A cytochrome P-450 (heme-thiolate) protein. The enzyme, found in insects except for Lepidoptera (moths and butterflies) is specific for methyl farnesoate (cf. EC 1.14.14.128, farnesoate epoxidase) [1,2].
A cytochrome P-450 (heme-thiolate) protein. The enzyme, found in insects except for Lepidoptera (moths and butterflies) is specific for methyl farnesoate (cf. EC 1.14.14.128, farnesoate epoxidase) [1,2].
the enzyme is specific for methyl (2E,6E)-farnesoate i.e. (2E,6E)-3,7,11-trimethyl-2,6-dodecatrienoate. The enzyme produces juvenile hormone III with a ratio of 98:2 in favor of the natural (10R)-epoxide enantiomer. No activity with methyl (2Z,6Z)-farnesoate, methyl (2Z,6E)-farnesoate
no activity with farnesol, farnesal, farnesoic acid, juvenile hormone III, 3,7,11-trimethyl-dodecanol, geranyl geraniol, farnesyl methyl ether, and geraniol and the fatty acids, lauric acid, and arachidonic acid
no activity with farnesol, farnesal, farnesoic acid, juvenile hormone III, 3,7,11-trimethyl-dodecanol, geranyl geraniol, farnesyl methyl ether, and geraniol and the fatty acids, lauric acid, and arachidonic acid
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
bifunctional compound, inhibits juvenile hormone III synthesis by intact glands as well as methyl farnesoate epoxidation by gland homogenates. Using the compound, a radiolabeled and photoactivatable diazirine or benzophenone group can be introduced to label the hydrophobic substrate binding site of the enzyme
inhibits juvenile hormone biosynthesis in vitro by spontaneously active glands, presumably by direct inhibition of the epoxidase, and also inhibits the production of total methyl ester
bifunctional compound, inhibits juvenile hormone III synthesis by intact glands as well as methyl farnesoate epoxidation by gland homogenates. Using the compound, a radiolabeled and photoactivatable diazirine or benzophenone group can be introduced to label the hydrophobic substrate binding site of the enzyme
CYP15A1 transcripts are detected only in the corpora allata of day 5-mated females. Highest CYP15A1 transcript levels are observed on days 36, at the time of maximal biosynthetic activity of the glands, whereas the message is barely detectable on days 10, 15, and 20, after oviposition and during pregnancy, when juvenile hormone synthesis is low
clear relationship between methyl farnesoate levels and the total rate of juvenile hormone biosynthesis in individual pairs of glands. Methyl farnesoate levels rise 30fold during the spontaneous activity cycle, but never reach a level causing saturation of the methyl farnesoate epoxidase
temporal and spatial expression patterns of gene EcMFE, relative expression profiles of EcMFE in tissues and developmental stages, overview. The expression level of EcMFE is high in the first instar larval stage. Transcripts of EcMFE, EcJHEH and EcJHAMT show a similar tendency with the cantharidin production in male blister beetles after mating
temporal and spatial expression patterns of gene EcMFE, relative expression profiles of EcMFE in tissues and developmental stages, overview. The expression level of EcMFE is high in the first instar larval stage. Transcripts of EcMFE, EcJHEH and EcJHAMT show a similar tendency with the cantharidin production in male blister beetles after mating
gene mfe expression and juvenile hormone, JH, titers in the life cycle of the highly eusocial stingless bee, hemolymph JH titers for all life cycle stages of Melipona scutellaris queens and workers, overview. The JH titer is high in the second larval instar, drops in the third, and rises again as the larvae enter metamorphosis. During the pupal stage, mfe expression is initialy elevated, but then gradually drops to low levels before adult emergence. No variation is seen in the JH titer. In adult virgin queens, mfe expression and the JH titer are significantly elevated, possibly associated with their reproductive potential. For workers, JH titers are lower in foragers than in nurse bees, while mfe expression does not differ
gene mfe expression and juvenile hormone, JH, titers in the life cycle of the highly eusocial stingless bee, hemolymph JH titers for all life cycle stages of Melipona scutellaris queens and workers, overview. The JH titer is high in the second larval instar, drops in the third, and rises again as the larvae enter metamorphosis. During the pupal stage, mfe expression is initialy elevated, but then gradually drops to low levels before adult emergence. No variation is seen in the JH titer. In adult virgin queens, mfe expression and the JH titer are significantly elevated, possibly associated with their reproductive potential. For workers, JH titers are lower in foragers than in nurse bees, while mfe expression does not differ
level of CYP15A1 mRNA is high in the embryonic stage as well as in the middle of the final larval instar. In the embryonic stage, the transcript level of CYP15A1 starts to increase 30 h after egg laying and peaks 54-60 h after egg laying
level of CYP15A1 mRNA is high in the embryonic stage as well as in the middle of the final larval instar. In the embryonic stage, the transcript level of CYP15A1 starts to increase 30 h after egg laying and peaks 54-60 h after egg laying
BgInR knockdown leads to a severe inhibition of juvenile hormone synthesis in adult female corpora allata, with a concomitant reduction of mRNA levels corresponding to 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase-1, HMG-CoA synthase-2, HMG-CoA reductase and methyl farnesoate epoxidase, phenotype, overview
juvenile hormone is finally synthesized via methylation and epoxidation regulated by JH acid O-methyltransferase (JHAMT) and methyl farnesoate epoxidase (MFE), respectively. Genes EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but juvenile hormone III (JH III) might not be the direct precursor of cantharidin. Cantharidin is a monoterpene that is proposed to be constituted of two isoprene units. Cantharidin is biosynthesized via the mevalonate pathway that can also synthesize JH III
RNAi-mediated knockdown of CYP15A1 in the pre-final larval instar does not result in precocious metamorphosis to pupa. The double knockdown of juvenile hormone acid O-methyltransferase and TcCYP15A1 results in pupae and adults with shorter wings
the methyl farnesoate epoxidase gene (MFE) is an indicator of juvenile hormone production. Virgin queens are distinguished by higher expression of forkhead box protein O and downregulated insulin-like peptides and juvenile hormone signaling, indicated by low expression of methyl farnesoate epoxidase (MFE) and transcription factor Krüppel homolog 1 (Kr-h1), while reproducing queens reveal an upregulation of MFE and vitellogenin together with insulin signaling. Workers exhibit an expression pattern of MFE and vitellogenin similar to that of reproducing queens. Males aere characterized by high Kr-h1expression and low vitellogenin level
EcMFE RNAi-mediated knockdown. In EcMFE dsRNA treatment, there are no significant differences between the treated group and controls until the 5th day after injection, when compared on cantharidin content, the cantharidin content is significantly reduced on the 7th day after injection
EcMFE RNAi-mediated knockdown. In EcMFE dsRNA treatment, there are no significant differences between the treated group and controls until the 5th day after injection, when compared on cantharidin content, the cantharidin content is significantly reduced on the 7th day after injection
Methyl farnesoate epoxidase (mfe) gene expression and juvenile hormone titers in the life cycle of a highly eusocial stingless bee, Melipona scutellaris