Information on EC 1.2.99.10 - 4,4'-diapolycopenoate synthase

for references in articles please use BRENDA:EC1.2.99.10
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.99.10
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RECOMMENDED NAME
GeneOntology No.
4,4'-diapolycopenoate synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4,4'-diapolycopen-4-al + H2O + acceptor = 4,4'-diapolycopen-4-oate + reduced acceptor
show the reaction diagram
(1)
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4,4'-diapolycopene-4,4'-dial + 2 H2O + 2 acceptor = 4,4'-diapolycopene-4,4'-dioate + 2 reduced acceptor
show the reaction diagram
(2)
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4,4'-diapolycopenedioate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
4,4'-diapolycopen-4-al,donor:oxygen oxidoreductase (4,4'-diapolycopen-4-oate-forming)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
coexpression of the crtN ald crtNb gene cluster from Methylomonas sp. with Staphylococcus aureus crtM in Escherichia coli results in synthesis of 4,4'-diaponeurosporene-4,4'-dioic acid as a pink pigment
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4,4'-diapolycopen-4-al + H2O + acceptor
4,4'-diapolycopen-4-oate + reduced acceptor
show the reaction diagram
4,4'-diapolycopene + H2O + acceptor
4,4'-diapolycopene-4,4'-dial + reduced acceptor
show the reaction diagram
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?
4,4'-diapolycopene-4,4'-dial + H2O + acceptor
4,4'-diapolycopene-4,4'-dioate + reduced acceptor
show the reaction diagram
4,4'-diaponeurosporen-4,4'-dial + acceptor
4,4'-diaponeurosporene-4,4'-dioic acid + reduced acceptor
show the reaction diagram
4,4'-diaponeurosporen-4,4'-dial is a dark-reddish purple pigment
product is a pink pigment with absorption maxima at 465, 490, and 519 nm
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?
4,4'-diaponeurosporene + H2O + acceptor
4,4'-diaponeurosporen-4-al + reduced acceptor
show the reaction diagram
high activity
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?
additional information
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the enzyme has moderate to low activity on neurosporene and lycopene and no activity on beta-carotene or zeta-carotene
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4,4'-diapolycopen-4-al + H2O + acceptor
4,4'-diapolycopen-4-oate + reduced acceptor
show the reaction diagram
4,4'-diapolycopene-4,4'-dial + H2O + acceptor
4,4'-diapolycopene-4,4'-dioate + reduced acceptor
show the reaction diagram
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells