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12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
3,7-dioxo-5beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD+
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Substrates: -
Products: -
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3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
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Substrates: i.e. isochenodeoxycholic acid
Products: -
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3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+
7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-5beta-cholan-24-oic acid + NAD+
3-oxo-5beta-cholan-24-oic acid + NADH + H+
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Substrates: i.e. isolithocholic acid
Products: -
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3beta12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
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Substrates: i.e. isodeoxycholic acid
Products: -
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7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
additional information
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12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
Substrates: -
Products: -
?
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
Substrates: -
Products: -
?
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
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Substrates: -
Products: sole pruduct
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12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
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Substrates: -
Products: sole pruduct
?
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
Substrates: -
Products: sole pruduct
?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
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Substrates: -
Products: -
?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
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Substrates: -
Products: -
?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
Substrates: -
Products: -
?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
Substrates: -
Products: -
?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
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Substrates: -
Products: -
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3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+
7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
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Substrates: substrate docking of isoursodeoxycholic acid to the active site of a three-dimensional model of human class I gammagamma alcohol dehydrogenase reveals that the ADH gammagamma isozyme is the iso bile acid 3beta-hydroxysteroid dehydrogenase present in human liver cytosol, with NAD+ as a cofactor, but ethanol is the preferred substrate
Products: -
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3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+
7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
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Substrates: i.e. isoursodeoxycholic acid
Products: -
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7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
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Substrates: -
Products: -
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7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
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Substrates: -
Products: -
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7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
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Substrates: -
Products: -
?
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
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Substrates: -
Products: -
?
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
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Substrates: -
Products: -
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additional information
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Substrates: no activity with 3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid, 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid, 3alpha,7beta-dihydroxytaureate, 7,12-dioxo-5beta-cholanate, 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate and 3alpha-hydroxy-7,12-dioxo-5beta-cholanate
Products: -
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additional information
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Substrates: substrate specificity, bile acid screening, reaction product identification and quantification by gas chromotography/mass spectrometry, overview. No activity with 3alpha,7alpha,12alpha,27-tetrahydroxy-5beta-cholestane
Products: -
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additional information
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Substrates: the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value
Products: -
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additional information
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Substrates: the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value
Products: -
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12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+
7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
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Substrates: substrate docking of isoursodeoxycholic acid to the active site of a three-dimensional model of human class I gammagamma alcohol dehydrogenase reveals that the ADH gammagamma isozyme is the iso bile acid 3beta-hydroxysteroid dehydrogenase present in human liver cytosol, with NAD+ as a cofactor, but ethanol is the preferred substrate
Products: -
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
Substrates: -
Products: -
?
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
Substrates: -
Products: -
?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
Substrates: -
Products: -
?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
Substrates: -
Products: -
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
Substrates: -
Products: -
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
Substrates: -
Products: -
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Edenharder, R.; Pfutzner, A.; Hammann, R.
Characterization of NAD-dependent 3alpha- and 3beta-hydroxysteroid dehydrogenase and of NADP-dependent 7beta-hydroxysteroid dehydrogenase from Peptostreptococcus productus
Biochim. Biophys. Acta
1004
230-238
1989
Blautia producta, Blautia producta K2/25-1
brenda
Edenharder, R.; Pfuetzner, M.
Partial purification and characterization of an NAD-dependent 3beta-hydroxysteroid dehydrogenase from Clostridium innocuum
Appl. Environ. Microbiol.
55
1656-1659
1989
[Clostridium] innocuum, [Clostridium] innocuum D1144/3
brenda
Marschall, H.U.; Oppermann, U.C.; Svensson, S.; Nordling, E.; Persson, B.; Hoeoeg, J.O.; Joernvall, H.
Human liver class I alcohol dehydrogenase gammagamma isozyme: the sole cytosolic 3beta-hydroxysteroid dehydrogenase of iso bile acids
Hepatology
31
990-996
2000
Homo sapiens
brenda
Devlin, A.; Fischbach, M.
A biosynthetic pathway for a prominent class of microbiota-derived bile acids
Nat. Chem. Biol.
11
685-690
2015
Eggerthella lenta (C8WGQ3), Eggerthella lenta (C8WJW0), Eggerthella lenta ATCC 25559 (C8WGQ3), Eggerthella lenta ATCC 25559 (C8WJW0)
brenda