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1,1-diethylcyclopropane + NADH + H+ + O2
? + NAD+ + H2O
1,1-dimethylcyclopropane + NADH + H+ + O2
? + NAD+ + H2O
2 fluorobenzene + NADH + H+ + O2
4-fluorophenol + 4-fluorocatechol + NAD+ + H2O
2 indole + 3 NADH + 3 H+ + 3 O2
indirubin + 3 NAD+ + 3 H2O
A0A0D3QM77; A0A0D3QME2; A0A0D3QLU4; A0A0D3QM47; A0A0D3QMJ7; A0A0D3QM80
-
reaction via C-3 oxidation to indoxyl, oxidation to isatin, and recombinantion of isatin and indoxyl to indirubin
-
?
2-phenylethanol + 2 NADH + 2 H+ + O2
3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
2-phenylethanol + NADH + H+ + O2
m-tyrosol + p-tyrosol + NAD+ + H2O
2-phenylethanol + NADH + H+ + O2
o-tyrosol + m-tyrosol + NAD+ + H2O
2-xylene + NADH + H+ + O2
3,4-dimethylphenol + NAD+ + H2O
3-xylene + NADH + H+ + O2
2,4-dimethylphenol + NAD+ + H2O
4-xylene + NADH + H+ + O2
2,5-dimethylphenol + 4-methyl benzyl alcohol + NAD+ + H2O
anisole + NADH + H+ + O2
4-methoxyphenol + NAD+ + H2O
benzene + NADH + H+ + O2
phenol + NAD+ + H2O
chlorobenzene + NADH + H+ + O2
4-chlorophenol + NAD+ + H2O
m-cresol + NADH + H+ + O2
4-methylcatechol + NAD+ + H2O
m-tyrosol + 2 NADH + 2 H+ + O2
2,3-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
m-tyrosol + 2 NADH + 2 H+ + O2
3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
methoxybenzene + NADH + H+ + O2
4-methoxyphenol + NAD+ + H2O
-
more than 99% 4-methoxyphenol
-
?
methyl 4-tolyl sulfide + NADH + H+ + O2
methyl 4-tolyl sulfoxide + NAD+ + H2O
methyl p-tolyl sulfide + NADH + H+ + O2
methyl p-tolyl sulfoxide + NAD+ + H2O
methyl phenyl sulfide + NADH + H+ + O2
methyl phenyl sulfoxide + NAD+ + H2O
naphthalene + NADH + H+ + O2
2-naphthol + NAD+ + H2O
nitrobenzene + NADH + H+ + O2
4-nitrophenol + NAD+ + H2O
norcarane + NADH + H+ + O2
endo-2-norcaranol + exo-2-norcaranol + endo-3-norcaranol + NAD+ + H2O
o-cresol + NADH + H+ + O2
3-methylcatechol + NAD+ + H2O
o-methoxyphenol + NADH + H+ + O2
4-methoxyresorcinol + 3-methoxycatechol + NAD+ + H2O
o-tyrosol + 2 NADH + 2 H+ + O2
2,3-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
o-tyrosol + NADH + H+ + O2
2,3-dihydroxyphenylethanol + NAD+ + H2O
p-cresol + NADH + H+ + O2
4-methylcatechol + NAD+ + H2O
p-tyrosol + 2 NADH + 2 H+ + O2
3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
phenylethanol + NADH + H+ + O2
m-tyrosol + p-tyrosol + NAD+ + H2O
styrene + NADH + H+ + O2
styrene epoxide + NAD+ + H2O
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
toluene + NADH + H+ + O2
p-cresol + NAD+ + H2O
additional information
?
-
1,1-diethylcyclopropane + NADH + H+ + O2
? + NAD+ + H2O
-
-
-
?
1,1-diethylcyclopropane + NADH + H+ + O2
? + NAD+ + H2O
-
-
-
?
1,1-dimethylcyclopropane + NADH + H+ + O2
? + NAD+ + H2O
-
-
-
?
1,1-dimethylcyclopropane + NADH + H+ + O2
? + NAD+ + H2O
-
-
-
?
2 fluorobenzene + NADH + H+ + O2
4-fluorophenol + 4-fluorocatechol + NAD+ + H2O
-
whole-cell reaction, the predominant product is either 4-fluorophenol or 4-fluorocatechol depending on the ratio of biocatalyst to substrate concentration
-
?
2 fluorobenzene + NADH + H+ + O2
4-fluorophenol + 4-fluorocatechol + NAD+ + H2O
-
whole-cell reaction, the predominant product is either 4-fluorophenol or 4-fluorocatechol depending on the ratio of biocatalyst to substrate concentration
-
?
2-phenylethanol + 2 NADH + 2 H+ + O2
3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
-
reaction of mutants I100A, I100S, I100G
-
?
2-phenylethanol + 2 NADH + 2 H+ + O2
3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
-
reaction of mutants I100A, I100S, I100G
-
?
2-phenylethanol + NADH + H+ + O2
m-tyrosol + p-tyrosol + NAD+ + H2O
-
63% m-tyosol + 37% p-tyrosol
-
?
2-phenylethanol + NADH + H+ + O2
m-tyrosol + p-tyrosol + NAD+ + H2O
-
63% m-tyosol + 37% p-tyrosol
-
?
2-phenylethanol + NADH + H+ + O2
o-tyrosol + m-tyrosol + NAD+ + H2O
-
64% o-tyrosol + 36% m-tyrosol
-
?
2-phenylethanol + NADH + H+ + O2
o-tyrosol + m-tyrosol + NAD+ + H2O
-
64% o-tyrosol + 36% m-tyrosol
-
?
2-xylene + NADH + H+ + O2
3,4-dimethylphenol + NAD+ + H2O
-
95% 3,4-dimethylphenol plus 5% 2-methyl benzyl alcohol
-
?
2-xylene + NADH + H+ + O2
3,4-dimethylphenol + NAD+ + H2O
-
95% 3,4-dimethylphenol plus 5% 2-methyl benzyl alcohol
-
?
3-xylene + NADH + H+ + O2
2,4-dimethylphenol + NAD+ + H2O
-
97% 2,4-dimethylphenol plus 2.5% 3-methyl benzyl alcohol plus 0.5% 3,5-dimethylphenol
-
?
3-xylene + NADH + H+ + O2
2,4-dimethylphenol + NAD+ + H2O
-
97% 2,4-dimethylphenol plus 2.5% 3-methyl benzyl alcohol plus 0.5% 3,5-dimethylphenol
-
?
4-xylene + NADH + H+ + O2
2,5-dimethylphenol + 4-methyl benzyl alcohol + NAD+ + H2O
-
82% 2,5-dimethylphenol plus 18% 4-methyl benzyl alcohol
-
?
4-xylene + NADH + H+ + O2
2,5-dimethylphenol + 4-methyl benzyl alcohol + NAD+ + H2O
-
82% 2,5-dimethylphenol plus 18% 4-methyl benzyl alcohol
-
?
anisole + NADH + H+ + O2
4-methoxyphenol + NAD+ + H2O
-
87% efficiency
-
?
anisole + NADH + H+ + O2
4-methoxyphenol + NAD+ + H2O
-
87% efficiency
-
?
benzene + NADH + H+ + O2
phenol + NAD+ + H2O
-
-
-
?
benzene + NADH + H+ + O2
phenol + NAD+ + H2O
-
sole product
-
?
benzene + NADH + H+ + O2
phenol + NAD+ + H2O
-
98% efficiency
-
?
benzene + NADH + H+ + O2
phenol + NAD+ + H2O
-
-
-
?
benzene + NADH + H+ + O2
phenol + NAD+ + H2O
-
98% efficiency
-
?
benzene + NADH + H+ + O2
phenol + NAD+ + H2O
-
sole product
-
?
chlorobenzene + NADH + H+ + O2
4-chlorophenol + NAD+ + H2O
-
39% efficiency
-
?
chlorobenzene + NADH + H+ + O2
4-chlorophenol + NAD+ + H2O
-
more than 95% 4-chlorophenol
-
?
chlorobenzene + NADH + H+ + O2
4-chlorophenol + NAD+ + H2O
-
39% efficiency
-
?
m-cresol + NADH + H+ + O2
4-methylcatechol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
100% 4-methylcatechol
-
?
m-cresol + NADH + H+ + O2
4-methylcatechol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
100% 4-methylcatechol
-
?
m-tyrosol + 2 NADH + 2 H+ + O2
2,3-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
-
9% 2,3-dihydroxyphenylethanol
-
?
m-tyrosol + 2 NADH + 2 H+ + O2
2,3-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
-
9% 2,3-dihydroxyphenylethanol
-
?
m-tyrosol + 2 NADH + 2 H+ + O2
3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
-
mutants I100A, I100S, I100D, I100V and I100G hydroxylate m-tyrosol to form 3,4-dihydroxyphenylethanol with conversion of 55%, 50%, 2%, 3% and 18%, respectively
-
?
m-tyrosol + 2 NADH + 2 H+ + O2
3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
-
mutants I100A, I100S, I100D, I100V and I100G hydroxylate m-tyrosol to form 3,4-dihydroxyphenylethanol with conversion of 55%, 50%, 2%, 3% and 18%, respectively
-
?
methyl 4-tolyl sulfide + NADH + H+ + O2
methyl 4-tolyl sulfoxide + NAD+ + H2O
-
(pro-S)-sulfoxide, 11% enantiomeric excess
-
?
methyl 4-tolyl sulfide + NADH + H+ + O2
methyl 4-tolyl sulfoxide + NAD+ + H2O
-
(pro-S)-sulfoxide, 11% enantiomeric excess
-
?
methyl 4-tolyl sulfide + NADH + H+ + O2
methyl 4-tolyl sulfoxide + NAD+ + H2O
-
(pro-R)-sulfoxide, 41% enantiomeric excess
-
?
methyl 4-tolyl sulfide + NADH + H+ + O2
methyl 4-tolyl sulfoxide + NAD+ + H2O
-
(pro-R)-sulfoxide, 41% enantiomeric excess
-
?
methyl p-tolyl sulfide + NADH + H+ + O2
methyl p-tolyl sulfoxide + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
-
-
?
methyl p-tolyl sulfide + NADH + H+ + O2
methyl p-tolyl sulfoxide + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
-
-
?
methyl phenyl sulfide + NADH + H+ + O2
methyl phenyl sulfoxide + NAD+ + H2O
-
(pro-S)-sulfoxide, 51% enantiomeric excess
-
?
methyl phenyl sulfide + NADH + H+ + O2
methyl phenyl sulfoxide + NAD+ + H2O
-
(pro-S)-sulfoxide, 51% enantiomeric excess
-
?
methyl phenyl sulfide + NADH + H+ + O2
methyl phenyl sulfoxide + NAD+ + H2O
-
(pro-S)-sulfoxide, 86% enantiomeric excess
-
?
methyl phenyl sulfide + NADH + H+ + O2
methyl phenyl sulfoxide + NAD+ + H2O
-
(pro-S)-sulfoxide, 86% enantiomeric excess
-
?
naphthalene + NADH + H+ + O2
2-naphthol + NAD+ + H2O
-
-
-
?
naphthalene + NADH + H+ + O2
2-naphthol + NAD+ + H2O
-
-
-
?
nitrobenzene + NADH + H+ + O2
4-nitrophenol + NAD+ + H2O
-
12% efficiency
-
?
nitrobenzene + NADH + H+ + O2
4-nitrophenol + NAD+ + H2O
-
88% 4-nitrophenol plus 9% 3-nitrophenol plus 3% 2-nitrophenol
-
?
nitrobenzene + NADH + H+ + O2
4-nitrophenol + NAD+ + H2O
-
12% efficiency
-
?
norcarane + NADH + H+ + O2
endo-2-norcaranol + exo-2-norcaranol + endo-3-norcaranol + NAD+ + H2O
-
72% coupling, 47.5% endo-2-norcaranol, 39.2% exo-2- and endo-3-norcaranol, 8.8% exo-3-norcaranol
-
?
norcarane + NADH + H+ + O2
endo-2-norcaranol + exo-2-norcaranol + endo-3-norcaranol + NAD+ + H2O
-
72% coupling, 47.5% endo-2-norcaranol, 39.2% exo-2- and endo-3-norcaranol, 8.8% exo-3-norcaranol
-
?
o-cresol + NADH + H+ + O2
3-methylcatechol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
91% 3-methylcatechol + 9% methylhydroquinone
-
?
o-cresol + NADH + H+ + O2
3-methylcatechol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
91% 3-methylcatechol + 9% methylhydroquinone
-
?
o-methoxyphenol + NADH + H+ + O2
4-methoxyresorcinol + 3-methoxycatechol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
87% 4-methoxyresorcinol, 11% 3-methoxycatechol, and 2% methoxyhydroquinone
-
?
o-methoxyphenol + NADH + H+ + O2
4-methoxyresorcinol + 3-methoxycatechol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
87% 4-methoxyresorcinol, 11% 3-methoxycatechol, and 2% methoxyhydroquinone
-
?
o-tyrosol + 2 NADH + 2 H+ + O2
2,3-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
-
mutants I100A, I100S and I100G hydroxylate p-tyrosol to form 3,4-dihydroxyphenylethanol with 24%, 11% and 13% conversion, respectively, respectively
-
?
o-tyrosol + 2 NADH + 2 H+ + O2
2,3-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
-
mutants I100A, I100S and I100G hydroxylate p-tyrosol to form 3,4-dihydroxyphenylethanol with 24%, 11% and 13% conversion, respectively, respectively
-
?
o-tyrosol + NADH + H+ + O2
2,3-dihydroxyphenylethanol + NAD+ + H2O
-
83% 2,3-dihydroxyphenylethanol
-
?
o-tyrosol + NADH + H+ + O2
2,3-dihydroxyphenylethanol + NAD+ + H2O
-
83% 2,3-dihydroxyphenylethanol
-
?
o-tyrosol + NADH + H+ + O2
2,3-dihydroxyphenylethanol + NAD+ + H2O
reaction of mutant S395C, 18% conversion in 44 h
-
-
?
p-cresol + NADH + H+ + O2
4-methylcatechol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
100% 4-methylcatechol
-
?
p-cresol + NADH + H+ + O2
4-methylcatechol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
100% 4-methylcatechol
-
?
p-tyrosol + 2 NADH + 2 H+ + O2
3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
-
10% 3,4-dihydroxyphenylethanol
-
?
p-tyrosol + 2 NADH + 2 H+ + O2
3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
-
10% 3,4-dihydroxyphenylethanol
-
?
p-tyrosol + 2 NADH + 2 H+ + O2
3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O
-
mutants I100A, I100S and I100G hydroxylate p-tyrosol to form 3,4-dihydroxyphenylethanol with 65%, 48% and 98% conversion, respectively, respectively
-
?
phenylethanol + NADH + H+ + O2
m-tyrosol + p-tyrosol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
63% m-tyrosol + 37% p-tyrosol
-
?
phenylethanol + NADH + H+ + O2
m-tyrosol + p-tyrosol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
63% m-tyrosol + 37% p-tyrosol
-
?
styrene + NADH + H+ + O2
styrene epoxide + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
-
-
?
styrene + NADH + H+ + O2
styrene epoxide + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
-
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
-
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
-
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
-
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
-
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
-
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
194% efficiency
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
94% coupling
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
96% 4-methylphenol + 3% 3-methylphenol
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
96% 4-methylphenol plus 2.8% 3-methylphenol plus 0.4% 2-methylphenol
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
96% 4-methylphenol plus 2.8% 3-methylphenol plus 0.4% 2-methylphenol plus 0.8% benzyl alcohol
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
96.2% 4-methylphenol, 1.5% 3-methylphenol, 0.9% 2-methylphenol, 1.4% benzyl alcohol
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
-
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
96% 4-methylphenol plus 2.8% 3-methylphenol plus 0.4% 2-methylphenol plus 0.8% benzyl alcohol
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
194% efficiency
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
-
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
94% coupling
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
-
-
-
?
toluene + NADH + H+ + O2
4-methylphenol + NAD+ + H2O
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
-
96% 4-methylphenol + 3% 3-methylphenol
-
?
toluene + NADH + H+ + O2
p-cresol + NAD+ + H2O
-
-
-
?
toluene + NADH + H+ + O2
p-cresol + NAD+ + H2O
-
-
-
?
additional information
?
-
no substrates for wild-type: o-tyrosol, m-tyrosol, p-tyrosol
-
-
?
additional information
?
-
no substrates for wild-type: o-tyrosol, m-tyrosol, p-tyrosol
-
-
?
additional information
?
-
experiments with p-deuterotoluene lead to the isolation of p-cresol which retains 68% of the deuterium initially present in the parent molecule. When incubated with toluene in the presence of 1802, the oxygen in p-cresol is derived from molecular oxygen
-
-
?
additional information
?
-
highly specific for para hydroxylation of toluene, o-xylene, m-xylene
-
-
?
additional information
?
-
no substrates for wild-type: o-tyrosol, m-tyrosol, p-tyrosol
-
-
?
additional information
?
-
products are consistent with both radical rearrangement and cation ring expansion. Products show high-fidelity incorporation of an O-atom from O2 in the un-rearranged and radical-rearranged products, while the O-atom found in the cation ring-expansion products is predominantly obtained by reaction with H2O
-
-
?
additional information
?
-
highly specific for para hydroxylation of toluene, o-xylene, m-xylene
-
-
?
additional information
?
-
-
highly specific for para hydroxylation of toluene, o-xylene, m-xylene
-
-
?
additional information
?
-
no substrates for wild-type: o-tyrosol, m-tyrosol, p-tyrosol
-
-
?
additional information
?
-
products are consistent with both radical rearrangement and cation ring expansion. Products show high-fidelity incorporation of an O-atom from O2 in the un-rearranged and radical-rearranged products, while the O-atom found in the cation ring-expansion products is predominantly obtained by reaction with H2O
-
-
?
additional information
?
-
experiments with p-deuterotoluene lead to the isolation of p-cresol which retains 68% of the deuterium initially present in the parent molecule. When incubated with toluene in the presence of 1802, the oxygen in p-cresol is derived from molecular oxygen
-
-
?
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V106A
initial thioanisole sulfoxidation is improved by 1.65fold
V106E
initial thioanisole sulfoxidation is improved by 1.72fold
V106L
initial thioanisole sulfoxidation is decreased by 0.43fold
V106M
mutant oxidizes methyl phenyl sulfide to the corresponding sulfoxide at a rate of 3.0 nmol/min/mg protein compared with 1.6 for the wild-type enzyme, and the enantiomeric excess (pro-S) increases from 51% for the wild type to 88% for this mutant. Function of residue V106 is the proper positioning or docking of the substrate with respect to the diiron atoms
V106S
initial thioanisole sulfoxidation is decreased by 0.8fold
V106A
-
initial thioanisole sulfoxidation is improved by 1.65fold
-
V106E
-
initial thioanisole sulfoxidation is improved by 1.72fold
-
V106L
-
initial thioanisole sulfoxidation is decreased by 0.43fold
-
V106M
-
mutant oxidizes methyl phenyl sulfide to the corresponding sulfoxide at a rate of 3.0 nmol/min/mg protein compared with 1.6 for the wild-type enzyme, and the enantiomeric excess (pro-S) increases from 51% for the wild type to 88% for this mutant. Function of residue V106 is the proper positioning or docking of the substrate with respect to the diiron atoms
-
V106S
-
initial thioanisole sulfoxidation is decreased by 0.8fold
-
D285A
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 2.7fold increase in activity with 2-phenylethanol
D285C
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 4fold increase in activity with 2-phenylethanol
D285I
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 6.6fold increase in activity with 2-phenylethanol
D285L
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 5.4fold increase in activity with 2-phenylethanol
D285P
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 3.3fold increase in activity with 2-phenylethanol
D285Q
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 10.5fold increase in activity with 2-phenylethanol
D285S
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 70% of wild-type activity
D285Y mutation in subunit TmoA,
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
3fold increase in activity with 2-phenylethanol
F205I
decrease in regiospecificity for p-cresol formation, about 5-fold increase in the percentage of m-cresol formation. Mutant gives nearly equivalent amounts of benzylic and phenolic products from p-xylene oxidation
G103A/A107S
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, produces 3-methylcatechol (98%) from o-cresol twofold faster and produces 3-methoxycatechol (82%) from 1mM o-methoxyphenol seven times faster than the wild-type
G103S
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, produces 40fold more methoxyhydroquinone from o-methoxyphenol than the wild-type
G103S/A107T
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, produces methylhydroquinone (92%) from o-cresol fourfold faster than wild-type
I100A/D285I
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 52fold increase in activity with 2-phenylethanol
I100A/D285Q
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 85fold increase in activity with 2-phenylethanol
I100G/D285I
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 14.1fold increase in activity with methyl p-tolyl sulfide
I100L/D285S
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 1.4fold increase in activity with styrene
Q141C
decrease in regiospecificity for p-cresol formation, mutant functions predominantly as an aromatic ring hydroxylase during the oxidation of p-xylene
S395C
mutation in subunit TmoA, shows a 15fold increase in 2-phenylethanol hydroxylation rate
T201F
mutation causes a substantial shift in the product distribution, and gives o- and p-cresol in a 1:1 ratio
T201K
decrease in activity
T201L
parameters similar to wild-type
D285A
-
mutation in subunit TmoA, 2.7fold increase in activity with 2-phenylethanol
-
D285P
-
mutation in subunit TmoA, 3.3fold increase in activity with 2-phenylethanol
-
F205I
-
decrease in regiospecificity for p-cresol formation, about 5-fold increase in the percentage of m-cresol formation. Mutant gives nearly equivalent amounts of benzylic and phenolic products from p-xylene oxidation
-
G103A/A107S
-
mutation in subunit TmoA, produces 3-methylcatechol (98%) from o-cresol twofold faster and produces 3-methoxycatechol (82%) from 1mM o-methoxyphenol seven times faster than the wild-type
-
G103S
-
mutation in subunit TmoA, produces 40fold more methoxyhydroquinone from o-methoxyphenol than the wild-type
-
G103S/A107T
-
mutation in subunit TmoA, produces methylhydroquinone (92%) from o-cresol fourfold faster than wild-type
-
I100D
-
mutation improves both reaction rate and enantioselectivity
-
Q141C
-
decrease in regiospecificity for p-cresol formation, mutant functions predominantly as an aromatic ring hydroxylase during the oxidation of p-xylene
-
S395C
-
mutation in subunit TmoA, shows a 15fold increase in 2-phenylethanol hydroxylation rate
-
T201F
-
mutation causes a substantial shift in the product distribution, and gives o- and p-cresol in a 1:1 ratio
-
T201K
-
decrease in activity
-
T201L
-
parameters similar to wild-type
-
I100A
mutant hydroxylates o-tyrosol, m-tyrosol and p-tyrosol to form hydroxytyrosol
I100A
mutant shows similar rates as wild-type
I100A
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 35fold increase in activity with 2-phenylethanol
I100D
mutant hydroxylates m-tyrosol to form hydroxytyrosol
I100D
mutation improves both reaction rate and enantioselectivity
I100G
mutant hydroxylates o-tyrosol, m-tyrosol and p-tyrosol to form hydroxytyrosol
I100G
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 11fold increase in activity with methyl p-tolyl sulfide
I100G
mutation increases the wild-type oxidation rate of methyl phenyl sulfide by 1.7fold, and the enantiomeric excess rises from 86% to 98% pro-S. I100G oxidizes methyl para-tolyl sulfide 11 times faster than the wild type does and changes the selectivity from 41% pro-R to 77% pro-S
I100L
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, 0.9fold decrease in activity with styrene
I100L
Q6Q8Q7; Q6Q8Q6; Q6Q8Q5; Q6Q8Q4; Q6Q8Q3; Q6Q8Q2
mutation in subunit TmoA, produces 3-methoxycatechol from o-methoxyphenol four times faster than wild-type
I100S
mutant hydroxylates o-tyrosol, m-tyrosol and p-tyrosol to form hydroxytyrosol
I100S
mutation improves both reaction rate and enantioselectivity
I100V
mutant hydroxylates m-tyrosol to form hydroxytyrosol
I100V
mutation improves both reaction rate and enantioselectivity
T201A
mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution
T201A
mutation has no impact on steady-state catalysis or coupling. Mutant T201A gives stoichometric release of H2O2 during reaction in the absence of substrate and has a faster first-order rate constant for product formation than wild-type
T201A
parameters similar to wild-type
T201G
mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution
T201G
mutation has no impact on steady-state catalysis or coupling
T201S
mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution
T201S
mutation has no impact on steady-state catalysis or coupling
T201S
parameters similar to wild-type
I100A
-
mutant shows similar rates as wild-type
-
I100A
-
mutant hydroxylates o-tyrosol, m-tyrosol and p-tyrosol to form hydroxytyrosol
-
I100A
-
mutation in subunit TmoA, 35fold increase in activity with 2-phenylethanol
-
I100G
-
mutation increases the wild-type oxidation rate of methyl phenyl sulfide by 1.7fold, and the enantiomeric excess rises from 86% to 98% pro-S. I100G oxidizes methyl para-tolyl sulfide 11 times faster than the wild type does and changes the selectivity from 41% pro-R to 77% pro-S
-
I100G
-
mutant hydroxylates o-tyrosol, m-tyrosol and p-tyrosol to form hydroxytyrosol
-
I100G
-
mutation in subunit TmoA, 11fold increase in activity with methyl p-tolyl sulfide
-
I100L
-
mutation in subunit TmoA, produces 3-methoxycatechol from o-methoxyphenol four times faster than wild-type
-
I100L
-
mutation in subunit TmoA, 0.9fold decrease in activity with styrene
-
I100S
-
mutation improves both reaction rate and enantioselectivity
-
I100S
-
mutant hydroxylates o-tyrosol, m-tyrosol and p-tyrosol to form hydroxytyrosol
-
I100V
-
mutation improves both reaction rate and enantioselectivity
-
I100V
-
mutant hydroxylates m-tyrosol to form hydroxytyrosol
-
T201A
-
mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution
-
T201A
-
mutation has no impact on steady-state catalysis or coupling. Mutant T201A gives stoichometric release of H2O2 during reaction in the absence of substrate and has a faster first-order rate constant for product formation than wild-type
-
T201A
-
parameters similar to wild-type
-
T201G
-
mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution
-
T201G
-
mutation has no impact on steady-state catalysis or coupling
-
T201S
-
mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution
-
T201S
-
mutation has no impact on steady-state catalysis or coupling
-
T201S
-
parameters similar to wild-type
-
additional information
construction of variants with either four (DELTAN4-) seven (DELTAN7-), or 10 (DELTAN10-) residues removed from the N-terminal. Removal leads to statistically insignificant changes in kcat, KM, kcat/KM, and KI relative to the native protein. There is no significant change in the regiospecificity of toluene oxidation with any of the T4moD variants
additional information
-
identification of three more toluene monooxygenase-encoding operons. Data suggest the important role of plasmids in the spread of toluene degradative capacity
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Schwartz, J.K.; Wei, P.P.; Mitchell, K.H.; Fox, B.G.; Solomon, E.I.
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7098-7109
2008
Pseudomonas mendocina (Q00456 and Q00457 and Q00460), Pseudomonas mendocina KR1 (Q00456 and Q00457 and Q00460)
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Protein engineering of toluene monooxygenases for synthesis of chiral sulfoxides
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1555-1566
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35
9106-9119
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Pseudomonas mendocina (Q00456 and Q00457 and Q00460 and Q00458 and Q00459), Pseudomonas mendocina, Pseudomonas mendocina KR1 (Q00456 and Q00457 and Q00460 and Q00458 and Q00459)
brenda
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Pseudomonas mendocina (Q00456), Pseudomonas mendocina KR1 (Q00456), Pseudomonas mendocina KR1
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Biochemistry
38
727-739
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Pseudomonas mendocina (Q00458), Pseudomonas mendocina KR1 (Q00458), Pseudomonas mendocina KR1
brenda
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Threonine 201 in the diiron enzyme toluene 4-monooxygenase is not required for catalysis
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39
791-799
2000
Pseudomonas mendocina (Q00456), Pseudomonas mendocina KR1 (Q00456), Pseudomonas mendocina KR1
brenda
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Solution structure of the toluene 4-monooxygenase effector protein (T4moD)
Biochemistry
40
3512-3524
2001
Pseudomonas mendocina (Q00459), Pseudomonas mendocina, Pseudomonas mendocina KR1 (Q00459)
brenda
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Remarkable aliphatic hydroxylation by the diiron enzyme toluene 4-monooxygenase in reactions with radical or cation diagnostic probes norcarane, 1,1-dimethylcyclopropane, and 1,1-diethylcyclopropane
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43
15688-15701
2004
Pseudomonas mendocina (Q00456 and Q00457 and Q00460), Pseudomonas mendocina KR1 (Q00456 and Q00457 and Q00460)
brenda
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Biochemistry
44
7131-7142
2005
Pseudomonas mendocina (Q00459)
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5478-5485
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Pseudomonas mendocina (Q00456 and Q00457 and Q00460 and Q00458 and Q00459), Pseudomonas mendocina KR1 (Q00456 and Q00457 and Q00460 and Q00458 and Q00459)
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48
3838-3846
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Pseudomonas mendocina (Q00456), Pseudomonas mendocina KR1 (Q00456)
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51
1101-1113
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Pseudomonas mendocina (Q00456 and Q00457 and Q00460 and Q00459), Pseudomonas mendocina KR1 (Q00456 and Q00457 and Q00460 and Q00459)
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66
72-80
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-
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57
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5
5009
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Optimized expression and purification of toluene 4-monooxygenase hydroxylase
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20
58-65
2000
Pseudomonas mendocina (Q00456 and Q00457 and Q00460), Pseudomonas mendocina KR1 (Q00456 and Q00457 and Q00460)
brenda