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NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
additional information
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NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
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NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
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NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
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NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
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NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
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NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
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additional information
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Thi4 is a suicidal enzyme undergoing only a single turnover
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additional information
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mechanism starts with cleavage of the N-glycosyl bond of NAD followed by ring opening, tautomerization and loss of water. Reaction continues with imine formation, tautomerization, sulfide addition and cyclization. Elimination of two water molecules followed by a tautomerization completes the formation of the thiazole moiety
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additional information
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mechanism starts with cleavage of the N-glycosyl bond of NAD followed by ring opening, tautomerization and loss of water. Reaction continues with imine formation, tautomerization, sulfide addition and cyclization. Elimination of two water molecules followed by a tautomerization completes the formation of the thiazole moiety
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metabolism
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the enzyme synthesizes the thiazole precursor of thiamin
metabolism
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the enzyme synthesizes the thiazole precursor of thiamin
metabolism
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the enzyme synthesizes the thiazole precursor of thiamin
metabolism
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the enzyme synthesizes the thiazole precursor of thiamin
physiological function
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an enzyme deletion mutant displays increased sensitivity to oxidative stress and enhanced thiamine diphosphate biosynthesis as compared with the wild-type strain
physiological function
enzyme is able to functionally replace Thi4 in yeast thiazole synthesis. CyPBP37 is a substrate of the chaperone activity of its own binding partner cyclophilin CyP41
physiological function
expression of Thi1 rescues a yeast Thi4 mutant when fused to the yeast Thi4 signal peptide
physiological function
protein Thi4 from Saccharomyces cerevisiae fails to catalyze the formation of the thiazole moiety from cysteine (or sulfide), glycine and a variety of C5 carbohydrates. Thi4 also fails to complement an Escherichia coli thiazole biosynthetic mutant ThiF. The ADP adduct of 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid is present at the active site of Thi4 and the carboxylic acid of the thiazole forms hydrogen bonding and electrostatic interactions with Arg301
physiological function
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enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period
physiological function
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enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period
physiological function
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enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period
physiological function
-
enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period
physiological function
-
protein Thi4 from Saccharomyces cerevisiae fails to catalyze the formation of the thiazole moiety from cysteine (or sulfide), glycine and a variety of C5 carbohydrates. Thi4 also fails to complement an Escherichia coli thiazole biosynthetic mutant ThiF. The ADP adduct of 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid is present at the active site of Thi4 and the carboxylic acid of the thiazole forms hydrogen bonding and electrostatic interactions with Arg301
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physiological function
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enzyme is able to functionally replace Thi4 in yeast thiazole synthesis. CyPBP37 is a substrate of the chaperone activity of its own binding partner cyclophilin CyP41
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Jurgenson, C.; Chatterjee, A.; Begley, T.; Ealick, S.
Structural insights into the function of the thiamin biosynthetic enzyme Thi4 from Saccharomyces cerevisiae
Biochemistry
45
11061-11070
2006
Saccharomyces cerevisiae (P32318), Saccharomyces cerevisiae ATCC 204508 (P32318)
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Structural basis for iron-mediated sulfur transfer in archael and yeast thiazole synthases
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55
1826-1838
2016
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THI1, a protein involved in the biosynthesis of thiamin in Arabidopsis thaliana Structural analysis of THI1(A140V) mutant
Biochim. Biophys. Acta
1844
1094-1103
2014
Arabidopsis thaliana (Q38814)
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Molecular cloning of thi-4, a gene necessary for the biosynthesis of thiamine in Neurospora crassa
Curr. Genet.
30
62-67
1996
Neurospora crassa (P79048), Neurospora crassa, Neurospora crassa DSM 1257 (P79048)
brenda
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Altered expression and activities of enzymes involved in thiamine diphosphate biosynthesis in Saccharomyces cerevisiae under oxidative and osmotic stress
FEMS Yeast Res.
12
534-546
2012
Saccharomyces cerevisiae
brenda
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Thiamin biosynthesis in eukaryotes Characterization of the enzyme-bound product of thiazole synthase from Saccharomyces cerevisiae and its implications in thiazole biosynthesis
J. Am. Chem. Soc.
128
7158-7159
2006
Saccharomyces cerevisiae (P32318), Saccharomyces cerevisiae ATCC 204508 (P32318)
brenda
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Biosynthesis of thiamin thiazole in eukaryotes Conversion of NAD to an advanced intermediate
J. Am. Chem. Soc.
129
2914-2922
2007
Saccharomyces cerevisiae (P32318)
brenda
Godoi, P.; Galhardo, R.; Luche, D.; Van Sluys, M.; Menck, C.; Oliva, G.
Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana
J. Biol. Chem.
281
30957-30966
2006
Arabidopsis thaliana (Q38814)
brenda
Faou, P.; Tropschug, M.
Neurospora crassa CyPBP37 A cytosolic stress protein that is able to replace yeast Thi4p function in the synthesis of vitamin B 1
J. Mol. Biol.
344
1147-1157
2004
Neurospora crassa (Q1K6I4), Neurospora crassa, Neurospora crassa DSM 1257 (Q1K6I4)
brenda
Abidin, A.; Yee, W.; Rahman, N.; Idris, Z.; Yusof, Z.
Osmotic, oxidative and salinity stresses upregulate the expressions of Thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis)
J. Oil Palm Res.
28
308-319
2016
Elaeis guineensis
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brenda
Chatterjee, A.; Abeydeera, N.; Bale, S.; Pai, P.; Dorrestein, P.; Russell, D.; Ealick, S.; Begley, T.
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase
Nature
478
542-546
2011
Saccharomyces cerevisiae (P32318)
brenda
Ribeiro, A.; Praekelt, U.; Akkermans, A.; Meacock, P.; Van Kammen, A.; Bisseling, T.; Pawlowski, K.
Identification of agthi1, whose product is involved in biosynthesis of the thiamine precursor thiazole, in actinorhizal nodules of Alnus glutinosa
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10
361-368
1996
Alnus glutinosa (Q38709), Arabidopsis thaliana (Q38814)
brenda
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Regulation of THI4(MOL1), a thiamine-biosynthetic gene of Saccharomyces cerevisiae
Yeast
10
481-490
1994
Saccharomyces cerevisiae, Saccharomyces cerevisiae PMY3
brenda
Joshi, J.; Beaudoin, G.; Patterson, J.; Garca-Garca, J.; Belisle, C.; Chang, L.; Li, L.; Duncan, O.; Millar, A.; Hanson, A.
Bioinformatic and experimental evidence for suicidal and catalytic plant THI4s
Biochem. J.
477
2055-2069
2020
Arabidopsis thaliana, Avena sativa, Hordeum vulgare, Triticum aestivum
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Sun, J.; Sigler, C.; Beaudoin, G.; Joshi, J.; Patterson, J.; Cho, K.; Ralat, M.; Gregory, J.; Clark, D.; Deng, Z.; Colquhoun, T.; Hanson, A.
Parts-prospecting for a high-efficiency thiamin thiazole biosynthesis pathway
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179
958-968
2019
Caladium bicolor (A0A3G2LN21)
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Redesigning thiamin synthesis Prospects and potential payoffs
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273
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Arabidopsis thaliana, Hordeum vulgare
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