Information on EC 1.1.1.77 - lactaldehyde reductase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.77
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RECOMMENDED NAME
GeneOntology No.
lactaldehyde reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)[or (S)]-propane-1,2-diol + NAD+ = (R)[or (S)]-lactaldehyde + NADH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ethylene glycol degradation
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L-lactaldehyde degradation (anaerobic)
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methylglyoxal degradation VI
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L-lactaldehyde degradation
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Glyoxylate and dicarboxylate metabolism
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Propanoate metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
(R)[or (S)]-propane-1,2-diol:NAD+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-15-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene rhaO
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Manually annotated by BRENDA team
Microcyclus eburneus
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-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-propane-1,2-diol + NAD+
(R)-lactaldehyde + NADH
show the reaction diagram
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
show the reaction diagram
ethanol + NAD+
acetaldehyde + NADH
show the reaction diagram
ethylene glycol + NAD+
glycolaldehyde + NAD+
show the reaction diagram
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-
-
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r
glycerol + NAD+
DL-glyceraldehyde + NADH
show the reaction diagram
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-
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r
L-1,2-propanediol + NAD+
L-lactaldehyde + NADH + H+
show the reaction diagram
propanol + NAD+
propionaldehyde + NADH
show the reaction diagram
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-
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r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
show the reaction diagram
L-1,2-propanediol + NAD+
L-lactaldehyde + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ascorbate
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0.025 mM FeCl3 and 0.035 mM ascorbate causes a 90% enzyme inactivation after 120 min = inactivation of metal-catalyzed oxidation; several amino-acids prevent the inactivation; under aerobic conditions 0.025 mM FeCl3 causes a 50% enzyme inactivation after 120 min
Fe2+
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under anaerobic conditions 0.01 mM Fe2+ and 0.1 mM H2O2 had the effect of 85% enzyme inactivation after 2 min, with addition of 0.0001 mM catalase the inactivation is only 25% after 2 min and with addition of 0.01 mM superoxide dismutase instead of catalse the inactivation is again 82% after 2 min
Fe3+
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0.025 mM FeCl3 and 0.035 mM ascorbate causes a 90% enzyme inactivation after 120 min = inactivation of metal-catalyzed oxidation; several amino-acids prevent the inactivation; under aerobic conditions 0.025 mM FeCl3 causes a 50% enzyme inactivation after 120 min
H2O2
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under anaerobic conditions 0.01 mM Fe2+ and 0.1 mM H2O2 had the effect of 85% enzyme inactivation after 2 min, with addition of 0.0001 mM catalase the inactivation is only 25% after 2 min and with addition of 0.01 mM superoxide dismutase instead of catalse the inactivation is again 82% after 2 min
iodoacetate
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L-1,2-propanediol
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above 25 mM: substrate inhibition
N-ethylmaleimide
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p-chloromercuribenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.065
acetaldehyde
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11
DL-1,2-Propanediol
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0.56
DL-lactaldehyde
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0.26
ethanol
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1.25
L-1,2-propanediol
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pH 9.5
0.035
L-lactaldehyde
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pH 7.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36.6
Microcyclus eburneus
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.5
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dehydrogenation of L-1,2-propanediol
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 10
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reduction of lactaldehyde, pH 4.5: 50% of reductase activity maximum, pH 10: 10% of reductase activity maximum
6 - 7.8
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reduction of DL-lactaldehyde, pH 6: 50% of reductase activity maximum, pH 7.8: 45% of reductase activity maximum
8 - 11
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dehydrogenation of 1,2-propanediol, pH 8: 10% of dehydrogenase activity maximum, pH 11: 65% of dehydrogenase activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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enzyme assay performed at this temperature
30
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enzyme assay performed at this temperature
43
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enzyme assay performed at this temperature
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 43
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Escherichia coli (strain K12);
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
Microcyclus eburneus
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4 * 38000, SDS-PAGE
39000
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2 * 39000, SDS-PAGE
76000
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gel permeation chromatography on a calibrated column of Ultrogel AcA 34 under denaturating conditions
155000
Microcyclus eburneus
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gel filtration on Sephadex G-200
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 39000, SDS-PAGE
tetramer
Microcyclus eburneus
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4 * 38000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crude extract, ammonium sulfate precipitation, heat treatment, ultrogel AcA 34 fractionation, DEAE-Sephadex column chromatography, second ultrogel AcA 34 fractionation
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crude extract, protamine sulfate, ammonium sulfate, DEAE-Sephadex-25, hydroxylapatite, blue dextran polyacrylamide
Microcyclus eburneus
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extraction, heat treatment, ammonium sulfate-fractionation, DEAE-cellulose and Alumina C
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene rhaO, encoded in the rhaKIPAO gene cluster, expression analysis, transcriptional regulation of the rhaKIPAOR gene cluster, overview
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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fermentation of L-rhamnose, L-fucose and D-fucose to a mixture of 1,2-propanediol, acetone, H2, CO2 and ethanol
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