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3,4-dehydro-L-proline + acceptor + H2O
? + reduced acceptor
efficient substrate
-
-
?
4-methylene-L-proline + acceptor + H2O
DELTA1-pyrroline-3-methylene-5-carboxylic acid + reduced acceptor
-
-
-
?
glycine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
L-alanine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
L-alanine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
?
L-arginine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
L-histidine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
100% activity compared to L-proline
-
-
?
L-hydroxyproline + oxidized 2,6-dichlorophenolindophenol + H2O
?
-
reaction rate is 39% of that with L-proline
-
-
?
L-leucine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
42% activity compared to L-proline
-
-
?
L-pipecolic acid + acceptor + H2O
? + reduced acceptor
-
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
L-proline + 2,6-dichlorophenolindophenol + H2O
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
-
-
-
?
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
L-proline + acceptor
(S)-1-pyrroline-5-carboxylate + reduced acceptor
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
L-proline + coenzyme Q1 + H2O
DELTA1-pyrroline-5-carboxylate + reduced coenzyme Q1
-
-
-
?
L-proline + cytochrome c + H2O
(S)-1-pyrroline-5-carboxylate + reduced cytochrome c
L-proline + FAD
(S)-1-pyrroline-5-carboxylate + FADH2
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
L-proline + H2O
(S)-1-pyrroline-5-carboxylate + H2O2
-
-
-
?
L-proline + menadione
(S)-1-pyrroline-5-carboxylate + menadiol
-
-
-
-
r
L-proline + oxidized 2,6-dichlorophenolindophenol + H2O
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the enzyme does not utilize ferricyanide or 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide as the electron acceptor, activity with phenazine methosulfate-p-iodonitrotetrazolium violet is 10% of that with 2,6-dichlorophenolindophenol
-
-
?
L-proline + oxidized phenazine methosulfate
(S)-1-pyrroline-5-carboxylate + reduced phenazine methosulfate
-
-
-
-
?
L-proline + p-iodonitrotetrazolium + H2O
(S)-1-pyrroline-5-carboxylate + reduced p-iodonitrotetrazolium
-
-
-
?
L-serine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
55% activity compared to L-proline
-
-
?
L-threonine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
66% activity compared to L-proline
-
-
?
L-threonine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
?
L-valine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
33% activity compared to L-proline
-
-
?
sarcosine + acceptor + H2O
? + reduced acceptor
-
-
-
-
?
additional information
?
-
glycine + 2,6-dichlorophenolindophenol + H2O

? + reduced 2,6-dichlorophenolindophenol
-
52% activity compared to L-proline
-
-
?
glycine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
52% activity compared to L-proline
-
-
?
L-alanine + 2,6-dichlorophenolindophenol + H2O

? + reduced 2,6-dichlorophenolindophenol
-
48% activity compared to L-proline
-
-
?
L-alanine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
48% activity compared to L-proline
-
-
?
L-alanine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O

?
-
weak activity
-
-
?
L-alanine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
?
-
weak activity
-
-
?
L-arginine + 2,6-dichlorophenolindophenol + H2O

? + reduced 2,6-dichlorophenolindophenol
-
62% activity compared to L-proline
-
-
?
L-arginine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
62% activity compared to L-proline
-
-
?
L-histidine + 2,6-dichlorophenolindophenol + H2O

? + reduced 2,6-dichlorophenolindophenol
-
72% activity compared to L-proline
-
-
?
L-histidine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
72% activity compared to L-proline
-
-
?
L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O

? + reduced 2,6-dichlorophenolindophenol
-
72% activity compared to L-proline
-
-
?
L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
72% activity compared to L-proline
-
-
?
L-proline + 2,6-dichlorophenolindophenol

(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
artificial electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
artificial electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
artificial electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O

(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O

DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
100% activity
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
L-proline is the most preferred substrate and 2,6-dichlorophenolindophenol is the most preferred electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
L-proline is the most preferred substrate and 2,6-dichlorophenolindophenol is the most preferred electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O

DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
-
-
-
?
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
-
-
-
?
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O

DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
9% activity compared to 2,6-dichlorophenolindophenol
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
9% activity compared to 2,6-dichlorophenolindophenol
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
?
L-proline + acceptor

(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O

(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
ir
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
highly specific for L-form of proline
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
preferred substrate
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + cytochrome c + H2O

(S)-1-pyrroline-5-carboxylate + reduced cytochrome c
-
-
-
-
?
L-proline + cytochrome c + H2O
(S)-1-pyrroline-5-carboxylate + reduced cytochrome c
-
-
-
?
L-proline + FAD

(S)-1-pyrroline-5-carboxylate + FADH2
-
-
-
?
L-proline + FAD
(S)-1-pyrroline-5-carboxylate + FADH2
-
-
-
-
r
L-proline + ferricyanide

DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
93% activity compared to 2,6-dichlorophenolindophenol
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
93% activity compared to 2,6-dichlorophenolindophenol
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-threonine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O

?
-
weak activity
-
-
?
L-threonine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
?
-
weak activity
-
-
?
additional information

?
-
-
no activity with: D-proline, glycine, L-glutamate, L-aspartate, L-arginine, L-lysine, L-serine, L-leucine, L-alanine, L-threonine, L-methionine,L-tryptophan, L-tyrosine
-
-
?
additional information
?
-
-
no activity with D-proline, L-tryptophan, L-glutamate, and L-aspartate
-
-
?
additional information
?
-
-
no activity with D-proline, L-tryptophan, L-glutamate, and L-aspartate
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, L-arginine, L-aspartate, and glycine are inert as substrates
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, L-arginine, L-aspartate, and glycine are inert as substrates
-
-
?
additional information
?
-
-
sarcosine, D-proline, L-ornithine, L-glutamate, L-arginine, L-serine, L-leucine, L-valine, and L-alanine are inert as substrates. NAD+, NADP+, and bovine heart cytochrome c are inert as electron acceptors
-
-
?
additional information
?
-
-
sarcosine, D-proline, L-ornithine, L-glutamate, L-arginine, L-serine, L-leucine, L-valine, and L-alanine are inert as substrates. NAD+, NADP+, and bovine heart cytochrome c are inert as electron acceptors
-
-
?
additional information
?
-
-
does not reduce NAD+
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
-
isoform PDH1, no substrate: L-hydroxyproline, D-proline, cis-4-hydroxy-D-proline, L-2-pyrrolidone-5-carboxylate, pyrrole-5-carboxylate, pipecolic acid
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
-
isoform PDH1, no substrate: L-hydroxyproline, D-proline, cis-4-hydroxy-D-proline, L-2-pyrrolidone-5-carboxylate, pyrrole-5-carboxylate, pipecolic acid
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
trans-4-hydroxy-L-proline, cis-4-hydroxy-L-proline, L-azetidine-2-carboxylic acid, and L-pipecolinic acid are no substrates of PRODH
-
-
?
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0.026 - 3.4
2,6-dichlorophenolindophenol
4
3,4-dehydro-L-proline
in 50 mM Tris-HCl, at 25ưC
0.028 - 0.155
coenzyme Q1
6.05
L-hydroxyproline
-
in 200 mM Tris-HCl buffer (pH 8.0), at 50ưC
212.3
L-pipecolic acid
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 80ưC
0.14
oxidized 2,6-dichlorophenolindophenol
-
pH 6.5, 50ưC
additional information
additional information
-
3.4
2,6-dichlorophenolindophenol

pH 7.1, 25ưC, recombinant wild-type enzyme
0.026
2,6-dichlorophenolindophenol
-
with L-proline as cosubstrate, in 200 mM Tris-HCl buffer (pH 8.0), at 50ưC
0.03
2,6-dichlorophenolindophenol
-
isoform PDH1
0.035
2,6-dichlorophenolindophenol
-
with L-hydroxyproline as cosubstrate, in 200 mM Tris-HCl buffer (pH 8.0), at 50ưC
0.155
coenzyme Q1

wild type enzyme, at 23ưC, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
0.028
coenzyme Q1
mutant enzyme G63A, at 23ưC, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
0.032
coenzyme Q1
mutant enzyme E64A, at 23ưC, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
6.5
L-proline

pH 7.4, 45ưC, recombinant wild-type enzyme, Haldane kinetics
9.9
L-proline
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 60ưC
11.5
L-proline
pH 7.4, 25ưC, recombinant wild-type enzyme, Michaelis-Menten kinetics
35
L-proline
-
at pH 8.5 and 30ưC
13
L-proline
with O2 as cosubstrate, in 50 mM Tris-HCl, at 25ưC
14.46
L-proline
-
mutant enzyme H225Q, in 100 mM potassium phosphate buffer, pH 7.5, at 60ưC
16.5
L-proline
pH 7.4, 25ưC, recombinant wild-type enzyme, Haldane kinetics
19.67
L-proline
-
mutant enzyme H225A, in 100 mM potassium phosphate buffer, pH 7.5, at 60ưC
19.9
L-proline
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 70ưC
27
L-proline
with 2,6-dichlorophenolindophenol as cosubstrate, in 50 mM Tris-HCl, at 25ưC
30.8
L-proline
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 80ưC
31
L-proline
-
in 100 mM TrisĆ¢ĀĀHCl, 10 mM MgCl2, 10% (v/v) glycerol, pH 8.5, at 25ưC
33
L-proline
-
without addition of ADP
33.8
L-proline
pH 7.4, 45ưC, recombinant mutant F10E/L12E, Haldane kinetics
3.5
L-proline
TPpdhB2 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50ưC
50
L-proline
mutant enzyme E64A, at 23ưC, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
67.6
L-proline
pH 7.4, 25ưC, recombinant mutant F10E/L12E, Michaelis-Menten kinetics
175.1
L-proline
pH 7.4, 25ưC, recombinant mutant F10E/L12E, Haldane kinetics
230
L-proline
-
DELTAL428 mutant enzyme, at pH 8.0 and 50ưC
290
L-proline
wild type enzyme, at 23ưC, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
384
L-proline
mutant enzyme G63A, at 23ưC, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
860
L-proline
pH 7.1, 25ưC, recombinant mutant Y203F
5.6
L-proline
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 40ưC
0.174
L-proline
-
PF1798 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50ưC
0.28
L-proline
-
pH 6.5, 50ưC
0.28
L-proline
-
wild type enzyme, at pH 8.0 and 50ưC
0.334
L-proline
-
PH1751 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50ưC
0.47
L-proline
-
in 10 mM MOPS, pH 7.5, at 25ưC
0.689
L-proline
-
PF1246 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50ưC
1.46
L-proline
-
TK0117 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50ưC
1.67
L-proline
-
in 200 mM Tris-HCl buffer (pH 8.0), at 50ưC
1.95
L-proline
-
mutant enzyme Y215F, in 100 mM potassium phosphate buffer, pH 7.5, at 60ưC
2 - 6
L-proline
-
in presence of ADP
2 - 6
L-proline
-
depends on assay method
2.38
L-proline
-
TK0122 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50ưC
2.46
L-proline
TPpdhB (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50ưC
2.5 - 5
L-proline
-
PF1364 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50ưC
4
L-proline
-
isoform PDH1
5.3
L-proline
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 50ưC
5.7
L-proline
pH 7.1, 25ưC, recombinant wild-type enzyme
additional information
additional information

Michaelis-Menten and Haldane kinetics
-
additional information
additional information
Michaelis-Menten kinetics, steady-state kinetics and pre-steady-state kinetics of wild-type and mutant enzymes, stopped-flow kinetic isotope effects, bisubstrate kinetic analysis, overview
-
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42
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Nicotiana tabacum
-
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Thermus thermophilus (Q72IB8)
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55
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282
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Thermus thermophilus (Q72IB8)
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Homo sapiens
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Nicotiana tabacum (Q6GUH3), Nicotiana tabacum (Q6GUH4)
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43
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Nicotiana tabacum
-
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64
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Bradyrhizobium japonicum (Q89E26)
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Crystallization and preliminary X-ray analysis of a novel dye-linked L-proline dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix
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66
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89
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Deinococcus radiodurans (Q9RW55)
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287
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Aeropyrum pernix
brenda
Mohammadi, H.S.; Omidinia, E.
Process integration for the recovery and purification of recombinant Pseudomonas fluorescens proline dehydrogenase using aqueous two-phase systems
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929
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Pseudomonas fluorescens
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Proline dehydrogenase contributes to pathogen defense in Arabidopsis
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155
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Arabidopsis thaliana
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Tallarita, E.; Pollegioni, L.; Servi, S.; Molla, G.
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Homo sapiens
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Satomura, T.; Hara, Y.; Suye, S.; Sakuraba, H.; Ohshima, T.
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Aeropyrum pernix
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Kinetic and isotopic characterization of L-proline dehydrogenase from Mycobacterium tuberculosis
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Mycobacterium tuberculosis (O50444), Mycobacterium tuberculosis H37Rv (O50444)
brenda
Monteoliva, M.I.; Rizzi, Y.S.; Cecchini, N.M.; Hajirezaei, M.R.; Alvarez, M.E.
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21
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Arabidopsis thaliana
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Mohammadi, H.S.; Omidinia, E.
Process integration for the recovery and purification of recombinant Pseudomonas fluorescens proline dehydrogenase using aqueous two-phase systems
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929
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Pseudomonas fluorescens
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Huijbers, M.; van Berkel, W.
A more polar N-terminal helix releases MBP-tagged Thermus thermophilus proline dehydrogenase from tetramer-polymer self-association
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134
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Thermus thermophilus (Q72IB8)
-
brenda