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Information on EC 1.14.13.20 - 2,4-dichlorophenol 6-monooxygenase
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EC Tree
1.14.13.20 2,4-dichlorophenol 6-monooxygenaseIUBMB Comments
A flavoprotein (FAD). Also acts, more slowly, on 4-chlorophenol and 4-chloro-2-methylphenol; NADH can act instead of NADPH, but more slowly.
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Word Map
- 1.14.13.20
-
2,4-dichlorophenoxyacetic
- chlorocatechols
- 3,5-dichlorocatechol
- fad
- chlorophenols
- catechols
-
alcaligenes
- eutrophus
-
2,4-d-degradative
-
ortho-substituted
- 3-chlorophenol
- maleylacetate
- alpha-2
-
polychlorinated
- 4-chlorocatechol
-
cycloisomerase
-
biphenyl-contaminated
-
chemotaxonomic
-
3-chloro
-
chloromuconate
-
non-substrate
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
2,4-dichlorophenol hydroxylase, tfdb-jlu, 2,4-dcp hydroxylase, chlorophenol hydroxylase, 2,4-dichlorophenol 6-monooxygenase, 2,4-dichlorophenol monooxygenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2,4-dichlorophenol hydroxylase
2,4-dichlorophenol monooxygenase
-
-
-
-
chlorophenol hydroxylase
DCM
oxygenase, 2,4-dichlorophenol 6-mono
-
-
-
-
oxygenase, 2,4-dichlorophenol mono-
-
-
-
-
phenol hydroxylase
TfdB-JLU
2,4-dichlorophenol hydroxylase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2,4-dichlorophenol + NADPH + H+ + O2 = 3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
-
,
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SYSTEMATIC NAME
IUBMB Comments
2,4-dichlorophenol,NADPH:oxygen oxidoreductase (6-hydroxylating)
A flavoprotein (FAD). Also acts, more slowly, on 4-chlorophenol and 4-chloro-2-methylphenol; NADH can act instead of NADPH, but more slowly.
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CAS REGISTRY NUMBER
COMMENTARY
82047-82-3
-
92767-55-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,3-dichlorophenol + NADPH + O2
3,4-dichlorocatechol + NADP+ + H2O
Soil bacterium
-
113% of the activity with 2,4-dichlorophenol
-
-
?
2,4,5-trichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
-
low activity
-
-
?
2,5-dichlorophenol + NADPH + O2
3,6-dichlorocatechol + NADP+ + H2O
Soil bacterium
-
103% of the activity with 2,4-dichlorophenol
-
-
?
2,5-dichlorophenol + NADPH + O2
? + NADP+ + H2O
Soil bacterium
-
178% of the activity with 2,4-dichlorophenol
-
-
?
2-bromo-4-chlorophenol + NADPH + O2
3-bromo-5-chlorocatechol + NADP+ + H2O
Soil bacterium
-
82% of the activity with 2,4-dichlorophenol
-
-
?
2-chlorophenol + NADPH + O2
3-chlorocatechol + NADP+ + H2O
Soil bacterium
-
129% of the activity with 2,4-dichlorophenol
-
-
?
3,5-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
-
low activity
-
-
?
3-chlorophenol + NADPH + O2
4-chlorocatechol + NADP+ + H2O
Soil bacterium
-
230% of the activity with 2,4-dichlorophenol
-
-
?
4-bromo-2-chlorophenol + NADPH + O2
5-bromo-3-chlorocatechol + NADP+ + H2O
Soil bacterium
-
86% of the activity with 2,4-dichlorophenol
-
-
?
4-chloro-2-methylphenol + NADPH + O2
5-chloro-3-methylcatechol + NADP+ + H2O
Soil bacterium
-
95% of the activity with 2,4-dichlorophenol
-
-
?
2,3-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
26% activity compared to 2,4-dichlorophenol as substrate
-
-
?
2,3-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
26% activity compared to 2,4-dichlorophenol as substrate
-
-
?
2,4-dichlorophenol + NADH + H+ + O2
3,5-dichlorocatechol + NAD+ + H2O
-
-
-
-
r
2,4-dichlorophenol + NADH + H+ + O2
3,5-dichlorocatechol + NAD+ + H2O
-
-
-
-
r
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
Bacillus licheniformis MTCC 25059
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
Soil bacterium
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
Proteobacteria S1
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
30% activity compared to 2,4-dichlorophenol as substrate
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
30% activity compared to 2,4-dichlorophenol as substrate
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
-
low activity
-
-
?
4-chlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
68% activity compared to 2,4-dichlorophenol as substrate
-
-
?
4-chlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
68% activity compared to 2,4-dichlorophenol as substrate
-
-
?
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
-
-
-
-
?
additional information
?
-
-
pseudosubstrates evoke oxidation of NAD(P)H and oxygen consumption without themselves undergoing hydroxylation, the product is hydrogen peroxide: 2,5-dichlorophenol, 2,6-dichlorophenol, 3,4-dichlorophenol, 2, 4,5-trichlorophenol
-
-
?
additional information
?
-
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
-
-
?
additional information
?
-
-
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
-
-
?
additional information
?
-
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
-
-
?
additional information
?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
-
-
?
additional information
?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
-
-
?
additional information
?
-
-
the 2,4-DCP hydroxylase (TfdB-JLU) exhibits broad substrate specificity for chlorophenols (CPs) and their homologues. Substrate specificity of wild-type and mutant P316Q enzymes, overview
-
-
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
Bacillus licheniformis MTCC 25059
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
Proteobacteria S1
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
-
-
-
-
?
additional information
?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
-
-
?
additional information
?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KCl
-
high salt concentration inhibits, 0.05 M: 40%, 0.1 M: 50%, 0.2 M: 70%