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2'-AMP + H2O
adenosine + phosphate
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Substrates: -
Products: -
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3'-AMP + H2O
adenosine + phosphate
3'-CMP + H2O
cytosine + phosphate
3'-dAMP + H2O
3'-deoxyadenosine + phosphate
3'-dCMP + H2O
deoxycytosine + phosphate
3'-dGMP + H2O
deoxyguanosine + phosphate
3'-dTMP + H2O
deoxythymidine + phosphate
3'-GMP + H2O
guanosine + phosphate
3'-UMP + H2O
uridine + phosphate
adenosine 3',5'-bisphosphate + H2O
5'-AMP + phosphate
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Substrates: -
Products: -
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adenosine 3'-diphosphate 5'-phosphate
5'-AMP + diphosphate
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Substrates: -
Products: -
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ApA + H2O
adenosine + AMP
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Substrates: -
Products: -
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bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + phosphate
-
Substrates: almost no hydrolysis
Products: -
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deoxythymidine 3',5'-bisphosphate
5'-dTMP + phosphate
-
Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
DNA + H2O
?
-
Substrates: -
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
duplex deoxyadenosine-deoxythymine homopolymer + H2O
5'-dAMP + 5'-TMP + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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heteroduplex DNA + H2O
nicked DNA
platinum-DNA complexes + H2O
5'-phosphomononucleotides + platinum
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Substrates: native, denaturated and renaturated platinum-DNA complexes, use of several platinum compounds like diamminedichloroplatinum(II)
Products: -
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poly(U) + H2O
?
-
Substrates: preferred substrate
Products: -
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polyA + H2O
fragments of polyA
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Substrates: -
Products: -
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polyadenylic acid + H2O
5'-AMP + 5'-phosphooligonucleotides
polycytosylic acid + H2O
5'-CMP + 5'-phosphooligonucleotides
polydeoxyadenylic acid + H2O
5'-dAMP + 5'-phosphooligonucleotides
polydeoxycytosylic acid + H2O
5'-dCMP + 5'-phosphooligonucleotides
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Substrates: only little degradation
Products: -
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polydeoxyguanylic acid + H2O
5'-dGMP + 5'-phosphooligonucleotides
polydeoxythymidylic acid + H2O
5'-dTMP + 5'-phosphooligonucleotides
polyguanylic acid + H2O
5'-GMP + 5'-phosphooligonucleotides
polyinosinic acid + H2O
5'-IMP + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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polyU + H2O
fragments of polyU
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Substrates: -
Products: -
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polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
RNA + H2O
5'-mononucleotides
-
Substrates: AMP-preferential endoexonuclease
Products: -
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
RNA + H2O
?
-
Substrates: -
Products: -
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RNA + H2O
fragments of RNA
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
ssDNA + H2O
fragments of ssDNA
tRNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
additional information
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3'-AMP + H2O
adenosine + phosphate
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Substrates: -
Products: -
?
3'-AMP + H2O
adenosine + phosphate
-
Substrates: -
Products: -
?
3'-AMP + H2O
adenosine + phosphate
-
Substrates: -
Products: -
?
3'-AMP + H2O
adenosine + phosphate
-
Substrates: -
Products: -
?
3'-AMP + H2O
adenosine + phosphate
-
Substrates: -
Products: -
?
3'-CMP + H2O
cytosine + phosphate
-
Substrates: -
Products: -
?
3'-CMP + H2O
cytosine + phosphate
-
Substrates: -
Products: -
?
3'-CMP + H2O
cytosine + phosphate
-
Substrates: -
Products: -
?
3'-dAMP + H2O
3'-deoxyadenosine + phosphate
-
Substrates: -
Products: -
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3'-dAMP + H2O
3'-deoxyadenosine + phosphate
-
Substrates: only little activity
Products: -
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3'-dAMP + H2O
3'-deoxyadenosine + phosphate
-
Substrates: -
Products: -
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3'-dCMP + H2O
deoxycytosine + phosphate
-
Substrates: -
Products: -
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3'-dCMP + H2O
deoxycytosine + phosphate
-
Substrates: -
Products: -
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3'-dGMP + H2O
deoxyguanosine + phosphate
-
Substrates: -
Products: -
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3'-dGMP + H2O
deoxyguanosine + phosphate
-
Substrates: -
Products: -
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3'-dTMP + H2O
deoxythymidine + phosphate
-
Substrates: -
Products: -
?
3'-dTMP + H2O
deoxythymidine + phosphate
-
Substrates: -
Products: -
?
3'-GMP + H2O
guanosine + phosphate
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Substrates: -
Products: -
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3'-GMP + H2O
guanosine + phosphate
-
Substrates: -
Products: -
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3'-GMP + H2O
guanosine + phosphate
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Substrates: -
Products: -
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3'-UMP + H2O
uridine + phosphate
-
Substrates: -
Products: -
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3'-UMP + H2O
uridine + phosphate
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Substrates: -
Products: -
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3'-UMP + H2O
uridine + phosphate
-
Substrates: -
Products: -
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denatured DNA + H2O
?
-
Substrates: -
Products: -
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denatured DNA + H2O
?
-
Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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134959, 134960, 134961, 134962, 134963, 134965, 134966, 134967, 134968, 134970, 134971, 134972, 134973, 134974, 134975, 134976, 134977, 134978, 134979, 134980, 134981, 134982, 134983 Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: functional role in recombination and DNA repair in vivo
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: 75000 times more active on single-stranded than on native DNA
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: at acidic pH the enzyme is 25times more active on single-stranded DNA than on double-stranded DNA and 3times more active on single-stranded DNA than on single-stranded RNA. At neutral pH the RNase activity exceeds the DNase activity. The enzyme nicks supercoiled RF-I plasmid DNA and rapidly cuts the phosphodiester bond across from the nick in the resultant relaxed RF-II plasmid DNA.
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: native DNA hydrolysis probably by contaminating nuclease, its inactivation possible at 55°C
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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DNA + H2O
nicked DNA
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Substrates: -
Products: -
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DNA + H2O
nicked DNA
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Substrates: resolving of DNA-four way junction
Products: -
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DNA + H2O
nicked DNA
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Substrates: resolving of four-way junction
Products: -
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DNA + H2O
nicked DNA
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Substrates: T7 endonuclase I resolves four-way junctions by simultaeously introducing two nicks on the two continously stacked strands at sites 5' to the junction, also acts on a variety of DNA structures
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: low activity in comparision with ssDNA
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: sequence selective cleavage
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: activity on locally altered structures of native DNA like bleomycin-treated DNA, superhelical, depurinated or UV-irradiated DNA
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: only 2% of activity on ssDNA
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: only 2% of activity on ssDNA
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: single-strand-specific activity on Bluescript II KS+ supercoiled DNA is inherent property of snake venom exonuclease
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: probably exonucleolytic cleavage, acting on linear, but not circular dsDNA
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: higher rate of hydrolysis of alkylated and depurinated dsDNA than native DNA
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: 15-20fold higher activity on single-stranded than native DNA
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: cleavage of phage PM2 superhelical DNA to relaxed, nicked-circular form and unit length linear form molecules
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: activity on locally altered structures of native DNA like bleomycin-treated DNA, superhelical, depurinated or UV-irradiated DNA
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: cleavage of phage PM2 superhelical DNA to relaxed, nicked-circular form and unit length linear form molecules
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: after introduction of nicks and double-strand scissions liberation of 5'-phosphomononucleotides and 5'-phosphooligonucleotides from the newly created termini
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heteroduplex DNA + H2O
nicked DNA
Substrates: cleavage of single base pair mismatches in heteroduplex DNA
Products: -
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heteroduplex DNA + H2O
nicked DNA
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Substrates: cleavage of single base pair mismatches in heteroduplex DNA
Products: -
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heteroduplex DNA + H2O
nicked DNA
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Substrates: cleavage of single base pair mismatches in heteroduplex DNA
Products: -
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polyadenylic acid + H2O
5'-AMP + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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polyadenylic acid + H2O
5'-AMP + 5'-phosphooligonucleotides
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Substrates: only 5% of activity with denaturated DNA at pH 4.6, 0.05 M NaCl and 45°C, at pH 6.4 50% of activity in comparision with DNA
Products: -
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polyadenylic acid + H2O
5'-AMP + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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polyadenylic acid + H2O
5'-AMP + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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polycytosylic acid + H2O
5'-CMP + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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polycytosylic acid + H2O
5'-CMP + 5'-phosphooligonucleotides
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Substrates: only 5% of activity with denaturated DNA at pH 4.6, 0.05 M NaCl and 45°C, at pH 6.4 50% of activity in comparision with DNA
Products: -
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polycytosylic acid + H2O
5'-CMP + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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polycytosylic acid + H2O
5'-CMP + 5'-phosphooligonucleotides
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Substrates: only small degradation
Products: -
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polydeoxyadenylic acid + H2O
5'-dAMP + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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polydeoxyadenylic acid + H2O
5'-dAMP + 5'-phosphooligonucleotides
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Substrates: more slowly, but significant hydrolysis in comparision with denaturated DNA
Products: -
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polydeoxyguanylic acid + H2O
5'-dGMP + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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polydeoxyguanylic acid + H2O
5'-dGMP + 5'-phosphooligonucleotides
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Substrates: only little degradation, no degradation in the presence of Ca2+
Products: -
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polydeoxythymidylic acid + H2O
5'-dTMP + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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polydeoxythymidylic acid + H2O
5'-dTMP + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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polyguanylic acid + H2O
5'-GMP + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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polyguanylic acid + H2O
5'-GMP + 5'-phosphooligonucleotides
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Substrates: only 5% of activity with denaturated DNA at pH 4.6, 0.05 M NaCl and 45°C
Products: -
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polyguanylic acid + H2O
5'-GMP + 5'-phosphooligonucleotides
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Substrates: only small degradation
Products: -
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polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
-
Substrates: activity comparable with denaturated DNA at pH 4.6, 0.05 M NaCl and 45°C
Products: -
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polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
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Substrates: -
Products: -
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polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
-
Substrates: 80% of activity with polydeoxythymidylic acid as substrate
Products: -
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polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: 5fold more activity on DNA than RNA
Products: -
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: mainly 5'-phosphodi- and trinucleotides, at long incubation time more than 90% 5'-phosphomononucleotides
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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RNA + H2O
fragments of RNA
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Substrates: -
Products: -
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RNA + H2O
fragments of RNA
-
Substrates: -
Products: -
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RNA + H2O
fragments of RNA
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Substrates: -
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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134962, 134966, 134967, 134969, 134971, 134972, 134973, 134974, 134975, 134976, 134977, 134978, 134979, 134981, 134982, 134983 Substrates: -
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: mouse and guinea-pig satellite DNA
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: heat denaturated, 100°C, 5-15 min
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
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Substrates: no cleavage at single-base mismatches
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: specificity for phosphodiester bonds adjoining deoxycytidine
Products: 5'-phosphooligonucleotides with average chain lengh of about 50
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: no detectable 5'-phospho mononucleotides, most 5'-phospho tetranucleotides
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: mainly 5'-phosphooligonucleotides with 5-10 residues
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: heat denaturated, 100°C, 5-15 min
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: 15 times more active towards thermally denaturated than native DNA
Products: -
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ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Substrates: -
Products: -
?