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Information on EC 1.1.1.179 - D-xylose 1-dehydrogenase (NADP+, D-xylono-1,5-lactone-forming)
for references in articles please use BRENDA:EC1.1.1.179
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EC Tree 1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.1.1 With NAD+ or NADP+ as acceptor
1.1.1.179 D-xylose 1-dehydrogenase (NADP+, D-xylono-1,5-lactone-forming)
IUBMB Comments
The enzyme, characterized from pig arterial vessels and eye lens, also acts, more slowly, on L-arabinose and D-ribose. cf. EC 1.1.1.424, D-xylose 1-dehydrogenase (NADP+, D-xylono-1,4-lactone-forming).
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
nadp+-dependent d-xylose dehydrogenase, more
top
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D-xylose (nicotinamide adenine dinucleotide phosphate) dehydrogenase
-
-
ambiguous
-
D-xylose 1-dehydrogenase (NADP)
-
-
ambiguous
-
D-xylose-NADP dehydrogenase
-
-
ambiguous
-
D-xylose:NADP+ oxidoreductase
-
-
ambiguous
-
NADP+-dependent D-xylose dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-xylose + NADP+ = D-xylono-1,5-lactone + NADPH + H+
also acts, more slowly, on L-arabinose and D-ribose
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
-
-
-
-
oxidation
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
D-xylose:NADP+ 1-oxidoreductase (D-xylono-1,5-lactone-forming)
The enzyme, characterized from pig arterial vessels and eye lens, also acts, more slowly, on L-arabinose and D-ribose. cf. EC 1.1.1.424, D-xylose 1-dehydrogenase (NADP+, D-xylono-1,4-lactone-forming).
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CAS REGISTRY NUMBER
COMMENTARY
83534-37-6
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
camphorquinone + NADPH
(1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+
-
Substrates: -
Products: -
Products: -
?
D-arabinose + NADP+
D-arabinono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-glucose + NAD(P)+
D-gluconolactone + NAD(P)H
Substrates: -
Products: -
Products: -
?
D-glucose + NADP+
D-gluconolactone + NADPH + H+
Substrates: reaction is catalyzed with 70fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP*
Products: -
Products: -
?
D-ribose + NADP+
D-ribonolactone + NADPH + H+
Substrates: reaction is catalyzed with 1.8fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP*
Products: -
Products: -
?
D-xylose + NAD(P)+
D-xylonolactone + NAD(P)H
Substrates: is highly specific for D-xylose
Products: -
Products: -
?
D-xylose + NAD+
D-xylonolactone + NADH + H+
Substrates: Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+
Products: -
Products: -
?
L-arabinose + NADP+
L-arabinono-1,4-lactone + NADPH + H+
-
Substrates: 44% of the activity with D-xylose
Products: -
Products: -
?
2-deoxy-D-glucose + NADP+
2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
2-deoxy-D-glucose + NADP+
2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
2-deoxy-D-glucose + NADP+
2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-galactose + NADP+
D-galactono-1,5-lactone + NADPH
-
Substrates: -
Products: -
Products: -
?
D-galactose + NADP+
D-galactono-1,5-lactone + NADPH
-
Substrates: -
Products: -
Products: -
?
D-galactose + NADP+
D-galactono-1,5-lactone + NADPH
A8BT09
Substrates: -
Products: -
Products: -
?
D-glucose + NADP+
D-glucono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-glucose + NADP+
D-glucono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-glucose + NADP+
D-glucono-1,5-lactone + NADPH + H+
Substrates: 20% of the activity with D-xylose
Products: -
Products: -
?
D-glucose + NADP+
D-glucono-1,5-lactone + NADPH + H+
Substrates: 20% of the activity with D-xylose
Products: -
Products: -
?
D-glucose + NADP+
D-glucono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-glucose + NADP+
D-glucono-1,5-lactone + NADPH + H+
A8BT09
Substrates: -
Products: -
Products: -
?
D-mannose + NADP+
D-mannono-1,5-lactone + NADPH
-
Substrates: -
Products: -
Products: -
?
D-mannose + NADP+
D-mannono-1,5-lactone + NADPH
-
Substrates: -
Products: -
Products: -
?
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
Substrates: -
Products: -
Products: -
r
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
-
Substrates: very low activity compared to NADP+
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
Substrates: initial step in xylose degradation
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
Substrates: initial step in xylose degradation
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
Substrates: enzyme has dehydrogenase and reductase activities, enzyme is identical to dihydrodiol dehydrogenase
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
A8BT09
Substrates: best substrate
Products: -
Products: -
?
D-xylose + NADP+
D-xylonolactone + NADPH + H+
Substrates: initial step in xylose degradation
Products: -
Products: -
?
D-xylose + NADP+
D-xylonolactone + NADPH + H+
Substrates: Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+
Products: -
Products: -
?
L-arabinose + NADP+
L-arabinono-1,4-lactone + NADPH
-
Substrates: -
Products: -
Products: -
?
L-arabinose + NADP+
L-arabinono-1,4-lactone + NADPH
-
Substrates: -
Products: -
Products: -
?
L-arabinose + NADP+
L-arabinono-1,4-lactone + NADPH
A8BT09
Substrates: -
Products: -
Products: -
?
trans-benzene dihydrodiol + NADP+
?
-
Substrates: dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20
Products: -
Products: -
?
trans-benzene dihydrodiol + NADP+
?
-
Substrates: dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20
Products: -
Products: -
?
trans-benzene dihydrodiol + NADP+
?
-
Substrates: dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20
Products: -
Products: -
?
trans-benzene dihydrodiol + NADP+
?
-
Substrates: dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20
Products: -
Products: -
?
trans-naphthalene dihydrodiol + NADP+
?
-
Substrates: dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20
Products: -
Products: -
?
trans-naphthalene dihydrodiol + NADP+
?
-
Substrates: dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20
Products: -
Products: -
?
trans-naphthalene dihydrodiol + NADP+
?
-
Substrates: dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20
Products: -
Products: -
?
trans-naphthalene dihydrodiol + NADP+
?
-
Substrates: dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20
Products: -
Products: -
?
additional information
?
-
-
Substrates: D-arabinose, D-ribose, D-glucose and D-galactose are oxidized at 10-30% the rate of D-xylose
Products: -
Products: -
?
additional information
?
-
-
Substrates: catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20
Products: -
Products: -
?
additional information
?
-
-
Substrates: D-arabinose, D-ribose, D-glucose and D-galactose are oxidized at 10-30% the rate of D-xylose
Products: -
Products: -
?
additional information
?
-
Substrates: no significant oxidation with L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
Products: -
Products: -
?
additional information
?
-
Substrates: no significant oxidation with L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
Products: -
Products: -
?
additional information
?
-
-
Substrates: catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20
Products: -
Products: -
?
additional information
?
-
Substrates: D-xylose dehydrogenase catalyzes the NADP+-linked oxidation of dihydrodiols of aromatic hydrocarbons to their corresponding catechols and is regarded as a toxication enzyme in the metabolism of carcinogenic polycyclic aromatic hydrocarbons because oxidation by the enzyme yields reactive and redox-active ortho-quinones and reactive oxygen species, broad substrate specificity indicates additional roles of the enzyme in the metabolism of endogenous and xenobiotic carbonyl compounds and the prevention of the development of glycation
Products: -
Products: -
?
additional information
?
-
-
Substrates: catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20
Products: -
Products: -
?
additional information
?
-
-
Substrates: catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrates: D-arabinose, D-glucose, D-mannose, D-galactose
Products: -
Products: -
?
additional information
?
-
-
Substrates: catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20
Products: -
Products: -
?
additional information
?
-
-
Substrates: at concentrations of up to 50 mM of L-xylose, D-ribose, D-arabinose, L-arabinose, D-glucose, D-galactose or D-fructose, the enzyme produces little NADPH and the specific activity toward these substrates are below 5% of the activity recorded with D-xylose
Products: -
Products: -
?
additional information
?
-
-
Substrates: D-arabinose, D-ribose, D-glucose and D-galactose are oxidized at 10-30% the rate of D-xylose
Products: -
Products: -
?
additional information
?
-
A8BT09
Substrates: no or poor activity with D-arabinose, D-mannose, L-rhamnose, D-lyxose, D-fructose, D-glyceraldehyde, and DL-glyceraldehyde
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-xylose + NADP+
D-xylonolactone + NADPH + H+
Substrates: initial step in xylose degradation
Products: -
Products: -
?
L-arabinose + NADP+
L-arabinono-1,4-lactone + NADPH + H+
-
Substrates: 44% of the activity with D-xylose
Products: -
Products: -
?
additional information
?
-
Substrates: D-xylose dehydrogenase catalyzes the NADP+-linked oxidation of dihydrodiols of aromatic hydrocarbons to their corresponding catechols and is regarded as a toxication enzyme in the metabolism of carcinogenic polycyclic aromatic hydrocarbons because oxidation by the enzyme yields reactive and redox-active ortho-quinones and reactive oxygen species, broad substrate specificity indicates additional roles of the enzyme in the metabolism of endogenous and xenobiotic carbonyl compounds and the prevention of the development of glycation
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
Substrates: initial step in xylose degradation
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
Substrates: initial step in xylose degradation
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
Substrates: enzyme has dehydrogenase and reductase activities, enzyme is identical to dihydrodiol dehydrogenase
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-xylose + NADP+
D-xylono-1,5-lactone + NADPH + H+
A8BT09
Substrates: best substrate
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NADP+
preferred electron acceptor. Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2.4
D-xylose
oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
2.9
D-xylose
oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
3.4
D-xylose
oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
4.4
D-xylose
oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
8 - 9
D-xylose
oxidation reaction of mutant F279A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
11
D-xylose
oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
14
D-xylose
oxidation reaction of mutant W254Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
15
D-xylose
oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
36
D-xylose
oxidation reaction of mutant F154A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
41
D-xylose
oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
65
D-xylose
oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
67
D-xylose
oxidation reaction of mutant W125Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
90
D-xylose
oxidation reaction of mutant W254A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose
additional information
additional information
-
phosphate buffer increases the Km-value, especially for mutant H79Q, compared with Tris buffer
-
additional information
additional information
A8BT09
kinetics, recombinant enzyme
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.4
D-xylose
oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose
0.45
D-xylose
oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose
0.5
D-xylose
oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose
0.9
D-xylose
oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose
1
D-xylose
oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose
1.2
D-xylose
oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose
1.7
D-xylose
oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose
2.6
D-xylose
oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10
7.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
30