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IUBMB Comments The purine residue is transferred on to the apurinic site forming a normal glycosylic bond. dATP reacts at sites of the double-stranded depurinated DNA that lack adenine, and dGTP at sites that lack guanine.
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dATP + depurinated DNA = deoxyribose triphosphate + DNA
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nitrogenous group transfer
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dATP(dGTP):depurinated-DNA purine transferase
The purine residue is transferred on to the apurinic site forming a normal glycosylic bond. dATP reacts at sites of the double-stranded depurinated DNA that lack adenine, and dGTP at sites that lack guanine.
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dATP + depurinated DNA
deoxyribose triphosphate + purinated DNA
dATP + depurinated poly(dA-dT)
deoxyribose triphosphate + purinated poly(dA-dT)
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Substrates: dGTP cannot act as substrate
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dGTP + depurinated DNA
deoxyribose triphosphate + purinated DNA
dGTP + depurinated poly(dG-dC)
deoxyribose triphosphate + purinated poly(dG-dC)
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Substrates: dATP cannot act as substrate
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dTTP + depurinated DNA
deoxyribose triphosphate + purinated DNA
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Substrates: lower activity than that of dATP and dGTP, incorporation is supressed in the presence of dATP
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additional information
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dATP + depurinated DNA
deoxyribose triphosphate + purinated DNA
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Substrates: closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate
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dATP + depurinated DNA
deoxyribose triphosphate + purinated DNA
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Substrates: closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate
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dGTP + depurinated DNA
deoxyribose triphosphate + purinated DNA
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Substrates: closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate
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dGTP + depurinated DNA
deoxyribose triphosphate + purinated DNA
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Substrates: closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate
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additional information
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Substrates: specific insertion of adenine into adenine apurinic sites and guanine into guanine apurinic sites
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additional information
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Substrates: enzyme does not act on depurinated single-stranded DNA
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additional information
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Substrates: may represent a step in a repair mechanism, i.e. base-insertion repair, whereby apurinic sites, which may occur in double-stranded DNA either due to the removal of damaged purines with specific glycosylases or by spontaneous depurination, are directly filled with the correct missing purine base
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additional information
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Substrates: specific insertion of adenine into adenine apurinic sites and guanine into guanine apurinic sites
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additional information
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Substrates: possible alternative pathway for DNA repair
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additional information
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additional information
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Substrates: may represent a step in a repair mechanism, i.e. base-insertion repair, whereby apurinic sites, which may occur in double-stranded DNA either due to the removal of damaged purines with specific glycosylases or by spontaneous depurination, are directly filled with the correct missing purine base
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additional information
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Substrates: possible alternative pathway for DNA repair
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K+
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specific requirement, cannot be replaced by NH4+, Na+, Mg2+ or Mn2+
Li+
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supports activity to a small degree
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EDTA
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complete inhibition at 1.3 mM
additional information
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inhibited by high salt concentration e.g. 1 M NaCl
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Caffeine
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Caffeine
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20-80% inhibition when present at 0.1-1 mM
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assay at
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cultured
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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120000
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glycerol density gradient centrifugation
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complete inactivation after 15 min at 60°C
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sensitive to freezing and heating, extremely sensitive to dialysis
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unstable, activity retained for less than 1 week after purification, addition of phenylmethylsulfonyl fluoride or N-alpha-p-tosyl-L-lysine chloromethylketone hydrochloride can stabilize enzymatic fractions
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partial, using DEAE-cellulose and phosphocellulose chromatography followed by glycerol gradient centrifugation
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Deutsch, W.A.; Linn, S.
DNA binding activity from cultured human fibrolasts that is specific for partially depurinated DNA and that inserts purines into apurinic sites
Proc. Natl. Acad. Sci. USA
76
141-144
1979
Homo sapiens
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Livneh, Z.; Elad, D.; Sperling, J.
Enzymatic insertion of purine bases into depurinated DNA in vitro
Proc. Natl. Acad. Sci. USA
76
1089-1093
1979
Escherichia coli
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