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IUBMB Comments The enzyme requires Fe(II) and ascorbate. Isolated from the bacterium Streptomyces avermitilis . Part of the pathway for pentalenolactone biosynthesis.
The enzyme appears in viruses and cellular organisms
Synonyms pntH , ptlH , sav2991 , more
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ptlH
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1-deoxypentalenate + 2-oxoglutarate + O2 = 1-deoxy-11beta-hydroxypentalenate + succinate + CO2
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MetaCyc
neopentalenoketolactone and pentalenate biosynthesis, pentalenolactone biosynthesis
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1-deoxypentalenic acid,2-oxoglutarate:oxygen oxidoreductase
The enzyme requires Fe(II) and ascorbate. Isolated from the bacterium Streptomyces avermitilis. Part of the pathway for pentalenolactone biosynthesis.
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(+/-)-1-deoxypentalenic acid + O2
11beta-hydroxy-1-deoxypentalenic acid + succinate + CO2
Substrates: - Products: -
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additional information
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additional information
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Substrates: no substrate: (+/-)-pentalenene, (+/-)-pentalen-13-ol Products: -
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additional information
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Substrates: no substrate: ent-1-deoxypentalenic acid Products: -
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Iron
non-heme iron, alpha-ketoglutarate-dependent hydroxylase
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0.57
(+/-)-1-deoxypentalenic acid
pH 6.0, temperature not specified in the publication
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4.2
(+/-)-1-deoxypentalenic acid
pH 6.0, temperature not specified in the publication
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UniProt
brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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PNTH_STRAE
283
0
31501
Swiss-Prot
-
PTLH_STRAW
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
285
0
32264
Swiss-Prot
-
PENH_STREX
283
0
31325
Swiss-Prot
-
A0A142XFP7_9BACT
260
0
29185
TrEMBL
-
A0A5B9MPT4_9BACT
279
0
31788
TrEMBL
-
A0A0S3PTY4_9BRAD
285
0
31940
TrEMBL
-
A0A5S9QL84_9GAMM
305
0
34913
TrEMBL
-
A0A5J4E172_9BACT
277
0
31262
TrEMBL
-
A0A517Z9J4_9PLAN
256
0
29072
TrEMBL
-
A0A1V5FGU3_9BACT
305
0
34991
TrEMBL
-
A0A2H5X4W0_9BACT
292
0
33587
TrEMBL
-
A0A142WQ22_9PLAN
265
0
29674
TrEMBL
-
A0A7K0CAQ7_9ACTN
Streptomyces smaragdinus
282
0
32042
TrEMBL
-
A0A517ME00_9BACT
261
0
29435
TrEMBL
-
A0A518DND1_9BACT
286
0
32347
TrEMBL
-
A0A1P8WR66_9PLAN
Fuerstiella marisgermanici
249
0
27816
TrEMBL
-
A0A5B9R6G7_9BACT
255
0
28861
TrEMBL
-
A0A2Z4AET2_9BACT
351
0
40660
TrEMBL
-
A0A142X346_9PLAN
250
0
28233
TrEMBL
-
A0A1Y5T405_9RHOB
299
0
33302
TrEMBL
-
A0A5P3AA39_9RHOB
276
0
30953
TrEMBL
-
A0A517T2S2_9BACT
255
0
28824
TrEMBL
-
A0A1X7AP16_9GAMM
312
0
35037
TrEMBL
-
A0A1X7BUT5_9RHOB
296
0
32668
TrEMBL
-
A0A517ZHS5_9PLAN
301
0
34071
TrEMBL
-
A0A165SNG3_9HYPH
301
0
33806
TrEMBL
-
A0A5S9Q127_9GAMM
278
0
32020
TrEMBL
-
A0A1H8KLI8_9HYPH
309
0
34051
TrEMBL
-
A0A1P8W9H7_9PLAN
Fuerstiella marisgermanici
264
0
29928
TrEMBL
-
A0A517R9C8_9PLAN
265
0
29929
TrEMBL
-
A0A2Z4ABE5_9BACT
325
0
37050
TrEMBL
-
A0A166BKW0_9HYPH
301
0
33786
TrEMBL
-
A0A517X4R4_9PLAN
283
0
32211
TrEMBL
-
A0A1P8WNC9_9PLAN
Fuerstiella marisgermanici
249
0
28229
TrEMBL
-
A0A518HPK0_9BACT
279
0
31736
TrEMBL
-
A0A517ZZG4_9PLAN
243
0
26788
TrEMBL
-
A0A2Z4AE56_9BACT
336
0
38599
TrEMBL
-
A0A1V5RZ23_9CHLR
302
0
34013
TrEMBL
-
A0A518I4U4_9PLAN
172
0
18701
TrEMBL
-
A0A1X6YB47_9RHOB
292
0
32635
TrEMBL
-
A0A1X6Z5W9_9RHOB
334
0
36964
TrEMBL
-
A0A1C0U8S2_9GAMM
327
0
37134
TrEMBL
-
A0A0F2C0N2_9MICO
282
0
31849
TrEMBL
-
A0A1Y5SE46_9RHOB
Roseovarius litorisediminis
297
0
32732
TrEMBL
-
A0A5C6C723_9BACT
257
0
29320
TrEMBL
-
A0A0N7M9D8_9RHOB
290
0
32150
TrEMBL
-
A0A3N6GJM9_9ACTN
295
0
33611
TrEMBL
-
A0A2Z4AJL5_9BACT
326
0
36831
TrEMBL
-
A0A2H5X5Y0_9BACT
295
0
33211
TrEMBL
-
A0A2P5VM21_9PROT
337
0
38597
TrEMBL
-
A0A517X6J0_9PLAN
265
0
30097
TrEMBL
-
A0A165V8K5_9HYPH
301
0
33688
TrEMBL
-
A0A517ZYL9_9PLAN
258
0
28606
TrEMBL
-
A0A517PZV3_9PLAN
243
0
26785
TrEMBL
-
A0A3N6ELP4_9ACTN
309
0
34628
TrEMBL
-
A0A5S9QNU4_9GAMM
305
0
34931
TrEMBL
-
A0A517R898_9PLAN
243
0
27078
TrEMBL
-
A0A517N4D6_9BACT
256
0
29399
TrEMBL
-
A0A7M4DGW0_9MICO
283
0
31939
TrEMBL
-
A0A2Z2NRI0_9GAMM
291
0
33017
TrEMBL
-
A0A518DHI8_9BACT
302
0
33695
TrEMBL
-
A0A347W879_9PROT
277
0
30781
TrEMBL
-
A0A517QMT2_9PLAN
262
0
29549
TrEMBL
-
A0A518CRY8_9PLAN
266
0
30425
TrEMBL
-
A0A100JR42_STRSC
306
0
33895
TrEMBL
-
A0A6V6ZRE6_9RHOB
294
0
32858
TrEMBL
-
A0A518AQ92_9BACT
249
0
28329
TrEMBL
-
A0A2S6RLD0_9PROT
348
0
39235
TrEMBL
-
A0A518DUS1_9BACT
247
0
27125
TrEMBL
-
A0A5B9QTH0_9BACT
Bythopirellula goksoeyrii
268
0
30423
TrEMBL
-
A0A5C6AZP9_9BACT
318
0
37042
TrEMBL
-
A0A5C6B2D1_9BACT
254
0
29117
TrEMBL
-
A0A518GPP0_9PLAN
273
0
31068
TrEMBL
-
A0A1V5RSS5_9CHLR
312
0
35952
TrEMBL
-
A0A1J5STZ1_9ZZZZ
275
0
30793
TrEMBL
other Location (Reliability: 1 )
A0A1V5FRU4_9BACT
282
0
32669
TrEMBL
-
A0A5C6BQX1_9PLAN
263
0
29843
TrEMBL
-
A0A517QRB4_9PLAN
245
0
27811
TrEMBL
-
A0A2S6RKK2_9PROT
280
0
32221
TrEMBL
-
A0A517V6M1_9PLAN
283
0
32260
TrEMBL
-
A0A518C807_9BACT
261
0
29552
TrEMBL
-
A0A5C6BM62_9PLAN
305
0
34273
TrEMBL
-
A0A2Z4AB95_9BACT
325
0
37134
TrEMBL
-
A0A517PTE4_9PLAN
261
0
30587
TrEMBL
-
A0A5C5XPD3_9PLAN
281
0
31669
TrEMBL
-
A0A1V5FR86_9BACT
289
0
33655
TrEMBL
-
A0A5C5WZ24_9BACT
257
0
29450
TrEMBL
-
A0A5B9Q365_9BACT
Bythopirellula goksoeyrii
240
0
26928
TrEMBL
-
A0A518GHI6_9BACT
260
0
29325
TrEMBL
-
A0A3Q9JLL4_9MICO
283
0
32020
TrEMBL
-
A0A517ZVP6_9PLAN
263
0
29862
TrEMBL
-
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37121
x * 37121, calculated, x * 37139, MALDI-TOF
37139
x * 37121, calculated, x * 37139, MALDI-TOF
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?
x * 37121, calculated, x * 37139, MALDI-TOF
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complexes with the cofactors iron, alpha-ketoglutarate, and the nonreactive enantiomer of the substrate, ent-1-deoxypentalenic acid, in four different crystal forms to up to 1.31 A resolution. The overall structure of PtlH forms a double-stranded barrel helix fold and the cofactor-binding site for iron and alpha-keto-glutarate is similar to other double-stranded barrel helix fold enzymes. Binding of the substrate enantiomer induces a reorganization of the monoclinic crystal lattice leading to a disorder-order transition of a C-terminal alpha-helix. The newly formed helix blocks the major access to the active site and effectively traps the bound substrate
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R117Q
complete loss of activity
R188Q
280fold decrease in activity
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expression in Escherichia coli
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You, Z.; Omura, S.; Ikeda, H.; Cane, D.E.
Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlH, a non-heme iron dioxygenase of Streptomyces avermitilis
J. Am. Chem. Soc.
128
6566-6567
2006
Streptomyces avermitilis (Q82IZ1)
brenda
You, Z.; Omura, S.; Ikeda, H.; Cane, D.E.; Jogl, G.
Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis
J. Biol. Chem.
282
36552-36560
2007
Streptomyces avermitilis (Q82IZ1)
brenda
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