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UDP-alpha-D-galactose + undecaprenyl phosphate
UMP + alpha-D-galactosyl-diphosphoundecaprenol
UDP-alpha-D-glucose + undecaprenyl phosphate
UMP + alpha-D-glucosyl-diphosphoundecaprenol
UDP-glucose + ditrans,octacis-undecaprenyl phosphate
UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
additional information
?
-
UDP-alpha-D-galactose + undecaprenyl phosphate
UMP + alpha-D-galactosyl-diphosphoundecaprenol
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-
-
-
?
UDP-alpha-D-galactose + undecaprenyl phosphate
UMP + alpha-D-galactosyl-diphosphoundecaprenol
-
-
-
-
?
UDP-alpha-D-galactose + undecaprenyl phosphate
UMP + alpha-D-galactosyl-diphosphoundecaprenol
-
-
-
-
?
UDP-alpha-D-glucose + undecaprenyl phosphate
UMP + alpha-D-glucosyl-diphosphoundecaprenol
-
UDP-alpha-D-glucose is the preferred substrate
-
-
?
UDP-alpha-D-glucose + undecaprenyl phosphate
UMP + alpha-D-glucosyl-diphosphoundecaprenol
-
UDP-alpha-D-glucose is the preferred substrate
-
-
?
UDP-alpha-D-glucose + undecaprenyl phosphate
UMP + alpha-D-glucosyl-diphosphoundecaprenol
-
UDP-alpha-D-glucose is the preferred substrate
-
-
?
UDP-glucose + ditrans,octacis-undecaprenyl phosphate
UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
-
the enzyme is involved in biosynthesis of xanthan
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-
?
UDP-glucose + ditrans,octacis-undecaprenyl phosphate
UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
-
EDTA-treated Xanthomonas campestris cells are used as both enzyme preparation and lipid-P acceptor, and UDP-Glc, GDP-Man, and UDP-glucuronic acid are used as sugar donors. A linear pentasaccharide unit is assembled on a polyprenol-P lipid carrier by the sequential addition of glucose-1-P, glucose, mannose, glucuronic acid, and mannose
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-
?
UDP-glucose + ditrans,octacis-undecaprenyl phosphate
UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
-
the C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity
-
-
?
UDP-glucose + ditrans,octacis-undecaprenyl phosphate
UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
-
GumD is involved in biosynthesis of the pentasaccharide repeating unit of xanthan. It is suggested that the wild-type Xanthomonas oryzae-produced xanthan is assembled by the sequential addition of UDP-glucose, UDP-glucose, GDP-mannose, UDPglucuronic acid, and GDP-mannose onto a polyprenol phosphate carrier, by the glycosyltransferase homologues encoded by the gumD, gumM, gumH, gumK, and gumI genes, respectively
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-
?
additional information
?
-
-
the enzyme catalyzes the initial step of the xanthan biosynthesis that transfers a glucose-phosphate residue from UDP-glucose to an isoprenoid lipid carrier
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-
?
additional information
?
-
-
the enzyme catalyzes the initial step of the xanthan biosynthesis that transfers a glucose-phosphate residue from UDP-glucose to an isoprenoid lipid carrier
-
-
?
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UDP-glucose + ditrans,octacis-undecaprenyl phosphate
UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
UDP-glucose + ditrans,octacis-undecaprenyl phosphate
UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
-
the enzyme is involved in biosynthesis of xanthan
-
-
?
UDP-glucose + ditrans,octacis-undecaprenyl phosphate
UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
-
GumD is involved in biosynthesis of the pentasaccharide repeating unit of xanthan. It is suggested that the wild-type Xanthomonas oryzae-produced xanthan is assembled by the sequential addition of UDP-glucose, UDP-glucose, GDP-mannose, UDPglucuronic acid, and GDP-mannose onto a polyprenol phosphate carrier, by the glycosyltransferase homologues encoded by the gumD, gumM, gumH, gumK, and gumI genes, respectively
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-
?
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metabolism
the GumD protein catalyses the first step of xanthan production and is responsible for the addition of the first glucose molecule to the isoprenoid acceptor molecule
malfunction
a gumD mutant, isolated from Xc17 by gene replacement possesses altered pigment xanthomonadin profiles and exhibits reduced virulence in causing black rot in broccoli
malfunction
-
from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumD completely abolishes in vitro polymer formation. The gumD- mutant is the unique mutant strain, generated in a wild-type background, that shows no released labeled oligosaccharides when permeabilized cells are labeled with UDP-[14C]glucose. Cells labeled with UDP-[14C]glucuronic acid or GDP-[14C]mannose show similar results. The C-terminal portion of GumD is cloned into broad-host-range vector pRK404, producing plasmid pCD2. This plasmid complements the xanthan defect in the XcD strain, rendering mucoid colonies
malfunction
-
significantly attenuated production of xanthan in knock-out mutant strain SJ1017
malfunction
-
xanthan-deficient mutants of Xanthomonas axonopodis pv. citri, the bacterium responsible for citrus canker, are generated by deletion and marker exchange of the region encoding the carboxy-terminal end of the first glycosyltransferase, GumD. Mutants of gumD do not produce xanthan and remain pathogenic in citrus plants to the same extent as wild-type bacteria. The kinetics of appearance of initial symptoms, areas of plant material affected, and growth of bacteria inside plant tissue throughout the disease process are similar for both wild-type and mutant inoculations. Exopolysaccharide deffciency does not impair the ability of the bacteria to induce hypersensitive response on non-host plants. Apart from variations in phenotypic aspects, no differences in growth or survival under diVerent stress conditions are observed between the xanthan-deficient mutant and wild-type bacteria. gumD mutants display impaired survival under oxidative stress during stationary phase as well as impaired epiphytic survival on citrus leaves
malfunction
extracellular polysaccharide-defective mutants of Xanthomonas axonopodis pv. manihotis are created by targeted disruption of the gumD gene
malfunction
-
the gumD deletion mutant exhibits reduced biofilm and extracellular polysaccharides production and attenuated virulence on rice
malfunction
-
the gumD deletion mutant is negative in xanthan production, grows well, but its colonies are smaller and non-mucoid
malfunction
-
the gumD deletion mutant is negative in xanthan production, grows well, but its colonies are smaller and non-mucoid
-
physiological function
-
GumH is involved in biosynthesis of the pentasaccharide repeating unit of xanthan
physiological function
-
the enzyme is involved in biosynthesis of the exopolysaccharide xanthan
physiological function
-
the enzyme is involved in biosynthesis of xanthan
physiological function
Xanthomonas axonopodis pv. manihotis gumD gene is essential for extracellular polysaccharide production and pathogenicity and enhances epiphytic survival on cassava (Manihot esculenta)
physiological function
-
gumD-dependent extracellular polysaccharides contribute to biofilm formation
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Dunger, G.; Relling, V.M.; Tondo, M.L.; Barreras, M.; Ielpi, L.; Orellano, E.G.; Ottado, J.
Xanthan is not essential for pathogenicity in citrus canker but contributes to Xanthomonas epiphytic survival
Arch. Microbiol.
188
127-135
2007
Xanthomonas axonopodis
brenda
Chou, F.L.; Chou, H.C.; Lin, Y.S.; Yang, B.Y.; Lin, N.T.; Weng, S.F.; Tseng, Y.H.
The Xanthomonas campestris gumD gene required for synthesis of xanthan gum is involved in normal pigmentation and virulence in causing black rot
Biochem. Biophys. Res. Commun.
233
265-269
1997
Xanthomonas campestris (Q56813)
brenda
Kim, S.Y.; Kim, J.G.; Lee, B.M.; Cho, J.Y.
Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae pv oryzae
Biotechnol. Lett.
31
265-270
2008
Xanthomonas oryzae
brenda
Ielpi, L.; Couso, R.O.; Dankert, M.A.
Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris
J. Bacteriol.
175
2490-2500
1993
Xanthomonas campestris
brenda
Pollock, T.J.; Thorne, L.; Yamazaki, M.; Mikolajczak, M.J.; Armentrout, R.W.
Mechanism of bacitracin resistance in gram-negative bacteria that synthesize exopolysaccharides
J. Bacteriol.
176
6229-6237
1994
Xanthomonas campestris
brenda
Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Phler, A.; Ielpi, L.
Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence
J. Bacteriol.
180
1607-1617
1998
Xanthomonas campestris
brenda
Kemp, B.P.; Horne, J.; Bryant, A.; Cooper, R.M.
Xanthomonas axonopodis pv. manihotis gumD gene is essential for EPS production and pathogenicity and enhances epiphytic survival on cassava (Manihot esculenta)
Physiol. Mol. Plant Pathol.
64
209-218
2004
Xanthomonas phaseoli pv. manihotis (Q6UTG5)
brenda
Patel, K.; Toh, E.; Fernandez, X.; Hanuszkiewicz, A.; Hardy, G.; Brun, Y.; Bernards, M.; Valvano, M.
Functional characterization of UDP-Glucose: Undecaprenyl-phosphate glucose-1-phosphate transferases of Escherichia coli and Caulobacter crescentus
J. Bacteriol.
194
2646-2657
2012
Caulobacter vibrioides, Escherichia coli, Caulobacter vibrioides CB15
brenda
Schatschneider, S.; Persicke, M.; Watt, S.A.; Hublik, G.; Puehler, A.; Niehaus, K.; Vorhoelter, F.J.
Establishment, in silico analysis, and experimental verification of a large-scale metabolic network of the xanthan producing Xanthomonas campestris pv. campestris strain B100
J. Biotechnol.
167
123-134
2013
Xanthomonas campestris pv. campestris, Xanthomonas campestris pv. campestris B100
brenda
Zhang, Y.; Wei, C.; Jiang, W.; Wang, L.; Li, C.; Wang, Y.; Dow, J.M.; Sun, W.
The HD-GYP domain protein RpfG of Xanthomonas oryzae pv. oryzicola regulates synthesis of extracellular polysaccharides that contribute to biofilm formation and virulence on rice
PLoS ONE
8
e59428
2013
Xanthomonas oryzae pv. oryzicola
brenda