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Information on EC 2.8.4.5 - tRNA (N6-L-threonylcarbamoyladenosine37-C2)-methylthiotransferase

for references in articles please use BRENDA:EC2.8.4.5

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IUBMB Comments

The enzyme, which is a member of the S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes superfamily, binds two [4Fe-4S] clusters as well as the transferred sulfur. The sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that in vitro the reaction is a single turnover. The identity of the electron donor is not known.

The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
hide
N6-L-threonylcarbamoyladenine37 in tRNA
+
sulfur-(sulfur carrier)
+
2
S-adenosyl-L-methionine
+
reduced electron acceptor
=
2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA
+
S-adenosyl-L-homocysteine
+
(sulfur carrier)
+
L-methionine
+
5'-deoxyadenosine
+
electron acceptor
+
sulfur-(sulfur carrier)
+
2
+
reduced electron acceptor
=
2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA
+
+
(sulfur carrier)
+
+
+
electron acceptor
N6-L-threonylcarbamoyladenine37 in tRNA
+
sulfur-(sulfur carrier)
+
S-adenosyl-L-methionine
+
reduced electron acceptor
=
2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA
+
(sulfur carrier)
+
L-methionine
+
5'-deoxyadenosine
+
electron acceptor
+
sulfur-(sulfur carrier)
+
+
reduced electron acceptor
=
2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA
+
(sulfur carrier)
+
+
+
electron acceptor
S-adenosyl-L-methionine
+
2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA
=
S-adenosyl-L-homocysteine
+
2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA
+
2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA
=
+
2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA

Synonyms
cdkal1, more

REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor
show the reaction diagram
N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced electron acceptor = 2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor
show the reaction diagram
S-adenosyl-L-methionine + 2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA = S-adenosyl-L-homocysteine + 2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA
show the reaction diagram
Highest Expressing Human Cell Lines
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