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Information on EC 1.7.1.10 - hydroxylamine reductase (NADH)
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1.7.1.10 hydroxylamine reductase (NADH)IUBMB Comments
Also acts on some hydroxamates.
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
pg0893, nadh-hydroxylamine reductase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ammonium dehydrogenase
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-
-
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EC 1.6.6.11
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formerly
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hybrid cluster protein
N-hydroxy amine reductase
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-
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NADH-hydroxylamine reductase
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PG0893
reductase, hydroxylamine
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-
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hybrid cluster protein
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formerly primane protein, displays hydroxylamine reductase activity
hybrid cluster protein
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formerly primane protein, displays hydroxylamine reductase activity
hybrid cluster protein
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formerly primane protein, displays hydroxylamine reductase activity
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
NH3 + NAD+ + H2O = hydroxylamine + NADH + H+
also acts on hydroxamates
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NH3 + NAD+ + H2O = hydroxylamine + NADH + H+
also acts on hydroxamates
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NH3 + NAD+ + H2O = hydroxylamine + NADH + H+
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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-
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redox reaction
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reduction
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-
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-
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SYSTEMATIC NAME
IUBMB Comments
ammonium:NAD+ oxidoreductase
Also acts on some hydroxamates.
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CAS REGISTRY NUMBER
COMMENTARY
9032-06-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N-hydroxy-2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine + NADH
2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine + NAD+ + H2O
N-hydroxy-2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline + NADH
2-amino-3-methylimidazo[4,5-f]quinoxaline + NAD+ + H2O
N-hydroxy-4,4'-methylenebis(2-chloroaniline) + NADH
4,4'-methylenebis(2-chloroaniline) + NAD+
N-methyl-N-benzyl-hydroxylamine + NADH
N-methylbenzylamine + NAD+ + H2O
-
-
-
?
N-methyl-N-n-octyl-hydroxylamine + NADH
N-methyl-n-octylamine + NAD+ + H2O
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-
-
?
anthranillic hydroxamate + NADH + H+
anthranilamide + NAD+ + H2O
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activity in liver mitochondria
-
?
anthranillic hydroxamate + NADH + H+
anthranilamide + NAD+ + H2O
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activity in liver mitochondria
-
?
anthranillic hydroxamate + NADH + H+
anthranilamide + NAD+ + H2O
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activity in liver mitochondria
-
?
anthranillic hydroxamate + NADH + H+
anthranilamide + NAD+ + H2O
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activity in liver mitochondria
-
?
anthranillic hydroxamate + NADH + H+
anthranilamide + NAD+ + H2O
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activity in liver mitochondria
-
?
anthranillic hydroxamate + NADH + H+
anthranilamide + NAD+ + H2O
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activity in liver mitochondria
-
?
anthranillic hydroxamate + NADH + H+
anthranilamide + NAD+ + H2O
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activity in liver mitochondria
-
?
anthranillic hydroxamate + NADH + H+
anthranilamide + NAD+ + H2O
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activity in liver mitochondria
-
?
anthranillic hydroxamate + NADH + H+
anthranilamide + NAD+ + H2O
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activity in liver mitochondria
-
?
anthranillic hydroxamate + NADH + H+
anthranilamide + NAD+ + H2O
Pigeon
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activity in liver mitochondria
-
?
anthranillic hydroxamate + NADH + H+
anthranilamide + NAD+ + H2O
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activity in liver mitochondria
-
?
benzamidoxime + NADH + H+
benzamidine + NAD+ + H2O
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reconstituted enzyme complex from liver microsomes consisting of NADH, cytochrome b5, cytochrome b5 reductase, a P450 isoenzyme and phosphatidylcholin, 40% activity with NADPH
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?
guanoxabenz + NADH
?
-
reconstituted enzyme complex from liver microsomes consisting of NADH, cytochrome b5, cytochrome b5 reductase, a P450 isoenzyme and phosphatidylcholin, 40% activity with NADPH
-
-
?
hydroxylamine + NADH
NH3 + H2O + NAD+
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only native, not recombinant protein, recombinant protein lacks NADH oxidoreductase activity
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-
?
hydroxylamine + NADH
NH3 + H2O + NAD+
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only native, not recombinant protein, recombinant protein lacks NADH oxidoreductase activity
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-
?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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100% activity with NADH, 46% with NADPH, 117% with FADH2, 105% with FMNH2, 220% with methylviologen, 210% with benzyl viologen, 57% with Na2S2O4
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?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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anaeorobically in the presence of methyl viologen
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-
?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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NADH is 1.5fold more effective than NADPH
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?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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no other substances beside nitrite and dinitrobenzene stimulate oxidation of NADPH
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ir
hydroxylamine + NADH
NH3 + NAD+ + H2O
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no activity with O-methylhydroxylamine, hydrazine, nitrophenylhydroxylamine and salicylhydroxamic acid
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ir
hydroxylamine + NADH
NH3 + NAD+ + H2O
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NADPH is 2fold more effective than NADH
-
?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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involved in pathway of nitrate reduction
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?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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enzyme also reduces anthranilic hydroxamic acid and nicotinic hydroxamic acid, affinity is about twenty times lower than for hydroxylamine
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?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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very low activity with phenylhydroxylamine
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?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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no reduction of N,N-diethyl or O-methyl compounds
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?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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NADH is 1.5fold more effective than NADPH
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?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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possible identity of hydroxylamine reductase and the enzyme which reduces anthranilic hydroxamic acid to the amide
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?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation
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?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation
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?
hydroxylamine + NADH
NH3 + NAD+ + H2O
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in the presence of a reduced viologen dye
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-
?
N-hydroxy-1-naphthylamine + NADH
1-naphthylamine + NAD+ + H2O
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activity in liver microsomes
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?
N-hydroxy-2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine + NADH
2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine + NAD+ + H2O
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-
-
?
N-hydroxy-2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine + NADH
2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine + NAD+ + H2O
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activity in liver microsomes, primary rat hepatocytes and human HepG2 cells
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?
N-hydroxy-2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline + NADH
2-amino-3-methylimidazo[4,5-f]quinoxaline + NAD+ + H2O
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-
-
?
N-hydroxy-2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline + NADH
2-amino-3-methylimidazo[4,5-f]quinoxaline + NAD+ + H2O
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activity in liver microsomes
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?
N-hydroxy-2-amino-alpha-carboline + NADH
2-amino-alpha-carboline + NAD+ + H2O
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-
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?
N-hydroxy-2-amino-alpha-carboline + NADH
2-amino-alpha-carboline + NAD+ + H2O
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activity in liver microsomes
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?
N-hydroxy-2-aminofluorene + NADH
2-aminofluorene + NAD+ + H2O
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activity in liver microsomes
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?
N-hydroxy-2-naphthylamine + NADH
2-naphthylamine + NAD+ + H2O
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activity in liver microsomes
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?
N-hydroxy-4,4'-methylenebis(2-chloroaniline) + NADH
4,4'-methylenebis(2-chloroaniline) + NAD+
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-
-
?
N-hydroxy-4,4'-methylenebis(2-chloroaniline) + NADH
4,4'-methylenebis(2-chloroaniline) + NAD+
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activity in liver microsomes
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?
N-hydroxy-4-aminoazobenzene + NADH
4-aminoazobenzene + NAD+ + H2O
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-
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?
N-hydroxy-4-aminoazobenzene + NADH
4-aminoazobenzene + NAD+ + H2O
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activity in liver microsomes
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?
N-hydroxy-4-aminobiphenyl + NADH
4-aminobiphenyl + NAD+ + H2O
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activity in liver microsomes
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?
N-hydroxy-aniline + NADH
aniline + NAD+ + H2O
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activity in liver microsomes
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?
N-hydroxy-arylamines + NADH
N-arylamines + NAD+ + H2O
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enzyme may be involved in detoxification pathway of carcinogenic aromatic and heterocyclic amines
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?
N-hydroxy-arylamines + NADH
N-arylamines + NAD+ + H2O
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enzyme may be involved in detoxification pathway of carcinogenic aromatic and heterocyclic amines
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?
N-hydroxydebrisoquine + NADH
debrisquine + NAD+ + H2O
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reconstituted enzyme complex from liver microsomes consisting of NADH, cytochrome b5, cytochrome b5 reductase, a P450 isoenzyme and phosphatidylcholin, 40% activity with NADPH
-
?
NO2- + NADH
NH3 + H2O + NAD+
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enzyme has both nitrite reductase and hydroxylamine reductase activity, main function may be nitrite reduction
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?
sulphamethoxazole hydroxylamine + NADH + H+
sulphamethoxazole + NAD+ + H2O
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activity in liver microsomes, NADH is preferred
antimicrobial for the treatment of gastrointestinal, urinary and respiratory pathogens
?
sulphamethoxazole hydroxylamine + NADH + H+
sulphamethoxazole + NAD+ + H2O
-
activity in liver microsomes, NADH is preferred
antimicrobial for the treatment of gastrointestinal, urinary and respiratory pathogens
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
hydroxylamine + NADH
NH3 + NAD+ + H2O
-
involved in pathway of nitrate reduction
-
?
hydroxylamine + NADH
NH3 + NAD+ + H2O
-
involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation
-
-
?
hydroxylamine + NADH
NH3 + NAD+ + H2O
-
involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation
-
-
?
N-hydroxy-arylamines + NADH
N-arylamines + NAD+ + H2O
-
enzyme may be involved in detoxification pathway of carcinogenic aromatic and heterocyclic amines
-
?
N-hydroxy-arylamines + NADH
N-arylamines + NAD+ + H2O
-
enzyme may be involved in detoxification pathway of carcinogenic aromatic and heterocyclic amines
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
purified enzyme: absolute requirement for an unidentified, dissociable, heat-stable, organic factor obtained from soybean leaf extract, no flavin requirement
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CN-
-
stimulating at concentrations above 5 mM, inhibitory after 1 h preincubation
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate
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1.5 mM, 68% inhibition, hydroxylamine protects
nitrite
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nitrite and hydroxylamine reductase activity are associated with the same enzyme, nitrite and hydroxylamine are competitive for the same binding site
potassium thiocyanate
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0.1 mM, 90% inhibition of hydroxylamine reductase activity, nitrite reductase activity is not inhibited
8-hydroxyquinoline
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0.1 mM, 50% inhibition of hydroxylamine reductase activity
p-chloromercuribenzoate
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0.1 mM, 67% inhibition of hydroxylamine reductase activity
p-chloromercuribenzoate
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0.05 mM, complete inhibition, subsequent addition of 3 mM cysteine restores the activity
additional information
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not inhibited by a nitrogen atmosphere; not inhibited by azide, CO, dextromethorphan or erythromycin
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additional information
-
not inhibited by azide, CO, dextromethorphan or erythromycin
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY