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Information on EC 2.5.1.50 - zeatin 9-aminocarboxyethyltransferase for references in articles please use BRENDA:EC2.5.1.50
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EC Tree
IUBMB Comments The enzyme acts not only on zeatin but also on other N6-substituted adenines. The reaction destroys their cytokinin activity and forms the corresponding 3-(adenin-9-yl)-L-alanine.
The expected taxonomic range for this enzyme is: Lupinus luteus
Synonyms
4.2.99.13, beta-(9-cytokinin)-alanine synthase, beta-(9-cytokinin)alanine synthase, lupinic acid synthase, lupinic acid synthetase, O-acetyl-L-serine acetate-lyase (adding N6-substituted adenine), synthetase, lupinate,
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beta-(9-cytokinin)-alanine synthase
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beta-(9-cytokinin)alanine synthase
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lupinic acid synthase
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lupinic acid synthetase
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O-acetyl-L-serine acetate-lyase (adding N6-substituted adenine)
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synthetase, lupinate
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O-acetyl-L-serine + zeatin = lupinate + acetate
O-acetyl-L-serine + zeatin = lupinate + acetate
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O-acetyl-L-serine + zeatin = lupinate + acetate
ping pong bi bi mechanism
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aminopropanoyl-group transfer
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O3-acetyl-L-serine:zeatin 2-amino-2-carboxyethyltransferase
The enzyme acts not only on zeatin but also on other N6-substituted adenines. The reaction destroys their cytokinin activity and forms the corresponding 3-(adenin-9-yl)-L-alanine.
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O-acetyl-L-serine + 6-(2-hydroxyethylamino)purine
2-amino-3-(6-(2-hydroxyethyl)aminopurin-9yl)-propanoate + acetate
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20% of the activity with zeatin
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?
O-acetyl-L-serine + 6-(3,4-dimethoxybenzylamino)purine
2-amino-3-(6-(3,4-dimethoxybenzylamino)purin-9yl)-propanoate + acetate
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7.5% of the activity with zeatin
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O-acetyl-L-serine + 6-(6-hydroxyhexylamino)purine
2-amino-3-(6-aminohexylpurin-9yl)-propanoate + acetate
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64% of the activity with zeatin
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O-acetyl-L-serine + 6-(DELTA2-isopentenyl)aminopurine
2-amino-3-(6-(DELTA2-isopentenyl)aminopurin-9yl)-propanoate + acetate
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290% of the activity with zeatin
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O-acetyl-L-serine + 6-benzylaminopurine
2-amino-3-(6-benzylaminopurin-9yl)-propanoate + acetate
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as active as zeatin
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O-acetyl-L-serine + 6-furfurylaminopurine
2-amino-3-(6-furfurylaminopurin-9yl)-propanoate + acetate
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138% of the activity with zeatin
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O-acetyl-L-serine + 6-isopentylaminopurine
2-amino-3-(6-isopentylaminopurin-9yl)-propanoate + acetate
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99% of the activity with zeatin
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O-acetyl-L-serine + 6-methylaminopurine
2-amino-3-(6-methylaminopurin-9-yl)propanoate + acetate
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25.0% of the activity with zeatin
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O-acetyl-L-serine + 6-propylaminopurine
2-amino-3-(6-propylaminopurin-9yl)-propanoate + acetate
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57% of the activity with zeatin
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O-acetyl-L-serine + adenine
2-amino-3-(6-aminopurin-9-yl)-propanoate + acetate
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7.5% of the activity with zeatin
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O-acetyl-L-serine + cis-zeatin
lupinate + acetate
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91% of the activity with zeatin
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O-acetyl-L-serine + dihydrozeatin
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30% of the activity with zeatin
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O-acetyl-L-serine + O-beta-D-glucopyranosylzeatin
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34% of the activity with zeatin
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O-acetyl-L-serine + zeatin
lupinate + acetate
O-acetyl-L-serine + zeatin
lupinate + acetate
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O-acetyl-L-serine + zeatin
lupinate + acetate
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2,4-dichlorophenoxy acetic acid
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2,6-dichlorophenoxy acetic acid
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2-(indol-3-yl)propionic acid
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3-(indol-3-yl)propionic acid
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4,7-dichloroindol-3-yl-acetic acid
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4-chloroindol-3-yl acetic acid
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5,7-dichloroindol-3-yl-acetic acid
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indol-3-yl acetic acid
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indole-2-carboxylic acid
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indole-3-carboxylic acid
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N-(3-Chlorophenyl)-N'-phenylurea
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N-(3-nitrophenyl)-N'-phenylurea
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N-benzyl-N'-(3,4-dichlorophenyl)urea
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N-benzyl-N'-(3-chlorophenyl)urea
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N-benzyl-N'-phenylurea
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0.047
O-acetyl-serine
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0.002
2,4-dichlorophenoxy acetic acid
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0.034
2,6-dichlorophenoxy acetic acid
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7.2
2-(indol-3-yl)propionic acid
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2.8
3-(indol-3-yl)propionic acid
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0.002
4,7-dichloroindol-3-yl-acetic acid
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0.002
4-chloroindol-3-yl acetic acid
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0.0004
5,7-dichloroindol-3-yl-acetic acid
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0.07
indol-3-yl acetic acid
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0.14
indole-2-carboxylic acid
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1.1
indole-3-carboxylic acid
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0.22
N-(3-Chlorophenyl)-N'-phenylurea
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0.091
N-(3-nitrophenyl)-N'-phenylurea
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0.023
N-benzyl-N'-(3,4-dichlorophenyl)urea
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0.051
N-benzyl-N'-(3-chlorophenyl)urea
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0.026
N-benzyl-N'-phenylurea
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0.065
phenylacetic acid
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brenda
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brenda
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4�C, 2-3 months, stable
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Parker, C.W.; Entsch, B.; Letham, D.S.
Inhibitors of two enzymes which metabolize cytokinins
Phytochemistry
25
303-310
1986
Lupinus luteus
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brenda
Entsch, B.; Parker, C.W.; Letham, D.S.
An enzyme from lupin seeds forming alanine derivatives of cytokinins
Phytochemistry
22
375-381
1983
Lupinus luteus
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brenda
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2.5.1.50 zeatin 9-aminocarboxyethyltransferase
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