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EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
d-gluconate dehydrogenase, gluconate oxidase, gluconic acid dehydrogenase, flavin adenine dinucleotide-containing ga 2-dehydrogenase,
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2-ketogluconate reductase
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D-gluconate 2-dehydrogenase
D-gluconate dehydrogenase
D-gluconate dehydrogenase, 2-keto-D-gluconate yielding
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dehydrogenase, gluconate 2-
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flavin adenine dinucleotide-containing GA 2-dehydrogenase
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gluconate oxidase
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gluconate-2-dehydrogenase
gluconic acid dehydrogenase
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gluconic dehydrogenase
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Cj0414/Cj0415
Q0PB96; Q0PB95
subunit Cj0414 and subunit Cj0415
Cj0414/Cj0415
Q0PB96; Q0PB95
subunit Cj0414 and subunit Cj0415
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D-gluconate 2-dehydrogenase
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D-gluconate 2-dehydrogenase
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D-gluconate dehydrogenase
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D-gluconate dehydrogenase
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D-gluconate dehydrogenase
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GA2DH
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GADH
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gluconate dehydrogenase
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gluconate dehydrogenase
Q0PB96; Q0PB95
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gluconate dehydrogenase
Q0PB96; Q0PB95
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gluconate-2-dehydrogenase
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gluconate-2-dehydrogenase
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D-gluconate + acceptor = 2-dehydro-D-gluconate + reduced acceptor
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oxidation
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oxidation
Q0PB96; Q0PB95
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reduction
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reduction
Q0PB96; Q0PB95
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-gluconate:acceptor 2-oxidoreductase
A flavoprotein (FAD).
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D-gluconate + 2,6-dichlorophenolindophenol
2-oxogluconate + ?
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
D-gluconate + acceptor
2-oxogluconate + reduced acceptor
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one of the key enzymes of gluconate catabolism
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D-gluconate + coenzyme Q1
2-oxogluconate + ?
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?
D-gluconate + FAD
2-dehydro-D-gluconate + FADH2
D-gluconate + ferricyanide
2-dehydro-D-gluconate + ferrocyanide
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?
D-gluconate + ferricyanide
2-oxogluconate + ferrocyanide
D-gluconate + oxidized 2,6-dichlorophenolindophenol
2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol
D-gluconate + phenazine methosulfate
2-oxogluconate + ?
additional information
?
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no activity with menadione
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?
D-gluconate + 2,6-dichlorophenolindophenol
2-oxogluconate + ?
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?
D-gluconate + 2,6-dichlorophenolindophenol
2-oxogluconate + ?
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?
D-gluconate + 2,6-dichlorophenolindophenol
2-oxogluconate + ?
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?
D-gluconate + 2,6-dichlorophenolindophenol
2-oxogluconate + ?
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D-gluconate + 2,6-dichlorophenolindophenol
2-oxogluconate + ?
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?
D-gluconate + 2,6-dichlorophenolindophenol
2-oxogluconate + ?
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D-gluconate + 2,6-dichlorophenolindophenol
2-oxogluconate + ?
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?
D-gluconate + 2,6-dichlorophenolindophenol
2-oxogluconate + ?
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?
D-gluconate + 2,6-dichlorophenolindophenol
2-oxogluconate + ?
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?
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
Q0PB96; Q0PB95
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D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
Q0PB96; Q0PB95
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?
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
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?
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
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?
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
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?
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
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?
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
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?
D-gluconate + FAD
2-dehydro-D-gluconate + FADH2
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D-gluconate + FAD
2-dehydro-D-gluconate + FADH2
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D-gluconate + FAD
2-dehydro-D-gluconate + FADH2
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?
D-gluconate + ferricyanide
2-oxogluconate + ferrocyanide
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?
D-gluconate + ferricyanide
2-oxogluconate + ferrocyanide
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?
D-gluconate + ferricyanide
2-oxogluconate + ferrocyanide
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?
D-gluconate + ferricyanide
2-oxogluconate + ferrocyanide
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?
D-gluconate + oxidized 2,6-dichlorophenolindophenol
2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol
Q0PB96; Q0PB95
activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS
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?
D-gluconate + oxidized 2,6-dichlorophenolindophenol
2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol
Q0PB96; Q0PB95
activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS
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D-gluconate + phenazine methosulfate
2-oxogluconate + ?
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D-gluconate + phenazine methosulfate
2-oxogluconate + ?
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?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
D-gluconate + acceptor
2-oxogluconate + reduced acceptor
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one of the key enzymes of gluconate catabolism
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D-gluconate + FAD
2-dehydro-D-gluconate + FADH2
D-gluconate + oxidized 2,6-dichlorophenolindophenol
2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
Q0PB96; Q0PB95
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?
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
Q0PB96; Q0PB95
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?
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
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?
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
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?
D-gluconate + acceptor
2-dehydro-D-gluconate + reduced acceptor
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?
D-gluconate + FAD
2-dehydro-D-gluconate + FADH2
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?
D-gluconate + FAD
2-dehydro-D-gluconate + FADH2
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D-gluconate + FAD
2-dehydro-D-gluconate + FADH2
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?
D-gluconate + oxidized 2,6-dichlorophenolindophenol
2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol
Q0PB96; Q0PB95
activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS
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?
D-gluconate + oxidized 2,6-dichlorophenolindophenol
2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol
Q0PB96; Q0PB95
activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS
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?
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8alpha-(N1-histidyl)-FAD
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prosthetic group
additional information
Q0PB96; Q0PB95
a putative NAD, FAD binding site is located within the first 48 amino acids of Cj0415
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cytochrome c1
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dehydrogenase protein is tightly bound to cytochrome c1 component
cytochrome c1
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contains a cytochrome c1, c-554(551), which might by a diheme cytochrome
FAD
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flavin
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contains covalently bound flavin
flavin
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contains covalently bound flavin
heme
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heme
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contains 16.8 nmol of heme per mg of protein
heme
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enzyme contains 2 mol of heme per mol of enzyme
heme
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contains 14.6 nmol of heme per mg of protein
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2-oxogluconate
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competitive
o-benzoquinone
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0.5 mM, complete inhibition
Quinacrine hydrochloride
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1 mM, 10% loss of activity
oxalate
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oxamate
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oxamate
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6.7 mM, 97% inhibition, non-competitive
oxamate
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1 mM, 91% loss of activity
pyruvate
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Quinine hydrochloride
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1.0 mM, 55% loss of activity
Quinine hydrochloride
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1 mM, 42% loss of activity
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cardiolipin
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stimulates in presence of Triton X-100
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0.21
2,6-dichlorophenolindophenol
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0.15
phenazine methosulfate
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0.3
D-gluconate
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pH 6
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29 - 123
2-oxo-D-gluconate
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2-oxo-D-gluconate
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pH 5.0
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2-oxo-D-gluconate
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pH 6.0
2.8
oxalate
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0.07
oxamate
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pH 5.0
4.6
pyruvate
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pH 5.0
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0.0013
Q0PB96; Q0PB95
cells grown at 37°C, Cj0415 mutant
0.0019
Q0PB96; Q0PB95
cells grown at 42°C, Cj0414 mutant
0.0026
Q0PB96; Q0PB95
cells grown at 37°C, Cj0414 mutant
0.0045
Q0PB96; Q0PB95
cells grown at 42°C, Cj0415 mutant
0.0141
Q0PB96; Q0PB95
cells grown at 37°C, wild type
0.0333
Q0PB96; Q0PB95
cells grown at 42°C, wild type
additional information
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5.5
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reaction with coenzyme Q1
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direct electron transfer-type bioelectrocatalytic oxidation of D-gluconate is observed at pH 4.0
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reaction with ferricyanide
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activity assay
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reaction with ferricyanide
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reaction with ferricyanide
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Q0PB96; Q0PB95
activity assay
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phenazine methosulfate
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4 - 6
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pH 4.0: about 40% of maximal activity, pH 6.0: about 60% of maximal activity
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25
Q0PB96; Q0PB95
activity assay
40
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5.5
Q0PB96; Q0PB95
predicted, subunit Cj0414
8.8
Q0PB96; Q0PB95
predicted, subunit Cj0415
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subunit Cj0414 and subunit Cj0415
Q0PB96; Q0PB95
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brenda
subunit Cj0414 and subunit Cj0415
Q0PB96; Q0PB95
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brenda
NBRC 3271
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brenda
NBRC 3271
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brenda
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brenda
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brenda
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brenda
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brenda
ATCC 29267
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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Uniprot
brenda
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brenda
IFO 3271
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brenda
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Q0PB96; Q0PB95
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brenda
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brenda
Q0PB96; Q0PB95
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brenda
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brenda
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brenda
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brenda
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brenda
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bound to
brenda
membrane bound
brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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reconstitutive experiments on the enzyme with membrane fraction from which the enzyme has been solubilized and removed
brenda
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brenda
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124000 - 131000
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sucrose density gradient centrifugation
132000 - 138000
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non-denaturing PAGE
15500
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1 * 15500 + 1 * 52500 + 1 * 68000, SDS-PAGE
21000
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1 * 21000 + 1 * 45000 + 1 * 64000, SDS-PAGE
21700
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1 * 21700 + 1 * 47500 + 1 * 66500, SDS-PAGE
24000
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1 * 24000, subunit of unknown function, + 1 * 47000, cytochrome c subunit, + 1 * 68000, dehydrogenase subunit, calculation from nucleotide sequence, SDS-PAGE
26900
Q0PB96; Q0PB95
predicted, subunit Cj0414
45000
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1 * 21000 + 1 * 45000 + 1 * 64000, SDS-PAGE
47000
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1 * 24000, subunit of unknown function, + 1 * 47000, cytochrome c subunit, + 1 * 68000, dehydrogenase subunit, calculation from nucleotide sequence, SDS-PAGE
47500
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1 * 21700 + 1 * 47500 + 1 * 66500, SDS-PAGE
50000
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1 * 22000, subunit of unclear function, + 1 * 50000, cytochrome c1, + 1 * 66000, flavoprotein, SDS-PAGE
52000
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1 * 22000 + 1 * 52000 + 1 * 66000, SDS-PAGE
52500
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1 * 15500 + 1 * 52500 + 1 * 68000, SDS-PAGE
63700
Q0PB96; Q0PB95
predicted, subunit Cj0415
64000
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1 * 21000 + 1 * 45000 + 1 * 64000, SDS-PAGE
66500
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1 * 21700 + 1 * 47500 + 1 * 66500, SDS-PAGE
22000
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1 * 22000 + 1 * 52000 + 1 * 66000, SDS-PAGE
22000
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1 * 22000, subunit of unclear function, + 1 * 50000, cytochrome c1, + 1 * 66000, flavoprotein, SDS-PAGE
66000
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1 * 22000 + 1 * 52000 + 1 * 66000, SDS-PAGE
1.1.99.3 gluconate 2-dehydrogenase (acceptor)
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