Information on EC 1.1.99.41 - 3-hydroxy-1,2-didehydro-2,3-dihydrotabersonine reductase

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The expected taxonomic range for this enzyme is: Catharanthus roseus

EC NUMBER
COMMENTARY hide
1.1.99.41
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RECOMMENDED NAME
GeneOntology No.
3-hydroxy-1,2-didehydro-2,3-dihydrotabersonine reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(3R)-3-hydroxy-16-methoxy-2,3-dihydrotabersonine + acceptor = (3R)-3-hydroxy-16-methoxy-1,2-didehydro-2,3-dihydrotabersonine + reduced acceptor
show the reaction diagram
(1)
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(3R)-3-hydroxy-2,3-dihydrotabersonine + acceptor = (3R)-3-hydroxy-1,2-didehydro-2,3-dihydrotabersonine + reduced acceptor
show the reaction diagram
(1)
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
vindoline and vinblastine biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(3R)-3-hydroxy-16-methoxy-2,3-dihydrotabersonine:acceptor oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
16-hydroxytabersonine + NADPH + H+
?
show the reaction diagram
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-
?
16-methoxytabersonine + NADPH + H+
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
show the reaction diagram
2,3-dihydrotabersonine + NADPH + H+
?
show the reaction diagram
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-
-
?
tabersonine + NADPH + H+
2,3-dihydro-3-hydroxytabersonine + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
16-methoxytabersonine + NADPH + H+
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
show the reaction diagram
A0A161CAI1
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-
-
?
tabersonine + NADPH + H+
2,3-dihydro-3-hydroxytabersonine + NADP+
show the reaction diagram
A0A161CAI1
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?
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21-(DE3) cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H189Y
the mutation diminishes the activity of the enzyme by 95%