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UDP-alpha-D-glucose + isoliquiritigenin
UDP + isoliquiritigenin 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + 4,2',4',6'-tetrahydroxychalcone
UDP + ?
-
-
-
-
?
UDP-glucose + an isoflavone
UDP + an isoflavone 7-O-beta-D-glucoside
-
-
-
?
UDP-glucose + apigenin
UDP + apigenin 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + aureusidin
UDP + aureusidin 6-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + baicalein
UDP + baicalein 7-O-beta-D-glucoside
-
very low activity
-
-
?
UDP-glucose + biochanin A
UDP + biochanin A 7-O-beta-D-glucoside
UDP-glucose + daidzein
UDP + daidzein 7-O-beta-D-glucoside
UDP-glucose + eriodictyol
UDP + eriodictyol 7-O-beta-D-glucoside
-
-
-
?
UDP-glucose + esculetin
UDP + esculetin 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + ferulic acid
UDP + 1-O-feruloyl-beta-D-glucose
-
low activity
-
-
?
UDP-glucose + formononetin
UDP + formononetin 7-O-beta-D-glucoside
UDP-glucose + genistein
UDP + genistein 7-O-beta-D-glucoside
UDP-glucose + isovitexin
UDP + isovitexin 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
UDP-glucose + liquiritigenin
UDP + liquiritigenin 7-O-beta-D-glucoside
-
very low activity
-
-
?
UDP-glucose + naringenin
UDP + naringenin 7-O-beta-D-glucoside
UDP-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
UDP-glucose + salicylic acid
UDP + salicylic acid 7-O-beta-D-glucoside
-
very low activity
-
-
?
UDP-glucose + scopoletin
UDP + scopoletin 7-O-beta-D-glucoside
-
low activity
-
-
?
additional information
?
-
UDP-glucose + biochanin A
UDP + biochanin A 7-O-beta-D-glucoside
-
-
-
?
UDP-glucose + biochanin A
UDP + biochanin A 7-O-beta-D-glucoside
-
-
-
?
UDP-glucose + daidzein
UDP + daidzein 7-O-beta-D-glucoside
-
poor substrate
-
-
?
UDP-glucose + daidzein
UDP + daidzein 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + daidzein
UDP + daidzein 7-O-beta-D-glucoside
-
-
-
?
UDP-glucose + daidzein
UDP + daidzein 7-O-beta-D-glucoside
-
i.e. daidzin
-
?
UDP-glucose + daidzein
UDP + daidzein 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + daidzein
UDP + daidzein 7-O-beta-D-glucoside
36% of the activity with genistein
-
-
?
UDP-glucose + daidzein
UDP + daidzein 7-O-beta-D-glucoside
-
-
i.e. daidzin
-
?
UDP-glucose + daidzein
UDP + daidzein 7-O-beta-D-glucoside
-
high activity
i.e. daidzin
-
?
UDP-glucose + formononetin
UDP + formononetin 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + formononetin
UDP + formononetin 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + formononetin
UDP + formononetin 7-O-beta-D-glucoside
-
best substrate
-
-
?
UDP-glucose + formononetin
UDP + formononetin 7-O-beta-D-glucoside
-
-
i.e. ononin
-
?
UDP-glucose + genistein
UDP + genistein 7-O-beta-D-glucoside
-
poor substrate
-
-
?
UDP-glucose + genistein
UDP + genistein 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + genistein
UDP + genistein 7-O-beta-D-glucoside
-
-
-
?
UDP-glucose + genistein
UDP + genistein 7-O-beta-D-glucoside
-
i.e. genistin
-
?
UDP-glucose + genistein
UDP + genistein 7-O-beta-D-glucoside
-
-
-
?
UDP-glucose + genistein
UDP + genistein 7-O-beta-D-glucoside
-
-
i.e. genistin
-
?
UDP-glucose + genistein
UDP + genistein 7-O-beta-D-glucoside
-
best substrate
i.e. genistin
-
?
UDP-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
-
-
?
UDP-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + naringenin
UDP + naringenin 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + naringenin
UDP + naringenin 7-O-beta-D-glucoside
-
low activity
-
-
?
UDP-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
-
-
?
UDP-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
-
-
-
?
additional information
?
-
caffeic acid and esculetin do not react with the enzyme. AtGT-2 prefers flavonene to flavone or flavonol, both of which contain a double bond in the C ring. The relative conversion rate is 100% for eriodictyol, 71.6% for naringenin, 29.6% for apigenin, 92.1% for luteolin, 78.9% for kaempferol and 64.1% for quercetin.
-
-
?
additional information
?
-
-
caffeic acid and esculetin do not react with the enzyme. AtGT-2 prefers flavonene to flavone or flavonol, both of which contain a double bond in the C ring. The relative conversion rate is 100% for eriodictyol, 71.6% for naringenin, 29.6% for apigenin, 92.1% for luteolin, 78.9% for kaempferol and 64.1% for quercetin.
-
-
?
additional information
?
-
-
none of the following phenolics serve as glucosyl acceptors: cyanidin, trans-p-coumaric acid, caffeic acid, benzoic acid, m- and p-hydroybenzoic acid, salicylic acid, salicyl alcohol and hydroquinone.
-
-
?
additional information
?
-
-
the relative activites for UDP-glucuronic acid and for UDP-galactose is 0% and for UDP-glucose 12%, all tested with genistein as glycosyl acceptor
-
-
?
additional information
?
-
-
GmUGT1 is active with UDP-galactose and UDP-glucuronic acid as substrates. It displays high specific activities toward isoflavones (genistein and daidzein) to produce their 7-O-beta-D-glucosides, it also shows considerable levels of activities toward other flavonoids such as kaempferol, quercetin, naringenin and apigenin. GmUGT1 is also capable of acting on the flavone luteolin, (S)-equol, a 4',7-isoflavandiol, and (R)-equol
-
-
?
additional information
?
-
GmUGT1 is active with UDP-galactose and UDP-glucuronic acid as substrates. It displays high specific activities toward isoflavones (genistein and daidzein) to produce their 7-O-beta-D-glucosides, it also shows considerable levels of activities toward other flavonoids such as kaempferol, quercetin, naringenin and apigenin. GmUGT1 is also capable of acting on the flavone luteolin, (S)-equol, a 4',7-isoflavandiol, and (R)-equol
-
-
?
additional information
?
-
-
in contrast to GmUGT1, UDP-galactose and UDP-glucuronic acid are essentially inert as substrates for GmUGT3, glucosyl-acceptor substrate specificity, overview. No activity with kaempferol, quercetin, naringenin, apigenin, and luteolin
-
-
?
additional information
?
-
in contrast to GmUGT1, UDP-galactose and UDP-glucuronic acid are essentially inert as substrates for GmUGT3, glucosyl-acceptor substrate specificity, overview. No activity with kaempferol, quercetin, naringenin, apigenin, and luteolin
-
-
?
additional information
?
-
-
in contrast to GmUGT1, UDP-galactose and UDP-glucuronic acid are essentially inert as substrates for GmUGT4, glucosyl-acceptor substrate specificity, overview. No activity with kaempferol, quercetin, naringenin, apigenin, and luteolin
-
-
?
additional information
?
-
in contrast to GmUGT1, UDP-galactose and UDP-glucuronic acid are essentially inert as substrates for GmUGT4, glucosyl-acceptor substrate specificity, overview. No activity with kaempferol, quercetin, naringenin, apigenin, and luteolin
-
-
?
additional information
?
-
-
the enzyme might play a role in defense against external stresses such as UV radiation and pathogen attack
-
-
?
additional information
?
-
-
substrate specificity, the flavonoids kaempferol, apigenin, and quercetin are poor substrates
-
-
?
additional information
?
-
endogenous substrate not known
-
-
?
additional information
?
-
-
endogenous substrate not known
-
-
?
additional information
?
-
no activity with apigenin, kaempferol, luteolin, naringenin, and quercetin
-
-
?
additional information
?
-
-
no activity with apigenin, kaempferol, luteolin, naringenin, and quercetin
-
-
?
additional information
?
-
-
the enzyme is relatively specific for isoflavone aglycones, while flavonoid substrates are poorly accepted. No activity with cyanidin, luteolin, kaempferol, and quercetin, naringenin and apigenin are poor substrates, substrate specificity, overview
-
-
?
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0.00042 - 0.0985
daidzein
0.00042
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.0103
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.0189
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT1
0.0203
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.0299
daidzein
-
pH 8.0, 30°C
0.0985
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.00031
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.00077
genistein
-
E392D mutant, enzyme assay is run at pH 8.5 at 30°C
0.0026
genistein
-
E456A mutant, enzyme assay is run at pH 8.5 at 30°C
0.0027
genistein
-
H359A muant, enzyme assay is run at pH 8.5 at 30°C
0.0027
genistein
-
wild-type, enzyme assay is run at pH 8.5 at 30°C
0.0036
genistein
-
purified enzyme, enzyme assay is run at pH 8.5 at 30°C
0.0045
genistein
-
E376A mutant, enzyme assay is run at pH 8.5 at 30°C
0.0049
genistein
-
H368A mutant, enzyme assay is run at pH 8.5 at 30°C
0.011
genistein
-
E392A mutant, enzyme assay is run at pH 8.5 at 30°C
0.0129
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.016
genistein
-
H15A mutant, enzyme assay is run at pH 8.5 at 30°C
0.017
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT1
0.0204
genistein
-
pH 8.0, 30°C
0.0237
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.0267
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.06
genistein
-
D125A mutant, enzyme assay is run at pH 8.5 at 30°C
0.024
UDP-glucose
-
E392D mutant, enzyme assay is run at pH 8.5 at 30°C
0.027
UDP-glucose
-
H15A mutant, enzyme assay is run at pH 8.5 at 30°C
0.045
UDP-glucose
-
D125A mutant, enzyme assay is run at pH 8.5 at 30°C
0.049
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.0537
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT1
0.0564
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.072
UDP-glucose
-
H359A muant, enzyme assay is run at pH 8.5 at 30°C
0.081
UDP-glucose
-
H368A mutant, enzyme assay is run at pH 8.5 at 30°C
0.082
UDP-glucose
-
E456A mutant, enzyme assay is run at pH 8.5 at 30°C
0.0869
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.13
UDP-glucose
-
wild-type, enzyme assay is run at pH 8.5 at 30°C
0.1637
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.19
UDP-glucose
-
E392A mutant, enzyme assay is run at pH 8.5 at 30°C
0.19
UDP-glucose
-
purified enzyme, enzyme assay is run at pH 8.5 at 30°C
2.1
UDP-glucose
-
E376A mutant, enzyme assay is run at pH 8.5 at 30°C
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additional information
additional information
-
0.1
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.97
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
1.1
daidzein
-
pH 8.0, 30°C
1.22
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
5.75
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT1
5.89
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.0004
genistein
-
E392A mutant, enzyme assay is run at pH 8.5 at 30°C
0.13
genistein
-
H15A mutant, enzyme assay is run at pH 8.5 at 30°C
0.13
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.39
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.41
genistein
-
E456A mutant, enzyme assay is run at pH 8.5 at 30°C
0.59
genistein
-
H359A muant, enzyme assay is run at pH 8.5 at 30°C
0.63
genistein
-
E376A mutant, enzyme assay is run at pH 8.5 at 30°C
0.66
genistein
-
D125A mutant, enzyme assay is run at pH 8.5 at 30°C
0.74
genistein
-
purified enzyme, enzyme assay is run at pH 8.5 at 30°C
0.76
genistein
-
wild-type, enzyme assay is run at pH 8.5 at 30°C
1.1
genistein
-
pH 8.0, 30°C
1.75
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
2
genistein
-
H368A mutant, enzyme assay is run at pH 8.5 at 30°C
3
genistein
-
E392D mutant, enzyme assay is run at pH 8.5 at 30°C
4.34
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT1
6.04
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.11
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
1.41
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
1.72
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
7.65
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
8.79
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT1
additional information
additional information
Kcat/Km for eriodictyol is 2.66 microM-1/mg-1, 1.32 microM-1/mg-1 for kaempferol and 250 microM-1/mg-1 for quercetin. Assay at pH 7.0 and 37°C
-
additional information
additional information
-
Kcat/Km for eriodictyol is 2.66 microM-1/mg-1, 1.32 microM-1/mg-1 for kaempferol and 250 microM-1/mg-1 for quercetin. Assay at pH 7.0 and 37°C
-
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37
daidzein
-
pH 8.0, 30°C
94.8
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
124
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
244
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
291
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT3
304
daidzein
pH 8.5, 30°C, recombinant isozyme GmUGT1
54
genistein
-
pH 8.0, 30°C
136
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
167
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
226
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
255
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT1
409
genistein
pH 8.5, 30°C, recombinant isozyme GmUGT3
0.25
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
2
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
10.5
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
88
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT3
164
UDP-glucose
pH 8.5, 30°C, recombinant isozyme GmUGT1
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0.045
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme
0.049
substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme
0.158
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme
0.209
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme
0.457
substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme
0.465
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme
0.467
substrate daidzein, , pH 8.5, 30°C, purified recombinant enzyme
0.253
substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme
0.253
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme
additional information
Vmax for eriodictyol is 333.3 pkat/mg, 333.3 pkat/mg for kaempferol and 250 pkat/mg for quercetin. Assay at pH 7.0 and 37°C
additional information
-
Vmax for eriodictyol is 333.3 pkat/mg, 333.3 pkat/mg for kaempferol and 250 pkat/mg for quercetin. Assay at pH 7.0 and 37°C
additional information
-
-
additional information
-
as glucosyl acceptor genistein shows the highest activity (100%), daidzein has 73%, formononetin shows 67%, quercetin 32%, kaempferol 19%, 4,2',4',6'-tetrahydoxychalcone 15%, apigenin 14%, aureusidin 5%, esculetin 4% and naringenin shows 3% activity (all tested with UDP-glucose). Enzyme assay is run at pH 8.5 at 30°C
additional information
-
GmUGT1 activity with other flavonoids, overview
additional information
GmUGT1 activity with other flavonoids, overview
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seedling
brenda
seedling
brenda
-
during the riping process the enzyme shows two activity peaks: in green fruits and in full-ripe red fruits
brenda
unifoliate nodes, seedling
brenda
-
-
brenda
exocarp, mesocarp and endocarp
brenda
seedling
brenda
AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems
brenda
-
transient induction by cell suspension culture treatment with yeast extract
brenda
-
-
brenda
-
-
brenda
-
brenda
seedling
brenda
-
seedling
-
brenda
AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems
brenda
-
brenda
-
brenda
seedling
brenda
AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems
brenda
unifoliate leaves, seedling
brenda
-
unifoliate leaves, seedling
-
brenda
AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems
brenda
-
-
brenda
lateral
brenda
seedling
brenda
-
roots of 7-9 day-old seedlings
brenda
lateral and taproot
brenda
the expression of UGT4, an isoflavone-specific 7-O-glucosyltransferase (UGT4), is decreased during the reproductive stages. Expression of UGT4 is high in the vegetative stages, and decreased by over 90% during the reproductive stages
brenda
-
seedling
-
brenda
-
high enzyme expression level
brenda
-
brenda
-
-
brenda
-
-
-
brenda
-
brenda
-
-
brenda
-
-
-
brenda
additional information
real-time RT-PCR tissue expression analysis of GmIF7GT gene homologues 1-6, overview. The transcript level of a less known gene homologue of isoflavone 7-O-glucosyltransferase GmIF7GT4 is significantly higher by about 11fold, in the roots than the gene homologue mIF7GT1 and the other homologues. Transcript levels of other homologues of GmIF7GT are much lower than the transcript levels of GmIF7GT1 or GmIF7GT4, overview
brenda
additional information
-
real-time RT-PCR tissue expression analysis of GmIF7GT gene homologues 1-6, overview. The transcript level of a less known gene homologue of isoflavone 7-O-glucosyltransferase GmIF7GT4 is significantly higher by about 11fold, in the roots than the gene homologue mIF7GT1 and the other homologues. Transcript levels of other homologues of GmIF7GT are much lower than the transcript levels of GmIF7GT1 or GmIF7GT4, overview
brenda
additional information
-
gene GmUGT3 encodes a UGT that is highly specific for isoflavones
brenda
additional information
gene GmUGT3 encodes a UGT that is highly specific for isoflavones
brenda
additional information
-
gene GmUGT4 encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, followed by taproots, cotyledon, and hypocotyls
brenda
additional information
gene GmUGT4 encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, followed by taproots, cotyledon, and hypocotyls
brenda
additional information
-
gene GmUGT9 encodes a UGT that is highly specific for isoflavones, the levels of the GmUGT9 transcripts are highest in roots, followed by taproots, cotyledon, and hypocotyls
brenda
additional information
gene GmUGT9 encodes a UGT that is highly specific for isoflavones, the levels of the GmUGT9 transcripts are highest in roots, followed by taproots, cotyledon, and hypocotyls
brenda
additional information
-
the levels of the most abundant GmUGT1 transcripts in cotyledons are much higher than those in lateral roots
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additional information
the levels of the most abundant GmUGT1 transcripts in cotyledons are much higher than those in lateral roots
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additional information
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the levels of the most abundant GmUGT7 transcripts in cotyledons are much higher than those in lateral roots
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additional information
the levels of the most abundant GmUGT7 transcripts in cotyledons are much higher than those in lateral roots
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additional information
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transcript abundance of gee PlUGT1 is correlated with the accumulation pattern of isoflavone glycosides such as daidzin in Pueraria lobata plants or in cell suspension culture
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evolution
nine different GmUGT cDNAs that are related to GmUGT1 are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones. Gene GmUGT4, a representative of subgroup A, encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, while gene GmUGT1, a representative of subgroup B, encodes an enzyme displaying a broad glucosyl-acceptor specificity and is expressed in the aerial parts, i.e. cotyledons, hypocotyls and pods
evolution
nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview
evolution
nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones
evolution
nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones. Gene GmUGT4, a representative of subgroup A, encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, while gene GmUGT1, a representative of subgroup B, encodes an enzyme displaying a broad glucosyl-acceptor specificity and is expressed in the aerial parts, i.e. cotyledons, hypocotyls and pods
metabolism
the enzyme take part in the isoflavonoid pathway, in silico expression profiles of genes involved in the pathway, isoflavonoid pathway overview
metabolism
the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis
metabolism
the enzyme is involved in the isoflavone biosynthesis, pathway overview. The expressions of UGT4, an isoflavone-specific 7-O-glucosyltransferase, and of ICHG (isoflavone conjugates hydrolyzing beta-glucosidase) are decreased during the reproductive stages. Isoflavone contents are higher in rhizosphere soil than in bulk soil during both vegetative and reproductive stages, and are comparable in the rhizosphere soil between these two stages
physiological function
the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis. Isoflavone conjugates, 7-O-beta-D-glucosides and 7-O-(6''-malonyl-beta-D-glucosides) of daidzein and genistein, accumulate in soybean roots and serve as the stored precursors of isoflavones (aglycons), which play very important roles in the rhizobia-mediated nodulation of this plant
physiological function
the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis. Isoflavone conjugates, 7-O-beta-D-glucosides and 7-O-(6''-malonyl-beta-D-glucosides) of daidzein and genistein, accumulate in soybean roots and serve as the stored precursors of isoflavones (aglycons), which play very important roles in the rhizobia-mediated nodulation of this plant. Isozyme GmUGT4, but not GmUGT1, plays an exclusive role in the conjugation of isoflavones in the lateral roots of the soybean plant
physiological function
the enzyme is involved in the synthesis and secretion of isoflavones, e.g. daidzein and genistein, by field-grown soybean. Isoflavone glucosides are secreted into the apoplast. Isoflavones in the rhizosphere soil were shown to induce the expression of rhizobial Nod genes, which initiate the formation of the nodules that fix nitrogen. In addition, isoflavones act as antimicrobial phytoalexins and modulate rhizosphere microbial communities
additional information
modeling of daidzein content in the rhizosphere
additional information
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modeling of daidzein content in the rhizosphere
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cloning of gene homologues GmIF7GT1-GmIF7GT6, DNA and amino acid sequence determination, expression analysis
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)
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gene GmIF7GT, DNA and amino acid sequence determination and analysis, expression analysis
gene GmIF7GT, recombinant functional expression in Escherichia coli strain BL21(DE3), coexpression with acyl-CoA carboxylase alpha and beta subunits, biotin ligase, and acetyl-CoA synthetase from Nocardia farcinia for subsequent malonylization of the reaction product of GmIF7GT-encoded isoflavone 7-O-glucosyltransferase. The isoflavonoids are glycosylated at position 7 by 7-O-glycosyltranferase and are further malonylated at position 6' of glucose by malonyl-CoA:isoflavone 7-O-glucoside-6'-O-malonyltransferase both from Glycine max
gene GmIF7GT1, quantitative real-time reverse transcription-PCR enzyme expression analysis
gene GmUGT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis
gene GmUGT3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis
gene GmUGT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis
gene GmUGT7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis
gene GmUGT9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis
gene PlUGT1 or UGT88E12, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli and in Saccharomyces cerevisiae strain WAT11
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glutathione S-transferase fusion protein expressed in Escherichia coli
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Koester, J.; Barz, W.
UDP-glucose:isoflavone 7-O-glucosyltransferase from roots of chick pea (Cicer arietinum L.)
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1981
Cicer arietinum
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Vellekoop, P.; Lugones, L.; van Brederode, J.
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Kim, J.H.; Kim, B.G.; Park, Y.; Ko, J.H.; Lim, C.E.; Lim, J.; Lim, Y.; Ahn, J.
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Arabidopsis thaliana (Q8VZE9), Arabidopsis thaliana
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A UDP-glucose:isoflavone 7-O-glucosyltransferase from the roots of soybean (Glycine max) seedlings: Purification, gene cloning, phylogenetics, and an implication for an alternative strategy of enzyme catalysis
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Four glucosyltransferases from rice: cDNA cloning, expression, and characterization
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Oryza sativa (Q7XHR3), Oryza sativa
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Cyclic GMP acts as a common regulator for the transcriptional activation of the flavonoid biosynthetic pathway in soybean
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2009
Glycine max
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Livingstone, J.M.; Zolotarov, Y.; Stroemvik, M.V.
Transcripts of soybean isoflavone 7-O-glucosyltransferase and hydroxyisoflavanone dehydratase gene homologues are at least as abundant as transcripts of their well known counterparts
Plant Physiol. Biochem.
49
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2011
Glycine max (A6BM07), Glycine max, Glycine max Williams 82 (A6BM07)
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An in silico study of the genes for the isoflavonoid pathway enzymes in soybean reveals novel expressed homologues
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90
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2010
Glycine max (A6BM07)
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Koirala, N.; Pandey, R.P.; Thang, D.V.; Jung, H.J.; Sohng, J.K.
Glycosylation and subsequent malonylation of isoflavonoids in E. coli: strain development, production and insights into future metabolic perspectives
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41
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2014
Glycine max (A6BM07)
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Funaki, A.; Waki, T.; Noguchi, A.; Kawai, Y.; Yamashita, S.; Takahashi, S.; Nakayama, T.
Identification of a highly specific isoflavone 7-O-glucosyltransferase in the soybean (Glycine max (L.) Merr.)
Plant Cell Physiol.
56
1512-1520
2015
Glycine max, Glycine max (A6BM07)
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Li, J.; Li, Z.; Li, C.; Gou, J.; Zhang, Y.
Molecular cloning and characterization of an isoflavone 7-O-glucosyltransferase from Pueraria lobata
Plant Cell Rep.
33
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2014
Pueraria montana var. lobata
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Sugiyama, A.; Yamazaki, Y.; Hamamoto, S.; Takase, H.; Yazaki, K.
Synthesis and secretion of isoflavones by field-grown soybean
Plant Cell Physiol.
58
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2017
Glycine max (A6BM07), Glycine max
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