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EC Tree
IUBMB Comments The enzyme requires Fe(II) and ascorbate. Isolated from the bacterium Streptomyces avermitilis. Part of the pathway for pentalenolactone biosynthesis.
The enzyme appears in viruses and cellular organisms
Synonyms
pntH ,
ptlH ,
sav2991 ,
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ptlH
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1-deoxypentalenate + 2-oxoglutarate + O2 = 1-deoxy-11beta-hydroxypentalenate + succinate + CO2
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1-deoxypentalenic acid,2-oxoglutarate:oxygen oxidoreductase
The enzyme requires Fe(II) and ascorbate. Isolated from the bacterium Streptomyces avermitilis. Part of the pathway for pentalenolactone biosynthesis.
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(+/-)-1-deoxypentalenic acid + O2
11beta-hydroxy-1-deoxypentalenic acid + succinate + CO2
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additional information
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additional information
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no substrate: (+/-)-pentalenene, (+/-)-pentalen-13-ol
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additional information
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no substrate: ent-1-deoxypentalenic acid
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?
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Iron
non-heme iron, alpha-ketoglutarate-dependent hydroxylase
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0.57
(+/-)-1-deoxypentalenic acid
pH 6.0, temperature not specified in the publication
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4.2
(+/-)-1-deoxypentalenic acid
pH 6.0, temperature not specified in the publication
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UniProt
brenda
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PNTH_STRAE
283
0
31501
Swiss-Prot
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PENH_STREX
283
0
31325
Swiss-Prot
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PTLH_STRAW
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
285
0
32264
Swiss-Prot
-
A0A5S9QL84_9GAMM
305
0
34913
TrEMBL
-
A0A5P3AA39_9RHOB
276
0
30953
TrEMBL
-
A0A0N7M9D8_9RHOB
290
0
32150
TrEMBL
-
A0A5B9Q365_9BACT
240
0
26928
TrEMBL
-
A0A2Z4ABE5_9BACT
325
0
37050
TrEMBL
-
A0A2S6RLD0_9PROT
348
0
39235
TrEMBL
-
A0A5S9Q127_9GAMM
278
0
32020
TrEMBL
-
A0A518GHI6_9BACT
260
0
29325
TrEMBL
-
A0A5B9R6G7_9BACT
255
0
28861
TrEMBL
-
A0A5S9Q1W7_9GAMM
396
0
45274
TrEMBL
-
A0A2Z4AJL5_9BACT
326
0
36831
TrEMBL
-
A0A1P8W9H7_9PLAN
264
0
29928
TrEMBL
-
A0A2P5VM21_9PROT
337
0
38597
TrEMBL
-
A0A142X346_9PLAN
250
0
28233
TrEMBL
-
A0A6V6ZRE6_9RHOB
294
0
32858
TrEMBL
-
A0A518DHI8_9BACT
302
0
33695
TrEMBL
-
A0A517R898_9BACT
243
0
27078
TrEMBL
-
A0A518CRY8_9BACT
266
0
30425
TrEMBL
-
A0A2H5X4W0_9BACT
292
0
33587
TrEMBL
-
A0A517PTE4_9BACT
261
0
30587
TrEMBL
other Location (Reliability: 2 )
A0A518DND1_9BACT
286
0
32347
TrEMBL
-
A0A518DUS1_9BACT
247
0
27125
TrEMBL
-
A0A3N6GJM9_9ACTN
295
0
33611
TrEMBL
-
A0A1V5RSS5_9CHLR
312
0
35952
TrEMBL
-
A0A518I4U4_9BACT
172
0
18701
TrEMBL
-
A0A1P8WNC9_9PLAN
249
0
28229
TrEMBL
-
A0A5B9QTH0_9BACT
268
0
30423
TrEMBL
-
A0A1X7BUT5_9RHOB
296
0
32668
TrEMBL
-
A0A1Y5T405_9RHOB
299
0
33302
TrEMBL
-
A0A2Z4AE56_9BACT
336
0
38599
TrEMBL
-
A0A5C6AZP9_9BACT
318
0
37042
TrEMBL
-
A0A2H5X5Y0_9BACT
295
0
33211
TrEMBL
-
A0A165V8K5_9RHOB
301
0
33688
TrEMBL
-
A0A1V5FGU3_9BACT
305
0
34991
TrEMBL
-
A0A517N4D6_9BACT
256
0
29399
TrEMBL
-
A0A517ZHS5_9BACT
301
0
34071
TrEMBL
-
A0A142WQ22_9PLAN
265
0
29674
TrEMBL
-
A0A5C6BM62_9BACT
305
0
34273
TrEMBL
-
A0A517V6M1_9BACT
283
0
32260
TrEMBL
-
A0A1H8KLI8_9RHIZ
309
0
34051
TrEMBL
-
A0A5C5XPD3_9PLAN
281
0
31669
TrEMBL
-
A0A518C807_9PLAN
261
0
29552
TrEMBL
-
A0A5C6BQX1_9BACT
263
0
29843
TrEMBL
-
A0A5J4E172_9BACT
277
0
31262
TrEMBL
-
A0A5C6B2D1_9BACT
254
0
29117
TrEMBL
-
A0A0F2C0N2_9MICO
282
0
31849
TrEMBL
-
A0A517X6J0_9BACT
265
0
30097
TrEMBL
other Location (Reliability: 1 )
A0A2Z2NRI0_9GAMM
291
0
33017
TrEMBL
-
A0A5C6C723_9BACT
257
0
29320
TrEMBL
-
A0A165SNG3_9RHOB
301
0
33806
TrEMBL
-
A0A1V5FRU4_9BACT
282
0
32669
TrEMBL
-
A0A5B9MPT4_9BACT
279
0
31788
TrEMBL
-
A0A1P8WR66_9PLAN
249
0
27816
TrEMBL
-
A0A517R9C8_9BACT
265
0
29929
TrEMBL
-
A0A2Z4AB95_9BACT
325
0
37134
TrEMBL
-
A0A518GPP0_9PLAN
273
0
31068
TrEMBL
-
A0A1V5RZ23_9CHLR
302
0
34013
TrEMBL
-
A0A1J5STZ1_9ZZZZ
275
0
30793
TrEMBL
other Location (Reliability: 1 )
A0A5C5WZ24_9BACT
257
0
29450
TrEMBL
-
A0A1C0U8S2_9GAMM
327
0
37134
TrEMBL
-
A0A100JR42_STRSC
306
0
33895
TrEMBL
-
A0A517Z9J4_9BACT
256
0
29072
TrEMBL
-
A0A518AQ92_9BACT
249
0
28329
TrEMBL
-
A0A517T2S2_9BACT
255
0
28824
TrEMBL
-
A0A1X7AP16_9GAMM
312
0
35037
TrEMBL
-
A0A3N6ELP4_9ACTN
309
0
34628
TrEMBL
-
A0A517ZYL9_9BACT
258
0
28606
TrEMBL
-
A0A2S6RKK2_9PROT
280
0
32221
TrEMBL
-
A0A1V5FR86_9BACT
289
0
33655
TrEMBL
-
A0A1X6YB47_9RHOB
292
0
32635
TrEMBL
-
A0A2Z4AET2_9BACT
351
0
40660
TrEMBL
-
A0A517X4R4_9BACT
283
0
32211
TrEMBL
other Location (Reliability: 2 )
A0A347W879_9PROT
277
0
30781
TrEMBL
-
A0A518HPK0_9BACT
279
0
31736
TrEMBL
-
A0A517ZZG4_9BACT
243
0
26788
TrEMBL
other Location (Reliability: 1 )
A0A3Q9JLL4_9MICO
283
0
32020
TrEMBL
-
A0A517ZVP6_9BACT
263
0
29862
TrEMBL
-
A0A517ME00_9BACT
261
0
29435
TrEMBL
-
A0A1X6Z5W9_9RHOB
334
0
36964
TrEMBL
-
A0A166BKW0_9RHOB
301
0
33786
TrEMBL
-
A0A0S3PTY4_9BRAD
285
0
31940
TrEMBL
-
A0A1Y5SE46_9RHOB
297
0
32732
TrEMBL
-
A0A5S9QNU4_9GAMM
305
0
34931
TrEMBL
-
A0A142XFP7_9BACT
260
0
29185
TrEMBL
-
A0A517PZV3_9BACT
243
0
26785
TrEMBL
-
A0A517QMT2_9BACT
262
0
29549
TrEMBL
-
A0A517QRB4_9BACT
245
0
27811
TrEMBL
-
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37121
x * 37121, calculated, x * 37139, MALDI-TOF
37139
x * 37121, calculated, x * 37139, MALDI-TOF
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?
x * 37121, calculated, x * 37139, MALDI-TOF
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complexes with the cofactors iron, alpha-ketoglutarate, and the nonreactive enantiomer of the substrate, ent-1-deoxypentalenic acid, in four different crystal forms to up to 1.31 A resolution. The overall structure of PtlH forms a double-stranded barrel helix fold and the cofactor-binding site for iron and alpha-keto-glutarate is similar to other double-stranded barrel helix fold enzymes. Binding of the substrate enantiomer induces a reorganization of the monoclinic crystal lattice leading to a disorder-order transition of a C-terminal alpha-helix. The newly formed helix blocks the major access to the active site and effectively traps the bound substrate
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R117Q
complete loss of activity
R188Q
280fold decrease in activity
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expression in Escherichia coli
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You, Z.; Omura, S.; Ikeda, H.; Cane, D.E.
Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlH, a non-heme iron dioxygenase of Streptomyces avermitilis
J. Am. Chem. Soc.
128
6566-6567
2006
Streptomyces avermitilis (Q82IZ1)
brenda
You, Z.; Omura, S.; Ikeda, H.; Cane, D.E.; Jogl, G.
Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis
J. Biol. Chem.
282
36552-36560
2007
Streptomyces avermitilis (Q82IZ1)
brenda
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Enzyme Nomenclature
1.14.11.35 1-deoxypentalenic acid 11beta-hydroxylase
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