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2-dehydro-D-glucose + alpha-D-glucose 1-phosphate
? + phosphate
-
-
-
-
?
2-deoxy-D-glucose + alpha-D-glucose 1-phosphate
?
-
8% of the activity with alpha-D-glucose
-
-
r
2-deoxy-D-glucose + alpha-D-glucose 1-phosphate
? + phosphate
-
-
-
-
?
2-fluoro-D-glucose + alpha-D-glucose 1-phosphate
?
-
15% of the activity with alpha-D-glucose
-
-
r
2-fluoro-D-glucose + alpha-D-glucose 1-phosphate
? + phosphate
-
-
-
-
?
2-keto-D-glucose + alpha-D-glucose 1-phosphate
?
-
50% of the activity with alpha-D-glucose
-
-
r
alpha,alpha-trehalose + arsenate
alpha-D-glucose + alpha-D-glucose 1-arsenate
-
-
-
-
r
alpha,alpha-trehalose + arsenate
alpha-D-glucose 1-arsenate + alpha-D-glucose
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
alpha-D-glucose 1-fluoride + phosphate
alpha-D-glucose 1-phosphate + fluoride
D-mannose + alpha-D-glucose 1-phosphate
?
-
3% of the activity with alpha-D-glucose
-
-
r
D-mannose + alpha-D-glucose 1-phosphate
? + phosphate
-
-
-
-
?
additional information
?
-
alpha,alpha-trehalose + arsenate
alpha-D-glucose 1-arsenate + alpha-D-glucose
-
30% of the activity with phosphate
-
-
?
alpha,alpha-trehalose + arsenate
alpha-D-glucose 1-arsenate + alpha-D-glucose
-
-
-
-
r
alpha,alpha-trehalose + arsenate
alpha-D-glucose 1-arsenate + alpha-D-glucose
-
-
alpha-D-glucose 1-arsenate is unstable, therefore the net reaction gives two mol of alpha-D-glucose
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
key role in trehalose metabolism
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
key role in trehalose metabolism
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
reverse reaction only utilizes the alpha-anomers of D-glucose-1-phosphate and D-glucose and gives exclusively the alpha,alpha-product
r
alpha-D-glucose + alpha-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
-
-
-
r
alpha-D-glucose + alpha-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
-
beta-D-glucose 1-phosphate is not turned over by the enzyme
-
-
r
alpha-D-glucose + alpha-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
-
-
-
r
alpha-D-glucose + alpha-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
ratio of phosphorolysis to hydrolysis is 46000
-
-
r
alpha-D-glucose + alpha-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
ratio of phosphorolysis to hydrolysis is 46000
-
-
r
alpha-D-glucose 1-fluoride + phosphate
alpha-D-glucose 1-phosphate + fluoride
-
-
-
-
?
alpha-D-glucose 1-fluoride + phosphate
alpha-D-glucose 1-phosphate + fluoride
-
poor substrate, alpha-D-glucose 1-fluoride is recognized as a substrate analogue of alpha,alpha-trehalose but not of alpha-D-glucose 1-phosphate
-
-
?
additional information
?
-
-
the enzyme is involved in trehalose catabolism
-
-
?
additional information
?
-
-
the maltose phosphorylase loop 3 variants TP2 and TP3 are capable of phosphorolysing trehalose (alpha, alpha1) as well as maltose (alpha-1,4),but show no activity towards kojibiose (alpha-1,2), nigerose (alpha-1,3) or isomaltose (alpha-1,6). The catalytic efficiency of TP3 towards trehalose is 19fold higher than of TP2
-
-
?
additional information
?
-
-
the maltose phosphorylase loop 3 variants TP2 and TP3 are capable of phosphorolysing trehalose (alpha, alpha1) as well as maltose (alpha-1,4),but show no activity towards kojibiose (alpha-1,2), nigerose (alpha-1,3) or isomaltose (alpha-1,6). The catalytic efficiency of TP3 towards trehalose is 19fold higher than of TP2
-
-
?
additional information
?
-
-
no measurable activity is found with D-arabinose, D-fructose, D-fucose, D-galactose, D-lyxose, D-mannoheptaose, D-ribose and D-xylose
-
-
?
additional information
?
-
during heat stress, trehalose phosphorylase shows reversible activity
-
-
-
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alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
additional information
?
-
-
the enzyme is involved in trehalose catabolism
-
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
key role in trehalose metabolism
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
key role in trehalose metabolism
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
r
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22 - 91
alpha,alpha-trehalose
0.63 - 505
alpha-D-glucose
5.9
alpha-D-glucose 1-fluoride
-
30°C, pH 6.6
1.6 - 47
alpha-D-glucose 1-phosphate
additional information
additional information
-
mutant K512A (phosphate): no saturation, Kcat/Km (phosphate): 0.0014/Msec
-
22
alpha,alpha-trehalose
-
loop 3 variant TP3, in 40 mM phosphate-citrate pH 6.2 at 45°C
53
alpha,alpha-trehalose
30°C, pH 6.6, recombinant enzyme
53
alpha,alpha-trehalose
-
loop 3 variant TP2, in 40 mM phosphate-citrate pH 6.2 at 45°C
61
alpha,alpha-trehalose
-
30°C, pH 7
71
alpha,alpha-trehalose
30°C, pH 6.6, natural enzyme
74.8
alpha,alpha-trehalose
pH 7
75
alpha,alpha-trehalose
-
-
75
alpha,alpha-trehalose
-
30°C, pH 7
79
alpha,alpha-trehalose
-
-
79
alpha,alpha-trehalose
-
30°C, pH 6.8
87
alpha,alpha-trehalose
-
30°C, pH 6.6
91
alpha,alpha-trehalose
-
30°C, pH 6.6
0.63
alpha-D-glucose
-
30°C, pH 6.3
24
alpha-D-glucose
-
30°C, pH 7
31
alpha-D-glucose
-
30°C, pH 6.6
34.3
alpha-D-glucose
pH 6.3
35
alpha-D-glucose
30°C, pH 6.6, natural enzyme
39
alpha-D-glucose
-
30°C, pH 6.6
40
alpha-D-glucose
-
30°C, pH 6.8
46
alpha-D-glucose
30°C, pH 6.6, recombinant enzyme
1.6
alpha-D-glucose 1-phosphate
30°C, pH 6.6, natural enzyme
2.2
alpha-D-glucose 1-phosphate
30°C, pH 6.6, recombinant enzyme
2.8
alpha-D-glucose 1-phosphate
-
30°C, pH 6.6
4.1
alpha-D-glucose 1-phosphate
-
-
4.1
alpha-D-glucose 1-phosphate
-
30°C, pH 6.8
6.3
alpha-D-glucose 1-phosphate
-
30°C, pH 7
38
alpha-D-glucose 1-phosphate
-
-
44.6
alpha-D-glucose 1-phosphate
pH 6.3
47
alpha-D-glucose 1-phosphate
-
30°C, pH 6.3
0.8
phosphate
30°C, pH 6.6, recombinant enzyme
0.8
phosphate
-
wild-type, Kcat/Km (phosphate): 21000/Msec
1.4
phosphate
30°C, pH 6.6, natural enzyme
2.4
phosphate
-
30°C, pH 6.6
3.5
phosphate
-
30°C, pH 6.8
4.7
phosphate
-
30°C, pH 7
103
phosphate
-
mutant R507A, Kcat/Km (phosphate): 0.0004/Msec
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Eis, C.; Albert, M.; Dax, K.; Nidetzky, B.
The stereochemical course of the reaction mechanism of trehalose phosphorylase from Schizophyllum commune
FEBS Lett.
440
440-443
1998
Schizophyllum commune
brenda
Eis, C.; Nidetzky, B.
Characterization of trehalose phosphorylase from Schizophyllum commune
Biochem. J.
341
385-393
1999
Schizophyllum commune
brenda
Eis, C.; Nidetzky, B.
Substrate-binding recognition and specificity of trehalose phosphorylase from Schizophyllum commune examined in steady-state kinetic studies with deoxy and deoxyfluoro substrate analogues and inhibitors
Biochem. J.
363
335-340
2002
Schizophyllum commune
brenda
Eis, C.; Watkins, M.; Prohaska, T.; Nidetzky, B.
Fungal trehalose phosphorylase: kinetic mechanism, pH-dependence of the reaction and some structural properties of the enzyme from Schizophyllum commune
Biochem. J.
356
757-767
2001
Schizophyllum commune
brenda
Han, S.E.; Kwon, H.B.; Lee, S.B.; Yi, B.Y.; Murayama, I.; Kitamoto, Y.; Byun, M.O.
Cloning and characterization of a gene encoding trehalose phosphorylase (TP) from Pleurotus sajor-caju
Protein Expr. Purif.
30
194-202
2003
Lentinus sajor-caju (Q9UV63)
brenda
Kitamoto, Y.; Akashi, H.; Tanaka, H.; Mori, N.
alpha-Glucose-1-phosphate formation by a novel trehalose phosphorylase from Flammulina velutipes
FEMS Microbiol. Lett.
55
147-149
1988
Flammulina velutipes
brenda
Kitamoto, Y.; Osaki, N.; Tanaka, H.; Sasaki, H.; Mori, N.
Purification and properties of alpha-glucose 1-phosphate-forming trehalose phosphorylase from a basidiomycete, Pleurotus ostreatus
Mycoscience
41
607-613
2000
Pleurotus ostreatus
-
brenda
Kitamoto, Y.; Tanaka, H.; Osaki, N.
Survey of alpha-glucose 1-phosphate forming trehalose phosphorylase and trehalase in various fungi including basidiomycetous mushrooms
Mycoscience
39
327-331
1998
Agaricus sp., Polyporales
-
brenda
Nidetzky, B.; Eis, C.
alpha-Retaining glucosyl transfer catalysed by trehalose phosphorylase from Schizophyllum commune: mechanistic evidence obtained from steady-state kinetic studies with substrate analogues and inhibitors
Biochem. J.
360
727-736
2001
Schizophyllum commune
brenda
Saito, K.; Kase, T.; Takahashi, E.; Horinouchi, S.
Purification and characterization of a trehalose synthase from the basidiomycete Grifola frondosa
Appl. Environ. Microbiol.
64
4340-4345
1998
Grifola frondosa
brenda
Wannet, W.J.; Op den Camp, H.J.; Wisselink, H.W.; van der Drift, C.; Van Griensven, L.J.; Vogels, G.D.
Purification and characterization of trehalose phosphorylase from the commercial mushroom Agaricus bisporus
Biochim. Biophys. Acta
1425
177-188
1998
Agaricus bisporus, Agaricus bisporus Horst U1
brenda
Dmitryjuk, M.; Zoltowska, K.
Trehalose catabolism enzymes in tissues of Ascaris suum (Nematoda)
Helminthologia
41
63-66
2004
Ascaris suum
-
brenda
Goedl, C.; Griessler, R.; Schwarz, A.; Nidetzky, B.
Structure-function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferases
Biochem. J.
397
491-500
2006
Schizophyllum commune (Q2HZZ3), Schizophyllum commune BT 2115 (Q2HZZ3)
brenda
Schwarz, A.; Goedl, C.; Minani, A.; Nidetzky, B.
Trehalose phosphorylase from Pleurotus ostreatus: characterization and stabilization by covalent modification, and application for the synthesis of alpha,alpha-trehalose
J. Biotechnol.
129
140-150
2007
Pleurotus ostreatus
brenda
Brecker, L.; Schwarz, A.; Goedl, C.; Kratzer, R.; Tyl, C.E.; Nidetzky, B.
Studying non-covalent enzyme carbohydrate interactions by STD NMR
Carbohydr. Res.
343
2153-2161
2008
Schizophyllum commune
brenda
Goedl, C.; Nidetzky, B.
The phosphate site of trehalose phosphorylase from Schizophyllum commune probed by site-directed mutagenesis and chemical rescue studies
FEBS J.
275
903-913
2008
Schizophyllum commune
brenda
Nakai, H.; Petersen, B.O.; Westphal, Y.; Dilokpimol, A.; Abou Hachem, M.; Duus, J.?.; Schols, H.A.; Svensson, B.
Rational engineering of Lactobacillus acidophilus NCFM maltose phosphorylase into either trehalose or kojibiose dual specificity phosphorylase
Protein Eng. Des. Sel.
23
781-787
2010
Lactobacillus acidophilus, Lactobacillus acidophilus NCFM
brenda
Liu, X.; Wu, X.; Gao, W.; Qu, J.; Chen, Q.; Huang, C.; Zhang, J.
Protective roles of trehalose in Pleurotus pulmonarius during heat stress response
J. Integr. Agric.
18
428-437
2019
Pleurotus pulmonarius (A6YRN9)
-
brenda
Liu, J.H.; Shang, X.D.; Liu, J.Y.; Tan, Q.
Changes in trehalose content, enzyme activity and gene expression related to trehalose metabolism in Flammulina velutipes under heat shock
Microbiology
162
1274-1285
2016
Flammulina velutipes (A0A0G2SWS6)
brenda
Zhao, X.; Song, X.; Li, Y.; Yu, C.; Zhao, Y.; Gong, M.; Shen, X.; Chen, M.
Gene expression related to trehalose metabolism and its effect on Volvariella volvacea under low temperature stress
Sci. Rep.
8
11011
2018
Volvariella volvacea
brenda