-butanal + NADH + H+
-
-
-
-
?
(S)-2-octanol + NAD+
2-octanone + NADH + H+
-
-
-
-
?
(S)-2-octanol + NAD+
2-octanone + NADH + H+
-
81% of the activity with 2-propanol
-
-
r
(S)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
-
-
-
?
(S)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
25% of the activity with 2-propanol
-
-
r
(S)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
-
-
r
(S)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
-
-
-
r
(S)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
-
-
-
r
(S)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
-
2700% of the activity with (S)-1-phenylethanol
-
-
r
1,2-butanediol + NAD+
?
-
35% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1,2-butanediol + NAD+
?
low activity
-
-
?
1,2-butanediol + NAD+
?
low activity
-
-
?
1,2-butanediol + NAD+
? + NADH + H+
-
5% activity compared to 1-propanol
-
-
?
1,2-butanediol + NAD+
? + NADH + H+
-
-
-
?
1,2-propanediol + NAD+
?
-
-
-
-
?
1,2-propanediol + NAD+
?
-
-
-
-
?
1,3-propanediol + NAD+
?
-
-
-
-
?
1,3-propanediol + NAD+
?
-
-
-
-
?
1,3-propanediol + NAD+
? + NADH + H+
-
-
-
?
1,3-propanediol + NAD+
? + NADH + H+
-
-
-
?
1-(4-fluorophenyl)ethanol + NAD+
1-(4-fluorophenyl)ethanone + NADH + H+
119% of the activity with 1-phenylethanol
-
-
?
1-(4-fluorophenyl)ethanol + NAD+
1-(4-fluorophenyl)ethanone + NADH + H+
-
45% of the activity with (S)-(-)-1-phenylethanol
-
-
?
1-butanol + NAD+
butanal + NADH
-
111% of the activity with 2-phenylethanol
-
-
?
1-butanol + NAD+
butanal + NADH
-
111% of the activity with 2-phenylethanol
-
-
?
1-butanol + NAD+
butanal + NADH
-
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
34% activity compared to 1-propanol
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
34% activity compared to 1-propanol
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
15.7% of the activity with ethanol
-
-
?
1-butanol + NAD+
butanal + NADH + H+
about 80% of activity with ethanol, ADH1
-
-
?
1-butanol + NAD+
butanal + NADH + H+
about 80% of activity with ethanol, ADH1
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
30% activity compared to benzyl alcohol
-
-
?
1-butanol + NAD+
butanal + NADH + H+
30% activity compared to benzyl alcohol
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
67.7% activity compared to ethanol
-
-
?
1-butanol + NAD+
butanal + NADH + H+
142% activity compared to cyclohexanol
-
-
r
1-butanol + NAD+
butanal + NADH + H+
142% activity compared to cyclohexanol
-
-
r
1-butanol + NAD+
butyraldehyde + NADH + H+
-
-
-
?
1-butanol + NAD+
butyraldehyde + NADH + H+
-
-
-
?
1-decanol + NAD+
decanal + NADH + H+
-
-
-
r
1-decanol + NAD+
decanal + NADH + H+
-
-
-
r
1-decanol + NAD+
decanal + NADH + H+
13% activity compared to benzyl alcohol
-
-
?
1-decanol + NAD+
decanal + NADH + H+
13% activity compared to benzyl alcohol
-
-
?
1-dodecanol + NAD+
dodecanal + NADH
-
68% of the activity with 2-phenylethanol
-
-
?
1-dodecanol + NAD+
dodecanal + NADH
-
68% of the activity with 2-phenylethanol
-
-
?
1-heptanol + NAD+
heptanal + NADH + H+
-
-
-
r
1-heptanol + NAD+
heptanal + NADH + H+
-
-
-
r
1-heptanol + NAD+
heptanal + NADH + H+
-
56% of the activity with 2-propanol, in the reverse reaction 755% of the activity with phenyl trifluoromethyl ketone
-
-
r
1-heptanol + NAD+
heptanal + NADH + H+
-
-
-
?
1-heptanol + NAD+
heptanal + NADH + H+
-
-
-
?
1-heptanol + NAD+
heptanal + NADH + H+
25% activity compared to benzyl alcohol
-
-
?
1-heptanol + NAD+
heptanal + NADH + H+
25% activity compared to benzyl alcohol
-
-
?
1-heptanol + NAD+
heptanal + NADH + H+
80% activity compared to cyclohexanol
-
-
r
1-heptanol + NAD+
heptanal + NADH + H+
80% activity compared to cyclohexanol
-
-
r
1-hexanol + NAD+
1-hexanal + NADH + H+
-
-
-
?
1-hexanol + NAD+
1-hexanal + NADH + H+
-
-
-
r
1-hexanol + NAD+
hexanal + NADH + H+
-
7% activity compared to 1-propanol
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
-
7% activity compared to 1-propanol
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
-
-
-
r
1-hexanol + NAD+
hexanal + NADH + H+
-
44% of the activity with 2-propanol, in the reverse reaction 1029% of the activity with phenyl trifluoromethyl ketone
-
-
r
1-hexanol + NAD+
hexanal + NADH + H+
about 90% of activity with ethanol, ADH1
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
about 90% of activity with ethanol, ADH1
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
-
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
-
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
61% activity compared to benzyl alcohol
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
61% activity compared to benzyl alcohol
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
-
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
-
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
-
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
15.3% activity compared to ethanol
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
109% activity compared to cyclohexanol
-
-
r
1-indanol + NAD+
1-indanone + NADH + H+
26% of the activity compared to isoborneol
-
-
?
1-indanol + NAD+
1-indanone + NADH + H+
26% of the activity compared to isoborneol
-
-
?
1-indanol + NAD+
1-indanone + NADH + H+
-
1% activity compared to cyclohexanone
-
r
1-indanol + NAD+
1-indanone + NADH + H+
-
-
-
-
?
1-indanone + NADH + H+
(S)-1-indanol + NAD+
-
6 h, 50°C, 6% conversion, 25% enantiomeric excess
-
r
1-indanone + NADH + H+
(S)-1-indanol + NAD+
-
6 h, 50°C, 6% conversion, 25% enantiomeric excess
-
r
1-nonanol + NAD+
nonanal + NADH + H+
-
-
-
r
1-nonanol + NAD+
nonanal + NADH + H+
12% activity compared to benzyl alcohol
-
-
?
1-octanol + NAD+
octanal + NADH
-
101% of the activity with 2-phenylethanol
-
-
?
1-octanol + NAD+
octanal + NADH
-
101% of the activity with 2-phenylethanol
-
-
?
1-octanol + NAD+
octanal + NADH + H+
-
-
-
r
1-octanol + NAD+
octanal + NADH + H+
substrate for isozyme ADH3
-
-
r
1-octanol + NAD+
octanal + NADH + H+
-
33% of the activity with 2-propanol, in the reverse reaction 435% of the activity with phenyl trifluoromethyl ketone
-
-
r
1-octanol + NAD+
octanal + NADH + H+
about 85% of activity with ethanol, ADH1
-
-
?
1-octanol + NAD+
octanal + NADH + H+
-
-
-
r
1-octanol + NAD+
octanal + NADH + H+
-
-
-
?
1-octanol + NAD+
octanal + NADH + H+
-
-
-
?
1-octanol + NAD+
octanal + NADH + H+
11% activity compared to benzyl alcohol
-
-
?
1-octanol + NAD+
octanal + NADH + H+
-
-
-
?
1-octanol + NAD+
octanal + NADH + H+
-
-
-
?
1-octanol + NAD+
octanal + NADH + H+
57% activity compared to cyclohexanol
-
-
r
1-pentanol + NAD+
1-pentanal + NADH + H+
-
-
-
?
1-pentanol + NAD+
1-pentanal + NADH + H+
-
-
-
r
1-pentanol + NAD+
pentanal + NADH + H+
-
-
-
r
1-pentanol + NAD+
pentanal + NADH + H+
-
8% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1-pentanol + NAD+
pentanal + NADH + H+
about 85% of activity with ethanol, ADH1
-
-
?
1-pentanol + NAD+
pentanal + NADH + H+
-
-
-
r
1-pentanol + NAD+
pentanal + NADH + H+
-
-
-
?
1-pentanol + NAD+
pentanal + NADH + H+
152% activity compared to benzyl alcohol
-
-
?
1-pentanol + NAD+
pentanal + NADH + H+
-
-
-
?
1-pentanol + NAD+
pentanal + NADH + H+
19.1% activity compared to ethanol
-
-
?
1-pentanol + NAD+
pentanal + NADH + H+
121% activity compared to cyclohexanol
-
-
r
1-phenyl-1,2-propanedione + NADH + H+
?
1-phenyl-1,2-propanedione and ethyl 3-methyl-2-oxobutyrate are the best substrate in the reduction reaction
-
-
?
1-phenyl-1,2-propanedione + NADH + H+
?
1-phenyl-1,2-propanedione and ethyl 3-methyl-2-oxobutyrate are the best substrate in the reduction reaction
-
-
?
1-phenyl-1,2-propanedione + NADH + H+
?
-
activity is 1.46fold higher than with 2,2,2-trifluoroacetophenone
-
-
?
1-phenyl-2-butanone + NADH + H+
1-phenylbutan-2-ol + NAD+
-
1.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
1-phenyl-2-butanone + NADH + H+
1-phenylbutan-2-ol + NAD+
-
1.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
1-phenyl-2-butanone + NADH + H+
1-phenylbutan-2-ol + NAD+
-
12% of the activity with phenyl trifluoromethyl ketone
-
-
?
1-phenyl-2-propanol + NAD+
1-phenyl-2-propanone + NADH + H+
39% of the activity with 1-phenylethanol
-
-
?
1-phenyl-2-propanol + NAD+
1-phenyl-2-propanone + NADH + H+
3% activity compared to cyclohexanol
-
-
r
1-phenyl-2-propanol + NAD+
1-phenyl-2-propanone + NADH + H+
-
16% of the activity with (S)-(-)-1-phenylethanol
-
-
?
1-phenylethanol + NAD+
1-phenylethanone + NADH
-
46% of the activity with 2-phenylethanol
-
-
?
1-phenylethanol + NAD+
1-phenylethanone + NADH
-
-
-
?
1-phenylethanol + NAD+
1-phenylethanone + NADH + H+
-
-
-
?
1-phenylethanol + NAD+
1-phenylethanone + NADH + H+
3% activity compared to cyclohexanol
-
-
r
1-propanol + NAD+
propanal + NADH + H+
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
-
-
-
r
1-propanol + NAD+
propanal + NADH + H+
the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol
-
-
?
1-propanol + NAD+
propanal + NADH + H+
the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol
-
-
?
1-propanol + NAD+
propanal + NADH + H+
-
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
about 75% of activity with ethanol, ADH1
-
-
?
1-propanol + NAD+
propanal + NADH + H+
-
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
-
-
-
r
1-propanol + NAD+
propanal + NADH + H+
-
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
153% activity compared to cyclohexanol
-
-
r
1-propanol + NAD+
propanaldehyde + NADH + H+
-
-
-
-
r
1-propanol + NAD+
propanaldehyde + NADH + H+
-
-
-
r
11-cis-retinol + NAD+
11-cis-retinal + NADH
-
-
-
?
11-cis-retinol + NAD+
11-cis-retinal + NADH
-
-
-
-
?
11-cis-retinol + NAD+
11-cis-retinal + NADH
-
-
-
-
?
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
-
-
-
-
?
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
-
-
-
-
?
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
-
-
-
-
?
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
-
oxidized at pH 10, not oxidized at pH 7.5
-
-
?
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
-
-
-
-
?
13-cis-retinol + NAD+
13-cis-retinal + NADH
-
-
-
?
13-cis-retinol + NAD+
13-cis-retinal + NADH
-
no activity with isozyme ADH1
-
-
?
13-cis-retinol + NAD+
13-cis-retinal + NADH
-
no activity with isozyme ADH1
-
-
?
2,2,2-trifluoroacetophenone + NADH + H+
(R)-alpha-(trifluoromethyl)benzyl alcohol + NAD+
-
-
93% enantiomeric excess
-
r
2,2,2-trifluoroacetophenone + NADH + H+
(R)-alpha-(trifluoromethyl)benzyl alcohol + NAD+
-
93% enantiomeric excess
-
-
?
2,2,2-trifluoroacetophenone + NADH + H+
2,2,2-trifluoro-1-phenylethanol + NAD+
35% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
2,2,2-trifluoroacetophenone + NADH + H+
2,2,2-trifluoro-1-phenylethanol + NAD+
-
-
-
-
r
2,2-dichloroacetophenone + NADH + H+
2,2-dichloro-1-phenylethanol + NAD+
30% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
2,2-dichloroacetophenone + NADH + H+
2,2-dichloro-1-phenylethanol + NAD+
-
32% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
2,3'-dichloroacetophenone + NADH + H+
2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
67% of the activity with phenyl trifluoromethyl ketone
-
-
?
2,3'-dichloroacetophenone + NADH + H+
2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
-
-
-
r
2,3'-dichloroacetophenone + NADH + H+
2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
-
-
-
r
2,3-butanediol + NAD+
acetoin + NADH + H+
-
-
-
-
?
2,3-butanediol + NAD+
acetoin + NADH + H+
-
-
-
?
2,3-butanediol + NAD+
acetoin + NADH + H+
-
the enzyme is most active with 2,3-butanediol, preference for NADH in the reductive direction. Insertion of an RTX domain from the adenylate cyclase of Bordetella pertussis into a loop near the catalytic active site of the thermostable alcohol dehydrogenase D from Pyrococcus furiosus. The resultant chimera, beta-AdhD, gains the calcium-binding ability of the beta-roll, retains the thermostable activity of AdhD, and exhibits reduced overall alcohol dehydrogenase activity. The addition of calcium to beta-AdhD preferentially inhibits NAD+-dependent activity in comparison to NADP+-dependent activity. Calcium is a competitive inhibitor of AdhD, and the addition of the RTX domain introduces calcium-dependent noncompetitive inhibition to beta-AdhD affecting NAD+-dependent activity
-
-
?
2,3-butanediol + NAD+
acetoin + NADH + H+
272% of the activity with 1-phenylethanol
-
-
r
2,3-butanediol + NADP+
acetoin + NADPH + H+
-
-
-
-
?
2,3-butanediol + NADP+
acetoin + NADPH + H+
-
the enzyme is most active with 2,3-butanediol, preference for NADH in the reductive direction. Isertion of an RTX domain from the adenylate cyclase of Bordetella pertussis into a loop near the catalytic active site of the thermostable alcohol dehydrogenase D from Pyrococcus furiosus. The resultant chimera, beta-AdhD, gains the calcium-binding ability of the beta-roll, retains the thermostable activity of AdhD, and exhibits reduced overall alcohol dehydrogenase activity. The addition of calcium to beta-AdhD preferentially inhibits NAD+-dependent activity in comparison to NADP+-dependent activity. Calcium is a competitive inhibitor of AdhD, and the addition of the RTX domain introduces calcium-dependent noncompetitive inhibition to beta-AdhD affecting NAD+-dependent activity
-
-
?
2,4-pentanediol + NAD+
?
-
21% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
2,4-pentanediol + NAD+
?
4% activity compared to cyclohexanol
-
-
?
2-(3,5-dimethylphenyl)propanal + NADH + H+
(S)-2-(3,5-dimethylphenyl)propanol + NAD+
18 h, 99% yield, 90% enantiomeric excess
-
-
?
2-(3,5-dimethylphenyl)propanal + NADH + H+
(S)-2-(3,5-dimethylphenyl)propanol + NAD+
18 h, 99% yield, 90% enantiomeric excess
-
-
?
2-(3-benzoylphenyl)propanal + NADH + H+
(S)-2-(3-benzoylphenyl)propanol + NAD+
18 h, 85% yield, 95% enantiomeric excess
-
-
?
2-(3-benzoylphenyl)propanal + NADH + H+
(S)-2-(3-benzoylphenyl)propanol + NAD+
18 h, 85% yield, 95% enantiomeric excess
-
-
?
2-(3-fluorobiphenyl-4-yl)propanal + NADH + H+
(S)-2-(3-(fluoro)biphenyl-4-yl)propanol + NAD+
18 h, 77% yield, 97% enantiomeric excess
-
-
?
2-(3-fluorobiphenyl-4-yl)propanal + NADH + H+
(S)-2-(3-(fluoro)biphenyl-4-yl)propanol + NAD+
18 h, 77% yield, 97% enantiomeric excess
-
-
?
2-(3-phenoxyphenyl)propanal + NADH + H+
(S)-2-((3-phenoxy)phenyl)propanol + NAD+
18 h, 85% yield, 95% enantiomeric excess
-
-
?
2-(3-phenoxyphenyl)propanal + NADH + H+
(S)-2-((3-phenoxy)phenyl)propanol + NAD+
18 h, 85% yield, 95% enantiomeric excess
-
-
?
2-(4-isobutylphenyl)propanal + NADH + H+
(S)-2-(4-isobutylphenyl)propanol + NAD+
18 h, 92% yield, 99% enantiomeric excess
-
-
?
2-(4-isobutylphenyl)propanal + NADH + H+
(S)-2-(4-isobutylphenyl)propanol + NAD+
18 h, 92% yield, 99% enantiomeric excess
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
weak activity
-
-
r
2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
-
activity is 3.7fold higher than with (S)-N-benzyl-3-pyrrolidinol
-
-
r
2-butanol + NAD+
2-butanone + NADH + H+
about 25% of activity with ethanol, ADH1
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
r
2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
r
2-butanol + NAD+
2-butanone + NADH + H+
39% activity compared to cyclohexanol
4% activity compared to cyclohexanone
-
r
2-butanone + NADH + H+
2-butanol + NAD+
-
40% of the activity with N-benzyl-3-pyrrolidinone
-
-
r
2-butanone + NADH + H+
2-butanol + NAD+
-
-
-
-
r
2-butene-1-ol + NAD+
? + NADH
-
-
-
-
?
2-butene-1-ol + NAD+
? + NADH
-
-
-
-
?
2-heptanol + NAD+
2-heptanone + NADH + H+
weak activity
-
-
r
2-heptanol + NAD+
2-heptanone + NADH + H+
63% activity compared to cyclohexanol
5% activity compared to cyclohexanone
-
r
2-hexanol + NAD+
2-hexanone + NADH + H+
weak activity
-
-
r
2-hexanol + NAD+
2-hexanone + NADH + H+
-
activity is 11.2fold higher than with (S)-N-benzyl-3-pyrrolidinol
-
-
?
2-hexanol + NAD+
2-hexanone + NADH + H+
64% activity compared to cyclohexanol
4% activity compared to cyclohexanone
-
r
2-mercaptoethanol + NAD+
?
-
-
-
-
?
2-mercaptoethanol + NAD+
?
11% activity compared to cyclohexanol
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
-
-
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
Sporotrichum pulverulentum
-
-
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
-
-
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
-
-
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
-
-
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
-
weak activity
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH + H+
-
-
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH + H+
-
-
-
?
2-octanol + NAD+
2-octanone + NADH + H+
weak activity
-
-
r
2-octanol + NAD+
2-octanone + NADH + H+
low activity
-
-
r
2-octanol + NAD+
2-octanone + NADH + H+
43% activity compared to cyclohexanol
4% activity compared to cyclohexanone
-
r
2-oxo-3-methylpentane + NADH
3-methylpentan-2-ol + NAD+
-
-
-
-
?
2-oxo-3-methylpentane + NADH
3-methylpentan-2-ol + NAD+
-
-
-
-
?
2-oxo-3-phenylpropane + NADH
2-hydroxy-3-phenylpropane + NAD+
-
-
-
-
?
2-oxo-3-phenylpropane + NADH
2-hydroxy-3-phenylpropane + NAD+
-
-
-
-
?
2-pentanol + NAD+
2-pentanone + NADH + H+
-
-
-
?
2-pentanol + NAD+
2-pentanone + NADH + H+
-
45% of activity with N-benzyl-3-pyrrolidinol
-
-
?
2-pentanol + NAD+
2-pentanone + NADH + H+
-
45% of activity with N-benzyl-3-pyrrolidinol
-
-
?
2-pentanol + NAD+
2-pentanone + NADH + H+
weak activity
-
-
r
2-pentanol + NAD+
2-pentanone + NADH + H+
-
activity is 9fold higher than with (S)-N-benzyl-3-pyrrolidinol
-
-
?
2-pentanol + NAD+
2-pentanone + NADH + H+
low activity
-
-
r
2-pentanol + NAD+
2-pentanone + NADH + H+
5.7% activity compared to ethanol
-
-
?
2-pentanone + NADH + H+
2-pentanol + NAD+
-
-
-
-
?
2-pentanone + NADH + H+
2-pentanol + NAD+
-
-
-
?
2-pentanone + NADH + H+
2-pentanol + NAD+
-
-
-
-
?
2-pentanone + NADH + H+
2-pentanol + NAD+
-
-
-
?
2-pentanone + NADH + H+
2-pentanol + NAD+
-
-
-
?
2-pentanone + NADH + H+
2-pentanol + NAD+
-
-
-
?
2-pentanone + NADH + H+
2-pentanol + NAD+
6% activity compared to cyclohexanol
-
-
r
2-pentanone + NADH + H+
2-pentanol + NAD+
-
-
-
-
r
2-propanol + NAD(P)+
acetone + NAD(P)H
-
-
-
-
?
2-propanol + NAD(P)+
acetone + NAD(P)H
-
-
-
-
?
2-propanol + NAD+
2-propanone + NADH
-
54% of the activity with 2-phenylethanol
-
-
?
2-propanol + NAD+
2-propanone + NADH
-
-
-
-
?
2-propanol + NAD+
2-propanone + NADH
-
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
-
activity is 3.1fold higher than with (S)-N-benzyl-3-pyrrolidinol
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
-
-
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
-
-
-
r
2-propanol + NAD+
2-propanone + NADH + H+
-
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
-
-
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
-
-
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
73.5% activity compared to ethanol
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
6% activity compared to cyclohexanol
-
-
r
2-propanol + NAD+
acetone + NADH + H+
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
r
2-propanol + NAD+
acetone + NADH + H+
-
-
-
r
2-propanol + NAD+
acetone + NADH + H+
-
-
-
-
ir
2-propanol + NAD+
acetone + NADH + H+
about 50% of activity with ethanol, ADH1
-
-
?
2-propanol + NAD+
acetone + NADH + H+
about 50% of activity with ethanol, ADH1
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
-
?
3,4-dimethylbenzyl alcohol + NAD+
3,4-dimethylbenzaldehyde + NADH + H+
45% activity compared to cyclohexanol
-
-
r
3,4-dimethylbenzyl alcohol + NAD+
3,4-dimethylbenzaldehyde + NADH + H+
45% activity compared to cyclohexanol
-
-
r
3-methoxybenzyl alcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
-
-
-
-
?
3-methoxybenzyl alcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
-
13% of the activity with (S)-(-)-1-phenylethanol
-
-
?
3-methoxybenzyl alcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
-
13% of the activity with (S)-(-)-1-phenylethanol
-
-
?
3-methylbutan-1-ol + NAD+
3-methyl-butan-1-one + NADH + H+
-
-
-
-
?
3-methylbutan-1-ol + NAD+
3-methyl-butan-1-one + NADH + H+
-
-
-
?
3-methylbutanal + NAD+
? + NADH + H+
about 10% of the activity with ethanol or 1-propanol
-
-
?
3-methylbutanal + NAD+
? + NADH + H+
about 10% of the activity with ethanol or 1-propanol
-
-
?
3-methylbutanol + NAD+
? + NADH
-
-
-
-
?
3-methylbutanol + NAD+
? + NADH
-
-
-
-
?
3-methylcyclohexanone + NADH
3-methylcyclohexanol + NAD+
-
-
-
-
r
3-methylcyclohexanone + NADH
3-methylcyclohexanol + NAD+
about 10% of the activity compared to isatin
-
-
?
3-methylcyclohexanone + NADH
3-methylcyclohexanol + NAD+
about 10% of the activity compared to isatin
-
-
?
3-nitrobenzaldehyde + NADH + H+
3-nitrobenzyl alcohol + NAD+
-
class IV isozyme, reductive activity
-
-
?
3-nitrobenzaldehyde + NADH + H+
3-nitrobenzyl alcohol + NAD+
-
-
-
-
r
3-oxo-5beta-androstan-17beta-ol + NADH
3beta,17beta-dihydroxy-5beta-androstane + NAD+
-
-
-
-
?
3-oxo-5beta-androstan-17beta-ol + NADH
3beta,17beta-dihydroxy-5beta-androstane + NAD+
-
-
-
-
?
3-pentanol + NAD+
3-pentanone + NADH + H+
-
-
-
r
3-pentanol + NAD+
3-pentanone + NADH + H+
2% activity compared to cyclohexanol
2% activity compared to cyclohexanone
-
r
3beta-hydroxy-5beta-androstan-17-one + NAD+
5beta-androstan-3,17-dione + NADH
-
-
-
-
?
3beta-hydroxy-5beta-androstan-17-one + NAD+
5beta-androstan-3,17-dione + NADH
-
catalyzed by isoenzyme BB-ADH, no activity with isoenzyme AA-ADH and TT-ADH
-
-
?
3beta-hydroxy-5beta-androstan-17-one + NAD+
5beta-androstan-3,17-dione + NADH
-
-
-
-
?
4-bromoacetophenone + NADH + H+
1-(4-bromophenyl)ethanol + NAD+
-
-
-
-
?
4-bromoacetophenone + NADH + H+
1-(4-bromophenyl)ethanol + NAD+
-
-
-
?
4-bromoacetophenone + NADH + H+
1-(4-bromophenyl)ethanol + NAD+
-
-
-
-
?
4-bromoacetophenone + NADH + H+
1-(4-bromophenyl)ethanol + NAD+
-
-
-
?
4-bromoacetophenone + NADH + H+
1-(4-bromophenyl)ethanol + NAD+
-
-
-
?
4-chloroacetophenone + NADH
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
-
r
4-chloroacetophenone + NADH
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
-
r
4-chloroacetophenone + NADH
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
-
r
4-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
-
?
4-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
?
4-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
-
?
4-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
?
4-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
?
4-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
-
r
4-deoxy-L-erythro-5-hexoseulose + NAD+
2-dehydro-3-deoxy-D-gluconate + NADH + H+
100% activity
-
-
r
4-deoxy-L-erythro-5-hexoseulose + NAD+
2-dehydro-3-deoxy-D-gluconate + NADH + H+
preferred reaction
-
-
r
4-fluoroacetophenone + NADH + H+
1-(4-fluorophenyl)ethanol + NAD+
-
-
-
-
?
4-fluoroacetophenone + NADH + H+
1-(4-fluorophenyl)ethanol + NAD+
-
-
-
?
4-fluoroacetophenone + NADH + H+
1-(4-fluorophenyl)ethanol + NAD+
-
-
-
-
?
4-fluoroacetophenone + NADH + H+
1-(4-fluorophenyl)ethanol + NAD+
-
-
-
?
4-fluoroacetophenone + NADH + H+
1-(4-fluorophenyl)ethanol + NAD+
-
-
-
?
4-fluoroacetophenone + NADH + H+
1-(4-fluorophenyl)ethanol + NAD+
-
-
-
-
r
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
-
-
?
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
-
-
-
r
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
-
-
r
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
99% of the activity with (S)-(-)-1-phenylethanol
-
-
?
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
99% of the activity with (S)-1-phenylethanol
-
-
r
4-methyl-1-pentanol + NAD+
4-methyl-1-pentanal + NADH
-
-
-
-
?
4-methyl-1-pentanol + NAD+
4-methyl-1-pentanal + NADH
-
-
-
-
?
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzyl alcohol + NAD+
-
-
-
-
?
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzyl alcohol + NAD+
-
-
-
-
r
4-nitrotetrazolium violet + NADH + H+
a formazan + NAD+
p-NTF, the tetrazolium contacts with NADH on the enzyme surface without intermediate carriers (the zinc in the active ADH site is not a electron carrier) and accepts electrons. Alcohols are not able to reduce p-NTF
-
-
?
4-nitrotetrazolium violet + NADH + H+
a formazan + NAD+
p-NTF, the tetrazolium contacts with NADH on the enzyme surface without intermediate carriers (the zinc in the active ADH site is not a electron carrier) and accepts electrons. Alcohols are not able to reduce p-NTF
-
-
?
5-hydroxymethylfurfural + NADH + H+
(furan-2,5-diyl)dimethanol + NAD+
mutant enzyme S109P/L116S/Y294C
-
-
?
5-hydroxymethylfurfural + NADH + H+
(furan-2,5-diyl)dimethanol + NAD+
-
no substrate for wild-type, reaction is catalyzed by mutants Y295C and S110P/Y295C
-
-
?
5-hydroxymethylfurfural + NADH + H+
(furan-2,5-diyl)dimethanol + NAD+
-
no substrate for wild-type, reaction is catalyzed by mutants Y295C and S110P/Y295C
-
-
?
5alpha-androstan-17beta-ol-3-one + NADH + H+
3beta,17beta-dihydroxy-5alpha-androstan + NAD+
-
-
-
-
?
5alpha-androstan-17beta-ol-3-one + NADH + H+
3beta,17beta-dihydroxy-5alpha-androstan + NAD+
-
-
-
-
?
5beta-androstan-17beta-ol-3-one + NAD+
5beta-androstan-3,17-dione + NADH
-
-
-
-
?
5beta-androstan-17beta-ol-3-one + NAD+
5beta-androstan-3,17-dione + NADH
-
low activity
-
-
?
5beta-androstan-3beta-ol-17-one + NAD+
5beta-androstan-3,17-dione + NADH
-
-
-
-
?
5beta-androstan-3beta-ol-17-one + NAD+
5beta-androstan-3,17-dione + NADH
-
-
-
-
?
6-methoxy-2-naphthaldehyde + NADH + H+
(6-methoxynaphthalen-2-yl)methanol + NAD+
-
substrate for class II ADH
-
-
?
6-methoxy-2-naphthaldehyde + NADH + H+
(6-methoxynaphthalen-2-yl)methanol + NAD+
-
substrate for class II ADH
-
-
r
6-methoxy-2-naphthaldehyde + NADH + H+
(6-methoxynaphthalen-2-yl)methanol + NAD+
-
class II isozyme, reductive activity
-
-
?
7-cis-retinol + NAD+
7-cis-retinal + NADH
-
-
-
-
?
7-cis-retinol + NAD+
7-cis-retinal + NADH
-
-
-
-
?
9-cis-retinol + NAD+
9-cis-retinal + NADH
-
-
-
?
9-cis-retinol + NAD+
9-cis-retinal + NADH
-
-
-
-
?
9-cis-retinol + NAD+
9-cis-retinal + NADH
-
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
activity of the enzyme is confirmed by proteome analysis and enzyme assays with cell extract glycerol-grown cells
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
100% of the activity with butan-2-ol
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
83.2% of the activity with butan-1-ol
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
Megalodesulfovibrio gigas
-
the reduction of acetaldehyde is 4.9fold faster than the oxidation of ethanol
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
reduced by isoenzyme A2 and C2, no activity with isoenzyme B2
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
best substrate
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
best substrate
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
5.7% of the activity with glutaraldehyde
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
5.7% of the activity with glutaraldehyde
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
preferred substrates
-
-
ir
acetaldehyde + NADH + H+
ethanol + NAD+
-
cells with an extra copy of ADH1 display chronological life-span extension. Antioxidant enzymes are induced in 2xADH1 cells. Strains carrying an extra ADH1 copy show extended replicative life span and increased Sir2p activity
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
mutant enzyme S109P/L116S/Y294C
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
Sporotrichum pulverulentum
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
C7IV28
AdhE is a bifunctional enzyme, containing both aldehyde dehydrogenase and alcohol dehydrogenase activities
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
C7IV28
AdhE is a bifunctional enzyme, containing both aldehyde dehydrogenase and alcohol dehydrogenase activities
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
AdhE is a bifunctional enzyme, containing both aldehyde dehydrogenase and alcohol dehydrogenase activities
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
reduced at the highest rate of all aldehydes tested
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+ + H+
-
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+ + H+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
NADPH is not a cofactor for wild-type, but for mutant P704L/H734R
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
NADPH is not a cofactor for wild-type, but for mutant P704L/H734R
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
16% of the activity with NADH
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
?
acetone + NADH
isopropanol + NAD+
-
-
-
r
acetone + NADH
isopropanol + NAD+
-
-
-
r
acetone + NADH + H+
propan-2-ol + NAD+
-
-
-
-
?
acetone + NADH + H+
propan-2-ol + NAD+
-
-
-
?
acetone + NADH + H+
propan-2-ol + NAD+
-
1.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
acetone + NADH + H+
propan-2-ol + NAD+
-
-
-
-
?
acetone + NADH + H+
propan-2-ol + NAD+
-
-
-
?
acetone + NADH + H+
propan-2-ol + NAD+
-
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
1.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
40% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
r
alcohol + NAD+
aldehyde or ketone + NADH
-
medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes
-
-
r
alcohol + NAD+
aldehyde or ketone + NADH
-
medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes
-
-
r
all-trans-retinol + NAD+
all-trans-retinal + NADH
-
-
-
?
all-trans-retinol + NAD+
all-trans-retinal + NADH
-
-
-
-
?
all-trans-retinol + NAD+
all-trans-retinal + NADH
-
-
-
-
r
all-trans-retinol + NAD+
all-trans-retinal + NADH
-
ADH4 might be involved in biosynthesis of retinoic acid
-
-
r
all-trans-retinol + NAD+
all-trans-retinal + NADH
-
-
-
-
?
all-trans-retinol + NAD+
all-trans-retinal + NADH
-
-
-
-
?
allyl alcohol + NAD+
acrolein + NADH
-
-
-
-
?
allyl alcohol + NAD+
acrolein + NADH
-
-
product is toxic in mouse hepatocytes due to cell protein carbonylation following exposure to crotyl alcohol
-
?
allyl alcohol + NAD+
acrolein + NADH
-
-
-
-
r
allyl alcohol + NAD+
prop-2-en-1-al + NADH
-
-
-
-
?
allyl alcohol + NAD+
prop-2-en-1-al + NADH
-
-
-
-
?
allyl alcohol + NAD+
prop-2-en-1-al + NADH
-
no activity
-
-
?
allyl alcohol + NAD+
prop-2-en-1-al + NADH
-
-
-
-
?
allyl alcohol + NAD+
prop-2-en-1-al + NADH
-
-
-
-
?
allyl alcohol + NAD+
prop-2-en-1-al + NADH
-
-
-
-
?
alpha-tetralone + NADH + H+
(S)-alpha-tetralol + NAD+
-
-
99% enantiomeric excess
-
r
alpha-tetralone + NADH + H+
(S)-alpha-tetralol + NAD+
-
more than 99% enantiomeric excess
-
-
?
benzaldehyde + NADH
benzyl alcohol + NAD+
-
-
-
-
?
benzaldehyde + NADH
benzyl alcohol + NAD+
-
-
-
-
?
benzaldehyde + NADH
benzyl alcohol + NAD+
-
reduced by isoenzyme A2 and C2, no activity with isoenzyme B2
-
-
r
benzaldehyde + NADH
benzyl alcohol + NAD+
-
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
1.2% of the activity with acetaldehyde
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
21% of activity with N-benzyl-3-pyrrolidinone
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
93% of the activity with butan-2-ol
-
r
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
74% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
3% activity compared to benzyl alcohol
-
-
r
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
-
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
-
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
14% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
14% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
-
-
-
r
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH
-
oxidized by isoenzyme A2 and C2 no activity with isoenzyme B2
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
oxidation with isoenzyme ADH-1 and ADH-3, no activity with isoenzyme ADH-2
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
no activity
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
i.e. phenylmethanol
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
199% of the activity with 2-phenylethanol
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
33% of the activity with butan-2-ol
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
100% activity
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
178% activity compared to cyclohexanone
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
47% activity compared to cyclohexanol
154% activity compared to cyclohexanone
-
r
benzylacetone + NADH + H+
4-phenylbutan-2-ol + NAD+
-
activity is 1.3fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
benzylacetone + NADH + H+
4-phenylbutan-2-ol + NAD+
-
-
-
-
r
benzylalcohol + NAD+
benzaldehyde + NADH + H+
weak activity
-
-
r
benzylalcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
r
benzylalcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzylalcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
butan-1-ol + NAD+
butanal + NADH + H+
-
90.7% of the activity with ethanol
-
-
?
butan-1-ol + NAD+
butanal + NADH + H+
-
-
-
r
butan-1-ol + NAD+
butanal + NADH + H+
-
-
-
?
butan-1-ol + NAD+
butanal + NADH + H+
best substrate
-
-
r
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
(R)-2-butanol and (S)-2-butanol
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
Sporotrichum pulverulentum
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
no activity
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
weak activity
-
-
?
butan-2-one + NADH
butan-2-ol + NAD+
-
-
-
r
butan-2-one + NADH
butan-2-ol + NAD+
-
-
-
-
r
butanal + NADH
n-butanol + NAD+
-
-
-
-
?
butanal + NADH
n-butanol + NAD+
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
pH 10.0: oxidized by ADH-1 and ADH-3, no activity with isoenzyme ADH-2
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
weak
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
activity with ADH I, no activity with ADH II
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
oxidized by enzyme form ADH-I, no activity with enzyme form ADH-II
-
-
?
butanol + NAD+
butyraldehyde + NADH + H+
-
immobilized HLAD shows about 95% reaction rate and free HLAD shows about 90% reaction rate with butanol compared to ethanol at pH 8.8 and 30°C
-
-
?
butanol + NAD+
butyraldehyde + NADH + H+
-
-
-
-
r
butanol + NAD+
butyraldehyde + NADH + H+
-
359% activity compared to cyclohexanone
-
r
butyraldehyde + NADH
n-butanol + NAD+
-
-
-
-
r
butyraldehyde + NADH
n-butanol + NAD+
Megalodesulfovibrio gigas
-
-
-
-
r
butyraldehyde + NADH
n-butanol + NAD+
-
activity with enzyme form ADH I, no activity with enzyme form ADH II
-
r
butyraldehyde + NADH + H+
n-butanol + NAD+
-
best substrate for isozyme ADH II
-
-
r
butyraldehyde + NADH + H+
n-butanol + NAD+
-
best substrate for isozyme ADH II
-
-
r
butyraldehyde + NADH + H+
n-butanol + NAD+
-
-
-
-
?
butyraldehyde + NADH + H+
n-butanol + NAD+
-
-
-
-
?
capronaldehyde + NADH
1-hexanol + NAD+
-
best substrate
-
-
r
capronaldehyde + NADH
1-hexanol + NAD+
-
best substrate
-
-
r
capronaldehyde + NADH
1-hexanol + NAD+
-
best substrate
-
-
r
chloroacetone + NADH + H+
1-chloropropan-2-ol + NAD+
-
41.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
chloroacetone + NADH + H+
1-chloropropan-2-ol + NAD+
-
238% of the activity with phenyl trifluoromethyl ketone
-
-
?
cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
-
-
-
-
r
cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
-
-
-
r
cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
-
-
-
r
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH
-
-
-
-
?
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH
-
-
-
-
?
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH
-
-
-
-
?
crotyl alcohol + NAD+
crotonaldehyde + NADH
-
-
-
-
?
crotyl alcohol + NAD+
crotonaldehyde + NADH
-
product is toxic in mouse hepatocytes due to cell protein carbonylation following exposure to crotyl alcohol
-
-
?
crotyl alcohol + NAD+
crotonaldehyde + NADH
-
-
product is toxic in mouse hepatocytes due to cell protein carbonylation following exposure to crotyl alcohol
-
?
crotyl alcohol + NAD+
crotonaldehyde + NADH
-
-
-
-
?
cycloheptanol + NAD+
cycloheptanone + NADH + H+
-
-
-
?
cycloheptanol + NAD+
cycloheptanone + NADH + H+
-
-
-
r
cycloheptanol + NAD+
cycloheptanone + NADH + H+
37% of activity compared to (S)-1-indanol
-
-
?
cycloheptanone + NADH
cycloheptanol + NAD+
-
-
-
-
?
cycloheptanone + NADH
cycloheptanol + NAD+
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
reaction is catalyzed by the cathodic pyrazole-sensitive enzyme, no activity with the cathodic pyrazole-insensitive enzyme and by the anodic pyrazole-insensitive enzyme
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
oxidized by isoenzyme A2, no activity with isoenzymes B2 and C2
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
no activity
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH + H+
12% of the activity with 2,3-butanediol
-
-
r
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
-
-
r
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH + H+
52% of the activity with 1-phenylethanol
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH + H+
100% activity
100% activity
-
r
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
13% of the activity with (S)-(-)-1-phenylethanol
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
13% of the activity with (S)-(-)-1-phenylethanol
-
-
?
cyclohexanone + NADH
cyclohexanol + NAD+
-
-
-
-
?
cyclohexanone + NADH
cyclohexanol + NAD+
-
best cyclic ketone substrate
-
-
?
cyclohexanone + NADH + H+
cyclohexanol + NAD+
-
-
-
-
?
cyclohexanone + NADH + H+
cyclohexanol + NAD+
38% of the activity with acetoin
-
-
r
cyclohexanone + NADH + H+
cyclohexanol + NAD+
-
-
-
?
cyclohexanone + NADH + H+
cyclohexanol + NAD+
-
-
-
?
cyclopentanol + NAD+
? + NADH
-
-
-
-
?
cyclopentanol + NAD+
? + NADH
-
-
-
-
?
cyclopentanol + NAD+
cyclopentanone + NADH + H+
-
92% of the activity with butan-2-ol
-
?
cyclopentanol + NAD+
cyclopentanone + NADH + H+
55% of the activity compared to isoborneol
-
-
?
D-glucitol + NAD+
? + NADH
-
-
-
-
?
D-glucitol + NAD+
? + NADH
-
-
-
-
?
diacetyl + NADH + H+
?
-
16.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
diacetyl + NADH + H+
?
-
16.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
acetaldehyde is the best substrate for isozyme ADH I
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
acetaldehyde is the best substrate for isozyme ADH I
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
76% of the activity with 2-phenylethanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
76% of the activity with 2-phenylethanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
the reduction of acetaldehyde of ADH-MII is about 7times higher than that of the oxidation of ethanol
-
r
ethanol + NAD+
acetaldehyde + NADH
-
the reduction of acetaldehyde of ADH-MII is about 7times higher than that of the oxidation of ethanol
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
no cooperativity between the 2 active sites of the enzyme
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
plays an important role in the metabolism of ethanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH
isomerization of the enzyme-NAD+ complex is the rate-limiting step for acetaldehyde reduction by the wild-type enzyme
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
proton and hydride equivalent transfer in the alcohol dehydrogenase enzymatic reaction are modulated by the correlated motions between NAD+ and the cofactor domain
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
285565, 285567, 285568, 285569, 285572, 285573, 285575, 285608, 285651, 655197, 655206, 655479 -
-
?
ethanol + NAD+
acetaldehyde + NADH
-
class III isoenzyme chi-ADH oxidizes ethanol very poorly
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
no oxidation with class III enzyme
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
chi-ADH plays an important role in the metabolism of long chain alcohols and aldehydes
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
the anodic enzyme form may contribute significantly to alcohol elimination in man, particularly at high concentrations when the other enzyme species are saturated
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
the enzyme plays a significant role in first-pass metabolism of ethanol in human
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
enzyme is responsible for the major ethanol oxidation capacity in the body. The products acetaldehyde and NADH are responsible for the most of the toxic effects and metabolic disturbances produced by ethanol ingestion
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
Megalodesulfovibrio gigas
-
the reduction of acetaldehyde is 4.9fold faster than the oxidation of ethanol
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
no activity with isoenzyme B2, oxidized by isoenzyme A2 and C2
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
role of the major liver isoenzyme A2 in ethanol metabolism
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
DH3 plays an important role in systemic ethanol metabolism at higher levels of blood ethanol through activation by cytoplasmic solution hydrophobicity
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
isoenzyme ADH-1 and ADH-3, no activity with isoenzyme ADH-2
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
rate-limiting step of the alcoholic fermentation
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
Sporotrichum pulverulentum
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
best substrate
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
63% activity compared to 1-propanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
63% activity compared to 1-propanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
no activity with NADP+, in reverse reaction no activity with NADPH
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
83% of the activity with butan-2-ol
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
ethanol is the best substrate
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
substrate for isozyme ADH1C, extremely poor substrate for isozyme ADH3
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
substrate for isozyme ADH2
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
substrate for isozyme ADH4
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
12% of the activity with butan-1-ol
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
the enzyme is highly specific for ethanol with NAD+ as the coenzyme
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
best substrate
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
33% of the activity with 1,4-butanediol
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
100% activity
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
100% activity
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
88% activity compared to cyclohexanol
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
8.5% of the activity with ethanol and NAD+
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethyl 3-oxobutanoate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
-
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
-
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
309% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADH + H+
(S)-ethyl-4-chloro-3-hydroxybutanoate + NAD+
-
COBE, ADH performs a enantioselective reduction, reacting as an anti-Prelog reductase. An enantiomeric excess of 99% is achieved in the process
-
-
r
ethyl 4-chloro-3-oxobutanoate + NADH + H+
(S)-ethyl-4-chloro-3-hydroxybutanoate + NAD+
COBE, ADH performs a enantioselective reduction, reacting as an anti-Prelog reductase
-
-
r
ethyl 4-chloro-3-oxobutanoate + NADH + H+
(S)-ethyl-4-chloro-3-hydroxybutanoate + NAD+
COBE, ADH performs a enantioselective reduction, reacting as an anti-Prelog reductase
-
-
r
ethyl 4-chloro-3-oxobutanoate + NADH + H+
(S)-ethyl-4-chloro-3-hydroxybutanoate + NAD+
-
COBE, ADH performs a enantioselective reduction, reacting as an anti-Prelog reductase
-
-
r
ethyl 4-chloro-3-oxobutanoate + NADH + H+
(S)-ethyl-4-chloro-3-hydroxybutanoate + NAD+
COBE, ADH performs a enantioselective reduction, reacting as an anti-Prelog reductase
-
-
r
ethyl 4-chloro-3-oxobutanoate + NADH + H+
(S)-ethyl-4-chloro-3-hydroxybutanoate + NAD+
COBE, ADH performs a enantioselective reduction, reacting as an anti-Prelog reductase
-
-
r
ethyl benzoylformate + NADH + H+
ethyl (R)-mandelate + NAD+
-
50% enantiomeric excess
-
r
ethyl benzoylformate + NADH + H+
ethyl (R)-mandelate + NAD+
-
50% enantiomeric excess
-
r
ethyl pyruvate + NADH
?
about 15% of the activity compared to isatin
-
-
?
ethyl pyruvate + NADH
?
about 15% of the activity compared to isatin
-
-
?
ethylene glycol + NAD+
?
-
poor substrate
-
-
r
ethylene glycol + NAD+
?
-
poor substrate
-
-
r
ethylene glycol + NAD+
? + NADH
-
no activity
-
-
?
ethylene glycol + NAD+
? + NADH
-
-
-
-
?
ethylene glycol + NAD+
? + NADH
-
class III isoenzyme chi-ADH shows no activity
-
-
?
ethylene glycol + NAD+
? + NADH
-
-
-
-
?
ethylene glycol + NAD+
? + NADH
-
-
-
-
?
ethylene glycol + NAD+
? + NADH
-
oxidized by isoenzyme A2, no activity with isoenzyme B2 and C2
-
-
?
formaldehyde + NADH + H+
methanol + NAD+
-
-
-
r
formaldehyde + NADH + H+
methanol + NAD+
-
-
-
r
formaldehyde + NADH + H+
methanol + NAD+
Megalodesulfovibrio gigas
-
-
-
-
?
formaldehyde + NADH + H+
methanol + NAD+
-
reduced by isoenzyme A2, no activity with isoenzyme B2 and C2
-
-
r
formaldehyde + NADH + H+
methanol + NAD+
-
-
-
-
?
formaldehyde + NADH + H+
methanol + NAD+
-
-
-
-
?
formaldehyde + NADH + H+
methanol + NAD+
-
activity with ADH I, no activity with ADH II
-
-
?
furfural + NADH + H+
furfuryl alcohol + NAD+
-
-
-
-
?
furfural + NADH + H+
furfuryl alcohol + NAD+
low activity
-
-
ir
furfural + NADH + H+
furfuryl alcohol + NAD+
mutant enzyme S109P/L116S/Y294C
-
-
?
furfural + NADH + H+
furfuryl alcohol + NAD+
-
-
-
-
?
furfural + NADH + H+
furfuryl alcohol + NAD+
-
-
-
-
?
furfural + NADH + H+
furfuryl alcohol + NAD+
-
activity with enzyme form ADH I, no activity with enzyme form ADH II
-
r
furfuryl alcohol + NAD+
furfural + NADH
-
-
-
-
?
furfuryl alcohol + NAD+
furfural + NADH
-
activity with ADH I, no activity with ADH II
-
r
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
-
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
-
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
-
-
r
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
-
-
r
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
-
-
-
?
glycolaldehyde + NADH + H+
glycol + NAD+
-
-
-
-
?
glycolaldehyde + NADH + H+
glycol + NAD+
low activity
-
-
ir
glycolaldehyde + NADH + H+
glycol + NAD+
-
-
-
-
?
hexan-1-ol + NAD+
hexanal + NADH + H+
-
-
-
r
hexan-1-ol + NAD+
hexanal + NADH + H+
77.1% of the activity with butan-1-ol
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexanal + NADH + H+
n-hexanol + NAD+
-
49% of the activity with acetaldehyde
-
-
?
hexanal + NADH + H+
n-hexanol + NAD+
-
22.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
Sporotrichum pulverulentum
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
activity with ADH I, no activity with ADH II
-
-
?
isatin + NADH + H+
? + NAD+
-
-
-
r
isatin + NADH + H+
? + NAD+
-
-
-
r
iso-propanol + NAD+
isopropanal + NADH + H+
-
-
-
?
iso-propanol + NAD+
isopropanal + NADH + H+
-
-
-
?
isobutyraldehyde + NADH + H+
isobutanol + NAD+
-
-
-
r
isobutyraldehyde + NADH + H+
isobutanol + NAD+
-
-
-
-
?
isobutyraldehyde + NADH + H+
isobutanol + NAD+
-
-
-
-
?
isopentanol + NAD+
? + NADH + H+
low affinity
-
-
?
isopentanol + NAD+
? + NADH + H+
low affinity
-
-
?
isopropanol + NAD+
acetone + NADH + H+
low affinity
-
-
?
isopropanol + NAD+
acetone + NADH + H+
low affinity
-
-
?
isopropanol + NAD+
acetone + NADH + H+
-
-
-
-
?
m-nitrobenzaldehyde + NADH + H+
m-nitrobenzyl alcohol + NAD+
-
substrate of class IV ADH isozyme
-
-
r
m-nitrobenzaldehyde + NADH + H+
m-nitrobenzyl alcohol + NAD+
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
16% activity compared to 1-propanol
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
16% activity compared to 1-propanol
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
reaction is extremly weak
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
reaction is extremly weak
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
ir
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
anodic enzyme form shows no activity
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
class I isoenzymes
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity with isoenzyme beta3,beta3
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
activity detected with class II isoenzyme pi-ADH
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
oxidized by isoenzyme beta1,beta1
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
class III isoenzyme chi-ADH shows no activity
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity with class III enzyme
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
reaction is catalyzed by the cathodic pyrazole-sensitive enzyme, no activity by the cathodic pyrazole-insensitive enzyme and by the anodic pyrazole-insensitive enzyme
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
weak activity with isoenzyme A2, no activity with isoenzyme B2 and C2
-
-
?, r
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity at pH 7.5, slight activity at pH 10.8
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
oxidized with ADH-3, no activity with ADH-1 and ADH-2
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
reaction is catalyzed by the pyrazole-sensitive enzyme, no activity with the pyrazole-insensitive enzyme
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
Sporotrichum pulverulentum
-
no activity
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
low activity
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
49.9% activity compared to ethanol
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
?
methyl 3-oxobutanoate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
-
-
-
?
methyl 3-oxobutanoate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
-
-
?
methyl 3-oxobutanoate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
-
-
?
methyl 3-oxobutanoate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
-
-
-
?
methyl 3-oxobutanoate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
-
-
?
methyl 3-oxobutanoate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
-
-
?
methyl 3-oxobutanoate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
-
-
?
methyl 3-oxobutanoate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
130% of the activity with phenyl trifluoromethyl ketone
-
-
?
methyl 4-chloro-3-oxobutanoate + NADH + H+
methyl 4-chloro-3-hydroxybutanoate + NAD+
-
-
-
-
?
methyl 4-chloro-3-oxobutanoate + NADH + H+
methyl 4-chloro-3-hydroxybutanoate + NAD+
-
-
-
?
methyl 4-chloro-3-oxobutanoate + NADH + H+
methyl 4-chloro-3-hydroxybutanoate + NAD+
-
-
-
?
methyl 4-chloro-3-oxobutanoate + NADH + H+
methyl 4-chloro-3-hydroxybutanoate + NAD+
-
-
-
-
?
methyl 4-chloro-3-oxobutanoate + NADH + H+
methyl 4-chloro-3-hydroxybutanoate + NAD+
-
-
-
?
methyl 4-chloro-3-oxobutanoate + NADH + H+
methyl 4-chloro-3-hydroxybutanoate + NAD+
-
-
-
?
methyl benzoylformate + NADH
?
about 35% of the activity compared to isatin
-
-
?
methyl benzoylformate + NADH
?
about 35% of the activity compared to isatin
-
-
?
N-benzyl-3-pyrrolidinone + NADH + H+
(S)-N-benzyl-3-pyrrolidinol + NAD+
-
-
-
-
r
N-benzyl-3-pyrrolidinone + NADH + H+
(S)-N-benzyl-3-pyrrolidinol + NAD+
-
(S)-N-benzyl-3-pyrrolidinol is produced at an enantiomeric excess of more than 99.9%
-
-
r
n-butanol + NAD+
butylaldehyde + NADH + H+
-
-
-
-
?
n-butanol + NAD+
butylaldehyde + NADH + H+
-
-
-
-
r
n-butanol + NAD+
n-butanal + NADH
-
-
-
-
?
n-butanol + NAD+
n-butanal + NADH
-
-
-
-
r
n-propanol + NAD+
propanal + NADH
-
-
-
?
n-propanol + NAD+
propanal + NADH
-
-
-
-
?
n-propanol + NAD+
propanal + NADH + H+
-
-
-
-
r
n-propanol + NAD+
propanal + NADH + H+
-
-
-
?
n-propanol + NAD+
propanal + NADH + H+
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
Sporotrichum pulverulentum
-
weak activity
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
activity with ADH I, no activity with ADH II
-
-
?
octan-2-ol + NAD+
octan-2-one + NADH
-
(R)-2-octanol and (S)-2-octanol
-
-
?
octan-2-ol + NAD+
octan-2-one + NADH
-
-
-
-
?
octan-2-ol + NAD+
octan-2-one + NADH
-
-
-
-
?
octan-2-ol + NAD+
octan-2-one + NADH
Sporotrichum pulverulentum
-
weak activity
-
-
?
octanal + NADH + H+
octanol + NAD+
-
-
-
-
?
octanal + NADH + H+
octanol + NAD+
-
-
-
-
?
octanal + NADH + H+
octanol + NAD+
-
-
-
-
?
octanol + NAD+
octanal + NADH
-
-
-
-
?
octanol + NAD+
octanal + NADH
-
-
-
-
?
octanol + NAD+
octanal + NADH
-
-
-
?
octanol + NAD+
octanal + NADH + H+
-
-
-
?
octanol + NAD+
octanal + NADH + H+
-
immobilized and free HLAD show 100% reaction rate with octanol compared to ethanol at pH 8.8 and 30°C
-
-
?
octanol + NAD+
octanal + NADH + H+
-
-
-
-
?
octanol + NAD+
octanal + NADH + H+
-
-
-
?
octanol + NAD+
octanal + NADH + H+
-
-
-
r
octanol + NAD+
octanal + NADH + H+
-
178% activity compared to cyclohexanone
-
r
p-methoxybenzyl alcohol + NAD+
p-methoxybenzaldehyde + NADH + H+
-
-
-
-
r
p-methoxybenzyl alcohol + NAD+
p-methoxybenzaldehyde + NADH + H+
-
-
-
r
pentan-1-ol + NAD+
pentanal + NADH + H+
-
75.6% of the activity with ethanol
-
-
?
pentan-1-ol + NAD+
pentanal + NADH + H+
-
76% of the activity with butan-2-ol
-
?
pentan-2-ol + NAD+
2-pentanone + NADH
-
(R)-2-pentanol and (S)-2-pentanol
-
r
pentan-2-ol + NAD+
2-pentanone + NADH
-
-
-
-
?
pentan-2-ol + NAD+
2-pentanone + NADH
-
-
-
-
?
pentan-2-ol + NAD+
2-pentanone + NADH
-
-
-
-
?
pentan-2-ol + NAD+
2-pentanone + NADH
Sporotrichum pulverulentum
-
-
-
-
?
pentan-2-ol + NAD+
pentan-2-one + NADH + H+
-
93% of the activity with butan-2-ol
-
?
pentan-2-ol + NAD+
pentan-2-one + NADH + H+
-
9% of the activity with 2,3-butanediol
-
?
pentan-3-ol + NAD+
3-pentanone + NADH
-
-
-
r
pentan-3-ol + NAD+
3-pentanone + NADH
-
-
-
-
?
pentanal + NAD+
pentanone + NADH
-
-
-
-
?
pentanal + NAD+
pentanone + NADH
-
-
-
-
?
pentanal + NAD+
pentanone + NADH
-
-
-
-
?
pentanal + NAD+
pentanone + NADH
-
oxidized by isoenzyme A2 and B2, no activity with isoenzyme C2
-
-
?
pentanal + NAD+
pentanone + NADH
-
-
-
-
?
pentanal + NAD+
pentanone + NADH
Sporotrichum pulverulentum
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
activity with ADH I, no activity with ADH II
-
-
?
pentanol + NAD+
pentanal + NADH + H+
about 10% of the activity with ethanol or 1-propanol
-
-
?
pentanol + NAD+
pentanal + NADH + H+
about 10% of the activity with ethanol or 1-propanol
-
-
?
pentanol + NAD+
pentanal + NADH + H+
-
-
-
-
?
pentanol + NAD+
pentanal + NADH + H+
-
-
-
?
phenylacetaldehyde + NADH
phenylethanol + NAD+
-
-
-
-
r
phenylacetaldehyde + NADH
phenylethanol + NAD+
-
-
-
-
r
phenylacetaldehyde + NADH
phenylethanol + NAD+
-
-
-
-
r
phenylethanol + NAD+
phenylacetaldehyde + NADH + H+
-
15% of activity with N-benzyl-3-pyrrolidinol
-
-
?
phenylethanol + NAD+
phenylacetaldehyde + NADH + H+
-
-
-
r
phenylethanol + NAD+
phenylacetaldehyde + NADH + H+
-
-
-
r
primary or secondary alcohol + NAD+
aldehyde or ketone + NADH
-
-
-
-
r
primary or secondary alcohol + NAD+
aldehyde or ketone + NADH
-
-
-
-
r
propan-1,2-diol + NAD+
? + NADH
-
-
-
-
?
propan-1,2-diol + NAD+
? + NADH
Megalodesulfovibrio gigas
-
-
-
-
?
propan-1-ol + NAD+
propanal + NADH + H+
-
89.3% of the activity with ethanol
-
-
?
propan-1-ol + NAD+
propanal + NADH + H+
-
68% of the activity with butan-2-ol
-
r
propan-1-ol + NAD+
propanal + NADH + H+
64.3% of the activity with butan-1-ol
-
-
?
propan-1-ol + NAD+
propanal + NADH + H+
55% of the activity with butan-1-ol
-
-
r
propan-1-ol + NAD+
propanal + NADH + H+
-
-
-
?
propan-1-ol + NAD+
propanal + NADH + H+
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
Megalodesulfovibrio gigas
-
3% of the activity with ethanol
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
no activity
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
Sporotrichum pulverulentum
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
very low activity
-
-
?
propan-2-ol + NAD+
acetone + NADH + H+
-
79% of the activity with butan-2-ol
-
?
propan-2-ol + NAD+
acetone + NADH + H+
-
-
synthesis of [4R-(2)H]NADH with high yield by enzymatic oxidation of 2-propanol-d(8)
-
?
propan-2-ol + NAD+
acetone + NADH + H+
65% of the activity with butan-1-ol
-
-
r
propan-2-ol + NAD+
acetone + NADH + H+
-
6% activity compared to cyclohexanone
-
r
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
Megalodesulfovibrio gigas
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
Sporotrichum pulverulentum
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
-
?
propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
?
propionaldehyde + NADH
propanol + NAD+
-
-
-
-
?
propionaldehyde + NADH
propanol + NAD+
Megalodesulfovibrio gigas
-
-
-
-
?
propionaldehyde + NADH
propanol + NAD+
-
94% of the activity with acetaldehyde
-
-
?
propionaldehyde + NADH
propanol + NAD+
-
-
-
-
?
sinapaldehyde + NADH + H+
sinalcohol + NAD+
preferred substrates
-
-
r
sinapaldehyde + NADH + H+
sinalcohol + NAD+
preferred substrates
-
-
r
trans-cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
-
% of the activity with phenyl trifluoromethyl ketone
-
-
?
trans-cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
-
-
-
r
ubiquinone + NADH + H+
ubiquinol + NAD+
-
-
-
?
ubiquinone + NADH + H+
ubiquinol + NAD+
-
-
-
?
valeraldehyde + NADH
1-pentanol + NAD+
-
-
-
-
r
valeraldehyde + NADH
1-pentanol + NAD+
-
-
-
-
r
valeraldehyde + NADH
1-pentanol + NAD+
-
-
-
-
r
additional information
?
-
-
the NADH-dependent alcohol dehydrogenases from Acetobacter aceti with anti-Prelog stereoselectivity exhibits high specific activity on beta-ketoesters, such as methyl or ethyl 3-oxobutanoate, with maximum specific activity (66 U/mg) on ethyl 4-chloro-3-oxobutanoate. Development of biotransformation process for asymmetric reduction with anti-Prelog NADH-dependent alcohol dehydrogenases. Product identification using gas chromatography
-
-
-
additional information
?
-
-
substrate specificity of the 2 isozmyes with various substrates, overview, isozymes are highly specific for the (R)-stereoisomers and enantioselctive for the R(-)isomers
-
-
?
additional information
?
-
-
substrate specificity of the 2 isozmyes with various substrates, overview, isozymes are highly specific for the (R)-stereoisomers and enantioselctive for the R(-)isomers
-
-
?
additional information
?
-
development of biotransformation process for asymmetric reduction with anti-Prelog NADH-dependent alcohol dehydrogenases. Product identification using gas chromatography
-
-
-
additional information
?
-
the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone
-
-
?
additional information
?
-
the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone
-
-
?
additional information
?
-
development of biotransformation process for asymmetric reduction with anti-Prelog NADH-dependent alcohol dehydrogenases. Product identification using gas chromatography
-
-
-
additional information
?
-
-
no activity with isopropanol, 2,3-butanediol, isobutanol, isoamyl alcohol, cyclohexanol, acetone, cyclohexanone, acetophenone and alpha-tetralone
-
-
-
additional information
?
-
-
no activity with isopropanol, 2,3-butanediol, isobutanol, isoamyl alcohol, cyclohexanol, acetone, cyclohexanone, acetophenone and alpha-tetralone
-
-
-
additional information
?
-
Candida albicans ADH1 is a bifunctional enzyme that catalyzes methylglyoxal oxidation and reduction, cf. EC 1.2.1.23
-
-
?
additional information
?
-
-
Candida albicans ADH1 is a bifunctional enzyme that catalyzes methylglyoxal oxidation and reduction, cf. EC 1.2.1.23
-
-
?
additional information
?
-
-
one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII
-
-
?
additional information
?
-
-
one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII
-
-
?
additional information
?
-
-
the enzyme exhibits broad substrate specificity towards aliphatic ketones, cycloalkanones, aromatic ketones, and ketoesters
-
-
?
additional information
?
-
-
the enzyme exhibits broad substrate specificity towards aliphatic ketones, cycloalkanones, aromatic ketones, and ketoesters
-
-
?
additional information
?
-
the bifunctional enzymes commonly produce ethanol from acetyl-CoA with acetaldehyde as intermediate
-
-
-
additional information
?
-
-
the bifunctional enzymes commonly produce ethanol from acetyl-CoA with acetaldehyde as intermediate
-
-
-
additional information
?
-
ADHE catalyzes the reversible NADH-mediated interconversions of acetyl-CoA, acetaldehyde, and ethanol but seemed to be poised toward the production of ethanol from acetaldehyde
-
-
-
additional information
?
-
-
ADHE catalyzes the reversible NADH-mediated interconversions of acetyl-CoA, acetaldehyde, and ethanol but seemed to be poised toward the production of ethanol from acetaldehyde
-
-
-
additional information
?
-
the bifunctional enzymes commonly produce ethanol from acetyl-CoA with acetaldehyde as intermediate
-
-
-
additional information
?
-
ADHE catalyzes the reversible NADH-mediated interconversions of acetyl-CoA, acetaldehyde, and ethanol but seemed to be poised toward the production of ethanol from acetaldehyde
-
-
-
additional information
?
-
-
bifunctional enzyme consisting of an N-terminal acetaldehyde dehydrogenase (ALDH) and a C-terminal alcohol dehydrogenase (ADH). The specificity constant (kcat/Km) is 47fold higher for acetaldehyde reductase than that for ethanol dehydrogenase
-
-
?
additional information
?
-
-
no activity with methanol, 2-propanol, and isoamyl alcohol
-
-
?
additional information
?
-
-
Cm-ADH2 cannot reduce branched aldehydes
-
-
?
additional information
?
-
-
when NADH is replaced with NADPH, the reaction rate is reduced by 0.6%
-
-
?
additional information
?
-
-
when NADH is replaced with NADPH, the reaction rate is reduced by 0.6%
-
-
?
additional information
?
-
-
activity is severely reduced towards aliphatic alcohols of more than 8 carbon atoms for the free enzyme, but not so with immobilized HLAD, exhibiting an activity towards C22 and C24 aliphatic alcohols higher than 50% of the highest value, obtained with C8
-
-
?
additional information
?
-
-
acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety. There is no detectable reduction of acycloNAD+ by secondary alcohols although these alcohols serve as competitive inhibitors. AcycloNAD+ converts horse liver ADH from a broad spectrum alcohol dehydrogenase, capable of utilizing either primary or secondary alcohols, into an exclusively primary alcohol dehydrogenase
-
-
?
additional information
?
-
horse liver alcohol dehydrogenase together with the NADH oxidase from Streptococcus mutans are applied for the oxidative lactamization of various amino alcohols, direct synthesis of lactams (5-, 6-, and 7-membered) starting from amino-alcohols in a bienzymatic cascade. Poor activity with aromatic amino alcohols (2-(2-aminoethyl)phenyl)-methanol and (2-(aminomethyl)phenyl) methanol
-
-
-
additional information
?
-
the bifunctional enzymes commonly produce ethanol from acetyl-CoA with acetaldehyde as intermediate
-
-
-
additional information
?
-
-
the bifunctional enzymes commonly produce ethanol from acetyl-CoA with acetaldehyde as intermediate
-
-
-
additional information
?
-
no activity is detected using 1 mM NADP+
-
-
?
additional information
?
-
-
no activity is detected using 1 mM NADP+
-
-
?
additional information
?
-
no activity is detected using 1 mM NADP+
-
-
?
additional information
?
-
no activity with methanol
-
-
?
additional information
?
-
-
no activity with methanol
-
-
?
additional information
?
-
no activity with methanol
-
-
?
additional information
?
-
-
no activity with methanol
-
-
?
additional information
?
-
-
broad substrate specificity
-
-
?
additional information
?
-
mutation at the substrate-binding site, or at a dimer interface, alters kinetic properties and protein oligomeric structure, active site flexibility is correlated with subunit interactions 20 A away
-
-
?
additional information
?
-
-
development of biotransformation process for asymmetric reduction with anti-Prelog NADH-dependent alcohol dehydrogenases. Product identification using gas chromatography
-
-
-
additional information
?
-
the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol
-
-
?
additional information
?
-
-
the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol
-
-
?
additional information
?
-
the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol
-
-
?
additional information
?
-
-
differences in the activities of total ADH and class I ADH isoenzyme between cancer liver tissues and healthy hepatocytes may be a factor in ethanol metabolism disorders, which can intensify carcinogenesis
-
-
?
additional information
?
-
1-octanal is no substrate for isozyme ADH1C
-
-
?
additional information
?
-
1-octanal is no substrate for isozyme ADH1C
-
-
?
additional information
?
-
-
1-octanal is no substrate for isozyme ADH1C
-
-
?
additional information
?
-
1-octanal is no substrate for isozyme ADH2
-
-
?
additional information
?
-
1-octanal is no substrate for isozyme ADH2
-
-
?
additional information
?
-
-
1-octanal is no substrate for isozyme ADH2
-
-
?
additional information
?
-
1-octanal is no substrate for isozyme ADH4
-
-
?
additional information
?
-
1-octanal is no substrate for isozyme ADH4
-
-
?
additional information
?
-
-
1-octanal is no substrate for isozyme ADH4
-
-
?
additional information
?
-
enzyme is an alcohol dehydrogenase with additional activity for all-trans-retinol, reaction of EC 1.1.1.184
-
-
?
additional information
?
-
-
enzyme is an alcohol dehydrogenase with additional activity for all-trans-retinol, reaction of EC 1.1.1.184
-
-
?
additional information
?
-
development of biotransformation process for asymmetric reduction with anti-Prelog NADH-dependent alcohol dehydrogenases. Product identification using gas chromatography
-
-
-
additional information
?
-
development of biotransformation process for asymmetric reduction with anti-Prelog NADH-dependent alcohol dehydrogenases. Product identification using gas chromatography
-
-
-
additional information
?
-
development of biotransformation process for asymmetric reduction with anti-Prelog NADH-dependent alcohol dehydrogenases. Product identification using gas chromatography
-
-
-
additional information
?
-
-
the enzyme does not act on short-chain normal alkyl alcohols, including methanol and ethanol
-
-
?
additional information
?
-
-
the enzyme does not act on short-chain normal alkyl alcohols, including methanol and ethanol
-
-
?
additional information
?
-
-
no activity detected with: N-benzyl-2-pyrrolidinone, 2-pyrrolidinone, 3-hexanone, 4-hydroxy-2-butanone, (R)-N-benzyl-3-pyrrolidinol, ethanol, 1,3-propanediol, 1-butanol, 1,4-butanediol, 1,2,3-butanetriol, 1,2,4-butanetriol, acetol, 2-phenyl-1-propanol, 3-phenyl-1-propanol, benzyl alcohol and glycerol. No activity with NADP+ or NADPH
-
-
?
additional information
?
-
-
alcohol dehydrogenase activity may not limit alcohol supply for ester production during ripening
-
-
?
additional information
?
-
-
involvement in the development of male hamster reproductive system
-
-
?
additional information
?
-
mutation at the substrate-binding site, or at a dimer interface, alters kinetic properties and protein oligomeric structure, active site flexibility is correlated with subunit interactions 20 A away
-
-
?
additional information
?
-
mutation at the substrate-binding site, or at a dimer interface, alters kinetic properties and protein oligomeric structure, active site flexibility is correlated with subunit interactions 20 A away
-
-
?
additional information
?
-
enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities
-
-
?
additional information
?
-
-
enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities
-
-
?
additional information
?
-
-
ADH1 preferrs primary alcohols containing C3-C8 carbons to secondary alcohols such as 2-propanol and 2-butanol. ADH1 possesses specific carboxylate ester-forming activity
-
-
?
additional information
?
-
ADH1 preferrs primary alcohols containing C3-C8 carbons to secondary alcohols such as 2-propanol and 2-butanol. ADH1 possesses specific carboxylate ester-forming activity
-
-
?
additional information
?
-
ADH1 preferrs primary alcohols containing C3-C8 carbons to secondary alcohols such as 2-propanol and 2-butanol. ADH1 possesses specific carboxylate ester-forming activity
-
-
?
additional information
?
-
-
no activity with methanol, 1-butanol, glycerol or 2-propanol
-
-
?
additional information
?
-
-
no activity with methanol, 1-butanol, glycerol or 2-propanol
-
-
?
additional information
?
-
methanol, formaldehyde, and acetone are no substrates for HpADH3
-
-
?
additional information
?
-
-
methanol, formaldehyde, and acetone are no substrates for HpADH3
-
-
?
additional information
?
-
methanol, formaldehyde, and acetone are no substrates for HpADH3
-
-
?
additional information
?
-
-
enzyme may be involved in the metabolism of dietary wax esters in salmonid fish
-
-
?
additional information
?
-
no activity towards methanol
-
-
?
additional information
?
-
-
no activity towards methanol
-
-
?
additional information
?
-
no activity towards methanol
-
-
?
additional information
?
-
-
the enzyme additionally catalyzes selective reduction of 3-quinuclidinone to (R)-3-quinuclidinol, with 84% ee and 62% conversion after 22 h
-
-
?
additional information
?
-
no activity towards methanol, ethanol, 1-propanol, triethylene glycol, polyethylene glycol 400, polyethylene glycol 1000, D-sorbitol, D-sorbose, formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and valeraldehyde
-
-
?
additional information
?
-
-
no activity towards methanol, ethanol, 1-propanol, triethylene glycol, polyethylene glycol 400, polyethylene glycol 1000, D-sorbitol, D-sorbose, formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and valeraldehyde
-
-
?
additional information
?
-
no activity towards methanol, ethanol, 1-propanol, triethylene glycol, polyethylene glycol 400, polyethylene glycol 1000, D-sorbitol, D-sorbose, formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and valeraldehyde
-
-
?
additional information
?
-
the enzyme catalyzes NAD(H)-dependent oxidation of various alcohols and reduction of aldehydes, with a marked preference for substrates with functional group at the terminal carbon atom
-
-
?
additional information
?
-
-
the enzyme catalyzes NAD(H)-dependent oxidation of various alcohols and reduction of aldehydes, with a marked preference for substrates with functional group at the terminal carbon atom
-
-
?
additional information
?
-
the enzyme catalyzes NAD(H)-dependent oxidation of various alcohols and reduction of aldehydes, with a marked preference for substrates with functional group at the terminal carbon atom
-
-
?
additional information
?
-
broad substrate specificity with a preference for the reduction of ketones and the oxidation of secondary alcohols
-
-
?
additional information
?
-
-
broad substrate specificity with a preference for the reduction of ketones and the oxidation of secondary alcohols
-
-
?
additional information
?
-
the enzyme catalyzes the reduction of acetophenone derivatives to the corresponding (S)-chiral alcohols in an enantiomerically pure form. The substituents on the benzene ring of the aryl ketones exert some effect on the enzyme activity, although the influence is not dramatic. The enantioselectivity of the reduction is not affected by the substituents and pattern of the substitution. The alpha-chlorinated acetophenone shows a much higher activity than the unsubstituted one (more than 10 times)
-
-
?
additional information
?
-
-
substrate specificities and enantioselectivities of wild-type and mutant enzymes, overview. Molecular dynamics of wild-type ADH-A (PDB ID 3jv7) and the A2 variant (PDB ID 5o8q) in complex with alcohols (R)- and (S)-4-phenyl-2-butanol
-
-
-
additional information
?
-
-
substrate specificities and enantioselectivities of wild-type and mutant enzymes, overview. Molecular dynamics of wild-type ADH-A (PDB ID 3jv7) and the A2 variant (PDB ID 5o8q) in complex with alcohols (R)- and (S)-4-phenyl-2-butanol
-
-
-
additional information
?
-
the enzyme undergoes a substantial conformational change in the apo-holo transition, accompanied by loop movements at the domain interface
-
-
?
additional information
?
-
-
the enzyme undergoes a substantial conformational change in the apo-holo transition, accompanied by loop movements at the domain interface
-
-
?
additional information
?
-
-
constitutive enzyme
-
-
?
additional information
?
-
-
substrate specificity and stereospecificity, substrate binding pocket structure of the 3 isozymes, involving Met294, Trp57, and Trp93
-
-
?
additional information
?
-
-
effects of pressure on deuterium isotope effects of yeast alcohol dehydrogenase using alternative substrates
-
-
?
additional information
?
-
a quasi-vibrational process is detected with the use of HEPES buffer in the presence of alcohol: NADH is rapidly oxidized to NAD+ by ubiquinone and NAD+ is then slowly reduced to NADH by the alcohol. The NADH:ubiquinone-and alcohol:NAD+-reductase reactions are partially separated in time caused by considerable differences in the values of binding constants of NADH and NAD+ molecules
-
-
-
additional information
?
-
the enzyme acts as aldehyde reductase with catalytic functions for reduction of at least six aldehydes, including two furan aldehydes (furfural and 5-hydroxymethylfurfural), three aliphatic aldehydes (acetaldehyde, glycolaldehyde, and 3-methylbutanal), and an aromatic aldehyde (benzaldehyde) with NADH or NADPH as the cofactor. Particularly, Ymr152wp displays the highest specific activity (190.86 U/mg), and the best catalytic rate constant (Kcat), catalytic efficiency (Kcat/Km), and affinity (Km) when acetaldehyde is used as the substrate with NADH as the cofactor. Although Ymr152wp is grouped into the QOR family, no quinone reductase activity was observed using typical quinones as the substrates. No activity with formaldehyde, propionaldehyde, butyraldehyde, glutaraldehyde, 1,2-naphthoquinone, 9,10-phenanthrenequinone, p-benzoquinone, acetone, and acetylacetone. Substrate specificity, overview
-
-
-
additional information
?
-
a quasi-vibrational process is detected with the use of HEPES buffer in the presence of alcohol: NADH is rapidly oxidized to NAD+ by ubiquinone and NAD+ is then slowly reduced to NADH by the alcohol. The NADH:ubiquinone-and alcohol:NAD+-reductase reactions are partially separated in time caused by considerable differences in the values of binding constants of NADH and NAD+ molecules
-
-
-
additional information
?
-
-
the enzyme oxidizes alcohols to aldehydes or ketones both for detoxification and metabolic purposes
-
-
?
additional information
?
-
almost no activity with D-arabinonate, D-lyxonate, D-galactonate, glycerol, meso-erythritol, D-ribitol, D-arabitol, D-xylitol, and D-mannitol. No activity with propanal, butanal, hexanal, and 4-oxobutanoic acid
-
-
?
additional information
?
-
the physiological direction of the catalytic reaction is reduction rather than oxidation
-
-
?
additional information
?
-
the physiological direction of the catalytic reaction is reduction rather than oxidation
-
-
?
additional information
?
-
-
the physiological direction of the catalytic reaction is reduction rather than oxidation
-
-
?
additional information
?
-
the enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-ketoesters, but is poorly active on aliphatic, cyclic and aromatic alcohols, showing no activity on aldehydes
-
-
?
additional information
?
-
-
the enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-ketoesters, but is poorly active on aliphatic, cyclic and aromatic alcohols, showing no activity on aldehydes
-
-
?
additional information
?
-
the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl
-
-
?
additional information
?
-
the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl
-
-
?
additional information
?
-
-
the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl
-
-
?
additional information
?
-
enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes
-
-
?
additional information
?
-
enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes
-
-
?
additional information
?
-
-
enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes
-
-
?
additional information
?
-
the physiological direction of the catalytic reaction is reduction rather than oxidation
-
-
?
additional information
?
-
the physiological direction of the catalytic reaction is reduction rather than oxidation
-
-
?
additional information
?
-
the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl
-
-
?
additional information
?
-
the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl
-
-
?
additional information
?
-
the enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-ketoesters, but is poorly active on aliphatic, cyclic and aromatic alcohols, showing no activity on aldehydes
-
-
?
additional information
?
-
enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes
-
-
?
additional information
?
-
enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes
-
-
?
additional information
?
-
alcohol dehydrogenase catalyzes a reversible reaction of converting the aldehydes into alcohol
-
-
-
additional information
?
-
alcohol dehydrogenase catalyzes a reversible reaction of converting the aldehydes into alcohol
-
-
-
additional information
?
-
-
ethanol dehydrogenase activity of Thermoanaerobium brockii is both NAD and NADP linked, reversible, and not inhibited by low levels of reaction products
-
-
?
additional information
?
-
enzyme reduces aldehydes to (R)-alcohols with more than 99.8% enantiomeric excess
-
-
?
additional information
?
-
-
enzyme shows high substrate specificity towards primary aliphatic alcohols, no activity with 2-butanol, tert-butanol, isoamyl alcohol, isobutyl alcohol, 1,6-hexadiol, and mono-, di-, and triethanolamine
-
-
?
additional information
?
-
TADH is a NAD(H)-dependent enzyme and shows a very broad substrate spectrum producing exclusively the (S)-enantiomer in high enantiomeric excess (more than 99%) during asymmetric reduction of ketones
-
-
?
additional information
?
-
ADH exhibits a clear preference for primary alcohols and corresponding aldehydes for aliphatic substrates, in the oxidative direction activity steeply increases with chain length until 1-propanol and then decreases slightly again with growing chain length, alpha,beta-unsaturated ketones like 3-penten-2-one and cyclohexenone are not converted by ADH, almost no conversion of methanol (0.2%) and (+)-carvone (0.4%) is detected
-
-
?
additional information
?
-
TADH is a NAD(H)-dependent enzyme and shows a very broad substrate spectrum producing exclusively the (S)-enantiomer in high enantiomeric excess (more than 99%) during asymmetric reduction of ketones
-
-
?
additional information
?
-
ADH exhibits a clear preference for primary alcohols and corresponding aldehydes for aliphatic substrates, in the oxidative direction activity steeply increases with chain length until 1-propanol and then decreases slightly again with growing chain length, alpha,beta-unsaturated ketones like 3-penten-2-one and cyclohexenone are not converted by ADH, almost no conversion of methanol (0.2%) and (+)-carvone (0.4%) is detected
-
-
?
additional information
?
-
-
preference for reduction of aromatic ketones and alpha-keto esters, and poor activity on aromatic alcohols and aldehydes
-
-
?
additional information
?
-
-
the enzyme transfers the pro-S hydrogen of [4R-(2)H]NADH and exhibits Prelog specificity
-
-
?
additional information
?
-
-
the enzyme transfers the pro-S hydrogen of [4R-(2)H]NADH and exhibits Prelog specificity
-
-
?
additional information
?
-
-
preference for reduction of aromatic ketones and alpha-keto esters, and poor activity on aromatic alcohols and aldehydes
-
-
?
additional information
?
-
enzyme selectively reduces the C=O bond of allylic aldehydes/ketones to the corresponding alpha,beta-unsaturated alcohols and also has the capacity of stereoselectively reducing aromatic ketones to (S)-enantioselective alcohols. The enzyme preferentially catalyzes oxidation of allylic/benzyl aldehydes
-
-
?
additional information
?
-
-
key enzyme in ethanol production
-
-
?
additional information
?
-
-
substrate specificity and enantiospecificity, overview. The (R)-specific alcohol dehydrogenase requires NADH and reduces various kinds of carbonyl compounds, including ketones and aldehydes. AFPDH reduces acetylpyridine derivatives, beta-keto esters, and some ketones compounds with high enantiospecificity, overview. No activity with 2-chlorobenzaldehyde and 2-tetralone, poor activity with 1-tetralone, pyruvate, 2-oxobutyrate, oxalacetate, cyclopentanone, cyclohexanone, cycloheptanone, and dipropylketone. No activity with 1,2-propanediol, 3-chloro-1,2-propanediol, 3-bromo-1,2-propanediol, glycerol, 1-pentanol, poor activity with 1-butanol, 1-propanol, ethanol, and methanol
-
-
?
additional information
?
-
-
substrate specificity and enantiospecificity, overview. The (R)-specific alcohol dehydrogenase requires NADH and reduces various kinds of carbonyl compounds, including ketones and aldehydes. AFPDH reduces acetylpyridine derivatives, beta-keto esters, and some ketones compounds with high enantiospecificity, overview. No activity with 2-chlorobenzaldehyde and 2-tetralone, poor activity with 1-tetralone, pyruvate, 2-oxobutyrate, oxalacetate, cyclopentanone, cyclohexanone, cycloheptanone, and dipropylketone. No activity with 1,2-propanediol, 3-chloro-1,2-propanediol, 3-bromo-1,2-propanediol, glycerol, 1-pentanol, poor activity with 1-butanol, 1-propanol, ethanol, and methanol
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.25
(+/-)-3-methylcyclohexanone
-
-
9.1
(2E)-but-2-en-1-ol
pH 8.0, 55°C
3.3
(2E)-but-2-enal
pH 6.5, 65°C
0.41 - 37.4
(R)-2-butanol
0.22 - 31.7
(R)-2-pentanol
0.02
(R)-2-phenylpropanol
-
-
1.28 - 1.3
(R)-alpha-tetralol
4.2
(S)-(+)-1-indanol
-
65°C
4.2
(S)-(+)-alpha-tetraol
-
65°C
18.1
(S)-(-)-1-phenylethanol
-
65°C
18.1
(S)-1-phenylethanol
-
pH 10.5, 65°C
0.012 - 120
(S)-2-butanol
0.07 - 8.8
(S)-2-pentanol
0.157
(S)-2-phenylpropanol
-
-
1.05 - 4.2
(S)-alpha-tetralol
8.47
(S)-N-benzyl-3-pyrrolidinol
-
pH 8.0
1.65 - 15.84
1,2-propanediol
23.64 - 26.7
1,3-Propanediol
0.36
1,4-Butanediol
pH 10, 60°C
0.000036 - 0.0038
1-formyl-6-methylpyrene
0.000035 - 0.00149
1-formyl-8-methylpyrene
0.0005 - 0.012
1-formylpyrene
0.00028 - 0.0064
1-hydroxymethyl-6-methylpyrene
0.00031 - 0.059
1-hydroxymethyl-8-methylpyrene
0.00059 - 0.076
1-hydroxymethylpyrene
5.9
1-phenyl-1,2-propandione
-
pH 10.5, 65°C
5 - 12.1
1-phenyl-1,2-propanedione
0.025 - 244
1-phenylethanol
0.2
11-cis-retinal
-
reduction with NADH
0.018 - 0.28
11-cis-retinol
0.013 - 2.3
12-hydroxydodecanoate
0.056
12-Hydroxydodecanoic acid
-
-
0.086 - 0.1
12-hydroxylauric acid methyl ester
0.066
12-oxolauric acid methyl ester
wild-type, pH 8.0, 60°C
0.11 - 0.137
13-cis-retinal
0.257
13-cis-retinol
-
oxidation with NAD+
0.06
16-hydroxyhexadecanoate
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
11.9
2',3',4',5',6'-pentafluoroacetophenone
65°C, pH 5.0
6.3 - 23.5
2,2,2-trifluoroacetophenone
0.11
2,2-dichloroacetophenone
65°C, pH 5.0
0.44 - 1100
2,3-Butanediol
0.147
2-decanone
pH 8.0, 60°C
13
2-dehydro-3-deoxy-D-gluconate
at pH 7.9 and 25°C
310
2-deoxy-D-ribose
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.25
2-ethoxyethanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.0021 - 0.009
2-formylpyrene
1.16
2-Heptanone
pH 8.0, 60°C
5.01
2-Hexanone
pH 8.0, 60°C
0.00048 - 0.106
2-hydroxymethylpyrene
0.021
2-Methylcyclohexanol
-
-
2.7
2-methylpropan-1-ol
-
-
0.215
2-nonanone
pH 8.0, 60°C
0.286
2-Octanone
pH 8.0, 60°C
0.752
2-Pentanol
pH 8.0, 60°C
0.864
2-phenylpropionaldehyde
-
-
0.025 - 0.026
3,4-dihydro-retinal
0.024 - 0.028
3,4-dihydro-retinol
0.14 - 0.9
3-bromobenzyl alcohol
0.14
3-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.9 - 13
3-hydroxy-2-butanone
4.4
3-Methoxybenzaldehyde
-
65°C
1.2 - 3.3
3-Methoxybenzyl alcohol
1.2
3-methoxybenzylalcohol
7.5
3-methylbutan-2-one
-
-
9.9
3-Methylcyclohexanol
65°C, pH 8.0
66
3-methylcyclohexanone
65°C, pH 5.5
0.031
3-oxo-5beta-androstan-17beta-ol
-
-
0.032
3-Phenyl-1-propanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.033
3-phenylpropanol
-
-
0.128 - 0.31
3beta,12alpha-dihydroxy-5beta-cholanoate
0.149 - 0.182
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
0.35
3beta,7alpha-12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme 4
0.032 - 0.071
3beta-7alpha-dihydroxy-5beta-cholanoate
3.8 - 34.9
4-amino-1-butanol
0.12 - 0.4
4-bromobenzyl alcohol
0.12
4-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.28
4-carboxybenzaldehyde
0.14 - 1.11
4-chloroacetophenone
0.00048 - 0.0038
4-formylpyrene
0.004 - 0.015
4-hydroxy-retinol
0.00037 - 0.04
4-hydroxymethylpyrene
0.024 - 4.4
4-methoxybenzaldehyde
0.07 - 61
4-methoxybenzyl alcohol
1.3
4-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.131
4-methoxyphenylacetone
pH 8.0, 60°C
6.7 - 53.8
4-Methyl-1-pentanol
0.81
4-methylpentan-1-ol
-
-
0.017 - 0.027
4-oxo-retinal
26.9
5-amino-1-pentanol
pH 9.0, 25°C, recombinant enzyme
9.45
5-hydroxymethylfurfural
-
mutant S110P/Y295C, pH 6.7, 30°C
0.0036 - 0.04
5alpha-androstan-17beta-ol-3-one
0.011 - 0.046
5beta-androstan-17beta-ol-3-one
0.123 - 0.164
5beta-androstan-3beta-ol-17 one
0.003 - 0.058
5beta-androstan-3beta-ol-17-one
0.008 - 0.25
5beta-cholanic acid-3-one
0.047
5beta-Pregnan-21-ol-3,20-dione hemisuccinate
-
-
0.0036
5beta-pregnan-3,20-dione
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.027
5beta-Pregnan-3beta-ol-20-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
6.71
6-amino-1-hexanol
pH 9.0, 25°C, recombinant enzyme
0.16 - 0.242
9-cis-retinal
0.011 - 0.3
9-cis-retinol
0.0086 - 121
acetaldehyde
0.011 - 0.34
all-trans-retinal
0.009 - 0.059
all-trans-retinol
0.39
allyl alcohol
-
pH 7.3, 37°C
5.3
alpha-tetralol
-
65°C
0.0025 - 0.09
Anisaldehyde
0.007 - 49
benzyl alcohol
0.0145 - 0.8
benzylalcohol
86.8
Butan-2,3-diol
pH 8.8, 70°C
0.06 - 0.27
Butyraldehyde
0.14 - 0.25
capronaldehyde
0.03
cinnamaldehyde
-
pH 7.7, 60°C
0.11 - 4.58
cinnamyl alcohol
1
cis-4-methylcyclohexanol
-
-
4.2
cis-decahydro-1-naphthol
65°C, pH 8.0
0.71
crotyl alcohol
-
pH 7.3, 37°C
0.008 - 1900
Cyclohexanol
1.39 - 14.1
cyclohexanone
16
D-allonate
at pH 7.9 and 25°C
24
D-altronate
at pH 7.9 and 25°C
220
D-arabitol
at pH 7.9 and 25°C
12
D-gluconate
at pH 7.9 and 25°C
14
D-mannonate
at pH 7.9 and 25°C
16
D-ribonate
at pH 7.9 and 25°C
350
D-sorbitol
at pH 7.9 and 25°C
6
D-talonate
at pH 7.9 and 25°C
24
D-xylonate
at pH 7.9 and 25°C
16.6
ethyl 3-methyl-2-oxobutyrate
65°C, pH 5.0
1 - 5.3
ethyl benzoylformate
1
ethyl oxo(phenyl)acetate
-
pH 10.5, 65°C
5.3
ethyl pyruvate
-
pH 6.5, 50°C, recombinant enzyme
0.67
Glutaraldehyde
pH 6, 60°C
16.11 - 88.32
glycolaldehyde
0.81
isoborneol
65°C, pH 10.5
0.26 - 0.76
Isobutyraldehyde
197
Isopentanol
at pH 7.0 and 25°C
1.4
m-nitrobenzaldehyde
-
isoenzyme ADH-1, pH 7.5
6.5
methyl benzoylformate
65°C, pH 5.5
2.8
methyl o-chlorobenzoylformate
2.7
methyl oxo(phenyl)acetate
-
pH 10.5, 65°C
2.7
methylbenzoylformate
-
65°C
0.13 - 0.58
N-benzyl-3-pyrrolidinone
10.3
n-Pentanal
-
33°C, pH 8
0.011
octan-1-ol
-
enzyme form ADH-3
2.9 - 5.9
p-Methoxybenzaldehyde
6.9 - 22
p-methoxybenzyl alcohol
13.6
phenyl trifluoromethyl ketone
-
25°C, pH 7.0
0.19 - 0.91
phenylacetaldehyde
24.45 - 37.55
phenylglyoxylic acid
2.01
propanone
-
33°C, pH 7
3.4
R-(+)-1-phenylethanol
-
-
1.2
R-(+)-cis-verbenol
-
-
1.5
R-(+)-trans-bicyclo(2.2.1)-heptanol
-
-
3.9
S-(-)-1-phenylethanol
-
-
0.95
S-(-)-2-methylbutan-1-ol
-
-
3.8
S-(-)-cis-bicyclo(2.2.1)-heptanol
-
-
0.85
S-(-)-trans-bicyclo(2.2.1)-heptanol
-
-
568.84
tert-butanol
pH 7.5, 23°C
0.694
tert-butyl acetoacetate
pH 8.0, 60°C
8.5
tetralin-1-ol
65°C, pH 10.5
1.1
trans-4-methylcyclohexanol
-
-
0.009 - 0.23
trans-cinnamaldehyde
0.2
tryptophol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.034 - 11
Vanillyl alcohol
additional information
additional information
-
2.4
(R)-1-indanol
pH 8.0, 65°C
2.4
(R)-1-indanol
65°C, pH 8.0
0.41
(R)-2-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
37.4
(R)-2-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.22
(R)-2-pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
11.7
(R)-2-pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.28
(R)-alpha-tetralol
pH 8.0, 65°C
1.3
(R)-alpha-tetralol
65°C, pH 8.0
4.2
(S)-1-indanol
-
pH 10.5, 65°C
6.4
(S)-1-indanol
pH 8.0, 65°C
6.4
(S)-1-indanol
65°C, pH 8.0
0.012
(S)-2-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
29.8
(S)-2-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.07
(S)-2-pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
8.8
(S)-2-pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.05
(S)-alpha-tetralol
pH 8.0, 65°C
1.05
(S)-alpha-tetralol
65°C, pH 8.0
4.2
(S)-alpha-tetralol
-
pH 10.5, 65°C
1.65
1,2-propanediol
-
-
11.5
1,2-propanediol
-
pH 8.0, 60°C, recombinant enzyme
15.84
1,2-propanediol
-
-
23.64
1,3-Propanediol
pH 7.5, 23°C
26.7
1,3-Propanediol
-
pH 9.0, 60°C, recombinant enzyme
0.08
1-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.17
1-butanol
-
isoenzyme ADH-3, pH 10.0
0.596
1-butanol
pH 8.0, 60°C
1.6
1-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.45
1-butanol
pH 7.5, 23°C
17
1-butanol
-
isoenzyme ADH-1, pH 10.0
230
1-butanol
-
isoenzyme ADH-1, pH 7.5
0.000036
1-formyl-6-methylpyrene
isozyme ADH4, at 21-23°C
0.00032
1-formyl-6-methylpyrene
isozyme ADH2, at 21-23°C
0.0009
1-formyl-6-methylpyrene
isozyme ADH1C, at 21-23°C
0.0038
1-formyl-6-methylpyrene
isozyme ADH3, at 21-23°C
0.000035
1-formyl-8-methylpyrene
isozyme ADH4, at 21-23°C
0.00024
1-formyl-8-methylpyrene
isozyme ADH2, at 21-23°C
0.00131
1-formyl-8-methylpyrene
isozyme ADH1C, at 21-23°C
0.00149
1-formyl-8-methylpyrene
isozyme ADH3, at 21-23°C
0.0005
1-formylpyrene
isozyme ADH1C, at 21-23°C
0.00055
1-formylpyrene
isozyme ADH3, at 21-23°C
0.00092
1-formylpyrene
isozyme ADH4, at 21-23°C
0.012
1-formylpyrene
isozyme ADH2, at 21-23°C
0.038
1-Heptanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.21
1-Heptanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.67
1-Heptanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.035
1-Hexanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.04
1-Hexanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.147
1-Hexanol
pH 8.0, 60°C
0.88
1-Hexanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.00028
1-hydroxymethyl-6-methylpyrene
isozyme ADH4, at 21-23°C
0.00057
1-hydroxymethyl-6-methylpyrene
isozyme ADH3, at 21-23°C
0.00115
1-hydroxymethyl-6-methylpyrene
isozyme ADH1C, at 21-23°C
0.0064
1-hydroxymethyl-6-methylpyrene
isozyme ADH2, at 21-23°C
0.00031
1-hydroxymethyl-8-methylpyrene
isozyme ADH3, at 21-23°C
0.0016
1-hydroxymethyl-8-methylpyrene
isozyme ADH4, at 21-23°C
0.0064
1-hydroxymethyl-8-methylpyrene
isozyme ADH2, at 21-23°C
0.059
1-hydroxymethyl-8-methylpyrene
isozyme ADH1C, at 21-23°C
0.00059
1-hydroxymethylpyrene
isozyme ADH3, at 21-23°C
0.00075
1-hydroxymethylpyrene
isozyme ADH1C, at 21-23°C
0.0283
1-hydroxymethylpyrene
isozyme ADH4, at 21-23°C
0.076
1-hydroxymethylpyrene
isozyme ADH2, at 21-23°C
5.1
1-Indanol
-
65°C
8.7
1-Indanol
65°C, pH 10.5
27.6
1-Indanone
-
65°C
27.6
1-Indanone
-
pH 10.5, 65°C
0.025
1-Octanol
-
isoenzyme ADH-3, pH 10.0
0.1
1-Octanol
-
isoenzyme ADH-2
0.39
1-Octanol
isozyme ADH3, at 21-23°C
0.5
1-Octanol
-
isoenzyme ADH-1, pH 7.5
0.51
1-Octanol
-
isoenzyme ADH-2, pH 10.0
0.027
1-Pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.08
1-Pentanol
-
isoenzyme ADH-3, pH 10.0
0.26
1-Pentanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.396
1-Pentanol
pH 8.0, 60°C
1.9
1-Pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.1
1-Pentanol
-
isoenzyme ADH-1, pH 10.0
78
1-Pentanol
-
isoenzyme ADH-2, pH 10.0
5
1-phenyl-1,2-propanedione
65°C, pH 5.0
5.9
1-phenyl-1,2-propanedione
-
65°C
12.1
1-phenyl-1,2-propanedione
65°C, pH 5.5
0.025
1-phenylethanol
-
-
244
1-phenylethanol
pH 9.0, 70°C
0.24
1-propanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.33
1-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.33
1-propanol
-
native enzyme, at 55°C
0.6
1-propanol
-
wild type enzyme, at 65°C
1.03
1-propanol
pH 8.0, 60°C
1.7
1-propanol
-
mutant enzyme N249Y, at 65°C
2.4
1-propanol
-
carboxymethylated enzyme, at 55°C
6.5
1-propanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
39.8
1-propanol
pH 7.5, 23°C
0.018
11-cis-retinol
-
isozyme ADH1B2, pH 7.5, 25°C
0.035
11-cis-retinol
-
isozyme ADH1B1, pH 7.5, 25°C
0.28
11-cis-retinol
-
oxidation with NAD+
0.013
12-hydroxydodecanoate
-
isoenzyme ADH-3, pH 10.0
0.02
12-hydroxydodecanoate
-
isoenzyme BB-ADH
0.04
12-hydroxydodecanoate
-
enzyme form ADH-3
0.047
12-hydroxydodecanoate
-
-
0.048
12-hydroxydodecanoate
-
-
0.05
12-hydroxydodecanoate
-
isoenzyme BB-ADH and isoenzyme ADH-TT
0.06
12-hydroxydodecanoate
-
isoenzyme AA-ADH
0.1
12-hydroxydodecanoate
-
-
0.1
12-hydroxydodecanoate
-
isoenzyme ADH-1, pH 10.0
0.23
12-hydroxydodecanoate
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
1.4
12-hydroxydodecanoate
-
isoenzyme ADH-1, pH 10.0
2.3
12-hydroxydodecanoate
-
isoenzyme TT-ADH
0.086
12-hydroxylauric acid methyl ester
wild-type, pH 8.0, 60°C
0.1
12-hydroxylauric acid methyl ester
mutant C257L, pH 8.0, 60°C
0.11
13-cis-retinal
-
oxidation with NAD+
0.137
13-cis-retinal
-
reduction with NADH
6.3
2,2,2-trifluoroacetophenone
65°C, pH 5.0
11.2
2,2,2-trifluoroacetophenone
-
65°C
11.2
2,2,2-trifluoroacetophenone
-
pH 10.5, 65°C
23.5
2,2,2-trifluoroacetophenone
65°C, pH 5.5
0.44
2,3-Butanediol
-
pH 8.8, 45°C, wild-type enzyme, cofactor: NAP+
0.91
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211S, cofactor: NADP+
1.3
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NADP+, wild-type enzyme
1.5
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NADP+
5.5
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NADP+
9.6
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211C, cofactor: NADP+
12
2,3-Butanediol
-
pH 8.8, 45°C, wild-type enzyme, cofactor: NAD+
17
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NADP+
29
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NAD+, wild-type enzyme
61.3
2,3-Butanediol
pH 9.0, 70°C
76
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NAD+
90
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NADP+
100
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211S, cofactor: NAD+
110
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NAD+
200
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NADP+, mutant enzyme K249G/H255R
240
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211C, cofactor: NAD+
260
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NAD+
690
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NAD+, mutant enzyme K249G/H255R
1100
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NAD+
1.05
2-butanol
pH 8.0, 60°C
11.9
2-butanol
-
33°C, pH 8
23.82
2-butanol
pH 7.5, 23°C
0.35
2-Buten-1-ol
-
isoenzyme ADH-3, pH 10.0
2.5
2-Buten-1-ol
-
isoenzyme ADH-1, pH 10.0
60
2-Buten-1-ol
-
isoenzyme ADH-2, pH 10.0
0.0021
2-formylpyrene
isozyme ADH1C, at 21-23°C
0.0029
2-formylpyrene
isozyme ADH2, at 21-23°C
0.0069
2-formylpyrene
isozyme ADH4, at 21-23°C
0.009
2-formylpyrene
isozyme ADH3, at 21-23°C
0.00048
2-hydroxymethylpyrene
isozyme ADH1C, at 21-23°C
0.0044
2-hydroxymethylpyrene
isozyme ADH2, at 21-23°C
0.033
2-hydroxymethylpyrene
isozyme ADH4, at 21-23°C
0.106
2-hydroxymethylpyrene
isozyme ADH3, at 21-23°C
5.15
2-Pentanone
pH 8.0, 60°C
0.6
2-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.6
2-propanol
-
native enzyme, at 55°C
2.44
2-propanol
pH 8.0, 60°C
19.6
2-propanol
-
33°C, pH 8
53
2-propanol
-
carboxymethylated enzyme, at 55°C
57.5
2-propanol
-
25°C, pH 7.0
57.8
2-propanol
-
pH 7.7, 60°C
268
2-propanol
-
wilde-type enzyme ADh1
560
2-propanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.025
3,4-dihydro-retinal
-
pH 7.5, 25°C, isozyme ADH1B2
0.026
3,4-dihydro-retinal
-
pH 7.5, 25°C, isozyme ADH4
0.024
3,4-dihydro-retinol
-
pH 7.5, 25°C, isozyme ADH1B2
0.028
3,4-dihydro-retinol
-
pH 7.5, 25°C, isozyme ADH4
0.14
3-bromobenzyl alcohol
-
native enzyme, at 55°C
0.9
3-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.9
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADH, wild-type enzyme
5
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADPH, mutant enzyme K249G/H255R
6.7
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADPH, wild-type enzyme
13
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADH, mutant enzyme K249G/H255R
1.2
3-Methoxybenzyl alcohol
-
native enzyme, at 55°C
3.3
3-Methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
1.2
3-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.2
3-methoxybenzylalcohol
-
in the presence of 5 mM benzylalcohol, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.27
3-Pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.128
3beta,12alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 2
0.16
3beta,12alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 3
0.248
3beta,12alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 4
0.31
3beta,12alpha-dihydroxy-5beta-cholanoate
-
isoenzyme I
0.149
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme I
0.16
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme 2
0.182
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme 3
0.032
3beta-7alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 3
0.064
3beta-7alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 2
0.066
3beta-7alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 1
0.071
3beta-7alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 4
3.8
4-amino-1-butanol
pH 11.0, 25°C, recombinant enzyme
17.8
4-amino-1-butanol
pH 9.0, 25°C, recombinant enzyme
34.9
4-amino-1-butanol
pH 7.0, 25°C, recombinant enzyme
0.12
4-bromobenzyl alcohol
-
native enzyme, at 55°C
0.4
4-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.28
4-carboxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.28
4-carboxybenzaldehyde
-
native enzyme, at 55°C
0.14
4-chloroacetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.53
4-chloroacetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
1.11
4-chloroacetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.00048
4-formylpyrene
isozyme ADH4, at 21-23°C
0.001
4-formylpyrene
isozyme ADH1C, at 21-23°C
0.0021
4-formylpyrene
isozyme ADH3, at 21-23°C
0.0038
4-formylpyrene
isozyme ADH2, at 21-23°C
0.004
4-hydroxy-retinol
-
pH 7.5, 25°C, isozyme ADH1B1
0.011
4-hydroxy-retinol
-
pH 7.5, 25°C, isozyme ADH1B2
0.015
4-hydroxy-retinol
-
pH 7.5, 25°C, isozyme ADH4
0.00037
4-hydroxymethylpyrene
isozyme ADH3, at 21-23°C
0.001
4-hydroxymethylpyrene
isozyme ADH1C, at 21-23°C
0.0029
4-hydroxymethylpyrene
isozyme ADH4, at 21-23°C
0.04
4-hydroxymethylpyrene
isozyme ADH2, at 21-23°C
0.024
4-methoxybenzaldehyde
-
wild type enzyme, at 65°C
0.14
4-methoxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.14
4-methoxybenzaldehyde
-
native enzyme, at 55°C
0.86
4-methoxybenzaldehyde
-
mutant enzyme N249Y, at 65°C
4.4
4-methoxybenzaldehyde
-
pH 6.0, 65°C
0.07
4-methoxybenzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.12
4-methoxybenzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.23
4-methoxybenzyl alcohol
-
wild type enzyme, at 65°C
1.13
4-methoxybenzyl alcohol
pH 8.0, 60°C
1.24
4-methoxybenzyl alcohol
-
mutant enzyme N249Y, at 65°C
1.3
4-methoxybenzyl alcohol
-
native enzyme, at 55°C
1.3
4-methoxybenzyl alcohol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.4
4-methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
61
4-methoxybenzyl alcohol
-
65°C
61
4-methoxybenzyl alcohol
-
pH 10.5, 65°C
6.7
4-Methyl-1-pentanol
-
mutant enzyme W54L
53.8
4-Methyl-1-pentanol
-
wilde-type enzyme ADh1
0.017
4-oxo-retinal
-
pH 7.5, 25°C, isozyme ADH4
0.025
4-oxo-retinal
-
pH 7.5, 25°C, isozyme ADH1B1
0.027
4-oxo-retinal
-
pH 7.5, 25°C, isozyme ADH1B2
0.0036
5alpha-androstan-17beta-ol-3-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.021
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 2
0.025
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 2
0.027
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 4
0.04
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 1
0.011
5beta-androstan-17beta-ol-3-one
-
pH 7.3, 37°C, recombinant enzyme
0.046
5beta-androstan-17beta-ol-3-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.123
5beta-androstan-3beta-ol-17 one
-
isoenzyme 4
0.125
5beta-androstan-3beta-ol-17 one
-
isoenzyme 2
0.14
5beta-androstan-3beta-ol-17 one
-
isoenzyme 3
0.164
5beta-androstan-3beta-ol-17 one
-
isoenzyme 1
0.003
5beta-androstan-3beta-ol-17-one
-
pH 7.3, 37°C, recombinant enzyme
0.058
5beta-androstan-3beta-ol-17-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.008
5beta-cholanic acid-3-one
-
pH 7.3, 37°C, recombinant enzyme
0.25
5beta-cholanic acid-3-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.16
9-cis-retinal
-
oxidation with NAD+
0.242
9-cis-retinal
-
reduction with NADH
0.011
9-cis-retinol
-
isozyme ADH1B1, pH 7.5, 25°C
0.023
9-cis-retinol
-
isozyme ADH1B2, pH 7.5, 25°C
0.3
9-cis-retinol
-
oxidation with NAD+
0.0086
acetaldehyde
Sporotrichum pulverulentum
-
-
0.013
acetaldehyde
-
70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.059
acetaldehyde
-
40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.085
acetaldehyde
-
isoenzyme beta1,beta1
0.085
acetaldehyde
-
isoenzyme beta1,beta1, 0.1 M sodium phosphate buffer, pH 7.5, at 25 °C
0.086
acetaldehyde
-
enzyme form ADH I
0.1
acetaldehyde
-
pyrazole-sensitive enzyme, pH 7.0
0.217
acetaldehyde
-
isoenzyme 3
0.22
acetaldehyde
-
wild-type, pH 6.7, 30°C
0.23
acetaldehyde
-
isoenzyme 2
0.24
acetaldehyde
-
isoenzyme gamma2,gamma2
0.24
acetaldehyde
-
isoenzymes beta2,beta2
0.24
acetaldehyde
-
isoenzyme beta2,beta2, 0.1 M sodium phosphate buffer, pH 7.5, 25°C
0.276
acetaldehyde
-
isoenzyme 4
0.3
acetaldehyde
-
isozyme ADH I, pH 5.5, 25°C
0.31
acetaldehyde
-
isoenzyme 1
0.33
acetaldehyde
-
isoenzyme gamma1,gamma1
0.34
acetaldehyde
isozyme ADH1C, at 21-23°C
0.364
acetaldehyde
wild-type, pH 8.0, 60°C
0.67
acetaldehyde
pH 6.0, 22°C, recombinant enzyme
0.74
acetaldehyde
pH 7.0, 30°C, with NADH
0.83
acetaldehyde
-
mutant S110P/Y295C, pH 6.7, 30°C
0.92
acetaldehyde
-
33°C, pH 7
1.2
acetaldehyde
pH 7.0, temperature not specified in the publication
1.25
acetaldehyde
-
isoenzyme II
1.3
acetaldehyde
-
enzyme form ADH II
1.45
acetaldehyde
-
isoenzyme I
1.8
acetaldehyde
Megalodesulfovibrio gigas
-
-
1.9
acetaldehyde
-
pH 8.5
2.6
acetaldehyde
-
pH 8.0, 60°C, recombinant enzyme
3.4
acetaldehyde
-
isoenzyme beta3,beta3, 0.1 M sodium phospahte buffer, pH 7.5, 25°C
4
acetaldehyde
pH 7.0, temperature not specified in the publication
4.3
acetaldehyde
-
isoenzyme alpha,alpha
4.71
acetaldehyde
pH 8, 60°C
7.43
acetaldehyde
-
pH 7.0, 30°C
11.5
acetaldehyde
-
pyrazole-insensitive enzyme, pH 7.0
12.7
acetaldehyde
isozyme ADH4, at 21-23°C
24
acetaldehyde
-
isozyme ADH II, pH 6.0, 25°C
26
acetaldehyde
isozyme ADH2, at 21-23°C
28
acetaldehyde
pH 6.0, temperature not specified in the publication
34
acetaldehyde
-
wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+
70
acetaldehyde
-
mutant enzyme ADH1-1S1108
85
acetaldehyde
-
wild-type enzyme ADH1-1S
121
acetaldehyde
-
alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+
6.5
acetoin
reduction reaction, pH 6.1, 70°C
30.2
acetoin
pH 6.0, 70°C
6.6
acetone
-
-
0.2
acetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.4
acetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.66
acetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.3
acycloNAD+
-
substrate butan-1-ol, pH 8.0, 25°C
0.33
acycloNAD+
-
substrate hexan-1-ol, pH 8.0, 25°C
0.33
acycloNAD+
-
substrate propan-1-ol, pH 8.0, 25°C
0.36
acycloNAD+
-
substrate ethanol, pH 8.0, 25°C
0.61
acycloNAD+
-
substrate benzyl alcohol, pH 8.0, 25°C
0.011
all-trans-retinal
-
pH 7.5, 25°C, isozyme ADH1B1
0.012
all-trans-retinal
-
pH 7.5, 25°C, isozyme ADH1B2
0.034
all-trans-retinal
-
pH 7.5, 25°C, isozyme ADH4
0.26
all-trans-retinal
-
oxidation with NAD+
0.34
all-trans-retinal
-
reduction with NADH
0.009
all-trans-retinol
-
-
0.02
all-trans-retinol
-
isoenzyme TT-ADH
0.023
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH4
0.03
all-trans-retinol
-
isoenzyme BB-ADH
0.03
all-trans-retinol
-
isozyme ADH1B1, pH 7.5, 25°C
0.03
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH1B1
0.033
all-trans-retinol
-
isozyme ADH1B2, pH 7.5, 25°C
0.033
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH1B2
0.05
all-trans-retinol
-
isoenzyme AA-ADH
0.059
all-trans-retinol
-
oxidation with NAD+
5.8
alpha-tetralone
-
65°C
5.8
alpha-tetralone
-
pH 10.5, 65°C
0.0025
Anisaldehyde
-
-
0.03
benzaldehyde
-
wild type enzyme, at 65°C
0.03
benzaldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.07
benzaldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.2
benzaldehyde
-
carboxymethylated enzyme, at 55°C
0.26
benzaldehyde
-
mutant enzyme N249Y, at 65°C
0.28
benzaldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.3
benzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.3
benzaldehyde
-
native enzyme, at 55°C
0.333
benzaldehyde
pH 8.0, 60°C
0.4
benzaldehyde
pH 6.5, 65°C
2 - 3
benzaldehyde
in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
14
benzaldehyde
-
isozyme ADH2, in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
33
benzaldehyde
-
isozyme ADH1, in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
0.007
benzyl alcohol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.013
benzyl alcohol
-
enzyme form ADH-2
0.05
benzyl alcohol
-
isoenzyme ADH-3, pH 10.0
0.12
benzyl alcohol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.26
benzyl alcohol
-
wild type enzyme, at 65°C
0.26
benzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.8
benzyl alcohol
-
native enzyme, at 55°C
0.8
benzyl alcohol
mutant Y25A/W49F/W167Y, 30°C, pH not specified in the publication
1.4
benzyl alcohol
-
isoenzyme ADH-1, pH 10.0
1.5
benzyl alcohol
-
mutant enzyme N249Y, at 65°C
1.5
benzyl alcohol
mutant W87A, pH 7.0, 30°C
2
benzyl alcohol
-
carboxymethylated enzyme, at 55°C
3.1
benzyl alcohol
mutant W49F/W167Y, 30°C, pH not specified in the publication
3.8
benzyl alcohol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.2
benzyl alcohol
mutant V260A, 30°C, pH not specified in the publication
5.4
benzyl alcohol
mutant Y25A/W49F/W167Y/V260A, 30°C, pH not specified in the publication
5.43
benzyl alcohol
pH 8.0, 60°C
6
benzyl alcohol
mutant W49F/W87F, 30°C, pH not specified in the publication
6.8
benzyl alcohol
wild-type, 30°C, pH not specified in the publication
6.9
benzyl alcohol
wild-type, pH 7.0, 30°C
7.2
benzyl alcohol
mutant Y25A, pH 7.0, 30°C
8.2
benzyl alcohol
mutant W49F/W167Y/V260A, 30°C, pH not specified in the publication
10.1
benzyl alcohol
mutant Y25A/W49F/W87F/V260A, 30°C, pH not specified in the publication
10.9
benzyl alcohol
mutant Y25A/W49F/W87F, 30°C, pH not specified in the publication
12.9
benzyl alcohol
mutant W49F/W87F/V260A, 30°C, pH not specified in the publication
14
benzyl alcohol
pH 8.0, 55°C
16
benzyl alcohol
mutant A25Y, pH 7.0, 30°C
16.5
benzyl alcohol
mutant Y25A, 30°C, pH not specified in the publication
24
benzyl alcohol
in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
29.4
benzyl alcohol
wild-type, pH 7.0, 30°C
34
benzyl alcohol
-
isozyme ADH1, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
49
benzyl alcohol
-
isozyme ADH2, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
0.0145
benzylalcohol
in 50 mM glycine-NaOH at pH 10.0
0.44
benzylalcohol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
0.8
benzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.24
beta-NAD+
-
in presence of 0.5 mM SDS
0.243
beta-NAD+
-
in presence of 0.5 mM SDS
0.35
beta-NAD+
-
in presence of 1 mM SDS
0.71
beta-NAD+
-
in presence of 3 mM SDS
1.23
beta-NAD+
-
in presence of 50 mM SDS
2.29
beta-NAD+
-
in presence of 15 mM SDS
0.055
butan-1-ol
-
-
2
butan-1-ol
-
enzyme form ADH-3
5.65
butan-1-ol
cosubstrate NAD+, pH 7.0, 30°C
0.35
butan-2-ol
-
-
93.3
butan-2-ol
-
wilde-type enzyme ADh1
0.48
Butanal
cosubstrate NADH, pH 7.0, 30°C
3
Butanal
-
isoenzyme ADH-1, pH 7.5
4.24
Butanal
-
33°C, pH 7
71.98
Butanal
-
pH 7.0, 30°C
0.012
butanol
-
pyrazole-sensitive enzyme, pH 7.5
0.02
butanol
-
pyrazole-sinensitive enzyme, pH 10.0
0.06
butanol
-
pyrazole-insensitive enzyme, pH 7.5
0.09
butanol
-
pyrazole-sensitive enzyme, pH 10.0
29.4
butanol
-
mutant enzyme W54L
53.9
butanol
-
wilde-type enzyme ADh1
0.06
Butyraldehyde
-
-
0.07
Butyraldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.108
Butyraldehyde
wild-type, pH 8.0, 60°C
0.13
Butyraldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.27
Butyraldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.14
capronaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.22
capronaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.25
capronaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.11
cinnamyl alcohol
-
pH 7.7, 60°C
1.5
cinnamyl alcohol
-
mutant enzyme W54L
4.58
cinnamyl alcohol
-
wilde-type enzyme ADh1
5.1
cycloheptanol
65°C, pH 10.5
9.3
cycloheptanol
pH 8.0, 65°C
9.3
cycloheptanol
65°C, pH 8.0
0.008
Cyclohexanol
-
isoenzyme alpha,gamma1
0.03
Cyclohexanol
-
wild type enzyme, at 65°C
0.03
Cyclohexanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.04
Cyclohexanol
-
isoenzyme BB-ADH
0.045
Cyclohexanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.045
Cyclohexanol
-
native enzyme, at 55°C
0.23
Cyclohexanol
-
carboxymethylated enzyme, at 55°C
0.703
Cyclohexanol
pH 8.0, 60°C
0.76
Cyclohexanol
-
isoenzyme AA-ADH
1.9
Cyclohexanol
-
mutant enzyme N249Y, at 65°C
2.09
Cyclohexanol
in 200 mM bicine, pH 9.0, at 60°C
2.2
Cyclohexanol
-
isoenzyme ADH-3, pH 10.0
4.5
Cyclohexanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
5.2
Cyclohexanol
-
enzyme form ADH-3
23
Cyclohexanol
-
isoenzyme beta1,beta1
210
Cyclohexanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
220
Cyclohexanol
-
isoenzyme ADH-1, pH 10.0
320
Cyclohexanol
-
isoenzyme TT-ADH
1900
Cyclohexanol
-
isoenzyme ADH-2, pH 10.0
1.39
cyclohexanone
pH 8.0, 60°C
3.68
cyclohexanone
in 200 mM bicine, pH 6.0, at 60°C
0.0172
ethanol
in 50 mM glycine-NaOH at pH 10.0
0.022
ethanol
-
70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.022
ethanol
-
isoenzyme beta1,beta1, 0.1 M sodium phosphate buffer, pH 7.5, at 25 °C
0.049
ethanol
-
isoenzyme beta1,beta1
0.147
ethanol
20°C, pH 9.0
0.15
ethanol
Megalodesulfovibrio gigas
-
-
0.166
ethanol
recombinant His-tagged wild-type enzyme, pH 7.5, temperature not specified in the publication
0.168
ethanol
30°C, pH 9.0
0.18
ethanol
-
pyrazole-sensitive enzyme, pH 7.5
0.19
ethanol
10°C, pH 9.0
0.248
ethanol
40°C, pH 9.0
0.251
ethanol
recombinant His-tagged mutant C243S, pH 7.5, temperature not specified in the publication
0.34
ethanol
-
40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.43
ethanol
50°C, pH 9.0
0.45
ethanol
pH 9.0, 22°C, recombinant enzyme
0.48
ethanol
-
recombinant isozyme ADH1, pH 7.5, 25°C
0.5
ethanol
-
isoenzyme AA-ADH
0.63
ethanol
-
isoenzyme gamma2,gamma2
0.7
ethanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.7
ethanol
-
native enzyme, at 55°C
0.76
ethanol
-
isoenzyme 2
0.76
ethanol
-
isoenzyme BB-ADH
0.77
ethanol
isozyme ADH1C, at 21-23°C
0.8
ethanol
-
pyrazole-sensitive enzyme, pH 10.0
0.83
ethanol
-
recombinant isozyme ADH1, pH 10.5, 25°C
0.84
ethanol
-
isoenzyme beta2,beta2, 0.1 M sodium phosphate buffer, pH 7.5, 25°C
0.91
ethanol
wild-type, pH 8.0, 60°C
0.94
ethanol
-
isoenzyme beta2,beta2
1
ethanol
-
isoenzyme gamma1,gamma1
1.04
ethanol
60°C, pH 9.0
1.12
ethanol
-
in the presence of 0.025 mM Cu2+, at pH 9.5 and 37°C
1.33
ethanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
1.37
ethanol
-
isoenzyme 1
1.4
ethanol
-
isoenzyme ADH-3, pH 10.0 and pH 7.5
1.41
ethanol
-
isoenzyme 4
1.5
ethanol
-
isoenzyme alpha,alpha
1.5
ethanol
mutant C257L, pH 8.0, 60°C
1.6
ethanol
-
isoenzyme beta1,beta1
1.7
ethanol
-
isoenzyme III
1.7
ethanol
-
isoenzyme alpha,gamma1
1.7
ethanol
-
isoenzyme gamma2,gamma2
1.87
ethanol
-
isoenzyme 3
2.1
ethanol
-
in the presence of 0.025 mM Zn2+, at pH 9.5 and 37°C
2.3
ethanol
-
isozyme ADH I, pH 8.5, 25°C
2.46
ethanol
-
pH 7.3, 37°C
2.5
ethanol
Sporotrichum pulverulentum
-
-
2.95
ethanol
pH 7.5, 23°C
3.1
ethanol
at pH 9.0 in bis-Tris propane buffer
3.2
ethanol
-
isoenzyme gamma1,gamma1
3.2
ethanol
at pH 7.0 and 25°C
3.5
ethanol
-
isoenzyme III from leaf
3.6
ethanol
isozyme ADH4, at 21-23°C
4
ethanol
-
pyrazole-insensitive enzyme, pH 7.5
4
ethanol
-
wild-type enzyme Adh1
4.2
ethanol
-
isoenzyme alpha,alpha
4.6
ethanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.8
ethanol
-
enzyme form ADH I
5
ethanol
-
pyrazole-insensitive enzyme, pH 10.0
5.1
ethanol
-
pH 10.5, 25°C
5.7
ethanol
-
20°C, pH 9.0
5.7
ethanol
in 100 mM Tris-HCl, at pH 5.0 and 75°C
7.2
ethanol
-
isoenzyme II from leaf
7.3
ethanol
-
isozyme ADH II, pH 8.5, 25°C
7.75
ethanol
in 50 mM Tris-HCl, pH 8.0 at 25°C
8.1
ethanol
-
enzyme form ADH-3
8.56
ethanol
-
30°C, pH 9.0
9
ethanol
-
recombinant allozyme Ile308, pH 7.5, 25°C
9.03
ethanol
-
10°C, pH 9.0
10.4
ethanol
-
pH 9.0, 60°C, recombinant enzyme
10.6
ethanol
-
recombinant allozyme Val308, pH 7.5, 25°C
12
ethanol
-
pH 7.5, 25°C, Co-ADH
12.1
ethanol
-
40°C, pH 9.0
13
ethanol
-
in presence of 0.5 mM SDS
13.4
ethanol
-
mutant enzyme W54L
13.7
ethanol
pH 8.0, 60°C
14
ethanol
-
in presence of 3 mM SDS
17
ethanol
-
in presence of 1 mM SDS
17
ethanol
pH 7.0, temperature not specified in the publication
22
ethanol
-
mutant enzyme ADH1S-1108
24
ethanol
-
wild-type enzyme ADH1-1S
24.2
ethanol
-
pH 10.0, 50°C
26
ethanol
pH 7.0, temperature not specified in the publication
27
ethanol
-
enzyme form ADH II
27.5
ethanol
-
isoenzyme I from leaf
28
ethanol
-
isoenzyme TT-ADH
31
ethanol
pH 11.0, temperature not specified in the publication
33
ethanol
isozyme ADH2, at 21-23°C
36
ethanol
-
isoenzyme beta3,beta3, 0.1 M sodium phospahte buffer, pH 7.5, 25°C
39
ethanol
-
pH 7.5, 25°C, Cu-ADH
39.7
ethanol
pH 7.0, temperature not specified in the publication
41
ethanol
-
in presence of 15 mM SDS
49.5
ethanol
pH 7.0, temperature not specified in the publication
50
ethanol
-
in presence of 50 mM SDS
53
ethanol
-
pH 7.5, 25°C, native Zn-ADH
64
ethanol
Sporotrichum pulverulentum
-
-
66
ethanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
120
ethanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
184
ethanol
-
carboxymethylated enzyme, at 55°C
255
ethanol
-
recombinant isozyme ADH4, pH 10.5, 25°C
340
ethanol
-
isoenzyme ADH-1, pH 10.0
460
ethanol
-
pH 7.0, 30°C
460
ethanol
-
pH 7.0, 30°C
1625
ethanol
-
recombinant isozyme ADH4, pH 7.5, 25°C
5000
ethanol
-
isoenzyme ADH-1, pH 7.5
1
ethyl benzoylformate
-
65°C
4.2
ethyl benzoylformate
65°C, pH 5.0
5.3
ethyl benzoylformate
65°C, pH 5.5
50
ethylene glycol
-
isoenzyme alpha,gamma1
200
ethylene glycol
-
above
290
ethylene glycol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
4.46
formaldehyde
-
-
37.65
formaldehyde
-
pH 7.0, 30°C
50
formaldehyde
Megalodesulfovibrio gigas
-
-
0.13
furfural
-
mutant S110P/Y295C, pH 6.7, 30°C
18.8
furfural
-
wild-type, pH 6.7, 30°C
42.74
furfural
-
pH 7.0, 30°C
97.37
furfural
pH 7.0, 30°C, with NADH
2.58
glycerol
-
-
16.11
glycolaldehyde
pH 7.0, 30°C, with NADH
88.32
glycolaldehyde
-
pH 7.0, 30°C
0.0007
Hexanol
-
-
0.006
Hexanol
-
recombinant isozyme ADH4, pH 7.5, 25°C
0.085
Hexanol
-
recombinant isozyme ADH1, pH 7.5, 25°C
0.63
Hexanol
-
recombinant isozyme ADH4, pH 10.5, 25°C
1.9
Hexanol
-
recombinant isozyme ADH1, pH 10.5, 25°C
6.5
Hexanol
-
mutant enzyme W54L
13.8
Hexanol
-
wilde-type enzyme ADh1
0.71
isatin
pH 5.5, 65°C
1.26
iso-propanol
pH 7.5, 23°C
2.4
iso-propanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
0.26
Isobutyraldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.33
Isobutyraldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.76
Isobutyraldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
9
Isopropanol
-
-
20.3
Isopropanol
at pH 7.0 and 25°C
0.78
methanol
-
-
3.04
methanol
-
33°C, pH 8
12
methanol
-
pyrazole-sensitive enzyme, pH 7.5
14
methanol
-
pyrazole-sensitive enzyme, pH 10.0
150
methanol
-
isoenzyme alpha,gamma1
294.23
methanol
pH 7.5, 23°C
2.8
methyl o-chlorobenzoylformate
pH 5.5, 65°C
2.8
methyl o-chlorobenzoylformate
65°C, pH 5.5
0.13
N-benzyl-3-pyrrolidinone
-
pH 6.0
0.58
N-benzyl-3-pyrrolidinone
-
-
0.03
n-butanol
-
isoenzyme BB-ADH
0.07
n-butanol
-
isoenzyme AA-ADH
0.611
n-butanol
wild-type, pH 8.0, 60°C
2.6
n-butanol
-
isoenzyme TT-ADH
35.1
n-butanol
-
33°C, pH 8
0.02
n-Hexanol
-
isoenzyme TT-ADH
0.04
n-Hexanol
-
isoenzyme BB-ADH
0.26
n-Hexanol
-
isoenzyme TT-ADH
0.19
n-Propanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
7.49
n-Propanol
-
33°C, pH 8
0.001
NAD+
pH 8.0, 60°C
0.0074
NAD+
-
isoenzyme beta1,beta1
0.0074
NAD+
-
isoenzyme beta2,beta2
0.0079
NAD+
-
isoenzyme gamma1,gamma1
0.0087
NAD+
-
isoenzyme gamma2,gamma2
0.011
NAD+
-
oxidation of ethanol
0.013
NAD+
-
isoenzyme alpha,alpha
0.02
NAD+
cosubstrate butan-1-ol, pH 7.0, 30°C
0.028
NAD+
-
oxidation of propan-2-ol
0.037
NAD+
-
wild-type enzyme ADH1-1S
0.038
NAD+
-
mutant enzyme ADH1-1S1108
0.04
NAD+
-
isoenzyme ADH-2, pH 10.0 and pH 7.5
0.04
NAD+
-
isozyme ADH II, pH 8.5, 25°C
0.05
NAD+
-
isoenzyme ADH-1, pH 7.5
0.05
NAD+
-
pH 9.0, 60°C, recombinant enzyme
0.063
NAD+
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.063
NAD+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, wild-type enzyme
0.073
NAD+
-
enzyme form ADH I
0.074
NAD+
Megalodesulfovibrio gigas
-
oxidation of ethanol
0.074
NAD+
-
recombinant allozyme Val308, pH 7.5, 25°C
0.089
NAD+
cosubstrate 1-phenylethanol, pH 9.0, 70°C
0.097
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsC
0.1
NAD+
-
isoenzyme ADH-3
0.1
NAD+
at pH 9.0 in bis-Tris propane buffer
0.1
NAD+
-
pH 8.8, 45°C, mutant enzyme G211S
0.11
NAD+
-
enzyme form ADH II
0.12
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsA
0.127
NAD+
cosubstrate 2,3-butanediol, pH 9.0, 70°C
0.13
NAD+
in 200 mM bicine, pH 9.0, at 60°C
0.17
NAD+
Sporotrichum pulverulentum
-
-
0.18
NAD+
-
isoenzyme beta1,beta1
0.2
NAD+
-
isoenzyme ADH-1, pH 7.5
0.2
NAD+
-
in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.2
NAD+
-
native enzyme, at 55°C
0.2
NAD+
mutant W87A, pH 7.0, 30°C
0.23
NAD+
-
oxidation of ethanol
0.23
NAD+
-
pH 8.8, 45°C, wild-type enzyme
0.239
NAD+
wild-type, pH 8.0, 60°C
0.24
NAD+
-
pH 10.5, 65°C
0.25
NAD+
-
isoenzyme ADH-1, pH 10.0
0.29
NAD+
pH 11.0, temperature not specified in the publication
0.29
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsG
0.39
NAD+
at pH 7.0 and 25°C
0.46
NAD+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, mutant enzyme K249G/H255R
0.48
NAD+
-
pH 8.8, 45°C, mutant enzyme G211C
0.5
NAD+
-
carboxymethylated enzyme, at 55°C
0.5
NAD+
-
wild type enzyme, at 65°C, in the presence of benzyl alcohol
0.5
NAD+
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.5
NAD+
mutant A25Y, pH 7.0, 30°C
0.5
NAD+
mutant Y25A/W49F/W167Y, 30°C, pH not specified in the publication
0.9
NAD+
-
reduction of ethanol
0.9
NAD+
mutant Y25A/W49F/W87F, 30°C, pH not specified in the publication
1
NAD+
mutant Y25A, 30°C, pH not specified in the publication
1
NAD+
mutant Y25A, pH 7.0, 30°C
1.1
NAD+
wild-type, pH 7.0, 30°C
1.1
NAD+
wild-type, 30°C, pH not specified in the publication
1.2
NAD+
-
isozyme ADH I, pH 8.5, 25°C
1.3
NAD+
mutant W49F/W87F, 30°C, pH not specified in the publication
1.51
NAD+
cosubstrate ethanol, pH 8, 60°C
1.6
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsS
1.65
NAD+
-
pH 10.5, 25°C
1.8
NAD+
mutant W49F/W167Y, 30°C, pH not specified in the publication
4
NAD+
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
7.4
NAD+
-
isoenzyme beta1,beta1, 0.1 M sodium phosphate buffer, pH 7.5, at 25 °C
8.3
NAD+
mutant W49F/W167Y/V260A, 30°C, pH not specified in the publication
9.7
NAD+
mutant Y25A/W49F/W167Y/V260A, 30°C, pH not specified in the publication
10
NAD+
mutant V260A, 30°C, pH not specified in the publication
10.2
NAD+
mutant W49F/W87F/V260A, 30°C, pH not specified in the publication
12.4
NAD+
-
mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol
14.8
NAD+
mutant Y25A/W49F/W87F/V260A, 30°C, pH not specified in the publication
30
NAD+
-
isoenzyme II from leaf
31
NAD+
-
isoenzyme III from leaf
40
NAD+
-
enzyme from liver
50
NAD+
Km above 50 mM, mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
60
NAD+
-
enzyme from caecum
73
NAD+
-
isoenzyme I from leaf
180
NAD+
-
isoenzyme beta2,beta2, 0.1 M sodium phosphate buffer, pH 7.5, 25°C
550
NAD+
-
reduction of propan-2-ol
710
NAD+
-
isoenzyme beta3,beta3, 0.1 M sodium phospahte buffer, pH 7.5, 25°C
0.0004
NADH
pH 8.0, 60°C
0.0017
NADH
-
isoenzyme ADH-2, pH 7.5
0.0035
NADH
-
pH 10.5, 65°C
0.0051
NADH
-
reduction of octanal
0.0064
NADH
-
isoenzyme beta1,beta1
0.007
NADH
-
isoenzyme gamma1,gamma1
0.008
NADH
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.01
NADH
in 200 mM bicine, pH 6.0, at 60°C
0.01
NADH
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.011
NADH
-
isoenzyme alpha,alpha
0.012
NADH
-
enzyme form ADH II
0.013
NADH
-
wild type enzyme, at 65°C, in the presence of benzaldehyde
0.016
NADH
-
pH 7.7, 60°C
0.02
NADH
Megalodesulfovibrio gigas
-
reduction of acetaldehyde
0.021
NADH
Sporotrichum pulverulentum
-
-
0.027
NADH
-
enzyme form ADH I
0.03
NADH
-
isozyme ADH II, pH 6.0, 25°C
0.03
NADH
-
in 50 mM Tris-HCl (pH 7.5) containing 0.25 mM benzaldehyde, at 65°C
0.03
NADH
-
native enzyme, at 55°C
0.038
NADH
-
wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+
0.04
NADH
-
carboxymethylated enzyme, at 55°C
0.042
NADH
-
mutant enzyme ADH1-1S1108
0.048
NADH
-
25°C, pH 7.0
0.05
NADH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, mutant enzyme K249G/H255R
0.062
NADH
-
alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+
0.071
NADH
pH 6.0, temperature not specified in the publication
0.072
NADH
wild-type, pH 8.0, 60°C
0.085
NADH
-
wild-type enzyme ADH1-1S
0.09
NADH
cosubstrate butanal, pH 7.0, 30°C
0.105
NADH
-
isoenzyme beta2,beta2
0.105
NADH
cosubstrate acetoin, pH 6.0, 70°C
0.113
NADH
cosubstrate acetoin, pH 6.0, 70°C
0.19
NADH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, wild-type enzyme
0.2
NADH
-
isozyme ADH I, pH 5.5, 25°C
0.2
NADH
-
pH 8.0, 60°C, recombinant enzyme
0.21
NADH
-
mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde
0.75
NADH
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.4
NADH
cosubstrate acetaldehyde, pH 8, 60°C
6.4
NADH
-
isoenzyme beta1,beta1, 0.1 M sodium phosphate buffer, pH 7.5, at 25 °C
25
NADH
-
isoenzyme III from leaf
70
NADH
-
isoenzyme II from leaf
105
NADH
-
isoenzyme beta2,beta2, 0.1 M sodium phosphate buffer, pH 7.5, 25°C
121
NADH
-
isoenzyme I from leaf
260
NADH
-
isoenzyme beta3,beta3, 0.1 M sodium phospahte buffer, pH 7.5, 25°C
0.0051
NADP+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, wild-type enzyme
0.066
NADP+
cosubstrate 1-phenylethanol, pH 9.0, 70°C
0.078
NADP+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, mutant enzyme K249G/H255R
0.113
NADP+
cosubstrate 2,3-butanediol, pH 9.0, 70°C
0.28
NADP+
cosubstrate ethanol, pH 8, 60°C
7.4
NADP+
-
pH 8.8, 45°C, wild-type enzyme
15
NADP+
-
pH 8.8, 45°C, mutant enzyme G211S
25
NADP+
-
pH 8.8, 45°C, mutant enzyme G211C
33
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsG
66
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsS
120
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsC
360
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsA
0.033
NADPH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, mutant enzyme K249G/H255R
0.28
NADPH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, wild-type enzyme
0.043
octanal
-
enzyme form ADH-2
0.3
octanal
-
isoenzyme ADH-1, pH 7.5
3.5
octanal
-
isoenzyme ADH-1, pH 7.5
9.6
octanal
isozyme ADH3, at 21-23°C
0.0014
Octanol
-
-
0.007
Octanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.009
Octanol
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.016
Octanol
-
recombinant allozyme Val308, pH 7.5, 25°C
0.08
Octanol
-
isoenzyme alpha,gamma1
0.6
Octanol
pH and temperature not specified in the publication
1.6
Octanol
-
isoenzyme ADH-2, pH 7.5
2.9
p-Methoxybenzaldehyde
-
isozyme ADH1, in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
3.4
p-Methoxybenzaldehyde
in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
5.9
p-Methoxybenzaldehyde
-
isozyme ADH2, in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
6.9
p-methoxybenzyl alcohol
-
isozyme ADH2, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
11
p-methoxybenzyl alcohol
-
isozyme ADH1, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
22
p-methoxybenzyl alcohol
in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
0.2
pentan-1-ol
-
enzyme form ADH-3
0.012
Pentanol
-
pyrazole-sensitive enzyme, pH 7.5
0.018
Pentanol
-
pyrazole-sensitive enzyme, pH 10.0
0.09
Pentanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
11.8
Pentanol
-
mutant enzyme W54L
56.2
Pentanol
-
wilde-type enzyme ADh1
0.19
phenylacetaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.26
phenylacetaldehyde
-
-
0.33
phenylacetaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.91
phenylacetaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
24.45
phenylglyoxylic acid
-
soluble enzyme
37.55
phenylglyoxylic acid
-
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde
0.019
propan-1-ol
-
-
0.28
propan-1-ol
Megalodesulfovibrio gigas
-
-
0.5
propan-2-ol
-
-
64
propan-2-ol
Sporotrichum pulverulentum
-
-
193
propan-2-ol
-
mutant enzyme W54L
0.59
propanal
-
33°C, pH 7
41.4
propanal
-
pH 7.0, 30°C
0.91
Propanol
-
-
13.6
Propanol
-
wilde-type enzyme ADh1
43.9
Propanol
-
mutant enzyme W54L
0.011
retinol
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.012
retinol
-
recombinant allozyme Val308, pH 7.5, 25°C
0.009
trans-cinnamaldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.09
trans-cinnamaldehyde
mutant enzyme W95L,, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.23
trans-cinnamaldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.17
Valeraldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.28
Valeraldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
2
Valeraldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.034
Vanillyl alcohol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
pH-dependence of Km-value
-
additional information
additional information
-
kinetics of ethanol oxidation
-
additional information
additional information
-
kinetics of ethanol oxidation
-
additional information
additional information
-
kinetics of native and modified enzyme with coenzyme analogues
-
additional information
additional information
-
Km-values of active-site Co(II)substituted enzyme
-
additional information
additional information
-
Km values for the class I isoenzymes with the substrates ethanol, methanol, ethylene glycol, benzyl alcohol, octanol, cyclohexanol and 16-hydroxyhexadecanoic acid
-
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
detailed kinetic mechanism, steady-state kinetics for wild-type and mutant enzymes, investigation of pH-dependency
-
additional information
additional information
-
detailed kinetic mechanism, steady-state kinetics for wild-type and mutant enzymes, investigation of pH-dependency
-
additional information
additional information
-
detailed kinetics, computational analysis of the reaction mechanism
-
additional information
additional information
-
Km for isozymes ADH1, and ADH4 for all retinoid substrates in forward and reverse reaction
-
additional information
additional information
-
Km for isozymes ADH1B1, ADH1B2, and ADH4 for all retinoid substrates in forward and reverse reaction
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
steady-state kinetics, kinetic mechanism
-
additional information
additional information
-
steady-state kinetics, MW 25 kDa
-
additional information
additional information
-
wild-type and mutant forms of the 3 isozymes, steady-state kinetics, detailed kinetic analysis, at different pH values and temperatures
-
additional information
additional information
-
effects of tert-butanol, butyramide, valeramide and capronamide on KM-value for ethanol
-
additional information
additional information
-
kinetic data füor wild-type enzyme and chimeric enzyme created by insertion of an RTX domain from the adenylate cyclase of Bordetella pertussis into a loop near the catalytic active site of the thermostable alcohol dehydrogenase D (AdhD) from Pyrococcus furiosus
-
additional information
additional information
models for the Michaelis complexes with NAD+-alcohol and NADH-aldehyde are proposed
-
additional information
additional information
-
pre-steady-state and Michaelis-Menten steady-state kinetics, molecular dynamics simulations of enzyme-substrate interactions in the Michaelis complexes of wild-type ADH-A and Y294F/W295A double mutant. Interdependency between substrate/product and the cofactor in the ternary complex is determined, which directly affects the NADH dissociation rates, therefore, this substrate-coenzyme crosstalk plays a direct role in determining the turnover rates. Model of the kinetic mechanism of ADH-A, overview
-
additional information
additional information
reaction kinetics at different pH values, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.