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The enzyme appears in viruses and cellular organisms
Synonyms
5-keto-D-fructose reductase (NADP+), 5-ketofructose reductase (NADP), 5-ketofructose reductase [NADP+], D-(-)fructose:(NADP+) 5-oxidoreductase, fructose-5-(nicotinamide adenine dinucleotide phosphate) dehydrogenase,
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5-keto-D-fructose reductase (NADP+)
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5-ketofructose reductase (NADP)
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5-ketofructose reductase [NADP+]
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D-(-)fructose:(NADP+) 5-oxidoreductase
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fructose-5-(nicotinamide adenine dinucleotide phosphate) dehydrogenase
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D-fructose + NADP+ = 5-dehydro-D-fructose + NADPH + H+
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D-fructose:NADP+ 5-oxidoreductase
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5-dehydro-D-fructose + NADH + H+
D-fructose + NAD+
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r
5-keto-D-fructose 1-phosphate + NADPH + H+
D-fructose 1-phosphate + NADP+
D-arabinose + NADP+
? + NADPH
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5fold lower activity than for D-fructose
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D-fructose + NAD+
5-dehydro-D-fructose + NADH + H+
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r
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
5-keto-D-fructose 1-phosphate + NADPH + H+
D-fructose 1-phosphate + NADP+
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16% of the rate compared to 5-keto-D-fructose
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5-keto-D-fructose 1-phosphate + NADPH + H+
D-fructose 1-phosphate + NADP+
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16% of the rate compared to 5-keto-D-fructose
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D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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highly specific for D-fructose
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r
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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predominant formation of D-fructose
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r
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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predominant formation of D-fructose
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r
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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highly specific for D-fructose
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r
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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highly specific for D-fructose
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r
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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predominant reduction of 5-keto-D-fructose
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r
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D-fructose + NADP+
5-dehydro-D-fructose + NADPH
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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highly specific for D-fructose
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r
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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predominant formation of D-fructose
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r
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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predominant formation of D-fructose
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r
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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highly specific for D-fructose
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r
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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highly specific for D-fructose
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r
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
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predominant reduction of 5-keto-D-fructose
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r
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NADH
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substitutes for NADPH
NADPH
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highly specific
NADPH
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cannot be replaced by NADH
NADPH
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oxidized and reduced cofactors bind to the same site of the enzyme
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NADP+
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competitive inhibition of D-fructose synthesis, non-competitive inhibition of 5-keto-fructose synthesis
NADPH
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competitive inhibition of D-fructose oxidation
D-fructose
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non-competitive inhibition
D-fructose
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inhibition of 5-keto-D-frucotse reduction, Ki: 82 mM
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4.5 - 12.7
5-keto-D-fructose
4.2
5-Keto-D-fructose 1-phosphate
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4.5
5-keto-D-fructose
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4.5
5-keto-D-fructose
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12.7
5-keto-D-fructose
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9.1
D-fructose
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0.053
NADP+
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0.002
NADPH
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3 - 5
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D-fructose oxidation
620
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5-keto-D-fructose reduction
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7
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reduction of 5-keto-D-fructose
7.4
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NADPH oxidation in Tris-HCl buffer
8.5 - 10
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D-fructose oxidation
9.5
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oxidation of D-fructose
6.5
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NADPH oxidation in phosphate buffer
6.5
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5-keto-D-fructose reduction
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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DHF5_ERWCI
39
0
4314
Swiss-Prot
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25000
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4 * 25000, SDS-PAGE
40000
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1 * 40000, SDS-PAGE
50000
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gel filtration, sedimentation equilibrium measurement
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monomer
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1 * 40000, SDS-PAGE
tetramer
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4 * 25000, SDS-PAGE
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ammonium sulfate precipitation
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4 - 8
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stable between
285815
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-20°C, 30 mM Tris-HCl, pH 7.8, 3 months
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4°C, stable for 3-4 weeks
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high purity to crystalline form, chromatography techniques
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to homogeneity, chromatography techniques
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Schrimsher, J.L.; Wingfield, P.T.; Bernard, A.; Mattaliano, R.; Payton, M.A.
Purification and characterization of 5-ketofructose reductase from Erwinia citreus
Biochem. J.
253
511-516
1988
Erwinia sp.
brenda
Ameyama, M.; Matsushita, K.; Shinagawa, E.; Adachi, O.
5-keto-D-Fructose reductase of Gluconobacter industrius: Purification, crystallization and properties
Agric. Biol. Chem.
45
863-869
1981
Gluconobacter oxydans
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brenda
Englard, S.; Avigad, G.
5-keto-D-Fructose reductase from Gluconobacter cerinus
Methods Enzymol.
41B
127-131
1978
Gluconobacter cerinus
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brenda
Avigad, G.; Englard, S.; Pifko, S.
5-Keto-D-fructose. IV. A specific reduced nicotinamide adenine dinucleotide phosphate-linked reductase from Gluconobacter cerinus
J. Biol. Chem.
241
373-378
1966
Gluconobacter cerinus
brenda
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1.1.1.124 fructose 5-dehydrogenase (NADP+)
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