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2,3-dihydroxybenzoate + O2
?
2,4-dihydroxybenzoate + O2
?
2,5-dihydroxybenzoate + O2
?
2,6-dihydroxybenzoate + O2
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
3,4-dihydroxybenzoate + O2
?
3,4-dihydroxybenzoate + O2
beta-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxyhydrocinnamic acid + O2
?
3,4-dihydroxymandelic acid + O2
?
3,4-dihydroxyphenylacetate + O2
?
3,4-dihydroxyphenylalanine + O2
?
Nocardia erythropolis
-
at 10.7% the rate of protocatechuic acid oxidation
-
-
?
3,4-dihydroxyphenylpropionic acid + O2
?
-
-
-
-
?
3,5-dihydroxybenzoate + O2
?
3-(3,4-dihydroxyphenyl)propionate + O2
?
-
protocatechuate 3,4-dioxygenase II
-
-
?
3-methylcatechol + O2
2-methylmuconate
4-cresol + O2
?
-
-
-
-
?
4-hydroxybenzoic acid + O2
?
4-methylcatechol + O2
3-methyl-cis,cis-muconate
4-methylcatechol + O2
?
-
-
-
-
?
4-sulfocatechol + O2
3-sulfomuconate
5-fluoro-protocatechuic acid + O2
5-fluoro-3-carboxy-cis,cis-muconate
-
at 2.1% the rate of protocatechuic acid oxidation
-
?
6-chloro-protocatechuate + O2
6-chloro-3-carboxy-cis,cis-muconate
-
at 4.3% the rate of protocatechuic acid oxidation
-
?
catechin + O2
?
-
-
-
-
?
catechol + O2
cis,cis-muconate
protocatechuate + O2
3-carboxy-cis,cis-muconate
protocatechuate + O2
beta-carboxy-cis-cis-muconate
protocatechuic acid + O2
3-carboxy-cis,cis-muconic acid
-
-
-
-
?
trans-3,4-dihydroxycinnamate + O2
?
additional information
?
-
2,3-dihydroxybenzoate + O2
?
23.6% activity compared to 3,4-dihydroxybenzoate
-
-
?
2,3-dihydroxybenzoate + O2
?
23.6% of the activity with protocatechuate for the free enzyme, 149.3% for the calcium alginate-immobilized enzyme, and 119.8% for the glyoxyl agarose-immobilized enzyme
-
-
?
2,4-dihydroxybenzoate + O2
?
43.5% activity compared to 3,4-dihydroxybenzoate
-
-
?
2,4-dihydroxybenzoate + O2
?
43.5% of the activity with protocatechuate for the free enzyme, 96.5% for the calcium alginate-immobilized enzyme, and 65.6% for the glyoxyl agarose-immobilized enzyme
-
-
?
2,5-dihydroxybenzoate + O2
?
33.46% activity compared to 3,4-dihydroxybenzoate
-
-
?
2,5-dihydroxybenzoate + O2
?
33.5% of the activity with protocatechuate for the free enzyme, 158.6% for the calcium alginate-immobilized enzyme, and 72.1% for the glyoxyl agarose-immobilized enzyme
-
-
?
2,6-dihydroxybenzoate + O2
?
30.5% of the activity with protocatechuate for the free enzyme, 90.4% for the calcium alginate-immobilized enzyme, and 36.2% for the glyoxyl agarose-immobilized enzyme
-
-
?
2,6-dihydroxybenzoate + O2
?
30.51% activity compared to 3,4-dihydroxybenzoate
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
absolute requirement for vicinal hydroxyl groups in the 3- and 4-position
-
ir
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 2-chloro-protocatechuate
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 5-bromo-protocatechuate
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 5-chloro-protocatechuate
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
methylene blue cannot replace O2 as electron acceptor
-
ir
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
Nocardia erythropolis
-
enzyme is active on a wide range of o-dihydroxyphenyl compounds
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with: 3,4-dihydroxyphenylacetic acid
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 3,4-dihydroxymandelic acid
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 4'-methylcatechol
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with: 3,4-dihydroxyphenylacetic acid
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 3,4-dihydroxymandelic acid
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 4'-methylcatechol
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
no other substrate found
-
ir
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
no other substrate found
-
ir
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
i.e. protocatechuate
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
specific for protocatechuate
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
best substrate
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
100% activity
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
100% activity
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
best substrate
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
no other substrate found
-
?
3,4-dihydroxybenzoate + O2
?
-
spectroscopic and electronic structure study of the enzyme-substrate complex
-
-
?
3,4-dihydroxybenzoate + O2
?
-
spectroscopic and electronic structure study of the enzyme-substrate complex
-
-
?
3,4-dihydroxyhydrocinnamic acid + O2
?
29.5% of the activity with protocatechuate for the free enzyme, 95.0% for the calcium alginate-immobilized enzyme, and 41.1% for the glyoxyl agarose-immobilized enzyme
-
-
?
3,4-dihydroxyhydrocinnamic acid + O2
?
29.54% ctivity compared to 3,4-dihydroxybenzoate
-
-
?
3,4-dihydroxyhydrocinnamic acid + O2
?
29.54% ctivity compared to 3,4-dihydroxybenzoate
-
-
?
3,4-dihydroxyhydrocinnamic acid + O2
?
29.5% of the activity with protocatechuate for the free enzyme, 95.0% for the calcium alginate-immobilized enzyme, and 41.1% for the glyoxyl agarose-immobilized enzyme
-
-
?
3,4-dihydroxymandelic acid + O2
?
-
protocatechuate 3,4-dioxygenase II
-
-
?
3,4-dihydroxymandelic acid + O2
?
-
protocatechuate 3,4-dioxygenase II
-
-
?
3,4-dihydroxymandelic acid + O2
?
Nocardia erythropolis
-
at 5.4% the rate of protocatechuic acid oxidation
-
-
?
3,4-dihydroxyphenylacetate + O2
?
-
-
-
-
?
3,4-dihydroxyphenylacetate + O2
?
-
W153V protocatechuate 3,4-dioxygenase I mutant enzyme: 13% activity compared to protocatechuate, wild-type protocatechuate 3,4-dioxygenase I: 2% activity compared to protocatechuate
-
-
?
3,4-dihydroxyphenylacetate + O2
?
-
-
-
-
?
3,5-dihydroxybenzoate + O2
?
44.3% of the activity with protocatechuate for the free enzyme, 113.3% for the calcium alginate-immobilized enzyme, and 42.9% for the glyoxyl agarose-immobilized enzyme
-
-
?
3,5-dihydroxybenzoate + O2
?
44.33% activity compared to 3,4-dihydroxybenzoate
-
-
?
3-methylcatechol + O2
2-methylmuconate
-
-
-
?
3-methylcatechol + O2
2-methylmuconate
-
-
-
?
3-methylcatechol + O2
2-methylmuconate
-
at 5% the rate of protocatechuic acid oxidation
-
?
3-methylcatechol + O2
2-methylmuconate
-
-
-
?
3-methylcatechol + O2
2-methylmuconate
-
-
-
?
3-methylcatechol + O2
2-methylmuconate
Nocardia erythropolis
-
at 14.4% the rate of protocatechuic acid oxidation
-
?
3-methylcatechol + O2
2-methylmuconate
-
at 0.4% the rate of protocatechuic acid oxidation
-
?
4-hydroxybenzoic acid + O2
?
-
-
-
?
4-hydroxybenzoic acid + O2
?
-
-
-
?
4-methylcatechol + O2
3-methyl-cis,cis-muconate
-
at very low rates
-
?
4-methylcatechol + O2
3-methyl-cis,cis-muconate
-
at very low rates
-
?
4-methylcatechol + O2
3-methyl-cis,cis-muconate
-
at very low rates
-
?
4-methylcatechol + O2
3-methyl-cis,cis-muconate
-
at very low rates
-
?
4-methylcatechol + O2
3-methyl-cis,cis-muconate
Nocardia erythropolis
-
at 3.1% the rate of protocatechuic acid oxidation
-
?
4-sulfocatechol + O2
3-sulfomuconate
-
catalyzed by protocatechuate 3,4-dioxygenase type II only
-
?
4-sulfocatechol + O2
3-sulfomuconate
-
catalyzed by protocatechuate 3,4-dioxygenase type II only
-
?
4-sulfocatechol + O2
3-sulfomuconate
-
catalyzed by protocatechuate 3,4-dioxygenase type II only
-
?
caffeic acid + O2
?
-
-
-
-
?
caffeic acid + O2
?
-
-
-
-
?
caffeic acid + O2
?
34.2% of the activity with protocatechuate for the free enzyme, 150% for the calcium alginate-immobilized enzyme, and 97% for the glyoxyl agarose-immobilized enzyme
-
-
?
caffeic acid + O2
?
34.21% activity compared to 3,4-dihydroxybenzoate
-
-
?
caffeic acid + O2
?
34.21% activity compared to 3,4-dihydroxybenzoate
-
-
?
caffeic acid + O2
?
34.2% of the activity with protocatechuate for the free enzyme, 150% for the calcium alginate-immobilized enzyme, and 97% for the glyoxyl agarose-immobilized enzyme
-
-
?
catechol + O2
cis,cis-muconate
-
reaction of EC 1.13.11.1
-
-
?
catechol + O2
cis,cis-muconate
-
reaction of EC 1.13.11.1
-
-
?
catechol + O2
muconate
-
-
-
?
catechol + O2
muconate
-
-
-
?
catechol + O2
muconate
-
at 3% the rate of protocatechuic acid oxidation
-
?
catechol + O2
muconate
-
-
-
-
?
catechol + O2
muconate
-
-
-
?
catechol + O2
muconate
-
-
-
?
catechol + O2
muconate
Nocardia erythropolis
-
at 33.8% the rate of protocatechuic acid oxidation
-
?
catechol + O2
muconate
-
at 0.4% the rate of protocatechuic acid oxidation
-
?
catechol + O2
muconate
-
at 0.4% the rate of protocatechuic acid oxidation
-
?
protocatechuate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
protocatechuate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
protocatechuate + O2
beta-carboxy-cis-cis-muconate
-
-
-
-
?
protocatechuate + O2
beta-carboxy-cis-cis-muconate
-
-
-
-
?
protocatechuate + O2
beta-carboxy-cis-cis-muconate
-
-
-
-
?
protocatechuate + O2
beta-carboxy-cis-cis-muconate
-
-
-
-
?
pyrogallol + O2
?
-
at 10% the rate of protocatechuic acid oxidation
-
-
?
pyrogallol + O2
?
Nocardia erythropolis
-
at 36% the rate of protocatechuic acid oxidation
-
-
?
pyrogallol + O2
?
-
at 0.4% the rate of protocatechuic acid oxidation
-
-
?
pyrogallol + O2
?
-
at 0.4% the rate of protocatechuic acid oxidation
-
-
?
pyrogallol + O2
?
-
-
-
-
?
pyrogallol + O2
?
-
-
-
-
?
trans-3,4-dihydroxycinnamate + O2
?
-
-
-
-
?
trans-3,4-dihydroxycinnamate + O2
?
-
W153V protocatechuate 3,4-dioxygenase I mutant enzyme: 35% activity compared to protocatechuate, wild-type protocatechuate 3,4-dioxygenase I: 2% activity compared to protocatechuate
-
-
?
trans-3,4-dihydroxycinnamate + O2
?
-
-
-
-
?
vanillic acid + O2
?
-
-
-
?
vanillic acid + O2
?
-
-
-
?
1.13.11.3 protocatechuate 3,4-dioxygenase
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