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2,6-dihydroxy-4-[[(2E,6E,10E)-12-hydroxy-2,6,10-trimethyldodeca-2,6,10-trien-1-yl]oxy]benzoic acid
-
-
2-fluoro-4-[[(2E,6E)-8-hydroxy-2,6-dimethylocta-2,6-dien-1-yl]oxy]benzoic acid
-
-
2-heptyl-4-hydroxyquinoline N-oxide
-
i.e. HQNO, binds stoichiometrically to the enzyme and prevents formation of the ubisemiquinone at the QH-site, but does not displace the ubiquinone-8 bound at the QH-site, enzyme binding kineticss, overview
2-Heptyl-4-hydroxyquinoline-N-oxide
-
2-hydroxy-4-[[(2E,6E)-8-hydroxy-2,6-dimethylocta-2,6-dien-1-yl]oxy]-6-methylbenzoic acid
-
-
2-hydroxy-4-[[(2E,6E)-8-hydroxy-2,6-dimethylocta-2,6-dien-1-yl]oxy]benzoic acid
-
-
2-hydroxybenzhydroxamic acid
-
competitive inhibitor towards ubiquinol
4-[(14-bromotetradecyl)oxy]-2-hydroxybenzaldehyde
-
-
4-[[(2E,6E)-8-hydroxy-2,6-dimethylocta-2,6-dien-1-yl]oxy]-2-methylbenzoic acid
-
-
5-chloro-3-[(2E)-3,7-dimethylocta-2,6-dienyl]-2,4-dihydroxy-6-methylbenzaldehyde
an ascofuranone derivative
5-chloro-3-[(2E,6E)-8-hydroxy-3,7-dimethylnona-2,6-dienyl]-2,4-dihydroxy-6-methylbenzaldehyde
an ascofuranone derivative
antimycin A
56% residual activity at 0.005 mg/ml; 56% residual activity at 0.005 mg/ml
aurachin C 1-10
15% residual activity at 0.005 mg/ml; 15% residual activity at 0.005 mg/ml
aurachin C1-10
-
prevents formation of the ubisemiquinone at the QH-site, but appears to compete for quinol binding at the QL-site, enzyme binding kineticss, overview
aurachin D
16% residual activity at 0.005 mg/ml; 16% residual activity at 0.005 mg/ml
ferulenol
competitive inhibitor, binding is completely reversible
-
Gramicidin S
67% residual activity at 0.005 mg/ml; 67% residual activity at 0.005 mg/ml
LL-Z1272gamma
69% residual activity at 0.005 mg/ml; 69% residual activity at 0.005 mg/ml
methyl 2-hydroxy-4-[[(2E,6E)-8-hydroxy-2,6-dimethylocta-2,6-dien-1-yl]oxy]benzoate
-
-
methyl 4-[(14-bromotetradecyl)oxy]-2-hydroxybenzoate
-
-
Piericidin A
14% residual activity at 0.005 mg/ml; 14% residual activity at 0.005 mg/ml
salicylic hydroxamic acid
-
ubiquinol-1
-
substrate inhibition
ubiquinol-2
-
substrate inhibition
ubiquinone-1
-
product inhibition, an excess amount of ubiquinol-2 is unable to suppress product inhibition with ubiquinone-1 therefore, the inhibition mode may not be competitive
ubiquinone-2
-
product inhibition
[14-(4-carboxy-3-hydroxy-5-methylphenoxy)tetradecyl](triphenyl)phosphanium
-
-
[14-(4-formyl-3-hydroxyphenoxy)tetradecyl](triphenyl)phosphanium
-
-
[14-[3-fluoro-4-(methoxycarbonyl)phenoxy]tetradecyl](triphenyl)phosphanium
-
-
[14-[3-hydroxy-4-(methoxycarbonyl)phenoxy]tetradecyl](triphenyl)phosphanium
-
-
[14-[4-(ethoxycarbonyl)-3-hydroxy-5-methylphenoxy]tetradecyl](triphenyl)phosphanium
-
-
[14-[4-hydroxy-3-(methoxycarbonyl)phenoxy]tetradecyl](triphenyl)phosphanium
-
-
ascochlorin
-
-
ascofuranone
-
almost complete inhibition at 10 nM
ascofuranone
-
almost complete inhibition at 10 nM
ascofuranone
an antibiotic
ascofuranone
-
specifically inhibits the quinol oxidase activity of TAO
ascofuranone
quasi-irreversible inhibitor
colletochlorin B
-
mixed-type inhibitor
-
cyanide
-
cyanide
quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration; quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration
n-octyl gallate
-
the enzyme is fully sensitive to 0.001 mM octyl gallate
n-octyl gallate
-
the enzyme is fully sensitive to 0.001 mM octyl gallate
n-propyl gallate
-
-
octyl gallate
-
-
octylgallate
weak inhibition
octylgallate
weak inhibition
Salicylhydroxamic acid
-
-
Salicylhydroxamic acid
weak inhibition
Salicylhydroxamic acid
-
-
Salicylhydroxamic acid
weak inhibition
ubiquinol
-
AOX is inactivated by its product ubiquinol during catalysis, this inhibition is prevented in the presence of pyruvate. The inhibition can be reversed by a reductive process, achieved by high levels of reduction of the ubiquinone-pool or by dithiothreitol
ubiquinol
-
AOX is inactivated by its product ubiquinol during catalysis, this inhibition is prevented in the presence of pyruvate. The inhibition can be reversed by a reductive process, achieved by high levels of reduction of the ubiquinone-pool or by dithiothreitol
additional information
-
the enzyme is cyanide- and antimycin-resistant
-
additional information
-
not inhibited by cyanide
-
additional information
-
presence of high affinity inhibitors, 2-heptyl-4-hydroxyquinoline N-oxide and aurachin C110, does not displace ubiquinone-8 from the QH site
-
additional information
-
not inhibited by ascofuranone; not inhibited by ascofuranone
-
additional information
not inhibited by ascofuranone; not inhibited by ascofuranone
-
additional information
-
CIO activity is much more resistant to cyanide, compared with Escherichia coli cytochrome bd, but sensitive to azide
-
additional information
-
not inhibited by myxothiazol
-
additional information
-
insensitive to cyanide
-
additional information
-
the respiratory activity exhibited by mitochondria containing the wild type AOX is partially resistant to antimycin A (about 18% of the NADH-dependent rate)
-
additional information
-
the enzyme is cyanide- and antimycin-resistant
-
additional information
inhibitor binding induces the ligation of a histidine residue in the active site, inhibitor binding site and structures, overview
-
additional information
-
inhibitor binding induces the ligation of a histidine residue in the active site, inhibitor binding site and structures, overview
-
additional information
-
insensitive to cyanide
-
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Bacterial Infections
Transient transcriptional regulation of the CYS-C1 gene and cyanide accumulation upon pathogen infection in the plant immune response.
Carcinoma
The respiratory behavior of Lewis carcinoma cells--existence of a cyanide-resistant respiration.
Cytochrome-c Oxidase Deficiency
Diiron centre mutations in Ciona intestinalis alternative oxidase abolish enzymatic activity and prevent rescue of cytochrome oxidase deficiency in flies.
Cytochrome-c Oxidase Deficiency
Expression of alternative oxidase in Drosophila ameliorates diverse phenotypes due to cytochrome oxidase deficiency.
Cytochrome-c Oxidase Deficiency
Expression of the alternative oxidase complements cytochrome c oxidase deficiency in human cells.
Cytochrome-c Oxidase Deficiency
The alternative oxidase AOX does not rescue the phenotype of tko25t mutant flies.
Cytochrome-c Oxidase Deficiency
The alternative oxidase, a tool for compensating cytochrome c oxidase deficiency in human cells
Cytochrome-c Oxidase Deficiency
The alternative oxidase, a tool for compensating cytochrome c oxidase deficiency in human cells.
Cytochrome-c Oxidase Deficiency
The male sterile G cytoplasm of wild beet displays modified mitochondrial respiratory complexes.
Dehydration
Effects of water stress on development, operation and gene expression of cyanide-resistant respiratory pathway in wheat.
Dehydration
Effects of water stress on respiration in soybean leaves.
Dehydration
Implications of terminal oxidase function in regulation of salicylic acid on soybean seedling photosynthetic performance under water stress.
Dehydration
The response difference of mitochondria in recalcitrant Antiaris toxicaria axes and orthodox Zea mays embryos to dehydration injury.
Dehydration
Water stress enhances expression of genes encoding plastid terminal oxidase and key components of chlororespiration and alternative respiration in soybean seedlings.
Heart Diseases
Sea squirt alternative oxidase bypasses fatal mitochondrial heart disease.
Infections
Alternative oxidase mediates pathogen resistance in Paracoccidioides brasiliensis infection.
Infections
Alternative oxidase reduces the sensitivity of Mycosphaerella graminicola to QOI fungicides.
Infections
Disruption of the alternative oxidase gene in Magnaporthe grisea and its impact on host infection.
Infections
Enhanced expression and activation of the alternative oxidase during infection of Arabidopsis with Pseudomonas syringae pv tomato.
Infections
N-n-alkyl-3,4-dihydroxybenzamides as inhibitors of the trypanosome alternative oxidase: activity in vitro and in vivo.
Infections
The Effects of Salicylic Acid and Tobacco Mosaic Virus Infection on the Alternative Oxidase of Tobacco.
Infections
The influence of RNA-dependent RNA polymerase 1 on potato virus Y infection and on other antiviral response genes.
Infections
Toward More Drug Like Inhibitors of Trypanosome Alternative Oxidase.
Infertility, Female
Phenotypic rescue of a Drosophila model of mitochondrial ANT1 disease.
Infertility, Male
Partial male sterility in transgenic tobacco carrying an antisense gene for alternative oxidase under the control of a tapetum-specific promoter.
Malaria
Alternative oxidase inhibitors potentiate the activity of atovaquone against Plasmodium falciparum.
Mitochondrial Diseases
Alternative NAD(P)H dehydrogenase and alternative oxidase: Proposed physiological roles in animals.
Mitochondrial Diseases
Engineering the alternative oxidase gene to better understand and counteract mitochondrial defects: state of the art and perspectives.
nadh:ubiquinone reductase (h+-translocating) deficiency
Alternative oxidase rescues mitochondria-mediated dopaminergic cell loss in Drosophila.
Paracoccidioidomycosis
Alternative oxidase mediates pathogen resistance in Paracoccidioides brasiliensis infection.
Phytoplasma Disease
Heterologous expression of an alternative oxidase from Moniliophthora perniciosa in Saccharomyces cerevisiae: antioxidant function and in vivo platform for the study of new drugs against witches' broom disease.
Phytoplasma Disease
Synthesis and testing of novel alternative oxidase (AOX) inhibitors with antifungal activity against Moniliophthora perniciosa (Stahel), the causal agent of witches' broom disease of cocoa, and other phytopathogens.
Starvation
An Adaptation to Low Copper in Candida albicans Involving SOD Enzymes and the Alternative Oxidase.
Starvation
Nitric oxide induces the alternative oxidase pathway in Arabidopsis seedlings deprived of inorganic phosphate.
Starvation
Regulation of alternative oxidase 1 in Chlamydomonas reinhardtii during sulfur starvation.
Starvation
Whole-plant respiration and its temperature sensitivity during progressive carbon starvation
Starvation
Whole-plant respiration and its temperature sensitivity during progressive carbon starvation.
Trypanosomiasis
Crystallization and preliminary crystallographic analysis of cyanide-insensitive alternative oxidase from Trypanosoma brucei brucei.
Trypanosomiasis
Probing the ubiquinol-binding site of recombinant Sauromatum guttatum alternative oxidase expressed in E. coli membranes through site-directed mutagenesis.
Trypanosomiasis
Purification and kinetic characterization of recombinant alternative oxidase from Trypanosoma brucei brucei.
Trypanosomiasis
The alternative oxidases: simple oxidoreductase proteins with complex functions.
Trypanosomiasis, African
African trypanosomiasis: Synthesis & SAR enabling novel drug discovery of ubiquinol mimics for trypanosome alternative oxidase.
Trypanosomiasis, African
Functional expression of the ascofuranone-sensitive Trypanosoma brucei brucei alternative oxidase in the cytoplasmic membrane of Escherichia coli.
Trypanosomiasis, African
Overproduction of highly active trypanosome alternative oxidase in Escherichia coli heme-deficient mutant.
Trypanosomiasis, African
Strain-specific difference in amino acid sequences of trypanosome alternative oxidase.
Trypanosomiasis, African
Trypanosome alternative oxidase as a target of chemotherapy.
Trypanosomiasis, African
Trypanosome alternative oxidase: from molecule to function.
Trypanosomiasis, African
[Mitochondria as targets of chemotherapy].
Tuberculosis
The carboxy-terminal insert in the Q-loop is needed for functionality of Escherichia coli cytochrome bd-I.
ubiquinol oxidase (non-electrogenic) deficiency
Effects of AOX1a deficiency on plant growth, gene expression of respiratory components, and metabolic profile under low-nitrogen stress in Arabidopsis thaliana plants.
ubiquinol oxidase (non-electrogenic) deficiency
Importance of the alternative oxidase (AOX) pathway in regulating cellular redox and ROS homeostasis to optimize photosynthesis during restriction of the cytochrome oxidase pathway in Arabidopsis thaliana.
Virus Diseases
Ethylene is involved in leafy mustard systemic resistance to Turnip mosaic virus infection through the mitochondrial alternative oxidase pathway
Virus Diseases
Inhibition of Satellite RNA Associated Cucumber Mosaic Virus Infection by Essential Oil of Micromeria croatica (Pers.) Schott.
Virus Diseases
The Effects of Salicylic Acid and Tobacco Mosaic Virus Infection on the Alternative Oxidase of Tobacco.
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0.0000031
2,6-dihydroxy-4-[[(2E,6E,10E)-12-hydroxy-2,6,10-trimethyldodeca-2,6,10-trien-1-yl]oxy]benzoic acid
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.0011
2-fluoro-4-[[(2E,6E)-8-hydroxy-2,6-dimethylocta-2,6-dien-1-yl]oxy]benzoic acid
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.013
2-Heptyl-4-hydroxyquinoline-N-oxide
Gluconobacter oxydans
at 25°C in 50 mM potassium phosphate (pH 6.5)
0.0024
2-hydroxy-4-[[(2E,6E)-8-hydroxy-2,6-dimethylocta-2,6-dien-1-yl]oxy]-6-methylbenzoic acid
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.0021
2-hydroxy-4-[[(2E,6E)-8-hydroxy-2,6-dimethylocta-2,6-dien-1-yl]oxy]benzoic acid
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.0000176
4-[(14-bromotetradecyl)oxy]-2-hydroxybenzaldehyde
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.00092
4-[[(2E,6E)-8-hydroxy-2,6-dimethylocta-2,6-dien-1-yl]oxy]-2-methylbenzoic acid
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.00000048
5-chloro-3-[(2E,6E)-8-hydroxy-3,7-dimethylnona-2,6-dienyl]-2,4-dihydroxy-6-methylbenzaldehyde
Trypanosoma brucei
pH and temperature not specified in the publication
0.017
antimycin A
Gluconobacter oxydans
at 25°C in 50 mM potassium phosphate (pH 6.5)
0.0000037 - 0.000316
ascochlorin
-
0.000000288 - 0.000129
ascofuranone
0.00004
aurachin C 1-10
Gluconobacter oxydans
at 25°C in 50 mM potassium phosphate (pH 6.5)
0.82
azide
Gluconobacter oxydans
-
pH 6.5, 25°C
0.0000025
colletochlorin
Trypanosoma brucei brucei
pH 7.5, 25°C
-
0.0000075 - 0.00047
colletochlorin B
-
0.00000154 - 0.00009
colletochlorin D
-
0.00000142
ferulenol
Trypanosoma brucei brucei
pH 7.4, 25°C
-
0.04
Gramicidin S
Gluconobacter oxydans
at 25°C in 50 mM potassium phosphate (pH 6.5)
0.013
LL-Z1272gamma
Gluconobacter oxydans
at 25°C in 50 mM potassium phosphate (pH 6.5)
0.00169
methyl 2-hydroxy-4-[[(2E,6E)-8-hydroxy-2,6-dimethylocta-2,6-dien-1-yl]oxy]benzoate
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.0023
methyl 4-[(14-bromotetradecyl)oxy]-2-hydroxybenzoate
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.00007 - 0.0001
octyl gallate
0.0000023 - 0.000862
octyl-gallate
-
0.0007
Piericidin A
Gluconobacter oxydans
at 25°C in 50 mM potassium phosphate (pH 6.5)
0.00036 - 0.00044
propyl gallate
0.00593 - 0.194
Salicylhydroxamic acid
0.0000037 - 0.047
salicylic hydroxamic acid
0.0000042
[14-(4-carboxy-3-hydroxy-5-methylphenoxy)tetradecyl](triphenyl)phosphanium
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.00022
[14-(4-formyl-3-hydroxyphenoxy)tetradecyl](triphenyl)phosphanium
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.00074
[14-[3-fluoro-4-(methoxycarbonyl)phenoxy]tetradecyl](triphenyl)phosphanium
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.000015
[14-[3-hydroxy-4-(methoxycarbonyl)phenoxy]tetradecyl](triphenyl)phosphanium
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.00025
[14-[4-(ethoxycarbonyl)-3-hydroxy-5-methylphenoxy]tetradecyl](triphenyl)phosphanium
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.000012
[14-[4-hydroxy-3-(methoxycarbonyl)phenoxy]tetradecyl](triphenyl)phosphanium
Trypanosoma brucei brucei
37°C, pH not specified in the publication
-
0.0000037
ascochlorin
Trypanosoma brucei brucei
pH 7.5, 25°C
-
0.0000074
ascochlorin
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, wild-type enzyme
-
0.0000147
ascochlorin
Arabidopsis thaliana
pH 7.5, 25°C
-
0.000167
ascochlorin
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96A
-
0.000203
ascochlorin
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100A
-
0.000215
ascochlorin
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme L212A
-
0.000221
ascochlorin
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100E
-
0.000316
ascochlorin
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96K
-
0.000000288
ascofuranone
Trypanosoma brucei brucei
pH 7.4, 25°C
0.0000013
ascofuranone
Trypanosoma brucei brucei
pH 7.5, 25°C
0.000002
ascofuranone
Trypanosoma brucei brucei
pH and temperature not specified in the publication
0.000007
ascofuranone
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, wild-type enzyme
0.000038
ascofuranone
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme L212A
0.000051
ascofuranone
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100A
0.000058
ascofuranone
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96A
0.000089
ascofuranone
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100E
0.000129
ascofuranone
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96K
0.0000075
colletochlorin B
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, wild-type enzyme
-
0.000036
colletochlorin B
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme L212A
-
0.0000593
colletochlorin B
Moniliophthora perniciosa
-
pH 7.4, temperature not specified in the publication
-
0.000066
colletochlorin B
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96K
-
0.000137
colletochlorin B
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100A
-
0.000239
colletochlorin B
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100E
-
0.00047
colletochlorin B
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96A
-
0.00000154
colletochlorin D
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96A
-
0.0000038
colletochlorin D
Trypanosoma brucei brucei
pH 7.5, 25°C
-
0.000033
colletochlorin D
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, wild-type enzyme
-
0.000051
colletochlorin D
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96K
-
0.000061
colletochlorin D
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme L212A
-
0.000081
colletochlorin D
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100A
-
0.00009
colletochlorin D
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100E
-
0.008
cyanide
Gluconobacter oxydans
quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration with the IC50 of 0.008 mM (relative amplitude, 21%) and 0.013 mM (79%), at 25°C in 50 mM potassium phosphate (pH 6.5)
13
cyanide
Gluconobacter oxydans
quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration with the IC50 of 0.008 mM (relative amplitude, 21%) and 13 mM (79%), at 25°C in 50 mM potassium phosphate (pH 6.5)
0.00007
octyl gallate
Sauromatum venosum
mutant Y299F, pH and temperature not specified in the publication
0.00008
octyl gallate
Sauromatum venosum
mutants C172A and T179A, pH and temperature not specified in the publication
0.00009
octyl gallate
Sauromatum venosum
wild-type enzyme, pH and temperature not specified in the publication
0.0001
octyl gallate
Sauromatum venosum
mutant Y253F, pH and temperature not specified in the publication
0.0000023
octyl-gallate
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96K
-
0.0000052
octyl-gallate
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100E
-
0.000046
octyl-gallate
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96A
-
0.00023
octyl-gallate
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, wild-type enzyme
-
0.000308
octyl-gallate
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100A
-
0.000862
octyl-gallate
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme L212A
-
0.00036
propyl gallate
Arabidopsis thaliana
-
mutant enzyme F215L, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C
0.00039
propyl gallate
Arabidopsis thaliana
-
mutant enzyme M219V, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C
0.00039
propyl gallate
Arabidopsis thaliana
-
wild type enzyme, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C
0.00042
propyl gallate
Arabidopsis thaliana
-
mutant enzyme M219I, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C
0.00044
propyl gallate
Arabidopsis thaliana
-
mutant enzyme G303E, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C
0.00593
Salicylhydroxamic acid
Trypanosoma brucei brucei
pH and temperature not specified in the publication
0.042
Salicylhydroxamic acid
Arabidopsis thaliana
-
wild type enzyme, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C
0.057
Salicylhydroxamic acid
Arabidopsis thaliana
-
mutant enzyme M219I, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C
0.069
Salicylhydroxamic acid
Arabidopsis thaliana
-
mutant enzyme F215L, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C
0.073
Salicylhydroxamic acid
Arabidopsis thaliana
-
mutant enzyme M219V, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C
0.194
Salicylhydroxamic acid
Arabidopsis thaliana
-
mutant enzyme G303E, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C
0.0000037
salicylic hydroxamic acid
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100E
0.0000044
salicylic hydroxamic acid
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme D100A
0.0000044
salicylic hydroxamic acid
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96K
0.0000061
salicylic hydroxamic acid
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, wild-type enzyme
0.000022
salicylic hydroxamic acid
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme R96A
0.047
salicylic hydroxamic acid
Trypanosoma brucei brucei
pH 7.4, temperature not specified in the publication, mutant enzyme L212A
0.012
sialic acid
Sauromatum venosum
mutant T179A, pH and temperature not specified in the publication
0.016
sialic acid
Sauromatum venosum
wild-type enzyme, pH and temperature not specified in the publication
0.018
sialic acid
Sauromatum venosum
mutant Y299F, pH and temperature not specified in the publication
0.019
sialic acid
Sauromatum venosum
mutant C172A, pH and temperature not specified in the publication
0.02
sialic acid
Sauromatum venosum
mutant Y253F, pH and temperature not specified in the publication
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drug target
-
the development of AOX-targeting antifungal agents against Moniliophthora perniciosa is an important outcome for the chocolate industry
drug target
validated as a drug target against trypanosomes
evolution
the cytochrome ba3 oxidase belongs to the family B of the heme-copper containing terminal oxidases, heme-copper oxidases use either c-type cytochromes or quinols as electron donors
evolution
-
the enzyme is a member of the subfamily of cytochrome bd present in bacterial respiratory chain, phylogenetic analysis
evolution
the enzyme is found in mitochondria of all higher plants studied to date
evolution
-
the enzyme is a member of the subfamily of cytochrome bd present in bacterial respiratory chain, phylogenetic analysis
-
malfunction
-
transgenic plant cells lacking mitochondrial alternative oxidase have increased susceptibility to mitochondria-dependent and -independent pathways of programmed cell death and show higher sensitivity to treatment with hydrogen peroxide, salicylic acid and cantharidin as compared to the wild type enzyme
malfunction
Glycine max antisense lines for AOX2b result in a decrease in photosynthesis rates, altered fertility, and slower vegetative growth
malfunction
-
inhibition of enzyme (AOX) activity by salicylhydroxamic acid signficantly alters the capacity of the fungus to grow and sporulate
malfunction
over 7000 genes, encoding proteins involved in antioxidant and other functions, are significantly altered in AOX1a knockout plants
malfunction
overexpression of PpAOX disturbes redox homeostasis in chloroplasts
malfunction
the deletion mutant strain is more sensitive than wild-type cells to high light under mixotrophic and photoautotrophic conditions
metabolism
-
the alternative oxidase actively competes with the cytochrome pathway for reducing equivalents and contributes up to 24% to the overall respiratory activity
metabolism
-
alternative oxidase is a key enzyme for cyanide-resistant respiration
metabolism
AOX isoforms from Arabidopsis are differentially fine-regulated by tricarboxylic acid cycle metabolites
metabolism
AOX isoforms from Arabidopsis are differentially fine-regulated by tricarboxylic acid cycle metabolites. Expression of isoenzyme AOX1D is increased in aox1a knockout mutants from Arabidopsis (especially after restriction of the cytochrome c pathway) but cannot compensate for the lack of AOX1A, suggesting a difference in the regulation of these isoforms
metabolism
-
expression of alternative oxidase (AOX) is able to promote cell migratory behavior in two different models: in Drosophila, AOX expression corrects thoracic closure defects produced by impaired signaling at several steps in the JNK pathway, while AOX-expressing immortalized mouse embryonic fibroblasts but not primary mouse embryonic fibroblasts show enhanced migration, which is abolished by JNK inhibitor V. AOX was unable to correct cell migration defects resulting from downregulation of the main JNK substrate, the c-Jun subunit of AP-1, or by manipulation of other factors, such as the AP-1 downstream target puc or the pnr transcription factor
metabolism
-
the AOX pathway could influence chloroplast energy metabolism and is essential for the maintenance of photosynthetic carbon assimilation in pepper leaves under normal conditions. Drought induces upregulation of the AOX pathway, which plays an important role in protection against drought-induced photoinhibition. The AOX pathway could optimize carbon assimilation and PSII function in plants experiencing drought, which could help avoid overreduction of PSII. Inhibition of AOX pathway could be compensated by increasing the thermal energy dissipation and cyclic electron flow around PSI (CEF-PSI) under drought stress, and the compensation of CEF-PSI is especially significant
physiological function
-
AppBC alleviates the accumulation of electrons in the quinone pool during respiratory stress via electroneutral ubiquinol oxidation
physiological function
AOX is a diiron carboxylate protein that catalyzes the four-electron reduction of dioxygen to water by ubiquinol. AOX plays a critical role in the survival of the parasite in its bloodstream form
physiological function
-
cytochrome bo3 oxidase catalyzes the 2-electron oxidation of ubiquinol-8 and the 4-electron reduction of dioxygen to water
physiological function
the alternative oxidase (AOX) is a non-protonmotive ubiquinol oxidase
physiological function
-
ubiquinol-10 molecules are reoxidized by cytochrome bo3 and CIO, terminal oxidases of the respiratory chain. In the Gluconobacter oxydans respiratory chain, CIO may have a physiological role in compensation for lower activity of cytochrome bo3 under low growth pH to maintain rapid substrate oxidation
physiological function
-
enzyme signaling regulates the greening process
physiological function
-
plastid signals enhance plant cold stress tolerance mainly through the induction of the gene AOX1a
physiological function
-
the alternative oxidase pathway plays an important role in the sodium nitroprusside-elevated resistance of Medicago to salt stress
physiological function
-
the enzyme is central for metabolic heat-production
physiological function
the enzyme is required for the N gene-mediated resistance to Tobacco mosaic virus
physiological function
-
the expression of AOX1a in Saccharomyces cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage
physiological function
AOX1a is involved in adaptation to As(V)-induced oxidative stress
physiological function
critical enzyme for the respiration of bloodstream forms of trypanosomes
physiological function
the alternative oxidase (AOX) pathway is the most effective pathway in maintaining cellular redox and energy balance, especially under stress conditions, including light stress
physiological function
the alternative oxidase (AOX) pathway is the most effective pathway in maintaining cellular redox and energy balance, especially under stress conditions, including light stress
physiological function
the alternative oxidase (AOX) pathway is the most effective pathway in maintaining cellular redox and energy balance, especially under stress conditions, including light stress. AOX dissipates excess reductants produced in the chloroplasts, and thereby prevents photooxidation
physiological function
-
the alternative oxidase (AOX) pathway is the most effective pathway in maintaining cellular redox and energy balance, especially under stress conditions, including light stress. The alternative oxidase in the regulation of cellular homeostasis during development of photosynthetic function in greening leaves
physiological function
-
the AOX pathway plays an important role in photoprotection in Malus hupehensis leaves under drought stress
physiological function
the Chlamydomonas AOX proteins (CrAOX1 and CrAOX2) can participate in acclimation of Chlamydomonas reinhardtii cells to excess absorbed light energy
physiological function
-
the enzyme (AOX) is determinant for growth and sporulation. It participates in life cycle control in Blastocladiella emersonii
physiological function
-
the enzyme (MpAOX) is crucial for survival the fungal pathogen Moniliophthora perniciosa (causal agent of the witches' broom disease of cocoa)
physiological function
-
the enzyme is important for plant growth and performance under a variety of stress or adverse growth conditions
physiological function
the enzyme is important for plant growth and performance under a variety of stress or adverse growth conditions. During severe or prolonged mild drought stress in, the amount of AOX protein is important to maintain photosynthetic performance. Enzyme form AOX1a is under strong transcriptional suppression
physiological function
the enzyme is required for optimal photosynthesis and growth, and gametophyte development
physiological function
the enzyme participates in plant salt tolerance in Physcomitrella patens and there is a functional link between mitochondria and chloroplast under challenge conditions
physiological function
the enzyme plays a central role in metabolism through facilitating the turnover of the TCA cycle whilst reducing ROS production
physiological function
-
the enzyme plays an essential role in ethylene-induced drought tolerance and also played important roles in mediating autophagy generation via balancing ROS level
physiological function
the enzyme reduces stress in Aliivibrio fischeri cells exposed to nitric oxide
physiological function
-
ubiquinol-10 molecules are reoxidized by cytochrome bo3 and CIO, terminal oxidases of the respiratory chain. In the Gluconobacter oxydans respiratory chain, CIO may have a physiological role in compensation for lower activity of cytochrome bo3 under low growth pH to maintain rapid substrate oxidation
-
physiological function
-
AppBC alleviates the accumulation of electrons in the quinone pool during respiratory stress via electroneutral ubiquinol oxidation
-
additional information
-
analysis of a one-site Q-site model and a two-site Q-site model, overview
additional information
putative ubiquinol binding cavities, overview. The nonheme diiron carboxylate active site is buried within a four-helix bundle. The active site is ligated solely by four glutamate residues in its oxidized inhibitor-free state. Highly conserved Tyr220 iswithin 4 A of the active site and is critical for catalytic activity. The enzyme is a homodimer with two hydrophobic cavities per monomer. Both cavities bind ubiquinol and along with Tyr220 are required for the catalytic cycle for O2 reduction, but also inhibitors bind to one cavity and Tyr220, respectively. A second cavity interacts with the inhibitor-binding cavity at the diiron center. The active site, which is located in a hydrophobic environment deep inside the enzyme molecule, is composed of the diiron center and four glutamate (E123, E162, E213, and E266) and two histidine residues (H165 and H269), all of which are completely conserved
additional information
-
putative ubiquinol binding cavities, overview. The nonheme diiron carboxylate active site is buried within a four-helix bundle. The active site is ligated solely by four glutamate residues in its oxidized inhibitor-free state. Highly conserved Tyr220 iswithin 4 A of the active site and is critical for catalytic activity. The enzyme is a homodimer with two hydrophobic cavities per monomer. Both cavities bind ubiquinol and along with Tyr220 are required for the catalytic cycle for O2 reduction, but also inhibitors bind to one cavity and Tyr220, respectively. A second cavity interacts with the inhibitor-binding cavity at the diiron center. The active site, which is located in a hydrophobic environment deep inside the enzyme molecule, is composed of the diiron center and four glutamate (E123, E162, E213, and E266) and two histidine residues (H165 and H269), all of which are completely conserved
additional information
the enzyme can be reduced by ubiquinol, but it alone can also be reduced by decylubiquinol and N,N,N',N'-tetramethyl-4-phenylenediamine/ascorbate in the presence of cyanide
additional information
-
the enzyme can be reduced by ubiquinol, but it alone can also be reduced by decylubiquinol and N,N,N',N'-tetramethyl-4-phenylenediamine/ascorbate in the presence of cyanide
additional information
-
the purified CIO shows an extraordinary high ubiquinol-1 oxidase activity. The enzyme shows a modified ping-pong bi-bi mechanism
additional information
AOX1c is not affected by As(V) treatments (0.1, 0.2 or 0.3 mM)
additional information
AOX1c is not affected by As(V) treatments (0.1, 0.2 or 0.3 mM)
additional information
AOX1c is not affected by As(V) treatments (0.1, 0.2 or 0.3 mM)
additional information
AOX1c is not affected by As(V) treatments (0.1, 0.2 or 0.3 mM)
additional information
AOX1c is not affected by As(V) treatments (0.1, 0.2 or 0.3 mM)
additional information
-
developmental arrest in Drosophila melanogaster caused by mitochondrial DNA replication defects cannot be rescued by xenotopic expression of the alternative oxidase (AOX) from Ciona intestinalis. AOX does not rescue developmental lethality, nor alter the biochemical and molecular parameters associated with Twinkle and pol gamma mutations or knockdown. This restricts the potential therapeutic use of AOX to specific types of mitochondrial dysfunction
additional information
-
the purified CIO shows an extraordinary high ubiquinol-1 oxidase activity. The enzyme shows a modified ping-pong bi-bi mechanism
-
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C127S
-
the substitution prevents oxidative inactivation of alternative oxidase and renders the protein insensitive to pyruvate activation, the mutated protein is instead activated specifically by succinate
C78A
-
the mutant shows significant (418%) stimulation by 5 mM succinate and little response to 5 mM pyruvate
C78D
-
the mutant is significantly stimulated (28%) by 5 mM succinate, pyruvate has no significant effect on the mutant enzyme activity
C78E
-
the mutant is significantly stimulated (37%) by 5 mM succinate
C78K
-
the mutant is insensitive to pyruvate or succinate but more active than the wild type without pyruvate
C78R
-
the mutant is insensitive to pyruvate or succinate but more active than the wild type without pyruvate
C78S
-
the mutant is significantly (489%) stimulated by 5 mM succinate
F215L
-
the mutant exhibits 1.6fold resistance to salicylhydroxamic acid compared to the wild type enzyme
G303E
-
the mutant exhibits 4.6fold resistance to salicylhydroxamic acid compared to the wild type enzyme
M219I
-
the mutant exhibits 1.4fold resistance to salicylhydroxamic acid compared to the wild type enzyme
M219V
-
the mutant exhibits 1.7fold resistance to salicylhydroxamic acid compared to the wild type enzyme
D188A
-
site-directed mutagenesis, the mutant shows altered sensitivity to inhibitor aurachin C1-10 compared to the wild-type enzyme, The mutant oxidase is not able to support aerobic growth when expressed in a strain of Escherichia coli without a genomically encoded respiratory oxidase
D188N
-
site-directed mutagenesis, the mutant shows altered sensitivity to inhibitor aurachin C1-10 compared to the wild-type enzyme
D75E
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows similar activity compared to the wild-type enzyme
D75H
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows highly reduced enzyme activity compared to the wild-type enzyme
D75N
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows highly reduced enzyme activity compared to the wild-type enzyme
D75R
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows highly reduced enzyme activity compared to the wild-type enzyme
H98N
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows highly reduced enzyme activity compared to the wild-type enzyme
H98T
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows highly reduced enzyme activity compared to the wild-type enzyme
Q101N
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows highly reduced enzyme activity compared to the wild-type enzyme
R257Q
-
site-directed mutagenesis, the mutant shows altered sensitivity to inhibitor aurachin C1-10 compared to the wild-type enzyme. The mutant oxidase is not able to support aerobic growth when expressed in a strain of Escherichia coli without a genomically encoded respiratory oxidase
R71D
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows highly reduced enzyme activity compared to the wild-type enzyme
R71D/D75R
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows highly reduced enzyme activity compared to the wild-type enzyme
R71K
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows highly reduced enzyme activity compared to the wild-type enzyme
R71Q
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows highly reduced enzyme activity compared to the wild-type enzyme
WI36A
-
site-directed mutagenesis, the mutant shows altered sensitivity to inhibitor aurachin C1-10 compared to the wild-type enzyme
C99S
-
the substitution prevents oxidative inactivation of alternative oxidase and renders the protein insensitive to pyruvate activation, the mutated protein is instead activated specifically by succinate
C172A
site-directed mutagenesis, the mutant shows reduced activity and oxygen affinity compared to the wild-type enzyme
E217A
-
the mutation results in the loss of AOX activity
E270N
-
the mutation results in the loss of AOX activity
T179A
site-directed mutagenesis, the mutant shows reduced activity and oxygen affinity compared to the wild-type enzyme
W206F
site-directed mutagenesis, inactive mutant
W206Y
site-directed mutagenesis, inactive mutant
Y275F
-
the mutant exhibits barely detectable mitochondrial antimycin-resistant respiratory activity
Y299F
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H261A
-
the mutant shows 5% activity compared to the wild type enzyme
N247Q
-
the mutant shows 96% activity compared to the wild type enzyme
Q242N
-
the mutant shows 6% activity compared to the wild type enzyme
R262K
-
the mutant shows 6% activity compared to the wild type enzyme
S256T
-
the mutant shows 7% activity compared to the wild type enzyme
Y253A
-
the mutant shows 28% activity compared to the wild type enzyme
Y253F
-
the mutant shows 61% activity compared to the wild type enzyme
H261A
-
the mutant shows 5% activity compared to the wild type enzyme
-
N247Q
-
the mutant shows 96% activity compared to the wild type enzyme
-
Q242N
-
the mutant shows 6% activity compared to the wild type enzyme
-
Y253A
-
the mutant shows 28% activity compared to the wild type enzyme
-
Y253F
-
the mutant shows 61% activity compared to the wild type enzyme
-
A216L
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity
A216N
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity
E213A
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity
E215A
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity
L122A
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity
L122N
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity
R118A
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity
R118Q
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity
T219V
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity
Y220F
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity
Y246A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
D100A
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 8.8fold lower than the kcat/Km-value of wild-type enzyme
D100E
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 5.7fold lower than the kcat/Km-value of wild-type enzyme
E215A
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 34fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid
E215D
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 528fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid
E215N
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 567fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid
L122A
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 34.3fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid
L212A
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 31.2fold lower than the kcat/Km-value of wild-type enzyme
R118A
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 22.5fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid
R118K
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 19.6fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid
R96A
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 7.9fold lower than the kcat/Km-value of wild-type enzyme
R96K
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 7.8fold lower than the kcat/Km-value of wild-type enzyme
T219A
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 36.4fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid
T219S
kcat/Km-value of the mutant enzyme for ubiquinol-1 is 89.1fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid
H98S
-
site-directed mutagenesis at the QH-site of cytochrome bo3, the mutant shows highly reduced enzyme activity compared to the wild-type enzyme
H98S
-
site-directed mutagenesis, the mutant shows altered sensitivity to inhibitor aurachin C1-100 compared to the wild-type enzyme
Y253F
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y253F
-
the mutant exhibits a mitochondrial antimycin-resistant respiratory activity that is comparable with that of the wild type
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Kido, Y.; Shiba, T.; Inaoka, D.K.; Sakamoto, K.; Nara, T.; Aoki, T.; Honma, T.; Tanaka, A.; Inoue, M.; Matsuoka, S.; Moore, A.; Harada, S.; Kita, K.
Crystallization and preliminary crystallographic analysis of cyanide-insensitive alternative oxidase from Trypanosoma brucei brucei
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Isolation of mutants of the Arabidopsis thaliana alternative oxidase (ubiquinol:oxygen oxidoreductase) resistant to salicylhydroxamic acid
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Arum italicum, Glycine max
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Robson, C.A.; Vanlerberghe, G.C.
Transgenic plant cells lacking mitochondrial alternative oxidase have increased susceptibility to mitochondria-dependent and -independent pathways of programmed cell death
Plant Physiol.
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1908-1920
2002
Nicotiana tabacum
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Williams, B.A.; Elliot, C.; Burri, L.; Kido, Y.; Kita, K.; Moore, A.L.; Keeling, P.J.
A broad distribution of the alternative oxidase in microsporidian parasites
PLoS Pathog.
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2010
Antonospora locustae, Trachipleistophora hominis
brenda
Crichton, P.; Albury, M.; Affourtit, C.; Moore, A.
Mutagenesis of the Sauromatum guttatum alternative oxidase reveals features important for oxygen binding and catalysis
Biochim. Biophys. Acta
1797
732-737
2010
Sauromatum venosum (P22185), Sauromatum venosum
brenda
Miura, H.; Mogi, T.; Ano, Y.; Migita, C.T.; Matsutani, M.; Yakushi, T.; Kita, K.; Matsushita, K.
Cyanide-insensitive quinol oxidase (CIO) from Gluconobacter oxydans is a unique terminal oxidase subfamily of cytochrome bd
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Gluconobacter oxydans, Gluconobacter oxydans NBRC 3172
brenda
Gao, Y.; Meyer, B.; Sokolova, L.; Zwicker, K.; Karas, M.; Brutschy, B.; Peng, G.; Michel, H.
Heme-copper terminal oxidase using both cytochrome c and ubiquinol as electron donors
Proc. Natl. Acad. Sci. USA
109
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2012
Aquifex aeolicus (G5DGC8), Aquifex aeolicus
brenda
Shiba, T.; Kido, Y.; Sakamoto, K.; Inaoka, D.K.; Tsuge, C.; Tatsumi, R.; Takahashi, G.; Balogun, E.O.; Nara, T.; Aoki, T.; Honma, T.; Tanaka, A.; Inoue, M.; Matsuoka, S.; Saimoto, H.; Moore, A.L.; Harada, S.; Kita, K.
Structure of the trypanosome cyanide-insensitive alternative oxidase
Proc. Natl. Acad. Sci. USA
110
4580-4585
2013
Trypanosoma brucei (Q26710), Trypanosoma brucei
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Vishwakarma, A.; Dalal, A.; Tetali, S.D.; Kirti, P.B.; Padmasree, K.
Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis
FEBS Open Bio
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135-146
2016
Arabidopsis thaliana
brenda
Zhang, B.; Zhu, D.; Wang, G.; Peng, G.
Characterization of the AOX gene and cyanide-resistant respiration in Pyropia haitanensis (Rhodophyta)
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Neoporphyra haitanensis
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brenda
Jian, W.; Zhang, D.W.; Zhu, F.; Wang, S.X.; Pu, X.J.; Deng, X.G.; Luo, S.S.; Lin, H.H.
Alternative oxidase pathway is involved in the exogenous SNP-elevated tolerance of Medicago truncatula to salt stress
J. Plant Physiol.
193
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2016
Medicago truncatula
brenda
Zhang, D.W.; Yuan, S.; Xu, F.; Zhu, F.; Yuan, M.; Ye, H.X.; Guo, H.Q.; Lv, X.; Yin, Y.; Lin, H.H.
Light intensity affects chlorophyll synthesis during greening process by metabolite signal from mitochondrial alternative oxidase in Arabidopsis
Plant Cell Environ.
39
12-25
2016
Arabidopsis thaliana
brenda
Tang, H.; Zhang, D.; Yuan, S.; Zhu, F.; Xu, F.; Fu, F.; Wang, S.; Lin, H.
Plastid signals induce alternative oxidase expression to enhance the cold stress tolerance in Arabidopsis thaliana
Plant Growth Regul.
74
275-283
2014
Arabidopsis thaliana
brenda
Zhu, F.; Deng, X.G.; Xu, F.; Jian, W.; Peng, X.J.; Zhu, T.; Xi, D.H.; Lin, H.H.
Mitochondrial alternative oxidase is involved in both compatible and incompatible host-virus combinations in Nicotiana benthamiana
Plant Sci.
239
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2015
Nicotiana benthamiana (U5XGW7), Nicotiana benthamiana
brenda
Sayed, M.A.; Umekawa, Y.; Ito, K.
Metabolic interplay between cytosolic phosphoenolpyruvate carboxylase and mitochondrial alternative oxidase in thermogenic skunk cabbage, Symplocarpus renifolius
Plant Signal. Behav.
11
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2016
Symplocarpus renifolius
brenda
Yoshioka, I.; Kobayashi, K.; Kirimura, K.
Overexpression of the gene encoding alternative oxidase for enhanced glucose consumption in oxalic acid producing Aspergillus niger expressing oxaloacetate hydrolase gene
J. Biosci. Bioeng.
120
172-176
2019
Aspergillus niger
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Meco-Navas, A.; Ebiloma, G.; Martinguez, A.; Martinez-Benayas, I.; Cueto-Diaz, E.; Alhejely, A.; Balogun, E.; Saito, M.; Matsui, M.; Arai, N.; Shiba, T.; Harada, S.; De Koning, H.; Dardonville, C.
SAR of 4-alkoxybenzoic acid inhibitors of the Trypanosome alternative oxidase
ACS Med. Chem. Lett.
9
923-928
2018
Trypanosoma brucei brucei (Q26710)
brenda
Sun, S.; Xu, X.; Liu, Q.; Gao, H.
Mitochondrial alternative oxidase pathway protects photosynthetic apparatus against photodamage by alleviating photoinhibition in Malus hupehensis leaves under drought stress
Acta Hortic.
1261
115-121
2019
Malus hupehensis
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brenda
Shiba, T.; Inaoka, D.; Takahashi, G.; Tsuge, C.; Kido, Y.; Young, L.; Ueda, S.; Balogun, E.; Nara, T.; Honma, T.; Tanaka, A.; Inoue, M.; Saimoto, H.; Harada, S.; Moore, A.; Kita, K.
Insights into the ubiquinol/dioxygen binding and proton relay pathways of the alternative oxidase
Biochim. Biophys. Acta Bioenerg.
1860
375-382
2019
Trypanosoma brucei brucei (Q26710)
brenda
Young, L.; Rosell-Hidalgo, A.; Inaoka, D.K.; Xu, F.; Albury, M.; May, B.; Kita, K.; Moore, A.L.
Kinetic and structural characterisation of the ubiquinol-binding site and oxygen reduction by the trypanosomal alternative oxidase
Biochim. Biophys. Acta Bioenerg.
1861
148247
2020
Trypanosoma brucei brucei (Q26710)
brenda
Barsottini, M.; Copsey, A.; Young, L.; Baroni, R.; Cordeiro, A.; Pereira, G.; Moore, A.
Biochemical characterization and inhibition of the alternative oxidase enzyme from the fungal phytopathogen Moniliophthora perniciosa
Commun. Biol.
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263
2020
Moniliophthora perniciosa
brenda
Hewitt, S.; Dhingra, A.
Beyond Ethylene New insights regarding the role of alternative oxidase in the respiratory climacteric
Front. Plant Sci.
11
543958
2020
Triticum urartu (M7Z212), Arabidopsis thaliana (Q39219), Glycine max (Q41266)
brenda
Xu, F.
Comparison of the kinetic parameters of alternative oxidases from Trypanosoma brucei and Arabidopsis thaliana-A tale of two cavities
Front. Plant Sci.
12
744218
2021
Trypanosoma brucei brucei (Q26710), Arabidopsis thaliana (Q39219)
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Luevano-Martinez, L.A.; Caldeira da Silva, C.C.; Nicastro, G.G.; Schumacher, R.I.; Kowaltowski, A.J.; Gomes, S.L.
Mitochondrial alternative oxidase is determinant for growth and sporulation in the early diverging fungus Blastocladiella emersonii
Fungal Biol.
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2019
Blastocladiella emersonii
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Dunn, A.
Alternative oxidase activity reduces stress in Vibrio fischeri cells exposed to nitric oxide
J. Bacteriol.
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2018
Aliivibrio fischeri (Q5E7C3), Aliivibrio fischeri
brenda
Kaye, Y.; Huang, W.; Clowez, S.; Saroussi, S.; Idoine, A.; Sanz-Luque, E.; Grossman, A.R.
The mitochondrial alternative oxidase from Chlamydomonas reinhardtii enables survival in high light
J. Biol. Chem.
294
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Chlamydomonas reinhardtii (O65000), Chlamydomonas reinhardtii (Q9FE26), Chlamydomonas reinhardtii
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Liao, Y.; Cui, R.; Yuan, T.; Xie, Y.; Gao, Y.
Cysteine and methionine contribute differentially to regulate alternative oxidase in leaves of poplar (Populus deltoides x Populus euramericana 'Nanlin 895') seedlings exposed to different salinity
J. Plant Physiol.
240
153017
2019
Populus deltoides x Populus x canadensis
brenda
Andjelkovic, A.; Mordas, A.; Bruinsma, L.; Ketola, A.; Cannino, G.; Giordano, L.; Dhandapani, P.; Szibor, M.; Dufour, E.; Jacobs, H.
Expression of the alternative oxidase influences jun N-terminal kinase signaling and cell migration
Mol. Cell. Biol.
38
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2018
Ciona intestinalis
brenda
Hu, W.; Yan, X.; He, Y.; Ye, X.
Role of alternative oxidase pathway in protection against drought-induced photoinhibition in pepper leaves
Photosynthetica
56
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2018
Capsicum annuum
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brenda
Garmash, E.V.
Role of mitochondrial alternative oxidase in the regulation of cellular homeostasis during development of photosynthetic function in greening leaves
Plant Biol.
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2021
Triticum aestivum
brenda
Zhu, T.; Zou, L.; Li, Y.; Yao, X.; Xu, F.; Deng, X.; Zhang, D.; Lin, H.
Mitochondrial alternative oxidase-dependent autophagy involved in ethylene-mediated drought tolerance in Solanum lycopersicum
Plant Biotechnol. J.
16
2063-2076
2018
Solanum lycopersicum
brenda
Demircan, N.; Cucun, G.; Uzilday, B.
Mitochondrial alternative oxidase (AOX1a) is required for the mitigation of arsenic-induced oxidative stress in Arabidopsis thaliana
Plant Biotechnol. Rep.
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235-245
2020
Arabidopsis thaliana (A0A1P8AP13), Arabidopsis thaliana (O22048), Arabidopsis thaliana (O22049), Arabidopsis thaliana (O23913), Arabidopsis thaliana (Q39219)
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Wu, G.; Li, S.; Li, X.; Liu, Y.; Zhao, S.; Liu, B.; Zhou, H.; Lin, H.
A functional alternative oxidase modulates plant salt tolerance in Physcomitrella patens
Plant Cell Physiol.
60
1829-1841
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Physcomitrium patens (A9T8C5), Physcomitrium patens
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Selinski, J.; Hartmann, A.; Deckers-Hebestreit, G.; Day, D.; Whelan, J.; Scheibe, R.
Alternative oxidase isoforms are differentially activated by tricarboxylic acid cycle intermediates
Plant Physiol.
176
1423-1432
2018
Arabidopsis thaliana (A0A1P8AP13), Arabidopsis thaliana (O22048), Arabidopsis thaliana (O22049), Arabidopsis thaliana (O23913), Arabidopsis thaliana (Q39219)
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Rodrigues, A.P.C.; Camargo, A.F.; Andjelkovic, A.; Jacobs, H.T.; Oliveira, M.T.
Developmental arrest in Drosophila melanogaster caused by mitochondrial DNA replication defects cannot be rescued by the alternative oxidase
Sci. Rep.
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10882
2018
Ciona intestinalis
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Selinski, J.; Scheibe, R.; Day, D.A.; Whelan, J.
Alternative oxidase is positive for plant performance
Trends Plant Sci.
23
588-597
2018
Neurospora crassa, Nicotiana tabacum, Macroptilium atropurpureum, Arabidopsis thaliana (Q39219), Arabidopsis thaliana, Glycine max (Q41266), Glycine max (Q7XZQ1)
brenda