Any feedback?
Information on EC 1.1.1.399 - 2-oxoglutarate reductase
for references in articles please use BRENDA:EC1.1.1.399Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.1.1.399 2-oxoglutarate reductaseIUBMB Comments
The enzyme catalyses a reversible reaction. The enzyme from the bacterium Peptoniphilus asaccharolyticus is specific for (R)-2-hydroxyglutarate [1,2]. The SerA enzyme from the bacterium Escherichia coli can also accept (S)-2-hydroxyglutarate with a much higher Km, and also catalyses the activity of EC 1.1.1.95, phosphoglycerate dehydrogenase .
Specify your search results
Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
2-oxoglutarate reductase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alpha-ketoglutarate reductase
OGR
serA
serA
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(R)-2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH + H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
-
,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
(R)-2-hydroxyglutarate,NAD+ 2-oxidireductase
The enzyme catalyses a reversible reaction. The enzyme from the bacterium Peptoniphilus asaccharolyticus is specific for (R)-2-hydroxyglutarate [1,2]. The SerA enzyme from the bacterium Escherichia coli can also accept (S)-2-hydroxyglutarate with a much higher Km, and also catalyses the activity of EC 1.1.1.95, phosphoglycerate dehydrogenase [3].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-2-hydroxyglutarate + NAD+
2-oxoglutarate + NADH + H+
-
-
-
-
r
(R)-2-hydroxyglutarate + NAD+
2-oxoglutarate + NADH + H+
-
-
-
-
r
(R)-2-hydroxyglutarate + NAD+
2-oxoglutarate + NADH + H+
-
-
-
-
r
2-oxoglutarate + NADH + H+
(R)-2-hydroxyglutarate + NAD+
-
-
-
-
r
2-oxoglutarate + NADH + H+
(R)-2-hydroxyglutarate + NAD+
-
-
-
-
r
2-oxoglutarate + NADH + H+
(R)-2-hydroxyglutarate + NAD+
-
-
-
-
r
additional information
?
-
-
no activity with oxaloacetate, pyruvate, 2-oxobutyrate and glyoxylate
-
-
?
additional information
?
-
-
NADPH is oxidized in the presence of 2-oxoglutarate. The rate of this reaction is 3 to 4% of that observed with NADH
-
-
?
additional information
?
-
-
oxaloacetate, pyruvate, 2-oxobutyrate, 2-oxoisovalerate, and glyoxalate are not reduced by the enzyme
-
-
?
additional information
?
-
-
NADPH is oxidized in the presence of 2-oxoglutarate. The rate of this reaction is 3 to 4% of that observed with NADH
-
-
?
additional information
?
-
-
oxaloacetate, pyruvate, 2-oxobutyrate, 2-oxoisovalerate, and glyoxalate are not reduced by the enzyme
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-2-hydroxyglutarate + NAD+
2-oxoglutarate + NADH + H+
-
-
-
-
r
(R)-2-hydroxyglutarate + NAD+
2-oxoglutarate + NADH + H+
-
-
-
-
r
(R)-2-hydroxyglutarate + NAD+
2-oxoglutarate + NADH + H+
-
-
-
-
r
2-oxoglutarate + NADH + H+
(R)-2-hydroxyglutarate + NAD+
-
-
-
-
r
2-oxoglutarate + NADH + H+
(R)-2-hydroxyglutarate + NAD+
-
-
-
-
r
2-oxoglutarate + NADH + H+
(R)-2-hydroxyglutarate + NAD+
-
-
-
-
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
sodium acetate, acetyl phosphate, acetyl coenzyme A, glutamate, histidine, adenosine mono-, di-, and triphosphates, dithiothreitol, N-ethylmaleimide or iodoacetate at a concentration of 0.01-1 mM have no effect on the rate of the reaction
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). SERA_HUMAN
533
0
56651
Swiss-Prot
other Location (Reliability: 2)
SERA_MACFA
533
0
56518
Swiss-Prot
other Location (Reliability: 2)
SERA_MYCBO
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
528
0
54554
Swiss-Prot
-
SERA_MYCTO
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
528
0
54554
Swiss-Prot
-
SERA_MYCTU
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
528
0
54554
Swiss-Prot
-
SERA_PANTR
533
0
56663
Swiss-Prot
other Location (Reliability: 2)
SERA_PIG
533
0
56810
Swiss-Prot
other Location (Reliability: 2)
SERA_PONAB
533
0
56512
Swiss-Prot
other Location (Reliability: 2)
SERA_SCHPO
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
466
0
50883
Swiss-Prot
other Location (Reliability: 2)
SERA_SYNY3
Synechocystis sp. (strain PCC 6803 / Kazusa)
554
0
59222
Swiss-Prot
-
SERA_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
469
0
51193
Swiss-Prot
other Location (Reliability: 2)
SER33_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
469
0
51188
Swiss-Prot
other Location (Reliability: 2)
SERA_BOVIN
533
0
56452
Swiss-Prot
other Location (Reliability: 2)
SERA_DICDI
407
0
44749
Swiss-Prot
other Location (Reliability: 3)
SERA_ECOL6
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
410
0
44176
Swiss-Prot
-
SERA_HAEIN
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
410
0
44665
Swiss-Prot
-
HGDH_ACIFV
Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / CCUG 9996 / CIP 106432 / VR4)
331
0
36586
Swiss-Prot
-
X0QB61_9LACO
388
0
42030
TrEMBL
-
A0A3V8D846_SALET
410
0
43988
TrEMBL
-
A0A1I0D7B0_9FIRM
387
0
42066
TrEMBL
-
A0A701XR19_SALER
410
0
43987
TrEMBL
-
A0A6J8BD08_MYTCO
517
0
55236
TrEMBL
other Location (Reliability: 3)
A0A423KPL1_9PSED
409
0
44228
TrEMBL
-
A0A3Q9L3Q3_SALET
410
0
43988
TrEMBL
-
A0A0P9IPN0_9PSED
409
0
44313
TrEMBL
-
A0A2M7M5N7_9GAMM
380
0
41675
TrEMBL
-
A0A0R0E8B9_9GAMM
413
0
44347
TrEMBL
-
A0A198GCV0_9GAMM
411
0
44697
TrEMBL
-
C4FE30_9BIFI
399
0
43410
TrEMBL
-
J3AA24_ACTNH
Actinomyces naeslundii (strain ATCC 12104 / DSM 43013 / CCUG 2238 / JCM 8349 / NCTC 10301 / Howell 279)
396
0
42213
TrEMBL
-
Q0AA58_ALKEH
Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
389
0
41853
TrEMBL
-
A0A0R2CMC1_9LACO
382
0
42275
TrEMBL
-