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(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
beta-D-xylose + Xylbeta(1-6)Glcbeta(1-4)Glc
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
alpha-D-Xyl-(1->6)-beta-D-Glc + H2O
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
?
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
?
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc + H2O
?
-
-
-
?
apple unextractable solid + H2O
oligosaccharide + ?
Malus malus
-
-
-
-
?
azo-cellulose + H2O
oligosaccharide + ?
-
-
-
?
azuki bean cell wall + H2O
fragments of 50000 Da + ?
-
-
-
-
?
beta-glucan + H2O
oligosaccharide + ?
-
-
-
?
chemically modified cellulose + H2O
oligosaccharide + ?
oligoxyloglucan + H2O
isoprimeverose + ?
tamarind xyloglucan + H2O
oligosaccharide + ?
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
beta-D-xylose + (Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + isoprimeverose
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
Xylbeta(1-6)Glcbeta(1-4)Glc + H2O
Xylbeta(1-6)Glc + beta-D-glucopyranose
xyloglucan + H2O
isoprimeverose + ?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
additional information
?
-
(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
beta-D-xylose + Xylbeta(1-6)Glcbeta(1-4)Glc
-
-
-
-
?
(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
beta-D-xylose + Xylbeta(1-6)Glcbeta(1-4)Glc
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc + H2O
?
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc + H2O
?
-
-
-
?
chemically modified cellulose + H2O
oligosaccharide + ?
-
-
-
?
chemically modified cellulose + H2O
oligosaccharide + ?
-
best substrate
-
-
?
oligoxyloglucan + H2O
isoprimeverose + ?
the enzyme is involved in utilization of oligoxyloglucans as carbon sources
-
-
?
oligoxyloglucan + H2O
isoprimeverose + ?
the enzyme recognizes and releases isoprimeverose (alpha-D-xylopyranose-(1,6)-D-glucopyranose) units from the non-reducing ends of oligoxyloglucans. Xylopyranosyl-branching at the non-reducing ends was vital for IPase activity, and galactosylation at alpha-(1,6)-linked xylopyranosyl side chain completely abolishes activity. Hepta-oligoxyloglucan saccharide (Xyl3Glc4) substrate is preferred over tri-(Xyl1Glc2) and tetra-(Xyl2Glc2) oligoxyloglucan saccharides substrates. IpeA transfers isoprimeverose units to other saccharides, indicating transglycosylation activity
-
-
?
oligoxyloglucan + H2O
isoprimeverose + ?
the enzyme is involved in utilization of oligoxyloglucans as carbon sources
-
-
?
oligoxyloglucan + H2O
isoprimeverose + ?
the enzyme recognizes and releases isoprimeverose (alpha-D-xylopyranose-(1,6)-D-glucopyranose) units from the non-reducing ends of oligoxyloglucans. Xylopyranosyl-branching at the non-reducing ends was vital for IPase activity, and galactosylation at alpha-(1,6)-linked xylopyranosyl side chain completely abolishes activity. Hepta-oligoxyloglucan saccharide (Xyl3Glc4) substrate is preferred over tri-(Xyl1Glc2) and tetra-(Xyl2Glc2) oligoxyloglucan saccharides substrates. IpeA transfers isoprimeverose units to other saccharides, indicating transglycosylation activity
-
-
?
tamarind xyloglucan + H2O
oligosaccharide + ?
-
-
-
?
tamarind xyloglucan + H2O
oligosaccharide + ?
-
-
-
-
?
tamarind xyloglucan + H2O
oligosaccharide + ?
-
-
-
-
?
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
beta-D-xylose + (Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
-
-
-
-
?
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
beta-D-xylose + (Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
-
-
-
-
?
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + isoprimeverose
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
-
-
-
-
?
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + isoprimeverose
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
-
-
-
-
?
Xylbeta(1-6)Glcbeta(1-4)Glc + H2O
Xylbeta(1-6)Glc + beta-D-glucopyranose
-
-
-
-
?
Xylbeta(1-6)Glcbeta(1-4)Glc + H2O
Xylbeta(1-6)Glc + beta-D-glucopyranose
-
-
-
-
?
xyloglucan + H2O
isoprimeverose + ?
the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex
-
-
?
xyloglucan + H2O
isoprimeverose + ?
isoprimeverose-producing oligoxyloglucan hydrolase requires LacA for the removal of galactopyranosyl residues
-
-
?
xyloglucan + H2O
isoprimeverose + ?
the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex
-
-
?
xyloglucan + H2O
isoprimeverose + ?
isoprimeverose-producing oligoxyloglucan hydrolase requires LacA for the removal of galactopyranosyl residues
-
-
?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
-
-
-
-
?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
-
-
-
-
?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
-
highly specific for
isoprimeverose is split off from the nonreducing end of the backbone of the substrate
?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
Malus malus
-
-
-
-
?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
-
-
-
-
?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
-
-
-
-
?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
-
-
-
-
?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
-
specifically cleaves 1,4-beta-glucosyl-linkages
products are nona- and heptasaccharides
?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
-
-
-
-
?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
-
-
-
-
?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
-
-
-
-
?
additional information
?
-
-
main roles of enzyme are wall restructuring and integration/wall assembly
-
-
?
additional information
?
-
-
no substrate: xylan
-
-
?
additional information
?
-
-
no substrate: cellulose derivatives
-
-
?
additional information
?
-
-
no substrate: cellulose derivatives
-
-
?
additional information
?
-
-
no substrate: cellulose derivatives
-
-
?
additional information
?
-
-
no substrate: carboxymethylcellulose, swollen cellulose
-
-
?
additional information
?
-
-
no substrate: (1-3,1-4)-beta-glucan
-
-
?
additional information
?
-
-
no substrate: carboxymethylcellulose, swollen cellulose
-
-
?
additional information
?
-
-
enzyme allows for use of cellulose as carbon source by breaking down surrounding cellulose fibrils
-
-
?
additional information
?
-
-
no substrate: cellulose derivatives
-
-
?
additional information
?
-
-
enzyme is capable of hydrolizing xyloglucans within the native cell wall architecture, thereby increasing the cell wall extensibility
-
-
?
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oligoxyloglucan + H2O
isoprimeverose + ?
xyloglucan + H2O
isoprimeverose + ?
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
-
highly specific for
isoprimeverose is split off from the nonreducing end of the backbone of the substrate
?
additional information
?
-
oligoxyloglucan + H2O
isoprimeverose + ?
the enzyme is involved in utilization of oligoxyloglucans as carbon sources
-
-
?
oligoxyloglucan + H2O
isoprimeverose + ?
the enzyme is involved in utilization of oligoxyloglucans as carbon sources
-
-
?
xyloglucan + H2O
isoprimeverose + ?
the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex
-
-
?
xyloglucan + H2O
isoprimeverose + ?
the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex
-
-
?
additional information
?
-
-
main roles of enzyme are wall restructuring and integration/wall assembly
-
-
?
additional information
?
-
-
enzyme allows for use of cellulose as carbon source by breaking down surrounding cellulose fibrils
-
-
?
additional information
?
-
-
enzyme is capable of hydrolizing xyloglucans within the native cell wall architecture, thereby increasing the cell wall extensibility
-
-
?
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0.23
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 4.5, 60°C
1.32
substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 4.5, 60°C
19
-
pH 4.5, 40°C, substrate: tamarind xyloglucan
226
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc, pH 4.5, 60°C
296
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc, pH 4.5, 60°C
297
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc, pH 4.5, 60°C
422
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C
427
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glcol, pH 4.5, 60°C
85
-
with Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
additional information
-
overview: assay methods
1
-
pH 4.5, 40°C, substrate: chemically modified cellulose
1
-
pH 4.5, 40°C, substrate: beta-glucan
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Rose, J.K.C.; Braam, J.; Fry, S.C.; Nishitani, K.
The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: Current perspectives and a new unifying nomenclature
Plant Cell Physiol.
43
1421-1435
2002
Arabidopsis thaliana
brenda
Kato, Y.; Matsushita, J.; Kubodera, T.; Matsuda, K.
A novel enzyme producing isoprimeverose from oligoxyloglucans of Aspergillus oryzae
J. Biochem.
97
801-810
1985
Aspergillus oryzae
brenda
Hasper, A.A.; Dekkers, E.; Van Mil, M.; Van de Vondervoort, P.J.I.; De Graaff, L.H.
EglC, a new endoglucanase from Aspergillus niger with major activity towards xyloglucan
Appl. Environ. Microbiol.
68
1556-1560
2002
Aspergillus niger
brenda
Matsumoto, T.; Sakai, F.; Hayashi, T.
A xyloglucan-specific endo-1,4-beta-glucanase isolated from auxin-treated pea stems
Plant Physiol.
114
661-667
1997
Pisum sativum
brenda
Kaku, T.; Tabuchi, A.; Wakabayashi, K.; Kamisaka, S.; Hoson, T.
Action of xyloglucan hydrolase within the native cell wall architecture and its effect on cell wall extensibility in azuki bean epicotyls
Plant Cell Physiol.
43
21-26
2002
Vigna angularis
brenda
Irwin, D.C.; Cheng, M.; Xiang, B.; Rose, J.K.C.; Wilson, D.B.
Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca
Eur. J. Biochem.
270
3083-3091
2003
Thermobifida fusca
brenda
Vincken, J.P.; van den Broek, L.A.M.; van der Lei, D.D.; Beldman, G.; Voragen, A.G.J.
Fungal and plant xyloglucanases may act in concert during liquefaction of apples
J. Sci. Food Agric.
73
407-416
1997
Malus malus, Trichoderma viride
brenda
Tabuchi, A.; Kamisaka, S.; Hoson, T.
Purification of xyloglucan hydrolase/endotransferase from cell walls of azuki bean epicotyls
Plant Cell Physiol.
38
653-658
1997
Vigna angularis
-
brenda
Pauly, M.; Andersen, L.N.; Kauppinen, S.; Kofod, L.V.; York, W.S.; Albersheim, P.; Darvill, A.
A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme
Glycobiology
9
93-100
1999
Aspergillus aculeatus
brenda
Konishi, T.; Mitsuishi, Y.; Kato, Y.
Analysis of the oligosaccharide units of xyloglucans by digestion with isoprimeverose-producing oligoxyloglucan hydrolase followed by anion-exchange chromatography
Biosci. Biotechnol. Biochem.
62
2421-2424
1998
Penicillium sp., Penicillium sp. M451, Penicillium sp. M9
brenda
Yaoi, K.; Hiyoshi, A.; Mitsuishi, Y.
Screening, purification and characterization of a prokaryotic isoprimeverose-producing oligoxyloglucan hydrolase from Oerskovia sp. Y1
J. Appl. Glycosci.
54
91-94
2007
Oerskovia sp., Oerskovia sp. Y1
-
brenda
Matsuzawa, T.; Watanabe, M.; Kameda, T.; Kameyama, A.; Yaoi, K.
Cooperation between beta-galactosidase and an isoprimeverose-producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzae
FEBS J.
286
3182-3193
2019
Aspergillus oryzae (Q2U8V9), Aspergillus oryzae ATCC 42149 (Q2U8V9)
brenda
Matsuzawa, T.; Mitsuishi, Y.; Kameyama, A.; Yaoi, K.
Identification of the gene encoding isoprimeverose-producing oligoxyloglucan hydrolase in Aspergillus oryzae
J. Biol. Chem.
291
5080-5087
2016
Aspergillus oryzae (Q2U8V9), Aspergillus oryzae, Aspergillus oryzae ATCC 42149 (Q2U8V9)
brenda