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ATGTGTCTGTGVAGACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
GTGTCTGTGVAGACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
GTTTTAAATTATGGAGTATGTCAVTGTGGAGACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
GTTTTAAATTATGGAGTATGTGAVTGTGGAGACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
GTTTTAAATTATGGAGTATGTGTCTGTGCAVACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
GTTTTAAATTATGGAGTATGTGTCTGTGGAVACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
GTTTTAAATTATGGAGTATGTGTCTGTGVAGACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
oligonucleotides + H2O
5'-phosphomononucleotides
PO4-TAT(-FAM)CCACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence
-
-
?
PO4-TCTCCACAYT(-FAM)CACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, Y = tetrahydrofuran abasic site
-
-
?
PO4-TUT(-FAM)ACACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence
-
-
?
PO4-TUT(-FAM)TCACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence
-
-
?
PO4-TXT(-FAM)TCACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, X = natural abasic site generated by UDG
-
-
?
PO4-TYT(-FAM)ACACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, Y = tetrahydrofuran abasic site
-
-
?
PO4-TYT(-FAM)CCACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, Y = tetrahydrofuran abasic site
-
-
?
PO4-TYT(-FAM)TCACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, Y = tetrahydrofuran abasic site
-
-
?
PO4-TYTCCACAGACACAT(-FAM)ACTCCATAATTTAA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, Y = tetrahydrofuran abasic site
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
TATGTGTCTGTGVAGACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
TGTGTCTGTGVAGACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
additional information
?
-
DNA + H2O
?
-
-
-
-
?
DNA + H2O
?
Lambdavirus lambda
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
133978, 133979, 133980, 133981, 133983, 133984, 133985, 133986, 133987, 133988, 133989, 133992 -
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
exonucleolytic cleavage in the 5'- to 3'-direction to yield 5'-phosphomononucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degradation also at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
possess "red" recombination system
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
high processivity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
no activity on denatured DNA or RNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades native DNA 100 times faster than long single-stranded DNA or oligonucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
shows strong preference for termini bearing 5'-phosphoryl groups
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
no attack at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
preference for a 5'-phosphoryl group over a 5'-hydroxyl group
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
lambda exonuclease is unable to initiate DNA digestion at nicks or gaps
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degradation also at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
-
133978, 133979, 133980, 133983, 133984, 133985, 133986, 133987, 133988, 133989, 133992 -
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
exonucleolytic cleavage in the 5'- to 3'-direction to yield 5'-phosphomononucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
possess "red" recombination system
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
high processivity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
no activity on denatured DNA or RNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
degrades native DNA 100 times faster than long single-stranded DNA or oligonucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
shows strong preference for termini bearing 5'-phosphoryl groups
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
no attack at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
preference for a 5'-phosphoryl group over a 5'-hydroxyl group
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda phage 80
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
no activity on denatured DNA or RNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
activity depends on lenght of double-stranded DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
high processivity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
lambda exonuclease selectively digests the phosphorylated undesired strand of double-stranded DNA and produces single-stranded DNA in high yield and purity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
sequence-dependent rate and pausing of individual lambda exonuclease enzymes during the digestion of a DNA sequence containing 120 CGG repeats. GC-rich repetitive sequences provide a significant barrier to lambda exonuclease activity by increasing the probability of pausing in addition to reducing the average digestion rate
-
-
?
oligonucleotides + H2O
5'-phosphomononucleotides
-
strong preference for relatively short chain oligonucleotides
-
-
?
oligonucleotides + H2O
5'-phosphomononucleotides
-
low rate
-
-
?
oligonucleotides + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
strong preference for relatively short chain oligonucleotides
-
-
?
oligonucleotides + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
low rate
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
-
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
mechanism for stimulation of recombination
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
degrades the 5'-terminated strand at a single-stranded branch
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
rate of digestion and binding is inversely related to the chain lenght
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
digestion not processively
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
at single-stranded DNA branches
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
-
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
mechanism for stimulation of recombination
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
degrades the 5'-terminated strand at a single-stranded branch
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
rate of digestion and binding is inversely related to the chain lenght
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
digestion not processively
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
binds to circular or linear single-stranded DNA
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
at single-stranded DNA branches
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
degrades ssDNA at higher enzyme concentrations
-
-
?
additional information
?
-
Lambdavirus lambda
-
for enzyme digestion of single DNA molecules, individual lambda-DNA molecules labeled with the fluorescent dye, YOYO-1, are stretched in a laminar flow stream and immobilized on a bare fused-silica prism surface based on hydrophobic and electrostatic interactions. Enzyme digestion is initiated by the influx of lambda-exonuclease enzyme via capillary force, method evaluation, overview. Progression of single-DNA digestion by lambda-exonuclease enzyme based on the decrease in the length of the DNA molecule at a dye:bp ratio of 1:5
-
-
?
additional information
?
-
Lambdavirus lambda
-
the enzyme unwinds the DNA prior to cleavage, such that two nucleotides of the 5'-ended strand insert into the active site of one subunit of the trimer, while the 3'-ended strand passes through the central channel to emerge out the back of the trimer. Unwinding of the DNA is facilitated by several apolar residues, including Leu78, that wedge into the base pairs at the single/double-strand junction to form favorable hydrophobic interactions. The terminal 5' phosphate of the DNA binds to a positively charged pocket buried at the end of the active site, while the scissile phosphate bridges two active site Mg2+ ions, mechanism of progressivity, structure-activity analysis, overview
-
-
?
additional information
?
-
Lambdavirus lambda
G-quadruplexes are resistant to lambda-exo digestion
-
-
?
additional information
?
-
Lambdavirus lambda
an ultrasensitive electrochemical biosensor for polynucleotide kinase assay based on gold nanoparticle-mediated lambda exonuclease cleavage-induced signal amplification is constructed. The presence of polynucleotide kinase (PNK) induces the phosphorylation of the strand 2-strand 1 hybrid and the subsequent cleavage of double-stranded DNA (dsDNA) by lambda exonuclease, resulting in the release of AuNP-strand 2 conjugates and [Ru(NH3)6]3+ from the gold electrode surface and consequently the decrease of electrochemical signal
-
-
?
additional information
?
-
Lambdavirus lambda
analysis of the ability of enzyme lambda-exo to efficiently digest G4 structures by digesting a plasmid derived from the human MYC locus (from MCF-7 breast cancer cells) that contains a well-characterized G4 motif, overview. Genome-wide biases of lambda-exo are profiled by sequencing the DNA remaining after lambda-exo digestion of sonicated genomic DNA (gDNA) from nonreplicating G0 MCF7 cells (LexoG0). The enzyme does not efficiently digest G-quadruplex (G4) structures in a plasmid. And enzyme lambda-exo digestion of nonreplicating genomic DNA (LexoG0) enriches GC-rich DNA and G4 motifs genome-wide
-
-
?
additional information
?
-
Lambdavirus lambda
sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease, substrate is target ssDNA with diverse DNA sequences, overview. Substrate specificity for base positions, discrimination factors of C:C type mismatched probe/target duplex, discrimination factors (DF) for duplexes containing different types of mismatched base pairs at position +I by using the abasic site modified probe in the absence and presence of different blocker strands, tested blocker sequences for the targets and type of mismatched base pair, overview
-
-
?
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Pierce, J.R.; Nazimiec, M.; Tang, M.s.
Comparison of sequence preference of tomaymycin- and anthramycin-DNA bonding by exonuclease III and lambda exonuclease digestion and UvrABC nuclease incision analysis
Biochemistry
32
7069-7078
1993
Lambdavirus lambda, Escherichia coli
brenda
Lehman, I.R.
Bacterial deoxyribonucleases
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
251-270
1971
Lambdavirus lambda, Escherichia coli
-
brenda
Mitsis, P.G.; Kwagh, J.G.
Characterization of the interaction of lambda exonuclease with the ends of DNA
Nucleic Acids Res.
27
3057-3063
1999
Lambdavirus lambda, Escherichia coli
brenda
Little, J.W.
Lambda exonuclease
Gene Amplif. Anal.
2
135-145
1981
Lambdavirus lambda, Escherichia coli
brenda
Kerr, C.; Sadowski, P.D.
Gene 6 exonuclease of bacteriophage T7. I. Purification and properties of the enzyme
J. Biol. Chem.
247
305-310
1972
Escherichia phage T7, Escherichia coli
brenda
Lindahl, T.
An exonuclease specific for double-stranded DNA: deoxyribonuclease from rabbit tissues
Methods Enzymol.
21
148-153
1971
Oryctolagus cuniculus
-
brenda
Sriprakash, K.S.; Lundh, N.; Huh, M.M.O.; Radding, C.M.
The specificity of lambda exonuclease. Interactions with single-atranded DNA
J. Biol. Chem.
250
5438-5445
1975
Lambdavirus lambda, Escherichia coli
brenda
Cassuto, E.; Radding, C.M.
Mechanism for the action of lambda exonuclease in genetic recombination
Nature New Biol.
229
13-16
1971
Lambdavirus lambda, Escherichia coli
brenda
Radding, C.M.
The role of exonuclease and beta protein of bacteriophage lambda in genetic recombination. I. Effects of red mutants on protein structure
J. Mol. Biol.
52
491-499
1970
Lambdavirus lambda, Escherichia coli
brenda
Little, J.W.; Lehman, I.R.; Kaiser, A.D.
An exonuclease induced by bacteriophage lambda. I. Preparation of the crystalline enzyme
J. Biol. Chem.
242
672-678
1967
Escherichia coli, Lambdavirus lambda
brenda
Little, J.W.
An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction
J. Biol. Chem.
242
679-686
1967
Lambdavirus lambda, Escherichia coli
brenda
Radding, C.M.; Szpirer, J.; Thomas, R.
The structural gene for lambda exonuclease
Proc. Natl. Acad. Sci. USA
57
277-283
1967
Lambdavirus lambda, Escherichia coli, Lambdavirus lambda phage 80
brenda
Carter, D.M.; Radding, C.M.
The role of exonuclease and beta protein of phage lambda in genetic recombination. II. Substrate specificity and the mode of action of lambda exonuclease
J. Biol. Chem.
246
2502-2512
1971
Lambdavirus lambda, Escherichia coli
brenda
Dolberg, M.; Baur, C.P.; Knippers, R.
Purification and characterization of a novel 5' exodeoxyribonuclease from the yeast Saccharomyces cerevisiae
Eur. J. Biochem.
198
783-787
1991
Saccharomyces cerevisiae
brenda
Rudd, E.A.
Reversible inhibition of lambda exonuclease with high pressure
Biochem. Biophys. Res. Commun.
230
140-142
1997
Lambdavirus lambda, Escherichia coli
brenda
Kovall, R.A.; Matthews, B.W.
Structural, functional, and evolutionary relationships between lambda-exonuclease and the type II restriction endonucleases
Proc. Natl. Acad. Sci. USA
95
7893-7897
1998
Lambdavirus lambda, Escherichia coli
brenda
Kim, S.; Blainey, P.C.; Schroeder, C.M.; Xie, X.S.
Multiplexed single-molecule assay for enzymatic activity on flow-stretched DNA
Nat. Methods
4
397-399
2007
Lambdavirus lambda (P03697)
brenda
Balagurumoorthy, P.; Adelstein, S.J.; Kassis, A.I.
Method to eliminate linear DNA from mixture containing nicked circular, supercoiled, and linear plasmid DNA
Anal. Biochem.
381
172-174
2008
Escherichia coli
brenda
Song, C.; Zhao, M.
Real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by a singly labeled DNA-hairpin smart probe coupled with lambda exonuclease cleavage
Anal. Chem.
81
1383-1388
2009
Escherichia coli
brenda
Conroy, R.S.; Koretsky, A.P.; Moreland, J.
Lambda exonuclease digestion of CGG trinucleotide repeats
Eur. Biophys. J.
39
337-343
2010
synthetic construct
brenda
Avci-Adali, M.; Paul, A.; Wilhelm, N.; Ziemer, G.; Wendel, H.P.
Upgrading SELEX technology by using lambda exonuclease digestion for single-stranded DNA generation
Molecules
15
1-11
2010
synthetic construct
brenda
Kang, S.H.; Lee, S.; Yeung, E.S.
Digestion of individual DNA molecules by lambda-exonuclease at liquid-solid interface
Analyst
135
1759-1764
2010
Lambdavirus lambda
brenda
Zhang, J.; McCabe, K.A.; Bell, C.E.
Crystal structures of lambda exonuclease in complex with DNA suggest an electrostatic ratchet mechanism for processivity
Proc. Natl. Acad. Sci. USA
108
11872-11877
2011
Lambdavirus lambda
brenda
Yuan, Z.; Zhou, Y.; Gao, S.; Cheng, Y.; Li, Z.
Homogeneous and sensitive detection of microRNA with ligase chain reaction and lambda exonuclease-assisted cationic conjugated polymer biosensing
ACS Appl. Mater. Interfaces
6
6181-6185
2014
Lambdavirus lambda
brenda
Oliver-Calixte, N.J.; Uba, F.I.; Battle, K.N.; Weerakoon-Ratnayake, K.M.; Soper, S.A.
Immobilization of lambda exonuclease onto polymer micropillar arrays for the solid-phase digestion of dsDNAs
Anal. Chem.
86
4447-4454
2014
Lambdavirus lambda
brenda
Cui, L.; Li, Y.; Lu, M.; Tang, B.; Zhang, C.Y.
An ultrasensitive electrochemical biosensor for polynucleotide kinase assay based on gold nanoparticle-mediated lambda exonuclease cleavage-induced signal amplification
Biosens. Bioelectron.
99
1-7
2018
Lambdavirus lambda (P03697)
brenda
Wu, T.; Xiao, X.; Gu, F.; Zhao, M.
Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease
Chem. Commun. (Camb.)
51
17402-17405
2015
Lambdavirus lambda (P03697)
brenda
Foulk, M.; Urban, J.; Casella, C.; Gerbi, S.
Characterizing and controlling intrinsic biases of lambda exonuclease in nascent strand sequencing reveals phasing between nucleosomes and G-quadruplex motifs around a subset of human replication origins
Genome Res.
25
725-735
2015
Lambdavirus lambda (P03697)
-
brenda