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The expected taxonomic range for this enzyme is: Nitrobacter winogradskyi
Synonyms
dehydrogenase, glycine (cytochrome), glycine-cytochrome c reductase, reductase, glycine-cytochrome c, more
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dehydrogenase, glycine (cytochrome)
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glycine-cytochrome c reductase
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reductase, glycine-cytochrome c
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glycine + H2O + 2 ferricytochrome c = glyoxylate + NH3 + 2 ferrocytochrome c + 2 H+
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oxidative deamination
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glycine:ferricytochrome-c oxidoreductase (deaminating)
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glycine + ferricytochrome c
glyoxylate + ferrocytochrome c + NH3
glycine + H2O + ferricytochrome c
glyoxylate + NH3 + ferrocytochrome c + H+
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Substrates: -
Products: -
r
glycine + ferricytochrome c
glyoxylate + ferrocytochrome c + NH3
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Substrates: -
Products: -
ir
glycine + ferricytochrome c
glyoxylate + ferrocytochrome c + NH3
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Substrates: highly specific
Products: -
ir
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glycine + ferricytochrome c
glyoxylate + ferrocytochrome c + NH3
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Substrates: -
Products: -
ir
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Cu2+
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55% inhibition at 1 mM
cytochrome c
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above 0.15 mM
diphosphate
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buffer, 10 mM
EDTA
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64% inhibition at 7.1 mM
KCl
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80% inhibition at 75 mM
KCN
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47% inhibition at 7.1 mM
KNO3
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80% inhibition at 75 mM
MgCl2
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80% inhibition at 40 mM
NaCl
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80% inhibition at 75 mM
phosphate
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buffer, 100 mM
pyridoxal 5'-phosphate
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up to 80% inhibition at 0.2-2 mM due to reaction with glycine
pyruvate
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70% inhibition at 10 mM and 20% inhibition at 3 mM
Quinacrine hydrochloride
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0.3-1.0 mM
Sodium azide
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10% inhibition at 2 mM
sodium barbital
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buffer, 100 mM, pH 8.0
Tris
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buffer, 100 mM, pH 8.0
Urea
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30% inhibition at 50 mM
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0.016
ferricytochrome c
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6.1 - 6.9
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in potassium phosphate or diphosphate buffer
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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69000
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gel filtration, sucrose density gradient centrifugation
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4
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4-10 weeks, 30-50% loss of activity
70
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5 min: 75% loss of activity, 2 min: 50% loss of activity
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2 min: 100% loss of activity
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4°C, 4-10 weeks, 30-50% loss of activity
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Sanders, H.K.; Becker, G.E.; Nason, A.
Glycine-cytochrome c reductase from Nitrobacter agilis
J. Biol. Chem.
247
2015-2025
1972
Nitrobacter winogradskyi
brenda
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