Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-galactose +
?
-
-
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-Glc(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
95-100% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
90-95% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc-N3(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc6N3(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc6NH2(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
20% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc6OSO3-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
100% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAcPh(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAcPhPh(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
100% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNGc(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNTFA(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNTFA-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
60-65% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl-beta-D-xylopyranoside
?
-
-
-
?
UDP-alpha-D-galactose + asialo-agalacto-transferrin
?
-
-
-
-
?
UDP-alpha-D-galactose + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-(N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-)mannosyl-beta-1,4-N-acetylglucosaminyl-beta-1,4-(fucosyl-alpha-1,6-)N-acetylglucosaminyl-asparagine
?
-
-
-
-
?
UDP-alpha-D-galactose + Glc-ITag
UDP + Lac-ITag
UDP-alpha-D-galactose + GlcNac-ITag
UDP + LacNAc-ITag
UDP-alpha-D-galactose + glycopeptide from porcine immunoglobulin G
?
-
-
-
-
?
UDP-alpha-D-galactose + immunoglobulin G heavy chain
?
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
UDP-alpha-D-galactose + N-acetylgalactosamine
?
-
-
-
-
?
UDP-alpha-D-galactose + N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-(galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-)mannosyl-beta-1,4-N-acetylglucosaminyl-beta-1,4-(fucosyl-alpha-1,6-)N-acetylglucosaminyl-asparagine
?
-
-
-
-
?
UDP-Gal + 4-nitrophenyl 2-acetamido-2-deoxy-4-thio-beta-D-glucopyranoside
UDP + Gal-beta-S-1,4-GlcNAcpNP
-
HP0826 catalyzes the synthesis of the thiodisaccharide Gal-beta-S-1,4-GlcNAcpNP as well as Gal-beta-1,4-Man-pNP with total regio- and stereoselectivity, overview
-
-
?
UDP-Gal + 4-nitrophenyl beta-D-mannopyranoside
UDP + Gal-beta-1,4-Man-pNP
UDP-Gal + 4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside
UDP + ?
-
-
-
-
?
UDP-galactose + 3-deoxy-3-fluoro-GlcNAcbeta-Bn
UDP + Galbeta(1-4)3-deoxy-3-fluoro-GlcNAcbeta-Bn
-
-
-
-
?
UDP-galactose + 4-methylumbelliferyl beta-D-xylopyranoside
UDP + ?
-
-
-
-
?
UDP-galactose + 4-nitrophenyl beta-D-xylopyranoside
UDP + beta-D-galactopyranosyl-1,4-xylopyranosyl-1-O-4-nitrophenol
-
-
-
-
?
UDP-galactose + 6-deoxy-GlcNAcbeta-Bn
UDP + Galbeta(1-4)6-deoxy-GlcNAcbeta-Bn
-
low activity
-
-
?
UDP-galactose + 6-thio-GlcNAcbeta-Bn
UDP + Galbeta(1-4)6-thio-GlcNAcbeta-Bn
-
low activity
-
-
?
UDP-galactose + benzyl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
UDP + benzyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
UDP-galactose + chitotriose
?
GlcNAcbeta1,4-GlcNAcbeta1,4-GlcNAc
-
-
?
UDP-galactose + colchicoside
UDP + beta-D-galactosyl-1,4-colchicoside
-
-
-
-
?
UDP-galactose + ginsenoside Rg1
UDP + beta-D-galactosyl-1,4-ginsenoside Rg1
-
-
-
-
?
UDP-galactose + Glc-NAcbeta1-3Galbeta-O-naphthalenemethanol
?
-
-
-
?
UDP-galactose + GlcNAcbeta-Bn
UDP + Galbeta(1-4)GlcNAcbeta-Bn
-
-
-
-
?
UDP-galactose + GlcNAcbeta1,2-Manalpha1,3-Manbeta-OR
?
an N-linked biantennary oligosaccharide chain, one antenna is attached to the 3-hydroxyl-(1,2-1,3-arm) group of mannose, which is beta-1,4-linked to an N-linked chitobiose, attached to the aspargine residue of a protein
-
-
?
UDP-galactose + GlcNAcbeta1,2-Manalpha1,6 (GlcNAcbeta1,2-Manalpha1,3)Man
?
-
-
-
?
UDP-galactose + GlcNAcbeta1,2-Manalpha1,6-Manbeta-OR
?
an N-linked biantennary oligosaccharide chain, one antenna is attached to the 3-hydroxyl-(1,2-1,6-arm) group of mannose, which is beta-1,4-linked to an N-linked chitobiose, attached to the aspargine residue of a protein
-
-
?
UDP-galactose + GlcNAcbeta1,4-Manalpha1,3-Manbeta-OR
?
an N-linked biantennary oligosaccharide chain, one antenna is attached to the 3-hydroxyl-(1,4-1,3-arm) group of mannose, which is beta-1,4-linked to an N-linked chitobiose, attached to the aspargine residue of a protein
-
-
?
UDP-galactose + lactose
UDP + beta-D-galactosyl-lactose
-
-
-
-
?
UDP-galactose + laminin
UDP + beta-1,4-D-galactosyl-laminin
-
-
-
-
?
UDP-galactose + methyl 2-acetamido-2-deoxy-beta-D-glucoside
UDP + beta-D-galactosyl-1,4-beta-1-O-methyl-2-deoxy-2-acetylamido-beta-D-glucopyranoside
-
76% of the activity with N-acetylglucosamine
-
-
?
UDP-galactose + methyl 2-bromo-acetamido-2-deoxy-beta-D-glucoside
UDP + beta-D-galactosyl-1,4-1-O-methyl-2-bromo-acetamido-2-deoxy-beta-D-glucoside
-
75% of the activity with N-acetylglucosamine
-
-
?
UDP-galactose + methyl 2-deoxy-2-benzamido-beta-D-glucoside
UDP + beta-D-galactosyl-1,4-1-O-methyl-2-deoxy-2-benzamido-beta-D-glucoside
-
16% of the activity with N-acetylglucosamine
-
-
?
UDP-galactose + N-4-toluenesulfonyl-GlcN
UDP + Galbeta(1-4)N-4-toluenesulfonyl-GlcN
-
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosamine
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminyl-1,4-N-acetyl-beta-D-glucosamine
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,4-N-acetyl-beta-D-glucosamine
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1-4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
for the binding of GlcNAc in the monosaccharide binding site of beta4Gal-T1, there is a hydrophobic N-acetyl group-binding pocket formed by residues Arg359, Phe360, and Ile363 from the ?-helix region in the closed conformation of the long loop, the sugar donor specificity of beta4Gal-T1 toward galactose is determined by the Tyr residue at position 289 in the binding pocket. The two proteins beta4Gal-T1 and alpha-lactalbukin crystallize together as a complex only in the presence of either donor substrate or acceptor Glc or GlcNAc to form lactose synthase. The specificity of the sugar donor is generally determined by a few residues in the sugar-nucleotide binding pocket. The conformational change in beta4Gal-T1 also creates the binding site for a mammary gland-specific protein, alpha-lactalbumin, which changes the acceptor specificity of the enzyme toward glucose to synthesize lactose during lactation
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
UDP-galactose + N-acetylglucosamine
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
UDP-galactose + N-butyryl-GlcNbeta-Bn
UDP + Galbeta(1-4)N-butyryl-GlcNbeta-Bn
-
-
-
-
?
UDP-galactose + N-methanesulfonyl-GlcN
UDP + Galbeta(1-4)N-methanesulfonyl-GlcN
-
-
-
-
?
UDP-galactose + N-trifluoroacetyl-GlcN
UDP + Galbeta(1-4)N-trifluoroacetyl-GlcN
-
-
-
-
?
UDP-galactose + p-nitrophenyl-beta-D-N-acetylglucosaminide
?
UDP-galactose + T(GlcNAcbeta3GalNAcbeta)TTVTPTPTG
?
-
-
-
-
?
UDP-galactose + T(GlcNAcbeta6GalNAcbeta)TTVTPTPTG
?
-
-
-
-
?
UDP-galactose + T(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TTVTPTPTG
?
-
-
-
-
?
UDP-galactose + T-(GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TTVTPTPTG
UDP + T-(Galbeta1-4GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TTVTPTPTG
-
pH 7.0, 0.5 mM, 40% activity
-
-
?
UDP-galactose + TT(GlcNAcbeta3GalNAcbeta)TVTPTPTG
?
-
-
-
-
?
UDP-galactose + TT(GlcNAcbeta6GalNAcbeta)TVTPTPTG
?
-
-
-
-
?
UDP-galactose + TT(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TVTPTPTG
?
-
-
-
-
?
UDP-galactose + TT-(GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TVTP(GGA)TPTG
UDP + TT-(Galbeta1-4GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TVTP(GGA)TPTG
-
pH 7.0, 0.5 mM, 25% activity
-
-
?
UDP-galactose + TT-(GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TVTPTPTG
UDP + TT-(Galbeta1-4GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TVTPTPTG
UDP-galactose + TTTV (GlcNAcbeta3GalNAcbeta)TPTPTG
?
-
-
-
-
?
UDP-galactose + TTTV(GlcNAcbeta6GalNAcbeta)TPTPTG
?
-
-
-
-
?
UDP-galactose + TTTV(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TPTPTG
?
-
-
-
-
?
UDP-galactose + TTTVTP(GlcNAcbeta3GalNAcbeta)TPTG
?
-
-
-
-
?
UDP-galactose + TTTVTP(GlcNAcbeta6GalNAcbeta)TPTG
?
-
-
-
-
?
UDP-galactose + TTTVTP-(GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TPTG
UDP + TTTVTP-(Galbeta1-4GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TPTG
-
pH 7.0, 0.5 mM, 20% activity
-
-
?
UDP-galactose + TTTVTP-(GlcNAcbeta1-6[Galbeta1-3]GalNAcalpha1-)TG
UDP + TTTVTP-(Galbeta-1-4GlcNAcbeta1-6[Galbeta1-3]GalNAcalpha1-)TG
pH 7.0, 0.2 mM, 2% activity in A549 cells, 14% activity in H292 cells relative to benzyl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
-
-
?
UDP-galactose + TTTVTPTP(GlcNAcbeta3GalNAcbeta)TG
?
-
-
-
-
?
UDP-galactose + TTTVTPTP(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TG
?
-
-
-
-
?
UDP-glucose + 4-methylumbelliferyl beta-D-xylopyranoside
UDP + 4-O-beta-D-galactosyl-beta-D-glucose
-
1.5% activity with UDO-glucose compared to UDP-galactose
-
-
?
UDP-glucose + N-acetylglucosamine
UDP + beta-D-glucosyl-1,4-N-acetyl-beta-D-glucosamine
-
reaction with 0.3% efficiency. R228K mutation results in a 15-fold higher glucosyltransferase activity, which is further enhanced by alpha-lactalbumin to nearly 25% of the galactosyltransferase activity of the wild type
-
-
?
UDP-glucuronic acid + 4-methylumbelliferyl beta-D-xylopyranoside
UDP + ?
-
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + 4-methylumbelliferyl beta-D-xylopyranoside
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + 4-methylumbelliferyl beta-D-xylopyranoside
UDP + ?
-
-
-
-
?
UDP-xylose + 4-methylumbelliferyl beta-D-xylopyranoside
UDP + ?
-
-
-
-
?
UDPgalactose + 2-acetamido-N-(L-aspart-4-oyl)-1,2-dideoxy-beta-glucoside
?
-
65% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + 3-acetamido-3-deoxy-D-xylose
?
-
-
-
-
?
UDPgalactose + agalacto-ovomucoid
?
-
65% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + agalacto-poly-N-acetyllactosamine
?
-
-
-
-
?
UDPgalactose + agalactokeratan
?
-
agalactokeratan from bovine cornea and nasal septum, at 5% and 13% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + alpha1-acid glycoprotein
?
-
-
-
-
?
UDPgalactose + asialo agalacto alpha1 acid glycoprotein
?
-
-
-
-
?
UDPgalactose + asialo-agalacto-alpha1-glycoprotein
?
-
42% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + asialo-agalacto-transferrin
?
-
transfer of galactose to N-acetylglucosamine residues of Asn-linked sugar chains of glycoproteins in a beta1-4linkage
-
-
?
UDPgalactose + asialogalactofetuin
?
-
-
-
-
?
UDPgalactose + chitobiose
?
-
-
-
-
?
UDPgalactose + chitotriose
?
-
-
-
-
?
UDPgalactose + degalactosylated fetuin
UDP + fetuin containing beta-1,4-galactose linkages
UDPgalactose + di-acetylchitobiose
?
-
54% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + fetuin
?
-
-
-
-
?
UDPgalactose + GlcNAcbeta-S-p-NP
UDP + Galbeta1-4 GlcNAcbeta-S-p-NP
-
-
-
?
UDPgalactose + GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-3Manbeta1-O(CH2)8COOCH2-mNP
UDP + Galbeta1-4 GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-3Manbeta1-O(CH2)8COOCH2-mNP
-
-
-
?
UDPgalactose + glucose
lactose + UDP
UDPgalactose + immunoglobulin heavy chain
?
-
-
-
-
?
UDPgalactose + lacto-N-triaosylceramide
?
-
-
-
-
?
UDPgalactose + lacto-N-triose II
?
-
-
-
-
?
UDPgalactose + N-acetamido-3-deoxy-D-glucose
?
-
-
-
-
?
UDPgalactose + N-acetyl-beta-D-glucosaminyl-glycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
UDPgalactose + N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-(N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-)mannosyl-beta-1,4-N-acetylglucosaminyl-beta-1,4-(fucosyl-alpha-1,6-)N-acetylglucosaminyl-asparagine
UDP + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-(galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-)mannosyl-beta-1,4-N-acetylglucosaminyl-beta-1,4-(fucosyl-alpha-1,6-)N-acetylglucosaminyl-asparagine
-
-
galactose is transferred much faster to the N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-branch than to the N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-branch
?
UDPgalactose + N-acetylglucosaminyl-beta-1,3-(galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,6-)galactose
UDP + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,3-(galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,6-)galactose
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl-beta-1,3-(N-acetylglucosaminyl-beta-1,6-)galactose
UDP + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,3-(N-acetylglucosaminyl-beta-1,6-)galactose
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl-beta-1,3-galactose
UDP + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,3-galactose
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl-beta-1,6-galactose
UDP + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,6-galactose
-
-
-
-
?
UDPgalactose + ovalbumin
?
UDPgalactose + ovomucoid
UDP + ovomucoid with beta-1,4-bound galactose
-
-
-
?
UDPgalactose + p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
?
UDPgalactose + tri-N-acetylchitotriose
?
-
64% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + UDPglucose
UDP + lactose
-
-
-
?
additional information
?
-
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
100% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
100% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc-N3(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
35-40% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc-N3(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
100% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc-N3(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
100% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc6N3(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
100% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAc6N3(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
5-10% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAcPh(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
20-25% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAcPh(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
50% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNAcPh(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
100% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNGc(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
75-80% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNGc(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
90-95% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNTFA(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
100% activity
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-GlcNTFA(1->3)-beta-D-Gal-(1->4)-beta-D-Glc
?
-
95-100% activity
-
-
?
UDP-alpha-D-galactose + Glc-ITag
UDP + Lac-ITag
Glc-ITag is 3-(4-(3-methylimidazolium)methylbenzyloxycarbonylamino)propyl-beta-D-glucopyranoside
-
-
?
UDP-alpha-D-galactose + Glc-ITag
UDP + Lac-ITag
Glc-ITag is 3-(4-(3-methylimidazolium)methylbenzyloxycarbonylamino)propyl-beta-D-glucopyranoside
-
-
?
UDP-alpha-D-galactose + Glc-ITag
UDP + Lac-ITag
Neisseria meningitidis serogroup A Z2491
Glc-ITag is 3-(4-(3-methylimidazolium)methylbenzyloxycarbonylamino)propyl-beta-D-glucopyranoside
-
-
?
UDP-alpha-D-galactose + Glc-ITag
UDP + Lac-ITag
Glc-ITag is 3-(4-(3-methylimidazolium)methylbenzyloxycarbonylamino)propyl-beta-D-glucopyranoside
-
-
?
UDP-alpha-D-galactose + Glc-ITag
UDP + Lac-ITag
Glc-ITag is 3-(4-(3-methylimidazolium)methylbenzyloxycarbonylamino)propyl-beta-D-glucopyranoside
-
-
?
UDP-alpha-D-galactose + GlcNac-ITag
UDP + LacNAc-ITag
GlcNac-ITag is 3-(4-(3-methylimidazolium)methylbenzylcarbamate)propyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
-
?
UDP-alpha-D-galactose + GlcNac-ITag
UDP + LacNAc-ITag
GlcNac-ITag is 3-(4-(3-methylimidazolium)methylbenzylcarbamate)propyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
-
?
UDP-alpha-D-galactose + GlcNac-ITag
UDP + LacNAc-ITag
Neisseria meningitidis serogroup A Z2491
GlcNac-ITag is 3-(4-(3-methylimidazolium)methylbenzylcarbamate)propyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
-
?
UDP-alpha-D-galactose + GlcNac-ITag
UDP + LacNAc-ITag
GlcNac-ITag is 3-(4-(3-methylimidazolium)methylbenzylcarbamate)propyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
-
?
UDP-alpha-D-galactose + GlcNac-ITag
UDP + LacNAc-ITag
GlcNac-ITag is 3-(4-(3-methylimidazolium)methylbenzylcarbamate)propyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
Neisseria meningitidis serogroup A Z2491
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-Gal + 4-nitrophenyl beta-D-mannopyranoside
UDP + Gal-beta-1,4-Man-pNP
-
HP0826 catalyzes the synthesis of the thiodisaccharide Gal-beta-S-1,4-GlcNAcpNP as well as Gal-beta-1,4-Man-pNP with total regio- and stereoselectivity, overview
-
-
?
UDP-Gal + 4-nitrophenyl beta-D-mannopyranoside
UDP + Gal-beta-1,4-Man-pNP
-
LgtB catalyzes the synthesis of Gal-beta-1,4-Man-pNP, overview
-
-
?
UDP-galactose + benzyl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
UDP + benzyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
pH 7.0, 0.5 mM, 100% activity
-
-
?
UDP-galactose + benzyl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
UDP + benzyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
pH 7.0
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
catalytic cycle: Mn2+ binds first before UDP-Gal, two flexible loops, a long and a short loop, change their conformation from open to closed
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
the enzyme effectively galactosylates the GlcNAcbeta1,6Man arm of the highly branched N-glycans that are characteristic of glioma, overview
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
the enzyme transfers galactose from UDP-galactose to N-acetylglucosamine residues of the branched N-linked oligosaccharide chains of glycoproteins
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
catalytic cycle: Mn2+ binds first before UDP-Gal, two flexible loops, a long and a short loop, change their conformation from open to closed
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
residue Y285 alone is responsible for determination of enzyme substrate specificity, overview, active site structure, overview
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
UDP-Gal-Mn2+ substrate binding structure, overview
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
100% activity with UDP-galactose
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + p-nitrophenyl-beta-D-N-acetylglucosaminide
?
-
-
-
-
?
UDP-galactose + p-nitrophenyl-beta-D-N-acetylglucosaminide
?
-
-
-
?
UDP-galactose + p-nitrophenyl-beta-D-N-acetylglucosaminide
?
-
-
-
?
UDP-galactose + TT-(GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TVTPTPTG
UDP + TT-(Galbeta1-4GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TVTPTPTG
-
pH 7.0, 0.5 mM, 30% activity
-
-
?
UDP-galactose + TT-(GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TVTPTPTG
UDP + TT-(Galbeta1-4GlcNAcbeta-1-6[Galbeta1-3]GalNAcalpha1-)TVTPTPTG
-
pH 7.0, 0.5 mM, 35% activity
-
-
?
UDP-GalNAc + GlcNAc
?
-
at high concentrations of alpha-lactalbumin
-
-
?
UDP-GalNAc + GlcNAc
?
-
transfer of GalNAc is only 1% of galactose transfer in wild type enzyme. Mutant enzyme Y289L exhibits nearly 100% of the galactose transferase activity
-
-
?
UDPgalactose + degalactosylated fetuin
UDP + fetuin containing beta-1,4-galactose linkages
-
-
-
?
UDPgalactose + degalactosylated fetuin
UDP + fetuin containing beta-1,4-galactose linkages
-
-
-
-
?
UDPgalactose + degalactosylated fetuin
UDP + fetuin containing beta-1,4-galactose linkages
-
-
-
-
?
UDPgalactose + degalactosylated fetuin
UDP + fetuin containing beta-1,4-galactose linkages
-
-
-
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
-
?
UDPgalactose + glucose
lactose + UDP
enzyme has no lactose synthase activity in presence of alpha-lactalbumin
-
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + N-acetyl-beta-D-glucosaminyl-glycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
glycoproteins containing terminal nonreducing N-acetylglucosaminyl units
-
-
?
UDPgalactose + N-acetyl-beta-D-glucosaminyl-glycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
glycopeptide prepared from porcine IgG immunoglobulin
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
7% of the activity with GlcNAcbeta-S-pNP
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme facilitates sperm binding to the oocyte zona pellucida
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
enzyme participates in the biosynthesis of the oligosaccharide structures of glycoproteins and glycolipids
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
enzyme functions in the coordinate biosynthesis of complex oligosaccharides, proposed to function in intercellular recognition and/or adhesion
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
biosynthesis of keratan sulfate-like polysaccharides
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme may be involved in the synthesis of poly-N-acetyllactosamine, lacto-N-neotetraose and probably lacto-N-neotetraosylceramide in addition to the formation of the Galbeta1-4GlcNAc group of glycoprotein sugar chains and lactose
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
main enzyme responsible for the transfer of galactose residues from UDPgalactose into terminal N-acetylglucosamine residues of complex-type oligosaccharides in newly synthesized glycoproteins in the Golgi apparatus. Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId, a severe neurologic disease characterized by a hydrocephalus, myopathy and blood-clotting defects
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme is involved in the biosynthesis of a variety of carbohydrate structures in glycoproteins and glycolipids
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
biosynthesis of carbohydrate moieties of glycoproteins and glycolipids, role in intercellular recognition and adhesion
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the soluble enzyme form from the luminal fluid of the epididymis is suggested to play a role on sperm maturation
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme may be involved in the synthesis of plasma glycoproteins by the liver during secretion, and may possibly be required for secretion of these proteins
-
-
?
UDPgalactose + ovalbumin
?
-
-
-
-
?
UDPgalactose + ovalbumin
?
-
-
-
-
?
UDPgalactose + ovalbumin
?
-
-
-
-
?
UDPgalactose + p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
?
-
67% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
?
-
-
-
-
?
additional information
?
-
-
regioselectivity towards specific C(4)glucose OH group in the complex protopanaxadiol glycoside ginsenoside Rb1
-
-
?
additional information
?
-
-
enzyme also catalyzes unusual galactosyl transfer to the 3-OH position of L-sugars
-
-
?
additional information
?
-
-
enzyme also catalyzes transfer of glucose from UDPglucose to N-acetylglucosamine
-
-
?
additional information
?
-
-
substrate specificity in presence or absence of alpha-lactalbumin, overview, the 'specifier' protein alpha-lactalbumin, which interacts with beta-1,4-GalT forming the lactose synthase complex, EC 2.4.1.2 and changing the substrate specificity to the reaction of EC 2.4.1.90, alpha-lactalbumin is not necessary when the acceptors are different glucopyranosides and, in some cases, it can even have an inhibitory effect, e.g. in reaction with the complex glucosides ginsenoside Rg1 and colchicoside, overview, in absence of alpha-lactalbumin, the enzyme is not active with glucose as acceptor
-
-
?
additional information
?
-
-
the enzyme catalyzes the transfer of Gal from UDP-Gal to GlcNAc-terminating acceptors with inversion of configuration of the glycosidic linkage, in presence of beta-lactalbumin, the enzyme changes its specificity to become lactose synthase, EC 2.4.1.22, and transfers Gal to glucose to synthesize lactose, substrate specificity, e.g. with GlcNAc-terminating O-glycopeptides, no activity with N-trimethylacetyl-GlcN, N,N-dimethyl-GlcN, N-isopropyl-GlcN, and N-(4-MeOBn)GlcN, overview
-
-
?
additional information
?
-
-
no activity with Glcbeta(1->3)Lacbeta-MU, GlcNAc6Sbeta(1->3)Lacbeta-MU, GlcNAc6N3beta(1->3)Lacbeta-MU, GlcNAc6NH2beta(1->3)Lacbeta-MU, GlcNAcPhPhbeta(1->3)Lacbeta-MU, GlcNGcbeta(1->3)Lacbeta-MU, GlcNH2beta(1->3)Lacbeta-MU, GalNAcbeta(1->3)Lacbeta-MU or Galbeta(1->)3Lacbeta-MU as acceptor
-
-
-
additional information
?
-
the enzyme is part of the endoplasmic reticulum and Golgi glycosylation network, glycosyltransferase metabolism, overview
-
-
?
additional information
?
-
-
an enzyme orthologue involved in the synthesis of glycoproteins and glycolipids, the sugar donor specificity of the enzyme toward galactose is determined by the Ile residue at position 289 in the binding pocket. The two proteins beta4Gal-T1 and alpha-lactalbukin crystallize together as a complex only in the presence of either donor substrate or acceptor Glc or GlcNAc to form lactose synthase. The specificity of the sugar donor is generally determined by a few residues in the sugar-nucleotide binding pocket. The conformational change in beta4Gal-T1 also creates the binding site for a mammary gland-specific protein, alpha-lactalbumin, which changes the acceptor specificity of the enzyme toward glucose to synthesize lactose during lactation
-
-
?
additional information
?
-
-
HP0826 is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori
-
-
?
additional information
?
-
-
HP0826 exhibits a pronounced acceptor promiscuity with the most striking result being the successful transfer of Gal to the 4-SH of pNP-4-S-beta-GlcNAc
-
-
?
additional information
?
-
-
no activity with Glcbeta(1->3)Lacbeta-MU, GlcNAcPhPhbeta(1->3)Lacbeta-MU, GlcNH2beta(1->3)Lacbeta-MU, GalNAcbeta(1->3)Lacbeta-MU or Galbeta(1->)3Lacbeta-MU as acceptor
-
-
-
additional information
?
-
-
enzyme activity might be involved in apoptotic pathway in hepatocarcinoma cells
-
-
?
additional information
?
-
-
decreasing the expression of GalT V in glioma cells promotes apoptosis, inhibits the invasion and migration and the ability of tumor formation in vivo, and reduces the activation of AKT, the enzyme functions as a glioma growth activator, overview, contribution of GalT V in the activation of AKT and MAPK kinase, contribution of serum or EGF in the transcription regulation of the GalT V gene, overview
-
-
?
additional information
?
-
-
downregulation of the enzyme inhibits CDK11(p58)-mediated apoptosis induced by cycloheximide, while ectopically expressed beta1,4-GT 1 increases CDK11p58-mediated apoptosis
-
-
?
additional information
?
-
-
the cell surface enzyme promotes apoptosis by inhibiting the epidermal growth factor receptor pathway via inhibition of the autophosphorylation of epidermal growth factor receptor especially at Try 1068, mechanism, overview
-
-
?
additional information
?
-
-
tumor beta-1,4-galactosyltransferase IV overexpression is closely associated with colorectal cancer metastasis and poor prognosis, relationships between tumor beta-1,4-GT-IV overexpression and clinicopathologic characteristics, overview
-
-
?
additional information
?
-
-
beta-1,4-GTs are a family of glycosyltransferases responsible for biosynthesizing N-acetyllactosamine by the transfer of a galactose from UDP-galactose to the terminal N-acetylgluosamine of acceptor sugars in glycoproteins or glycolipids with a beta-1,4-linkage
-
-
?
additional information
?
-
complex penta-antennary N-glycan structure, detailed substrate binding structure analysis of the wild-type and mutant M340H enzymes, overview
-
-
?
additional information
?
-
-
substrate specificity of wild-type and mutant Y289L enzymes, overview
-
-
?
additional information
?
-
-
beta 1,4GalT V galactosylates the beta1,6-GlcNAc branch of N-glycans at the cell surface and of secreted glycoproteins, and functions as a positive regulator in glioma development, overview. As2O3-induced gioma cell apoptosis mechanism involves beta 1,4GalT V inhibition, overview
-
-
?
additional information
?
-
-
beta-1,4-galactosylransferase participates in the synthesis of Galbeta1-4-GlcNac disaccharide unit of glycoconjugates, overview
-
-
?
additional information
?
-
-
the specific inhibitors of the phosphatidylinositol 3-kinase LY294002 and wortmannin up-regulate beta1,4-galactosyltransferase I and thus sensitize SMMC-7721 human hepatocarcinoma cells to cycloheximide-induced apoptosis, overview
-
-
?
additional information
?
-
-
beta4Gal-T1 participates in the synthesis of Galbeta1-4-GlcNac disaccharide unit of glycoconjugates
-
-
?
additional information
?
-
-
UDP-mannose and UDP-N-acetyl galactosamine are no substrates for soluble beta1,4-GalT7
-
-
?
additional information
?
-
-
LgtB is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori
-
-
?
additional information
?
-
-
no activity with GlcNAc6Sbeta(1->3)Lacbeta-MU, GlcNAc6NH2beta(1->3)Lacbeta-MU, GlcNH2beta(1->3)Lacbeta-MU, GalNAcbeta(1->3)Lacbeta-MU or Galbeta(1->)3Lacbeta-MU as acceptor
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of carbohydrate composition of milk during lactation, endocrine enzyme regulation, overview
-
-
?
additional information
?
-
-
beta-1,4-GalT I is a type II membrane-bound glycoprotein, which occurs the plasma membrane of different types of cells where it serves as a cell surface adhesion molecule mediating various cell-cell and cell-matrix interactions such as sperm-egg binding, cell spreading, migration, and neurite outgrowth
-
-
?
additional information
?
-
beta-1,4-GalT I is a type II membrane-bound glycoprotein, which occurs the plasma membrane of different types of cells where it serves as a cell surface adhesion molecule mediating various cell-cell and cell-matrix interactions such as sperm-egg binding, cell spreading, migration, and neurite outgrowth
-
-
?
additional information
?
-
-
beta-1,4-GalT-I plays a key role in the synthesis of selectin ligands such as sialy Lewis, sLex, and sulfated sLex, overview. beta-1,4-GalT-I may play an important role in the inflammation reaction
-
-
?
additional information
?
-
-
beta-1,4-GalT-I transfers galactose from UDP-galactose donors to terminal N-acetylglucosamine of carbohydrate chains in beta-1,4-linkage to form a Galbeta1->4GlcNAc structure
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
UDP-galactose + laminin
UDP + beta-1,4-D-galactosyl-laminin
-
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
additional information
?
-
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
Neisseria meningitidis serogroup A Z2491
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
-
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
the enzyme effectively galactosylates the GlcNAcbeta1,6Man arm of the highly branched N-glycans that are characteristic of glioma, overview
-
-
?
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
the enzyme transfers galactose from UDP-galactose to N-acetylglucosamine residues of the branched N-linked oligosaccharide chains of glycoproteins
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme facilitates sperm binding to the oocyte zona pellucida
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
enzyme participates in the biosynthesis of the oligosaccharide structures of glycoproteins and glycolipids
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
enzyme functions in the coordinate biosynthesis of complex oligosaccharides, proposed to function in intercellular recognition and/or adhesion
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
biosynthesis of keratan sulfate-like polysaccharides
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme may be involved in the synthesis of poly-N-acetyllactosamine, lacto-N-neotetraose and probably lacto-N-neotetraosylceramide in addition to the formation of the Galbeta1-4GlcNAc group of glycoprotein sugar chains and lactose
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
main enzyme responsible for the transfer of galactose residues from UDPgalactose into terminal N-acetylglucosamine residues of complex-type oligosaccharides in newly synthesized glycoproteins in the Golgi apparatus. Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId, a severe neurologic disease characterized by a hydrocephalus, myopathy and blood-clotting defects
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme is involved in the biosynthesis of a variety of carbohydrate structures in glycoproteins and glycolipids
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
biosynthesis of carbohydrate moieties of glycoproteins and glycolipids, role in intercellular recognition and adhesion
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the soluble enzyme form from the luminal fluid of the epididymis is suggested to play a role on sperm maturation
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme may be involved in the synthesis of plasma glycoproteins by the liver during secretion, and may possibly be required for secretion of these proteins
-
-
?
additional information
?
-
the enzyme is part of the endoplasmic reticulum and Golgi glycosylation network, glycosyltransferase metabolism, overview
-
-
?
additional information
?
-
-
HP0826 is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori
-
-
?
additional information
?
-
-
enzyme activity might be involved in apoptotic pathway in hepatocarcinoma cells
-
-
?
additional information
?
-
-
decreasing the expression of GalT V in glioma cells promotes apoptosis, inhibits the invasion and migration and the ability of tumor formation in vivo, and reduces the activation of AKT, the enzyme functions as a glioma growth activator, overview, contribution of GalT V in the activation of AKT and MAPK kinase, contribution of serum or EGF in the transcription regulation of the GalT V gene, overview
-
-
?
additional information
?
-
-
downregulation of the enzyme inhibits CDK11(p58)-mediated apoptosis induced by cycloheximide, while ectopically expressed beta1,4-GT 1 increases CDK11p58-mediated apoptosis
-
-
?
additional information
?
-
-
the cell surface enzyme promotes apoptosis by inhibiting the epidermal growth factor receptor pathway via inhibition of the autophosphorylation of epidermal growth factor receptor especially at Try 1068, mechanism, overview
-
-
?
additional information
?
-
-
tumor beta-1,4-galactosyltransferase IV overexpression is closely associated with colorectal cancer metastasis and poor prognosis, relationships between tumor beta-1,4-GT-IV overexpression and clinicopathologic characteristics, overview
-
-
?
additional information
?
-
-
beta 1,4GalT V galactosylates the beta1,6-GlcNAc branch of N-glycans at the cell surface and of secreted glycoproteins, and functions as a positive regulator in glioma development, overview. As2O3-induced gioma cell apoptosis mechanism involves beta 1,4GalT V inhibition, overview
-
-
?
additional information
?
-
-
beta-1,4-galactosylransferase participates in the synthesis of Galbeta1-4-GlcNac disaccharide unit of glycoconjugates, overview
-
-
?
additional information
?
-
-
the specific inhibitors of the phosphatidylinositol 3-kinase LY294002 and wortmannin up-regulate beta1,4-galactosyltransferase I and thus sensitize SMMC-7721 human hepatocarcinoma cells to cycloheximide-induced apoptosis, overview
-
-
?
additional information
?
-
-
beta4Gal-T1 participates in the synthesis of Galbeta1-4-GlcNac disaccharide unit of glycoconjugates
-
-
?
additional information
?
-
-
LgtB is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori
-
-
?
additional information
?
-
-
the enzyme is involved in regulation of carbohydrate composition of milk during lactation, endocrine enzyme regulation, overview
-
-
?
additional information
?
-
-
beta-1,4-GalT I is a type II membrane-bound glycoprotein, which occurs the plasma membrane of different types of cells where it serves as a cell surface adhesion molecule mediating various cell-cell and cell-matrix interactions such as sperm-egg binding, cell spreading, migration, and neurite outgrowth
-
-
?
additional information
?
-
beta-1,4-GalT I is a type II membrane-bound glycoprotein, which occurs the plasma membrane of different types of cells where it serves as a cell surface adhesion molecule mediating various cell-cell and cell-matrix interactions such as sperm-egg binding, cell spreading, migration, and neurite outgrowth
-
-
?
additional information
?
-
-
beta-1,4-GalT-I plays a key role in the synthesis of selectin ligands such as sialy Lewis, sLex, and sulfated sLex, overview. beta-1,4-GalT-I may play an important role in the inflammation reaction
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.16 - 0.27
4-methylumbelliferyl beta-D-xylopyranoside
0.35 - 1.06
4-methylumbelliferyl-beta-D-xylopyranoside
0.85 - 1.27
4-nitrophenyl beta-D-xylopyranoside
0.17
agalacto-poly-N-acetyllactosamine
-
-
-
2.8
agalactokeratan
-
-
-
0.2
alpha1-Acid glycoprotein
-
-
-
0.056 - 0.064
asialo-agalacto-transferrin
-
0.0608
asialogalactofetuin
-
-
-
6.28
galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-(N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-)mannosyl-beta-1,4-N-acetylglucosaminyl-beta-1,4-(fucosyl-alpha-1,6-)N-acetylglucosaminyl-asparagine
-
-
0.01
Glc-NAcbeta1-3Galbeta-O-naphthalenemethanol
-
0.6
GlcNAcbeta1,2-Manalpha1,3-Manbeta-OR
-
1.23
GlcNAcbeta1,2-Manalpha1,6 (GlcNAcbeta1,2-Manalpha1,3)Man
-
0.06
GlcNAcbeta1,2-Manalpha1,6-Manbeta-OR
-
0.71
GlcNAcbeta1,4-Manalpha1,3-Manbeta-OR
-
0.25
glycopeptide from porcine immunoglobulin G
-
-
-
0.02
immunoglobulin G heavy chain
-
-
-
0.83
lacto-N-triaosylceramide
-
-
0.19
lacto-N-triose II
-
-
0.01
N-acetyl-beta-D-glucosamine
-
1.59
N-acetyl-beta-D-glucosaminyl-1,4-N-acetyl-beta-D-glucosamine
-
850
N-acetylgalactosamine
-
-
0.0007 - 40
N-acetylglucosamine
0.43
N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-(galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-)mannosyl-beta-1,4-N-acetylglucosaminyl-beta-1,4-(fucosyl-alpha-1,6-)N-acetylglucosaminyl-asparagine
-
-
0.13
N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-(N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-)mannosyl-beta-1,4-N-acetylglucosaminyl-beta-1,4-(fucosyl-alpha-1,6-)N-acetylglucosaminyl-asparagine
-
-
3.4
N-acetylglucosaminyl-beta-1,3-(galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,6-)galactose
-
-
1.5
N-acetylglucosaminyl-beta-1,6-galactose
-
N-acetylglucosaminyl-beta-1,3-(N-acetylglucosaminyl-beta-1,6-)galactose
0.66
p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
-
-
0.79
pNP-beta-GlcNAc
-
pH 7.2
0.22 - 0.4
UDP-alpha-D-galactose
0.0105 - 0.31
UDP-galactose
4
UDP-glucuronic acid
-
Km above 4 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
10
UDP-N-acetyl-alpha-D-glucosamine
-
Km above 10 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.16
UDP-xylose
-
purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.0108 - 0.25
UDPgalactose
additional information
additional information
-
0.16
4-methylumbelliferyl beta-D-xylopyranoside
-
purified beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.27
4-methylumbelliferyl beta-D-xylopyranoside
-
purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.35
4-methylumbelliferyl-beta-D-xylopyranoside
mutant enzyme H195R, at pH 7.0 and 37°C
0.35
4-methylumbelliferyl-beta-D-xylopyranoside
recombinant wild type enzyme, at pH 7.0 and 37°C
0.44
4-methylumbelliferyl-beta-D-xylopyranoside
mutant enzyme R226A, at pH 7.0 and 37°C
0.46
4-methylumbelliferyl-beta-D-xylopyranoside
mutant enzyme R226K, at pH 7.0 and 37°C
0.54
4-methylumbelliferyl-beta-D-xylopyranoside
mutant enzyme R270K, at pH 7.0 and 37°C
0.55
4-methylumbelliferyl-beta-D-xylopyranoside
mutant enzyme H195Q, at pH 7.0 and 37°C
0.59
4-methylumbelliferyl-beta-D-xylopyranoside
mutant enzyme Y199F, at pH 7.0 and 37°C
0.6
4-methylumbelliferyl-beta-D-xylopyranoside
mutant enzyme R270A, at pH 7.0 and 37°C
0.64
4-methylumbelliferyl-beta-D-xylopyranoside
mutant enzyme H195A, at pH 7.0 and 37°C
1.06
4-methylumbelliferyl-beta-D-xylopyranoside
mutant enzyme Y196F, at pH 7.0 and 37°C
0.85
4-nitrophenyl beta-D-xylopyranoside
-
purified beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
1.27
4-nitrophenyl beta-D-xylopyranoside
-
purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.056
asialo-agalacto-transferrin
-
enzyme in pCMGT1-transfected cells
-
0.064
asialo-agalacto-transferrin
-
recombinant enzyme
-
1
D-glucose
-
recombinant enzyme
1.1
D-glucose
-
recombinant N-deglycosylated enzyme
0.0007
N-acetylglucosamine
-
cosubstrate: UDP-galactose, in presence of alpha-lactalbumin, mutant enzyme R228K
0.0013
N-acetylglucosamine
-
cosubstrate: UDP-glucose, in presence of alpha-lactalbumin, mutant enzyme R228K
0.0025
N-acetylglucosamine
-
cosubstrate: UDP-glucose, in presence of alpha-lactalbumin, wild-type enzyme
0.0039
N-acetylglucosamine
-
-
0.009
N-acetylglucosamine
-
cosubstrate: UDP-glucose, without alpha-lactalbumin, mutant enzyme R228K
0.011
N-acetylglucosamine
-
cosubstrate: UDP-galactose, without alpha-lactalbumin, mutant enzyme R228K
0.011
N-acetylglucosamine
-
cosubstrate: UDP-galactose, without alpha-lactalbumin, wild-type enzyme
0.074
N-acetylglucosamine
-
cosubstrate: UDP-glucose, without alpha-lactalbumin, wild-type enzyme
0.33
N-acetylglucosamine
-
enzyme from liver
0.5
N-acetylglucosamine
-
-
1
N-acetylglucosamine
-
-
1.49
N-acetylglucosamine
-
-
1.6
N-acetylglucosamine
-
-
1.9
N-acetylglucosamine
-
enzyme in pCMGT1-transfected cells
1.9
N-acetylglucosamine
-
N-acetylglucosaminyl-beta-1,3-galactose
2.3
N-acetylglucosamine
-
-
2.5
N-acetylglucosamine
-
recombinant enzyme
2.8
N-acetylglucosamine
-
-
3.3
N-acetylglucosamine
-
-
3.6
N-acetylglucosamine
-
recombinant enzyme
4
N-acetylglucosamine
-
-
4.6
N-acetylglucosamine
-
-
5.8
N-acetylglucosamine
-
-
8.2
N-acetylglucosamine
-
-
8.3
N-acetylglucosamine
-
recombinant N-deglycosylated enzyme
10
N-acetylglucosamine
-
recombinant enzyme
40
N-acetylglucosamine
-
-
0.054
ovalbumin
-
-
-
0.22
UDP-alpha-D-galactose
recombinant wild type enzyme, at pH 7.0 and 37°C
0.27
UDP-alpha-D-galactose
mutant enzyme R270A, at pH 7.0 and 37°C
0.29
UDP-alpha-D-galactose
mutant enzyme R226K, at pH 7.0 and 37°C
0.32
UDP-alpha-D-galactose
mutant enzyme H195R, at pH 7.0 and 37°C
0.32
UDP-alpha-D-galactose
mutant enzyme Y199F, at pH 7.0 and 37°C
0.33
UDP-alpha-D-galactose
mutant enzyme H195Q, at pH 7.0 and 37°C
0.34
UDP-alpha-D-galactose
mutant enzyme R226A, at pH 7.0 and 37°C
0.34
UDP-alpha-D-galactose
mutant enzyme Y196F, at pH 7.0 and 37°C
0.37
UDP-alpha-D-galactose
mutant enzyme R270K, at pH 7.0 and 37°C
0.4
UDP-alpha-D-galactose
mutant enzyme H195A, at pH 7.0 and 37°C
0.00491
UDP-Gal
-
-
0.0105
UDP-galactose
-
cosubstrate: N-acetylglucosamine, in presence of alpha-lactalbumin, mutant enzyme R228K
0.087
UDP-galactose
-
cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, mutant enzyme R228K
0.093
UDP-galactose
-
cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, wild-type enzyme
0.23
UDP-galactose
-
purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.31
UDP-galactose
-
purified beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.031
UDP-glucose
-
cosubstrate: N-acetylglucosamine, in presence of alpha-lactalbumin, wild-type enzyme
0.044
UDP-glucose
-
cosubstrate: N-acetylglucosamine, in presence of alpha-lactalbumin, mutant enzyme R228K
0.091
UDP-glucose
-
cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, mutant enzyme R228K
0.148
UDP-glucose
-
cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, wild-type enzyme
0.28
UDP-glucose
-
purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.0108
UDPgalactose
-
-
0.0118
UDPgalactose
-
enzyme from liver microsomal membrane
0.01205
UDPgalactose
-
enzyme from serum
0.024
UDPgalactose
-
enzyme in pCMGT1-transfected cells
0.025
UDPgalactose
-
recombinant enzyme
0.028
UDPgalactose
-
recombinant enzyme
0.052
UDPgalactose
-
enzyme from serum
0.065
UDPgalactose
-
enzyme from liver
0.091
UDPgalactose
-
recombinant enzyme
0.143
UDPgalactose
-
recombinant N-deglycosylated enzyme
0.2
UDPgalactose
-
reaction with glucose, wild-type enzyme and N-deglycosylated recombinant enzyme
0.21
UDPgalactose
-
reaction with glucose, recombinant enzyme
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
effects of cationic polypeptides
-
additional information
additional information
-
Km-value for ovalbumin for the N-deglycosylated recombinant enzyme is 6 mg/ml, and 15 mg/ml for th recombinant enzyme
-
additional information
additional information
kinetics, implications of the oligosaccharide structures on the kinetic parameters, overview
-
additional information
additional information
-
kinetics, mutant enzyme, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
C134S
-
complete loss of activity
C342S
-
33fold increase in the apparent Km-value for UDPgalactose
D254E
-
0.01% of the activity of the wild-type enzyme
D254N
-
0.01% of the activity of the wild-type enzyme
D320A
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
D320E
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
D320N
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
E317A
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
E317D
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
E317Q
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
H347D
-
in presence of Mn2+ retains 0.02% of wild-type enzyme activity, in presence of Co2+ retains 0.085% of wild-type enzyme activity
H347E
-
in presence of Mn2+ retains 0.1% of wild-type enzyme activity, in presence of Co2+ retains 0.4% of wild-type enzyme activity
H347N
-
in presence of Mn2+ retains 0.07% of wild-type enzyme activity, in presence of Co2+ retains 0.36% of wild-type enzyme activity
H347Q
-
in presence of Mn2+ retains 0.28% of wild-type enzyme activity, in presence of Co2+ retains 1.21% of wild-type enzyme activity
M344A
-
in presence of Mn2+ retains 54.5% of wild-type enzyme activity, in presence of Co2+ retains 6.15% of wild-type enzyme activity
M344H
-
site-directed mutagenesis, substrate binding structure in comparison to the wild-type enzyme, overview
M344Q
-
in presence of Mn2+ retains 15.37% of wild-type enzyme activity, in presence of Co2+ retains 31.08% of wild-type enzyme activity
R228K
-
mutant enzyme shows 16% of the wild-type galactosyltransferase activity, mutation results in a 15fold higher glucosyltransferase activity, which is further enhanced by alpha-lactalbumin to nearly 25% of the galactosyltransferase activity of the wild type. The main effect of the mutation is on the kcat of glucosyltransferase, which increases 3-4fold, both in the absence and in the presence of alpha-lactalbumin simultaneously, the kcat for the galactosyltransferase reaction is reduced 30fold
Y289I
-
mutation enhances GalNAc-transferase activity. Km for GlcNAc is increased compared to the wild type
Y289L
-
mutation enhances GalNAc-transferase activity. Km for GlcNAc is incereased compared to the wild type
Y289N
-
mutation enhances GalNAc-transferase activity. Km for GlcNAc is increased compared to the wild type
I289Y
-
mutation of the Drosophila beta4GalNAc-T1 converts the enzyme to a beta-1,4-galactosyltransferase-1, beta4Gal-T1, by reducing its beta4GalNAc-T1 activity by nearly 1000fold while enhancing its beta4Gal-T1 activity by 80fold
H195A
the mutant shows reduced activity compared to the wild type enzyme
H195Q
the mutant shows reduced activity compared to the wild type enzyme
H195R
the mutant shows reduced activity compared to the wild type enzyme
M340H
site-directed mutagenesis, substrate binding structure in comparison to the wild-type enzyme, overview
M344H
-
in the presence of Mg2+ the mutant exhibits 25% of the catalytic activity compared to the wild type enzyme in the presence of Mn2+. Although the mutant has higher Km in the presence of Mg2+ for the substrates compared to the wild type enzyme in the presence of Mn2+, the catalytic efficiency with respect to donor and acceptor has decreased by an order of about 13 and 6, respectively. The turnover number of the mutant is only reduced to 60%
R226A
the mutant shows reduced activity compared to the wild type enzyme
R226K
the mutant shows reduced activity compared to the wild type enzyme
R228K
-
the mutation enhances the glucosyltransferase activity of beta4GalNAc-T1, which is low for the wild-type enzyme, by steric alterations, overview
R228LK
-
the mutation enhances glucosyltransferase activity
R270A
the mutant shows reduced activity compared to the wild type enzyme
R270K
the mutant shows reduced activity compared to the wild type enzyme
W312C/P401C
-
site-directed mutagenesis, the mutations facilitate invitro folding of the recombinantly expressed enzyme
W312C/P401C/M340H
-
site-directed mutagenesis, substrate binding structure in comparison to the wild-type enzyme, overview
Y196F
the mutant shows reduced activity compared to the wild type enzyme
Y199F
the mutant shows reduced activity compared to the wild type enzyme
Y268G
-
site-directed mutagenesis, the mutant enzyme is less active compared to the wild-type enzyme
Y268G/Y294G
-
site-directed mutagenesis, the mutant enzyme is less active compared to the wild-type enzyme
Y285I
-
site-directed mutagenesis, the mutation converts the betaGALT1 enzyme into an equally efficient beta4GalNAc-T1, EC 2.4.1.90
Y285L
-
site-directed mutagenesis, the mutation converts the betaGALT1enzyme into an equally efficient beta4GalNAc-T1, EC 2.4.1.90
Y289I
-
the mutation makes the enzyme equally as efficient as Gal- or GalNAc-transferase
Y289L/C342T
-
site-directed mutagenesis, the mutant is able to transfer GalNAc from the sugar donor UDP-GalNAc to the acceptor, GlcNAc, with efficiency as good as that of galactose from UDP-Gal, in contrast to the wild-type enzyme, mutant substrate specificity with different donor substrate and oligosaccharides as acceptor substrates, mass spectrometry product analysis, overview, the C342T mutation does not alter enzyme activity, but increases the enzyme stability at room temperature
Y289N
-
the mutation makes the enzyme equally as efficient as Gal- or GalNAc-transferase
Y294G
-
site-directed mutagenesis, the mutant enzyme is less active compared to the wild-type enzyme
Y289L
-
mutation of the beta4GalNAc-T1 converts the enzyme to a beta-1,4-galactosyltransferase-1, beta4Gal-T1. Substituting Tyr289 for Leu removes this restriction and the Tyr289Leu mutant can now transfer GalNAc or other Gal C2-analogues from their respective UDPderivatives, not normally substrates of the enzyme, to the acceptor GlcNAc with the same efficiency with which the wild-type enzyme transfers Gal from UDP-Gal, phenotype, overview
Y289L
-
the mutation makes the enzyme equally as efficient as Gal- or GalNAc-transferase
additional information
-
N-terminal truncated forms of the enzyme between residues 1-129, do not show any significant difference in the apparent Km-values towards N-acetylglucosamine or linear oligosaccharide acceptors, e.g. for chitobiose and chitotriose, or for the nucleotide donor UDPgalactose. The binding behaviour of N-terminal and C-terminal fragments of the enzyme towards the N-acetylglucosamine-agarose and UDP-agarose columns differ, the former binds preferentially to the N-acetylglucosamine-columns, while the latter binds to UDP-agarose columns via Mn2+
additional information
-
mutations of Asp318 and Asp319 abolish enzyme activity
additional information
-
covalent immobilization of the enzyme in the absence of alpha-lactalbumin for use as biocatalyst, method evaluation, overview
additional information
-
knockdown of zebrafish beta-4GalT1 by two independent morpholino oligonucleotides results in embryos with a truncated anterior-posterior axis, as well as elongated somites and moderate defects in the patterning of the head mesenchyme, phenotype with the axial mesoderm of epiboly stage embryos being abnormally widened in beta-4GalT1 morphants, indicative of abnormal convergent extension, laminin isolated from beta-4GalT1 morphant embryos is poorly galactosylated, activity is restored by injection of wild-type enzyme mRNA, overview
additional information
-
autophosphorylation of epidermal growth factor receptor, EGFR, and phosphorylation of protein kinase B, PKB/Akt, and extracellular signalregulated protein kinase1/2,ERK1/2, which are downstream molecules of EGFR, are reduced in cell surface beta1,4GT1-overexpressing SMMC-7721 cells, phenotype, overview, knockdown of beta1,4GT1 by siRNA increases the autophosphorylation of EGFR at Tyr1068
additional information
-
RNAi-mediated knockdown of beta1,4-GT 1 inhibits CDK11(p58)-mediated apoptosis induced by cycloheximide, while ectopically expressed beta1,4-GT 1 increases CDK11p58-mediated apoptosis, enzyme knockout also inhibits the release of cytochrome c from mitochondria and caspase-3 processing, mechanism of beta1,4-GT 1 in CHX-induced apoptosis of CDK11p58-overexpressing cells, overview
additional information
-
beta 1,4GalT V antisense constructs interfere with beta 1,4GalT V leading to promotion of As2O3-induced glioma cell apoptosis, while overexpression of beta 1,4GalT V inhibits it, overview
additional information
-
introduction of human betaGalT into suspension cultured tobacco cells results in the production of recombinant proteins with galactose-extended glycans and decreased contents of beta(1,2)-xylose and alpha(1,3)-fucose
additional information
-
expression vectors bearing the luminal domains of beta4GalT-II and glucuronyltransferase show in GST-pulldown assays that the Golgi luminal domains are sufficient for the complex to form
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.