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(3,3-difluorobutyryl)pantetheine + phenazine methosulfate + dichlorophenolindophenol
3-fluoro-2-butenoylpantetheine + HF
-
-
-
?
(E)-2-butenoyl-CoA + reduced methylviologen
? + oxidized methylviologen
-
-
-
-
?
(S)-2-methylbutyryl-CoA + phenazine methosulfate + dichloroindophenol
(S)-2-methyl-2-butenoyl-CoA + reduced acceptor
-
6.0% of activity with butyryl-CoA
-
?
2-azabutyryl-CoA + ?
?
-
substrate may be converted by intrinsic hydratase activity of the enzyme to 2-azaacetyl-CoA
-
-
?
2-butenoyl-CoA + reduced acceptor
butanoyl-CoA + acceptor
-
reduction in vivo
-
-
r
2-butenoyl-CoA + reduced acceptor
butyryl-CoA + acceptor
-
trans-addition of hydrogen
-
?
2-butenoyl-CoA + reduced electron transfer flavoprotein
butanoyl-CoA + electron transfer protein
-
enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro
-
r
2-hexenoyl-CoA + reduced electron transfer flavoprotein
hexanoyl-CoA + electron transfer protein
-
enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro
-
r
2-methylbutanoyl-CoA + acceptor
2-methyl-2-butenoyl-CoA + reduced acceptor
-
-
-
-
r
2-methylbutyryl-CoA + electron acceptor
2-methyl-2-butenoyl-CoA + reduced acceptor
-
highest activity
-
-
?
2-methylpropionyl-CoA + phenazine methosulfate + dichloroindophenol
2-methylpropenoyl-CoA + reduced acceptor
-
1.5% of activity with butyryl-CoA
-
?
2-pentenoyl-CoA + reduced electron transfer flavoprotein
pentanoyl-CoA + electron transfer protein
-
enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro
-
r
2-propenoyl-CoA + reduced electron transfer flavoprotein
propanoyl-CoA + electron transfer protein
3-fluoropropionyl-CoA + phenazine methosulfate + dichlorophenolindophenol
2-propenoyl-CoA + HF
-
-
-
?
3-hydroxybutyryl-CoA + acceptor
acetoacetyl-CoA + reduced acceptor
4-methylpentanoyl-CoA + phenazine methosulfate + dichloroindophenol
4-methyl-2-pentenoyl-CoA + reduced acceptor
-
18.0% of activity with butyryl-CoA
-
?
acrolein + reduced methylviologen
? + oxidized methylviologen
-
-
-
-
?
acryloyl-CoA + benzyl viologen
propanoyl-CoA + reduced benzyl viologen
acryloyl-CoA + reduced methylviologen
propanoyl-CoA + oxidized methylviologen
-
-
-
-
?
butanoyl-CoA + acceptor
2-butenoyl-CoA + reduced acceptor
butanoyl-CoA + acceptor
but-2-enoyl-CoA + reduced acceptor
butanoyl-CoA + FAD
2-butenoyl-CoA + FADH2
butanoyl-CoA + FAD
but-2-enoyl-CoA + FADH2
-
-
-
?
butanoyl-CoA + FAD
trans-2,3-dehydrobutanoyl-CoA + FADH2
-
-
-
-
?
butanoyl-CoA + oxidized acceptor
crotonyl-CoA + reduced acceptor
-
-
-
-
r
butyryl-CoA + 2,6-dichloro-phenolindophenol
but-2-enoyl-CoA + reduced 2,6-dichloro-phenolindophenol
-
-
-
-
r
butyryl-CoA + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
2-butenoyl-CoA + reduced acceptor
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
butyryl-CoA + electron transfer flavoprotein
2-butenoyl-CoA + reduced electron transfer flavoprotein
-
-
-
-
?
butyryl-CoA + electron transfer flavoprotein
crotonyl-CoA + reduced electron transfer flavoprotein
-
-
-
-
?
butyryl-CoA + electron-transferring flavoprotein
2-butenoyl-CoA + reduced electron-transferring flavoprotein
-
-
-
-
?
butyryl-CoA + ferricenium
2-butenoyl-CoA + ferrocene
-
-
-
-
?
butyryl-CoA + ferricytochrome c
butenoyl-CoA + reduced acceptor
-
-
-
r
butyryl-CoA + ferrocenium
2-butenoyl-CoA + ferrocene
-
-
-
-
?
butyryl-CoA + O2
2-butenoyl-CoA + H2O2
butyryl-CoA + oxidized acceptor
crotonyl-CoA + reduced acceptor
-
-
-
-
?
butyryl-CoA + pyocyanine + triphenyltetrazolium chloride
2-butenoyl-CoA + reduced acceptor
-
-
-
r
crotonyl-CoA + 2 NADH + 2 Fd-
butyryl-CoA + 2 NAD+ + 2 Fd2-
crotonyl-CoA + electron acceptor
butyryl-CoA + reduced acceptor
-
-
-
-
?
crotonyl-CoA + electron transfer flavoprotein
butyryl-CoA + ?
-
-
-
?
crotonyl-CoA + O2 + 2 NADH + 2 H+
butanoyl-CoA + H2O2 + 2 NAD+
crotonyl-CoA + reduced acceptor
butyryl-CoA + oxidized acceptor
-
-
-
-
?
crotonyl-CoA + reduced ferredoxin
butanoyl-CoA + oxidized ferredoxin
cyclobutanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol
cyclo-2-butenecarboxyl-CoA + reduced acceptor
cycloheptanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol
cyclo-2-heptenecarboxyl-CoA + reduced acceptor
-
3.2% of activity with butyryl-CoA
-
?
cyclohexanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol
cyclo-2-hexenecarboxyl-CoA + reduced acceptor
-
6.7% of activity with butyryl-CoA
-
?
cyclopentanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol
cyclo-2-pentenecarboxyl-CoA + reduced acceptor
ethyl vinyl ketone + reduced methylviologen
? + oxidized methylviologen
-
-
-
-
?
heptanoyl-CoA + electron acceptor
2-heptenoyl-CoA + reduced acceptor
hexanoyl-CoA + acceptor
2-hexenoyl-CoA + reduced acceptor
-
-
-
-
r
hexanoyl-CoA + acceptor
hex-2-enoyl-CoA + reduced acceptor
-
-
-
?
hexanoyl-CoA + electron acceptor
2-hexenoyl-CoA + reduced acceptor
hexanoyl-CoA + electron transfer flavoprotein
2-hexenoyl-CoA + reduced acceptor
hexanoyl-CoA + FAD
hex-2-enoyl-CoA + FADH2
-
-
-
?
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
2-hexenoyl-CoA + reduced acceptor
N-acetyl-S-acryloyl-cysteamine + reduced methylviologen
? + oxidized methylviologen
-
-
-
-
?
octanoyl-CoA + electron acceptor
2-octenoyl-CoA + reduced acceptor
octanoyl-CoA + electron transfer flavoprotein
octenoyl-CoA + reduced acceptor
-
-
-
-
?
octanoyl-CoA + electron transfer protein
2-octenoyl-CoA + reduced acceptor
-
-
-
?
octanoyl-CoA + Meldola's Blue + iodonitrotetrazolium chloride
2-octenoyl-CoA + reduced acceptor
pentanoyl-CoA + 2,6-dichlorophenolindophenol + phenazine ethosulfate
2-pentenoyl-CoA + reduced acceptor
pentanoyl-CoA + acceptor
pent-2-enoyl-CoA + reduced acceptor
-
-
-
?
pentanoyl-CoA + electron transfer flavoprotein
2-pentenoyl-CoA + reduced acceptor
-
-
-
?
pentanoyl-CoA + FAD
pent-2-enoyl-CoA + FADH2
-
-
-
?
pentanoyl-CoA + phenazine methosulfate
2-pentenoyl-CoA + reduced acceptor
pentenoyl-CoA + oxidized electron transfer flavoprotein
valeryl-CoA + reduced electron transfer flavoprotein
-
-
-
-
?
propionyl-CoA + 2,6-dichlorophenolindophenol
2-propenoyl-CoA + reduced acceptor
propionyl-CoA + electron transfer flavoprotein
2-propenoyl-CoA + reduced acceptor
-
-
-
?
propionyl-CoA + phenazine methosulfate
2-propenoyl-CoA + reduced acceptor
-
-
-
?
reduced ferredoxin + NAD+ + H+
oxidized ferredoxin + NADH
S-butyrylpantetheine + phenazine ethosulfate + dichloroindophenol
S-but-2-eneoylpantetheine + reduced acceptor
-
51.0% of activity with butyryl-CoA
-
?
valeryl-CoA + electron acceptor
2-pentenoyl-CoA + reduced acceptor
-
-
-
-
?
additional information
?
-
2-propenoyl-CoA + reduced electron transfer flavoprotein

propanoyl-CoA + electron transfer protein
-
-
-
-
?
2-propenoyl-CoA + reduced electron transfer flavoprotein
propanoyl-CoA + electron transfer protein
-
enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro
-
r
3-hydroxybutyryl-CoA + acceptor

acetoacetyl-CoA + reduced acceptor
-
-
-
?
3-hydroxybutyryl-CoA + acceptor
acetoacetyl-CoA + reduced acceptor
-
-
-
?
acryloyl-CoA + benzyl viologen

propanoyl-CoA + reduced benzyl viologen
-
-
-
-
?
acryloyl-CoA + benzyl viologen
propanoyl-CoA + reduced benzyl viologen
-
-
-
-
?
butanoyl-CoA + acceptor

2-butenoyl-CoA + reduced acceptor
-
-
-
-
r
butanoyl-CoA + acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
-
r
butanoyl-CoA + acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
-
r
butanoyl-CoA + acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
r
butanoyl-CoA + acceptor

but-2-enoyl-CoA + reduced acceptor
-
-
-
-
?
butanoyl-CoA + acceptor
but-2-enoyl-CoA + reduced acceptor
-
-
-
?
butanoyl-CoA + acceptor
but-2-enoyl-CoA + reduced acceptor
-
-
-
-
?
butanoyl-CoA + FAD

2-butenoyl-CoA + FADH2
-
-
-
?
butanoyl-CoA + FAD
2-butenoyl-CoA + FADH2
-
-
-
-
?
butyryl-CoA + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

2-butenoyl-CoA + reduced acceptor
-
-
-
?
butyryl-CoA + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
2-butenoyl-CoA + reduced acceptor
-
-
-
?
butyryl-CoA + electron acceptor

2-butenoyl-CoA + reduced acceptor
-
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
r
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptors phenazine methosulfate or phenazine ethosulfate
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptors phenazine methosulfate or Meldola' s Blue (i.e. 8-dimethylamino-2,3-benzophenoxazinium chloride)
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor 2,6-dichlorophenol-indophenol
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor 2,6-dichlorophenol-indophenol
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor iodonitrotetrazolium chloride
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptor phenazine methosulfate
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor 2,6-dichlorophenol-indophenol
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor 2,6-dichlorophenol-indophenol
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptors phenazine methosulfate or electron transfer flavoprotein
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor 2,6-dichlorophenol-indophenol
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptor phenazine methosulfate
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
r
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptors phenazine methosulfate or phenazine ethosulfate
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptors phenazine methosulfate or Meldola' s Blue (i.e. 8-dimethylamino-2,3-benzophenoxazinium chloride)
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor 2,6-dichlorophenol-indophenol
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor 2,6-dichlorophenol-indophenol
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
enzyme may also have an intrinsic crotonase activity
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor iodonitrotetrazolium chloride
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor iodonitrotetrazolium chloride
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptor phenazine methosulfate
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptor phenazine methosulfate
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptors phenazine methosulfate or electron transfer flavoprotein
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptors phenazine methosulfate or electron transfer flavoprotein
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
no activity towards dicarboxylic-CoAs
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor 2,6-dichlorophenol-indophenol
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor 2,6-dichlorophenol-indophenol
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor 2,6-dichlorophenol-indophenol
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
terminal electron acceptor 2,6-dichlorophenol-indophenol
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptor phenazine methosulfate
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptor phenazine methosulfate
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptor phenazine methosulfate
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
intermediate electron acceptor phenazine methosulfate
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
?
butyryl-CoA + electron acceptor
2-butenoyl-CoA + reduced acceptor
-
-
-
?
butyryl-CoA + O2

2-butenoyl-CoA + H2O2
-
-
-
?
butyryl-CoA + O2
2-butenoyl-CoA + H2O2
-
-
-
?
crotonyl-CoA + 2 NADH + 2 Fd-

butyryl-CoA + 2 NAD+ + 2 Fd2-
-
-
-
?
crotonyl-CoA + 2 NADH + 2 Fd-
butyryl-CoA + 2 NAD+ + 2 Fd2-
-
-
-
?
crotonyl-CoA + O2 + 2 NADH + 2 H+

butanoyl-CoA + H2O2 + 2 NAD+
the crotonyl-CoA reduction mediated by the Clostridium difficile enzyme appears to be decoupled from ferredoxin reduction in the presence of air. Here, molecular oxygen apparently serves as an electron acceptor and hydrogen peroxide is formed as a second product
-
-
?
crotonyl-CoA + O2 + 2 NADH + 2 H+
butanoyl-CoA + H2O2 + 2 NAD+
-
the crotonyl-CoA reduction mediated by the Clostridium difficile enzyme appears to be decoupled from ferredoxin reduction in the presence of air. Here, molecular oxygen apparently serves as an electron acceptor and hydrogen peroxide is formed as a second product
-
-
?
crotonyl-CoA + reduced ferredoxin

butanoyl-CoA + oxidized ferredoxin
-
-
-
?
crotonyl-CoA + reduced ferredoxin
butanoyl-CoA + oxidized ferredoxin
-
-
-
?
crotonyl-CoA + reduced ferredoxin
butanoyl-CoA + oxidized ferredoxin
-
-
-
?
crotonyl-CoA + reduced ferredoxin
butanoyl-CoA + oxidized ferredoxin
-
-
-
-
?
cyclobutanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol

cyclo-2-butenecarboxyl-CoA + reduced acceptor
-
-
-
-
?
cyclobutanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol
cyclo-2-butenecarboxyl-CoA + reduced acceptor
-
1.0% of activity with butyryl-CoA
-
?
cyclopentanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol

cyclo-2-pentenecarboxyl-CoA + reduced acceptor
-
-
-
-
?
cyclopentanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol
cyclo-2-pentenecarboxyl-CoA + reduced acceptor
-
2.1% of activity with butyryl-CoA
-
?
heptanoyl-CoA + electron acceptor

2-heptenoyl-CoA + reduced acceptor
-
-
-
-
?
heptanoyl-CoA + electron acceptor
2-heptenoyl-CoA + reduced acceptor
-
low activity
-
-
?
heptanoyl-CoA + electron acceptor
2-heptenoyl-CoA + reduced acceptor
-
low activity
-
-
?
hexanoyl-CoA + electron acceptor

2-hexenoyl-CoA + reduced acceptor
-
-
-
-
?
hexanoyl-CoA + electron acceptor
2-hexenoyl-CoA + reduced acceptor
-
-
-
-
?
hexanoyl-CoA + electron acceptor
2-hexenoyl-CoA + reduced acceptor
-
-
-
-
?
hexanoyl-CoA + electron transfer flavoprotein

2-hexenoyl-CoA + reduced acceptor
-
-
-
?
hexanoyl-CoA + electron transfer flavoprotein
2-hexenoyl-CoA + reduced acceptor
-
-
-
-
?
hexanoyl-CoA + electron transfer flavoprotein
2-hexenoyl-CoA + reduced acceptor
-
-
-
?
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol

2-hexenoyl-CoA + reduced acceptor
-
-
-
-
?
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
2-hexenoyl-CoA + reduced acceptor
-
-
-
?
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
2-hexenoyl-CoA + reduced acceptor
-
52% of activity with butyryl-CoA
-
?
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
2-hexenoyl-CoA + reduced acceptor
-
40% of activity with butyryl-CoA
-