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Information on EC 1.1.1.278 - 3beta-hydroxy-5alpha-steroid dehydrogenase
for references in articles please use BRENDA:EC1.1.1.278
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EC Tree 1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.1.1 With NAD+ or NADP+ as acceptor
1.1.1.278 3beta-hydroxy-5alpha-steroid dehydrogenase
The enzyme appears in viruses and cellular organisms
Reaction Schemes
showSynonyms
3beta-hydroxysteroid oxidoreductase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
3beta-HSD/isomerase
3beta-hydroxy-steroid dehydrogenase/DELTA5-DELTA4 isomerase
3beta-hydroxysteroid-5alpha-oxidoreductase
dehydrogenase, 3(17)beta-hydroxy steroid
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-
-
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additional information
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3beta-hydroxysteroid oxidoreductase is exclusively associated with the 5alpha-progesterone reductase in microsomes
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3beta-hydroxy-5alpha-pregnane-20-one + NADP+ = 5alpha-pregnan-3,20-dione + NADPH + H+
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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-
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redox reaction
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-
-
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reduction
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
digitoxigenin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
3beta-hydroxy-5alpha-steroid:NADP+ 3-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY
9015-81-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
Substrates: progesterone is no substrate
Products: -
Products: -
?
3beta-hydroxy-16alpha,17alpha-epoxypregn-5-en-20-one + NADP+
?
-
Substrates: is metabolized through the putative 3beta-HSD/isomerase pathway
Products: -
Products: -
?
3beta-hydroxy-16alpha,17alpha-epoxypregn-5-en-20-one + NADP+
?
-
Substrates: is metabolized through the putative 3beta-HSD/isomerase pathway
Products: -
Products: -
?
5alpha-pregnan-3,20-dione + NAD(P)H
3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NAD(P)H
3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+
-
Substrates: -
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
r
5alpha-pregnan-3,20-dione + NAD(P)H
3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+
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Substrates: -
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
r
5alpha-pregnan-3,20-dione + NAD(P)H
3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NAD(P)H
3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NAD(P)H
3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NAD(P)H
3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+
-
Substrates: -
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
r
5alpha-pregnan-3,20-dione + NAD(P)H
3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+
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Substrates: low activity
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
r
5alpha-pregnan-3,20-dione + NAD(P)H
3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+
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Substrates: activity below detection limit of 0.1 fmol/mg protein/min
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
r
5alpha-pregnan-3,20-dione + NAD(P)H
3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+
-
Substrates: -
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
Products: i.e. 5alpha-pregnan-3beta-ol-20-one
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: low activity
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: biosynthetic pathway, overview
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: -
Products: -
Products: -
?
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: biosynthetic pathway, overview
Products: -
Products: -
r
dehydroepiandrosterone + NADP+
?
-
Substrates: can also enter a minor hydroxylation pathway
Products: -
Products: -
?
dehydroepiandrosterone + NADP+
?
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Substrates: can also enter a minor hydroxylation pathway
Products: -
Products: -
?
pregnenolone + NADP+
?
-
Substrates: is metabolized through the putative 3beta-HSD/isomerase pathway
Products: -
Products: -
?
pregnenolone + NADP+
?
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Substrates: is metabolized through the putative 3beta-HSD/isomerase pathway
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: -
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: low activity
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: biosynthetic pathway, overview
Products: -
Products: -
r
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
Substrates: biosynthetic pathway, overview
Products: -
Products: -
r
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
presence of C-7 allylic hydroxylation, in either epimeric form, inhibits 3beta-HSD/isomerase activity
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
3beta-HSD/isomerase activity only occurs following lactonization of the steroidal ring-D
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
activity is determined as progesterone-metabolizing activity including steroid 5alpha-reductase and 3beta-hydroxysteroid dehydrogenase activity
additional information
-
activity below detection limit of 0.1 fmol/mg protein/min
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
activity below detection limit of 0.1 fmol/mg protein/min
brenda
additional information
-
only male rats, no activity in tissues of pregnant and non-pregnant female rats
brenda
-
only male rats, no activity in tissues of pregnant and non-pregnant female rats
-
brenda
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only male rats, no activity in tissues of pregnant and non-pregnant female rats
brenda
-
only male rats, no activity in tissues of pregnant and non-pregnant female rats
-
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lindemann, P.; Luckner, M.
Biosynthesis of pregnane derivatives in somatic embryos of Digitalis lanata
Phytochemistry
46
507-513
1997
Digitalis lanata, Digitalis lanata VIII
-
brenda
Giacomini, M.; Wright, F.
The effects of progesterone and pregnanedione on the reductive metabolism of dihydrotestosterone in human skin
J. Steroid Biochem.
13
645-651
1980
Homo sapiens
brenda
Ichihara, K.; Tanaka, C.
Progesterone metabolism in the gastric mucosa microsomes of guinea pig
J. Steroid Biochem.
32
835-840
1989
Cavia porcellus, Oryctolagus cuniculus, Rattus norvegicus
brenda
Warneck, H.M.; Seitz, H.U.
3beta-Hydroxysteroid oxidoreductase in suspension cultures of Digitalis lanata EHRH
Z. Naturforsch. C
45
963-972
1990
Digitalis lanata
brenda
Laine, M.; Ojanotko-Harri, A.
Progesterone metabolism by major salivary glands of rat. II. Parotid gland
J. Steroid Biochem. Mol. Biol.
37
605-611
1990
Rattus norvegicus, Rattus norvegicus Turku
brenda
Stuerenburg, H.J.; Fries, U.; Iglauer, F.; Kunze, K.
Effect of age on synthesis of the GABAergic steroids 5-alpha-pregnane-3,20-dione and 5-alpha-pregnane-3-alpha-ol-20-one in rat cortex in vitro
J. Neural Transm.
104
249-257
1997
Rattus norvegicus
brenda
Wiebe, J.P.; Boushy, D.; Wolfe, M.
Synthesis, metabolism and levels of the neuroactive steroid, 3alpha-hydroxy-4-pregnen-20-one (3alphaHP), in rat pituitaries
Brain Res.
764
158-166
1997
Rattus norvegicus
brenda
Petralia, S.M.; Jahagirdar, V.; Frye, C.A.
Inhibiting biosynthesis and/or metabolism of progestins in the ventral tegmental area attenuates lordosis of rats in behavioural oestrus
J. Neuroendocrinol.
17
545-552
2005
Rattus norvegicus
brenda
Hunter, A.C.; Coyle, E.; Morse, F.; Dedi, C.; Dodd, H.T.; Koussoroplis, S.J.
Transformation of 5-ene steroids by the fungus Aspergillus tamarii KITA: Mixed molecular fate in lactonization and hydroxylation pathways with identification of a putative 3beta-hydroxy-steroid dehydrogenase/DELTA(5)-DELTA(4) isomerase pathway
Biochim. Biophys. Acta
1791
110-117
2008
Aspergillus tamarii
brenda
EXTERNAL LINKS (specific for EC-Number 1.1.1.278)
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