Information on EC 1.14.14.49 - 12-hydroxyjasmonoyl-L-amino acid 12-hydroxylase

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The expected taxonomic range for this enzyme is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
1.14.14.49
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RECOMMENDED NAME
GeneOntology No.
12-hydroxyjasmonoyl-L-amino acid 12-hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 12-hydroxyjasmonoyl-L-amino acid + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 = a 12-hydroxy-12-oxojasmonoyl-L-amino acid + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
show the reaction diagram
overall reaction
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a 12-hydroxyjasmonoyl-L-amino acid + [reduced NADPH-hemoprotein reductase] + O2 = a 12-oxojasmonoyl-L-amino acid + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
(1a)
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a 12-oxojasmonoyl-L-amino acid + [reduced NADPH-hemoprotein reductase] + O2 = a 12-hydroxy-12-oxojasmonoyl-L-amino acid + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
(1b)
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SYSTEMATIC NAME
IUBMB Comments
12-hydroxyjasmonoyl-L-amino acid,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (12-hydroxylating)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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the enzyme plays an essential role for jasmonate response after wounding
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
12-hydroxy-dodecanoic acid + reduced NADPH-hemoprotein reductase + O2
12-oxo-dodecanoic acid + oxidized NADPH-hemoprotein reductase + H2O
show the reaction diagram
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-
-
?
12-hydroxyjasmonoyl-L-isoleucine + reduced NADPH-hemoprotein reductase + O2
12-hydroxy-12-oxojasmonoyl-L-isoleucine + oxidized NADPH-hemoprotein reductase + H2O
show the reaction diagram
-
-
-
?
12-hydroxyjasmonoyl-L-isoleucine + [reduced NADPH-hemoprotein reductase] + O2
12-hydroxy-12-oxojasmonoyl-L-isoleucine + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
12-hydroxylauric acid + reduced NADPH-hemoprotein reductase + O2
12-hydroxy-dodecanoic acid + oxidized NADPH-hemoprotein reductase + H2O
show the reaction diagram
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-
-
?
12-oxo-dodecanoic acid + reduced NADPH-hemoprotein reductase + O2
dodecadioic acid + oxidized NADPH-hemoprotein reductase + H2O
show the reaction diagram
-
-
-
?
jasmonoyl-L-isoleucine + [reduced NADPH-hemoprotein reductase] + O2
12-hydroxyjasmonoyl-L-isoleucine + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
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-
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
12-hydroxyjasmonoyl-L-isoleucine + [reduced NADPH-hemoprotein reductase] + O2
12-hydroxy-12-oxojasmonoyl-L-isoleucine + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
-
?
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.1
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calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 56566, calculated from amino acid sequence
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strain WAT11
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expressed in Saccharomyces cerevisiae WAT11 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is induced by methyl jasmonate and wounding
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gene expression is clearly upregulated after fungal infection with Botrytis cinerea