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Information on EC 2.1.1.183 - 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase
for references in articles please use BRENDA:EC2.1.1.183
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EC Tree 2 Transferases
2.1 Transferring one-carbon groups
2.1.1 Methyltransferases
2.1.1.183 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase
IUBMB Comments
DIM1 is involved in pre-rRNA processing .
The enzyme appears in viruses and cellular organisms
- 2.1.1.183
- pombe
-
schizosaccharomyces
-
tri-snrnps
-
mitosis
- pre-rrnas
-
nucleolar
-
fission
- collagen
- ribonucleoprotein
- oncogene
-
spindle
-
thioredoxin-like
-
pre-ribosomal
-
anaphase-promoting
-
metastasis-associated
-
salivary
- pirin
-
spliceosomal
- transketolase
-
v-ha-ras
-
adenoid
-
cyclosome
-
snrnp
-
cystic
Reaction Schemes
show4
+
=
4
+
4
+
adenine1779/adenine1780 in 18S rRNA
=
4
+
N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA
Synonyms
dim1p, scdim1, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
EC 2.1.1.48
-
-
formerly, part transferred
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA = 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase
DIM1 is involved in pre-rRNA processing [1].
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4 S-adenosyl-L-methionine + adenine1779/1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA
Substrates: -
Products: -
Products: -
?
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenosine1779/N6-dimethyladenine1780 in 18S rRNA
Substrates: isolation of mutations that uncouple the enzymatic function of Dim1p in methylation from its involvement in prerRNA processing
Products: -
Products: -
?
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenosine1779/N6-dimethyladenosine1780 in 18S rRNA
Substrates: neither the bacterial nor the archaeal ortholog could complement for the eukaryotic Dim1. This might be related to the secondary, non-methyltransferase function that Dim1 is known to play in eukaryotic ribosomal maturation
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme catalyzed methylation of Escherichia coli 30S ribosomes. The enzyme produces a mixture of N6-methyladenine and 6-dimethyladenine (1.4 times as much N6-methyladenine as N6-dimethyladenine). 80% of the potential sites are methylated after 2 h. Under the assay conditions, ScDim1 releases the N6-methyladenine intermediate, which is subsequently converted to the N6-dimethyladenine product after an additional binding event
Products: -
Products: -
?
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA
Substrates: -
Products: -
Products: -
?
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA
Substrates: Dim1p is required for pre-ribosomal RNA processing
Products: -
Products: -
?
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA
Substrates: presence of the 6-dimethyladenine1779/6-dimethyladenine1780 is detected by primer extension
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA
Substrates: Dim1p is required for pre-ribosomal RNA processing
Products: -
Products: -
?
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenosine1779/N6-dimethyladenine1780 in 18S rRNA
Substrates: isolation of mutations that uncouple the enzymatic function of Dim1p in methylation from its involvement in prerRNA processing
Products: -
Products: -
?
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenosine1779/N6-dimethyladenosine1780 in 18S rRNA
Substrates: neither the bacterial nor the archaeal ortholog could complement for the eukaryotic Dim1. This might be related to the secondary, non-methyltransferase function that Dim1 is known to play in eukaryotic ribosomal maturation
Products: -
Products: -
?
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Neoplasm Metastasis
Proteomics analysis of differentially expressed metastasis-associated proteins in adenoid cystic carcinoma cell lines of human salivary gland.
Neoplasms
Proteomics analysis of differentially expressed metastasis-associated proteins in adenoid cystic carcinoma cell lines of human salivary gland.
Neoplasms
[Analysis of differentially expressed lung metastasis-associated proteins in adenoid cystic carcinoma cell lines]
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Dim1 in eukaryotic organisms is an essential member of the processome, a multifactor assembly that does not exist in bacteria
-
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
DIM1 is involved in pre-rRNA processing
malfunction
a conditional GAL1O::diml strain is constructed to allow the depletion of Dimlp from the cell. During depletion, dimethylation of the pre-rRNA is progressively inhibited and pre-rRNA processing at cleavage sites A1 and A2 is concomitantly lost. In consequence, the mature 18s rRNA and its 20s precursor drastically underaccumulate. This has the effect of preventing the synthesis of nonmethylated rRNA
malfunction
isolation of mutations that uncouple the enzymatic function of Dim1p in methylation from its involvement in prerRNA processing
malfunction
neither the bacterial nor the archaeal ortholog could complement for the eukaryotic Dim1. This might be related to the secondary, non-methyltransferase function that Dim1 is known to play in eukaryotic ribosomal maturation
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). DIM1_ASHGO
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
319
0
35818
Swiss-Prot
other Location (Reliability: 2)
DIM1_BOVIN
313
0
35167
Swiss-Prot
Mitochondrion (Reliability: 5)
DIM1_CAEEL
308
0
34610
Swiss-Prot
other Location (Reliability: 3)
DIM1_CANGA
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)
317
0
35920
Swiss-Prot
other Location (Reliability: 2)
DIM1_CHATD
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
384
0
42546
Swiss-Prot
other Location (Reliability: 2)
DIM1_DEBHA
Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)
327
0
36534
Swiss-Prot
Mitochondrion (Reliability: 5)
DIM1_DICDI
314
0
35341
Swiss-Prot
other Location (Reliability: 2)
DIM1_DROME
306
0
35048
Swiss-Prot
other Location (Reliability: 2)
DIM1_HUMAN
313
0
35236
Swiss-Prot
other Location (Reliability: 4)
DIM1_KLULA
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
320
0
35763
Swiss-Prot
other Location (Reliability: 2)
DIM1_MACFA
313
0
35221
Swiss-Prot
other Location (Reliability: 4)
DIM1_MOUSE
313
0
35274
Swiss-Prot
Mitochondrion (Reliability: 5)
DIM1_SCHPO
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
307
0
34678
Swiss-Prot
Mitochondrion (Reliability: 5)
DIM1_YARLI
Yarrowia lipolytica (strain CLIB 122 / E 150)
317
0
35351
Swiss-Prot
other Location (Reliability: 4)
DIM1_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
318
0
35951
Swiss-Prot
other Location (Reliability: 3)
A0A0L0SJE7_ALLM3
Allomyces macrogynus (strain ATCC 38327)
531
0
57655
TrEMBL
Secretory Pathway (Reliability: 2)
A0A4S4LAS4_9AGAM
772
0
86418
TrEMBL
Mitochondrion (Reliability: 4)
A0A1J1HCB6_PLARL
142
0
16817
TrEMBL
other Location (Reliability: 3)
A0A0L0STC5_ALLM3
Allomyces macrogynus (strain ATCC 38327)
531
0
57858
TrEMBL
Secretory Pathway (Reliability: 2)
A0A1V2L9Z1_CYBFA
869
0
95363
TrEMBL
other Location (Reliability: 4)
A0A8J1YT22_9TREE
844
0
94053
TrEMBL
other Location (Reliability: 1)
A0A8M1N1K3_DANRE
306
0
34668
TrEMBL
other Location (Reliability: 2)
A0A653H2F5_9APIC
142
0
16848
TrEMBL
other Location (Reliability: 2)
Q8I5A5_PLAF7
139
0
16539
TrEMBL
other Location (Reliability: 2)
C7GSG6_YEAS2
Saccharomyces cerevisiae (strain JAY291)
318
0
35951
TrEMBL
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
E85A
the altered protein is able to complement for Dim1 but yields a protein with no catalytic activity as determined by primer extension of 18S rRNA. The expected primer extension stops at 1779 and 1780 are missing in rRNA extracted from the strain expressing catalytically inactive ScDim1. The single mutant shows no change in growth rate of yeast at 18, 25, 30, and 37°C
additional information
to delineate regions of the eukaryotic Dim1 critical to its function, KsgA/Dim1 chimeras are created and tested. Construction of six chimeras that result from swapping the two domains of Saccharomyces cerevisiae Dim1, Escherichia coli KsgA, and Methanocaldococcus jannaschii Dim1. Of the chimeras, only one constructed with the N-terminal domain from eukaryotic Dim1 and the C-terminal domain from archaeal Dim1 is able to complement for the eukaryotic Dim1, suggesting that eukaryotic-specific Dim1 function resides in the N-terminal domain, where few structural differences are observed between members of the KsgA/Dim1 family
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Dim1 complements heterologously for ksgA- mutation in Escherichia coli, demonstrating functional equivalence of the two proteins
to delineate regions of the eukaryotic Dim1 critical to its function, KsgA/Dim1 chimeras are created and tested. Construction of six chimeras that result from swapping the two domains of Saccharomyces cerevisiae Dim1, Escherichia coli KsgA, and Methanocaldococcus jannaschii Dim1. Of the chimeras, only one constructed with the N-terminal domain from eukaryotic Dim1 and the C-terminal domain from archaeal Dim1 is able to complement for the eukaryotic Dim1
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lafontaine, D.; Vandenhaute, J.; Tollervey, D.
The 18S rRNA dimethylase Dim1p is required for pre-ribosomal RNA processing in yeast
Genes Dev.
9
2470-2481
1995
Saccharomyces cerevisiae (C7GSG6), Saccharomyces cerevisiae
brenda
Lafontaine, D.; Delcour, J.; Glasser, A.L.; Desgres, J.; Vandenhaute, J.
The DIM1 gene responsible for the conserved m6(2)Am6(2)A dimethylation in the 3'-terminal loop of 18 S rRNA is essential in yeast
J. Mol. Biol.
241
492-497
1994
Saccharomyces cerevisiae (C7GSG6)
brenda
Pulicherla, N.; Pogorzala, L.A.; Xu, Z.; O'Farrell, H.C.; Musayev, F.N.; Scarsdale, J.N.; Sia, E.A.; Culver, G.M.; Rife, J.P.
Structural and functional divergence within the Dim1/KsgA family of rRNA methyltransferases
J. Mol. Biol.
391
884-893
2009
Saccharomyces cerevisiae (C7GSG6)
brenda
Lafontaine, D.L.; Preiss, T.; Tollervey, D.
Yeast 18S rRNA dimethylase Dim1p: a quality control mechanism in ribosome synthesis?
Mol. Cell. Biol.
18
2360-2370
1998
Saccharomyces cerevisiae (C7GSG6), Saccharomyces cerevisiae
brenda
O'Farrell, H.C.; Pulicherla, N.; Desai, P.M.; Rife, J.P.
Recognition of a complex substrate by the KsgA/Dim1 family of enzymes has been conserved throughout evolution
RNA
12
725-733
2006
Saccharomyces cerevisiae (C7GSG6)
brenda
Housen, I.; Demonte, D.; Lafontaine, D.; Vandenhaute, J.
Cloning and characterization of the KlDIM1 gene from Kluyveromyces lactis encoding the m2(6)A dimethylase of the 18S rRNA
Yeast
13
777-781
1997
Kluyveromyces lactis (P78697), Kluyveromyces lactis
brenda
EXTERNAL LINKS (specific for EC-Number 2.1.1.183)
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Release 2023.1 (January 2023)
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