Information on EC 1.1.1.366 - L-idonate 5-dehydrogenase (NAD+)

for references in articles please use BRENDA:EC1.1.1.366
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The expected taxonomic range for this enzyme is: Vitis vinifera

EC NUMBER
COMMENTARY hide
1.1.1.366
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RECOMMENDED NAME
GeneOntology No.
L-idonate 5-dehydrogenase (NAD+)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-idonate + NAD+ = 5-dehydro-D-gluconate + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-ascorbate degradation IV
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ketogluconate metabolism
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SYSTEMATIC NAME
IUBMB Comments
L-idonate:NAD+ oxidoreductase
Involved in the catabolism of ascorbate (vitamin C) to tartrate. No activity is observed with NADP+ (cf. EC 1.1.1.264, L-idonate 5-dehydrogenase).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
enzyme LidnDH catalyzes the conversion of L-idonate to 5-keto D-gluconic acid in plant and is proposed to be a rate-limiting step in biosynthesis of tartaric acid from ascorbic acid
additional information
cultivar Cabernet Sauvignon transcriptome analysis dependent on regional parameters of six different samples, overview. Two unigenes encoding L-idonate dehydrogenase (LidnDH) are detected from the assembled transcriptome. One is expressed higher in Changli region berries than in Gaotai region berries at E-L 31 stage,while the other is expressed higher at E-L 38 stage in Gaotai region berries
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-idonate + NAD+
5-dehydro-D-gluconate + NADH + H+
show the reaction diagram
sorbitol + NAD+
?
show the reaction diagram
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when sorbitol is tested as a substrate, NAD+ reduction occurs but at a very low rate
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r
additional information
?
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no activity with NADPH and NADP+
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-idonate + NAD+
5-dehydro-D-gluconate + NADH + H+
show the reaction diagram
sorbitol + NAD+
?
show the reaction diagram
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when sorbitol is tested as a substrate, NAD+ reduction occurs but at a very low rate
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r
additional information
?
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no activity with NADPH and NADP+
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
grape berries from regions Changli (CL) and Gaotai (GT)in eastern and western China,respectively. Some genes required for carbohydrate metabolism, such as hexose transporter, L-idonate dehydrogenase, and phosphoenolpyruvate carboxylase, are significantly upregulated in the CL berries in relation to the GT berries, which positively correlated with the sugar and organic acid accumulations
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
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x * 40000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 40000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) pLysS cells
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gene LidnDH, DNA library construction from a single pool of six samples, DNA sequence determination and comparisons, quantitative real-time PCR enzyme expression analysis in different regional samples of cv. Cabernet Sauvignon berries
IdnDh gene, quantitative expression analysis on transcriptional and translational levels in fruits of different stages