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D-fructose 1,6-bisphosphate + H2O
?
slow substrate
-
-
?
D-fructose 1,6-bisphosphate + H2O
D-fructose 1-phosphate + phosphate
poor substrate
-
-
?
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
D-glycero-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-D-manno-heptose 1-phosphate + phosphate
D-sedoheptulose 1,7-bisphosphate + H2O
?
-
-
-
?
additional information
?
-
no activity with: fructose 6-phosphate, fructose 1-phosphate, alpha-D-glucose 1,6-bisphosphate, beta-D-glucose 1,6-bisphosphate, alpha-D-mannose 1,6-bisphosphate, and beta-D-mannose 1,6-bisphosphate, histidinol
-
-
?
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
kcat/Km for the beta-anomer is 150fold higher than for the alpha-anomer
-
-
?
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
kcat/Km for the beta-anomer is 100fold higher than for the alpha-anomer
-
-
?
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
kcat/Km for the beta-anomer is 180fold higher than for the alpha-anomer
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
kcat/Km for the beta-anomer is 150fold higher than for the alpha-anomer
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
lipopolysaccharide is a major component of the outer membrane of Gram-negative bacteria and provides a permeability barrier to many commonly used antibiotics. ADP-heptose residues are an integral part of the LPS inner core, and mutants deficient in heptose biosynthesis demonstrate increased membrane permeability
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
it is suggested that GmhB functions through a phosphoaspartate intermediate
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
kcat/Km for the beta-anomer is 100fold higher than for the alpha-anomer
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
kcat/Km for the beta-anomer is 180fold higher than for the alpha-anomer
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-D-manno-heptose 1-phosphate + phosphate
synthesis of ADP-D-beta-D-heptose in Escherichia coli requires three proteins, GmhA (sedoheptulose 7-phosphate isomerase), HldE (bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase), and GmhB (D,D-heptose 1,7-bisphosphate phosphatase)
-
-
?
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D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
D-glycero-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-D-manno-heptose 1-phosphate + phosphate
synthesis of ADP-D-beta-D-heptose in Escherichia coli requires three proteins, GmhA (sedoheptulose 7-phosphate isomerase), HldE (bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase), and GmhB (D,D-heptose 1,7-bisphosphate phosphatase)
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
lipopolysaccharide is a major component of the outer membrane of Gram-negative bacteria and provides a permeability barrier to many commonly used antibiotics. ADP-heptose residues are an integral part of the LPS inner core, and mutants deficient in heptose biosynthesis demonstrate increased membrane permeability
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.68 - 1.5
D-fructose 1,6-bisphosphate
0.032 - 0.58
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
0.005 - 0.7
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
0.61
D-sedoheptulose 1,7-bisphosphate
pH 7.5, 25°C
0.68
D-fructose 1,6-bisphosphate
-
1.5
D-fructose 1,6-bisphosphate
pH 7.5, 25°C
0.032
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C107A
0.045
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C109A
0.067
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
0.067
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, wild-type
0.28
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
0.3
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C92A
0.58
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
0.005
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
0.005
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, wild-type
0.0069
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
0.013
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
0.039
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme K137A
0.068
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C109A
0.083
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C107A
0.18
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C92A
0.181
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme R110A
0.2
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 8.0, wild-type enzyme
0.7
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 8.0, mutant enzyme C107A
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.039 - 0.55
D-fructose 1,6-bisphosphate
0.0017 - 5.9
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
0.34 - 35.7
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
0.51
D-sedoheptulose 1,7-bisphosphate
pH 7.5, 25°C
0.039
D-fructose 1,6-bisphosphate
pH 7.5, 25°C
0.55
D-fructose 1,6-bisphosphate
-
0.0017
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C92A
0.042
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C107A
0.057
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C109A
4.4
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
4.6
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
4.6
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, wild-type
5.9
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
0.34
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C109A
0.63
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C107A
0.63
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C92A
2 - 8
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme K137A
3 - 6
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 8.0, wild-type enzyme
6.2
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme R110A
17
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 8.0, mutant enzyme C107A
18
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
22
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
35.7
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
35.7
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, wild-type
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.026
D-fructose 1,6-bisphosphate
pH 7.5, 25°C
0.006 - 69
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
3.5 - 7140
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
0.84
D-sedoheptulose 1,7-bisphosphate
pH 7.5, 25°C
0.006
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C92A
0.13
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C107A
1.3
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C109A
7.6
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
21
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
69
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
69
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, wild-type
3.5
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C92A
5
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C109A
7.6
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme C107A
34.3
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme R110A
718
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, mutant enzyme K137A
1385
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
3188
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
7140
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
7140
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C, wild-type
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Kim, M.S.; Shin, D.H.
A preliminary X-ray study of D,D-heptose-1,7-bisphosphate phosphatase from Burkholderia thailandensis E264
Acta Crystallogr. Sect. F
66
160-162
2010
Burkholderia thailandensis E264
brenda
Taylor, P.L.; Sugiman-Marangos, S.; Zhang, K.; Valvano, M.A.; Wright, G.D.; Junop, M.S.
Structural and kinetic characterization of the LPS biosynthetic enzyme D-alpha,beta-D-heptose-1,7-bisphosphate phosphatase (GmhB) from Escherichia coli
Biochemistry
49
1033-1041
2010
Escherichia coli (P63228)
brenda
Wang, L.; Huang, H.; Nguyen, H.H.; Allen, K.N.; Mariano, P.S.; Dunaway-Mariano, D.
Divergence of biochemical function in the HAD superfamily: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)
Biochemistry
49
1072-1081
2010
Escherichia coli (P63228), Bordetella bronchiseptica (Q7WG29), Mesorhizobium loti (Q98I56)
brenda
Nguyen, H.H.; Wang, L.; Huang, H.; Peisach, E.; Dunaway-Mariano, D.; Allen, K.N.
Structural determinants of substrate recognition in the HAD superfamily member D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)
Biochemistry
49
1082-1092
2010
Escherichia coli (P63228), Bordetella bronchiseptica (Q7WG29)
brenda
Plant, L.; Sundqvist, J.; Zughaier, S.; Lvkvist, L.; Stephens, D.S.; Jonsson, A.B.
Lipooligosaccharide structure contributes to multiple steps in the virulence of Neisseria meningitidis
Infect. Immun.
74
1360-1367
2006
Neisseria meningitidis
brenda
Kneidinger, B.; Marolda, C.; Graninger, M.; Zamyatina, A.; McArthur, F.; Kosma, P.; Valvano, M.A.; Messner, P.
Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli
J. Bacteriol.
184
363-369
2002
Escherichia coli (P63228)
brenda
Valvano, M.A.; Messner, P.; Kosma, P.
Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides
Microbiology
148
1979-1989
2002
Helicobacter pylori (O25531), Haemophilus influenzae (P46452), [Haemophilus] ducreyi (Q7VL21), Salmonella enterica subsp. enterica serovar Typhimurium (Q8ZRM8), Clostridium acetobutylicum (Q97EQ5), Pseudomonas aeruginosa (Q9I7C0), Neisseria meningitidis (Q9JWE9), Vibrio cholerae (Q9KTJ4), Helicobacter pylori J99 (Q9ZKY8)
brenda