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3-phospho-D-glyceroyl phosphate + NADH + H+
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Substrates: -
Products: -
r
3-phospho-D-glyceroyl phosphate + NADPH + H+
D-glyceraldehyde 3-phosphate + phosphate + NADP+
D-glyceraldehyde 3-phosphate + NADP+ + H2O
3-phospho-D-glycerate + NADPH + H+
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+
3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
additional information
?
-
3-phospho-D-glyceroyl phosphate + NADPH + H+
D-glyceraldehyde 3-phosphate + phosphate + NADP+
Substrates: in the gluconeogenic direction the enzyme is specific for NADPH
Products: -
r
3-phospho-D-glyceroyl phosphate + NADPH + H+
D-glyceraldehyde 3-phosphate + phosphate + NADP+
Substrates: in the gluconeogenic direction the enzyme is specific for NADPH
Products: -
r
D-glyceraldehyde 3-phosphate + NADP+ + H2O
3-phospho-D-glycerate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
3-phospho-D-glycerate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+
3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+
3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+
3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
-
Substrates: -
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
-
Substrates: -
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
-
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
-
Substrates: -
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
-
Substrates: -
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: both NAD+ and NADP+ are utilized in the glycolytic direction
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: comparable activity with NADP+ and NAD+
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: both NAD+ and NADP+ are utilized in the glycolytic direction
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: comparable activity with NADP+ and NAD+
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
-
Substrates: -
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
-
Substrates: -
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
-
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: NADP+ is the preferred cofactor
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: both NAD+ and NADP+ are utilized in the glycolytic direction
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: comparable activity with NADP+ and NAD+
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: both NAD+ and NADP+ are utilized in the glycolytic direction
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: comparable activity with NADP+ and NAD+
Products: -
r
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: r
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
1,3-diphosphoglycerate + NAD(P)H
-
Substrates: r
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: r
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: -
Products: -
?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
1,3-diphosphoglycerate + NADPH
-
Substrates: r
Products: -
?
additional information
?
-
Substrates: the enzyme plays dual coenzyme specificity with NAD+ and NADP+
Products: -
-
additional information
?
-
Substrates: glyceraldehyde-3-phosphate dehydrogenase from chloroplasts has a dual cofactor specificity and can use both NADPH and NADH
Products: -
-
additional information
?
-
-
Substrates: glyceraldehyde-3-phosphate dehydrogenase from chloroplasts has a dual cofactor specificity and can use both NADPH and NADH
Products: -
-
additional information
?
-
Substrates: highest activity in Bis-Tris buffer, lowest in imidazole-HCL, overview
Products: -
-
additional information
?
-
-
Substrates: highest activity in Bis-Tris buffer, lowest in imidazole-HCL, overview
Products: -
-
additional information
?
-
Substrates: highest activity in Bis-Tris buffer, lowest in imidazole-HCL, overview
Products: -
-
additional information
?
-
Substrates: highest activity in Bis-Tris buffer, lowest in imidazole-HCL, overview
Products: -
-
additional information
?
-
-
Substrates: high hydrolase activity with a variety of organic p-nitrophenyl esters
Products: -
?
additional information
?
-
-
Substrates: possible amphibolic role: anabolic in photosynthetic carbon assimilation and catabolic in carbohydrate degradative pathways
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3-phospho-D-glyceroyl phosphate + NADPH + H+
D-glyceraldehyde 3-phosphate + phosphate + NADP+
D-glyceraldehyde 3-phosphate + NADP+ + H2O
3-phospho-D-glycerate + NADPH + H+
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+
3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
additional information
?
-
-
Substrates: possible amphibolic role: anabolic in photosynthetic carbon assimilation and catabolic in carbohydrate degradative pathways
Products: -
?
3-phospho-D-glyceroyl phosphate + NADPH + H+
D-glyceraldehyde 3-phosphate + phosphate + NADP+
Substrates: in the gluconeogenic direction the enzyme is specific for NADPH
Products: -
r
3-phospho-D-glyceroyl phosphate + NADPH + H+
D-glyceraldehyde 3-phosphate + phosphate + NADP+
Substrates: in the gluconeogenic direction the enzyme is specific for NADPH
Products: -
r
D-glyceraldehyde 3-phosphate + NADP+ + H2O
3-phospho-D-glycerate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
3-phospho-D-glycerate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+
3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+
3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+
3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
-
Substrates: -
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
-
Substrates: -
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
-
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: both NAD+ and NADP+ are utilized in the glycolytic direction
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
Substrates: both NAD+ and NADP+ are utilized in the glycolytic direction
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
-
Substrates: -
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
-
Substrates: -
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
-
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: -
Products: -
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: both NAD+ and NADP+ are utilized in the glycolytic direction
Products: -
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
Substrates: both NAD+ and NADP+ are utilized in the glycolytic direction
Products: -
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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D35G
site-directed mutagenesis, the mutant enzyme accepts both NAD+ and NADP+ , the catalytic efficiency with NADP+ is 3fold lower than with NAD+
D35G/L36R/P192S
site-directed mutagenesis, the mutant enzyme accepts both NAD+ and NADP+ with similar catalytic efficiency
D35G/L36T/T37K
site-directed mutagenesis, introducing a third mutation T37K into the mutant D35G/L36T completely reverses the coenzyme specificity of the enzyme
D35G/L36T/T37K/P192S
site-directed mutagenesis, the mutant shows high catalytic efficiency with NADP+ while the catalytic efficiency with NAD+ also increases. The replacement of Pro192 to Ser benefits the binding affinity of both NAD+ and NADP+
L36T
site-directed mutagenesis, the mutant enzyme accepts both NAD+ and NADP+ , the catalytic efficiency with NADP+ is lower than with NAD+
D35G
-
site-directed mutagenesis, the mutant enzyme accepts both NAD+ and NADP+ , the catalytic efficiency with NADP+ is 3fold lower than with NAD+
-
D35G/L36R/P192S
-
site-directed mutagenesis, the mutant enzyme accepts both NAD+ and NADP+ with similar catalytic efficiency
-
D35G/L36T/T37K
-
site-directed mutagenesis, introducing a third mutation T37K into the mutant D35G/L36T completely reverses the coenzyme specificity of the enzyme
-
L36T
-
site-directed mutagenesis, the mutant enzyme accepts both NAD+ and NADP+ , the catalytic efficiency with NADP+ is lower than with NAD+
-
D35G
-
site-directed mutagenesis, the mutant enzyme accepts both NAD+ and NADP+ , the catalytic efficiency with NADP+ is 3fold lower than with NAD+
-
D35G/L36R/P192S
-
site-directed mutagenesis, the mutant enzyme accepts both NAD+ and NADP+ with similar catalytic efficiency
-
D35G/L36T/T37K
-
site-directed mutagenesis, introducing a third mutation T37K into the mutant D35G/L36T completely reverses the coenzyme specificity of the enzyme
-
L36T
-
site-directed mutagenesis, the mutant enzyme accepts both NAD+ and NADP+ , the catalytic efficiency with NADP+ is lower than with NAD+
-
C150S
-
the mutant is unable to turn over D-glyceraldehyde 3-phosphate in the presence of either NAD+ or NADP+
C150S
-
the mutant is unable to turn over D-glyceraldehyde 3-phosphate in the presence of either NAD+ or NADP+
-
additional information
the coenzyme specificity of GAPDH, EC 1.2.1.12, of Corynebacterium glutamicum is systematically manipulated by rational protein design and the effect of the manipulation for cellular metabolism and lysine production is evaluated. By a combinatorial modification of four key residues within the coenzyme binding sites, different GAPDH mutants with varied coenzyme specificity are constructed. While increasing the catalytic efficiency of GAPDH towards NADP+ enhances lysine production in all of the tested mutants, the most significant improvement of lysine production (about 60%) is achieved with the mutant showing similar preference towards both NAD+ and NADP+, EC 1.2.1.59
additional information
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the coenzyme specificity of GAPDH, EC 1.2.1.12, of Corynebacterium glutamicum is systematically manipulated by rational protein design and the effect of the manipulation for cellular metabolism and lysine production is evaluated. By a combinatorial modification of four key residues within the coenzyme binding sites, different GAPDH mutants with varied coenzyme specificity are constructed. While increasing the catalytic efficiency of GAPDH towards NADP+ enhances lysine production in all of the tested mutants, the most significant improvement of lysine production (about 60%) is achieved with the mutant showing similar preference towards both NAD+ and NADP+, EC 1.2.1.59
additional information
-
the coenzyme specificity of GAPDH, EC 1.2.1.12, of Corynebacterium glutamicum is systematically manipulated by rational protein design and the effect of the manipulation for cellular metabolism and lysine production is evaluated. By a combinatorial modification of four key residues within the coenzyme binding sites, different GAPDH mutants with varied coenzyme specificity are constructed. While increasing the catalytic efficiency of GAPDH towards NADP+ enhances lysine production in all of the tested mutants, the most significant improvement of lysine production (about 60%) is achieved with the mutant showing similar preference towards both NAD+ and NADP+, EC 1.2.1.59
-
additional information
-
the coenzyme specificity of GAPDH, EC 1.2.1.12, of Corynebacterium glutamicum is systematically manipulated by rational protein design and the effect of the manipulation for cellular metabolism and lysine production is evaluated. By a combinatorial modification of four key residues within the coenzyme binding sites, different GAPDH mutants with varied coenzyme specificity are constructed. While increasing the catalytic efficiency of GAPDH towards NADP+ enhances lysine production in all of the tested mutants, the most significant improvement of lysine production (about 60%) is achieved with the mutant showing similar preference towards both NAD+ and NADP+, EC 1.2.1.59
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