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EC Tree
IUBMB Comments The enzyme is found in the fungus Geotrichum sp. M128. The substrate is a hemicellulose found in plant cell walls.
The enzyme appears in viruses and cellular organisms
Synonyms
spezyme cp, oxg-rcbh, cellulose 1,4-beta-cellobiosidase, beta-d-cellobiosidase, oligoxyloglucan reducing-end-specific cellobiohydrolase, oligoxyloglucan reducing end-specific cellobiohydrolase, beta-1,4-cellobiosidase,
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1,4-beta-D-glucan cellobiohydrolase
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1,4-beta-glucan cellobiohydrolase
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1,4-beta-glucan cellobiosidase
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beta-1,4-cellobiopyranosidase
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beta-1,4-cellobiosidase
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beta-1,4-glucan cellobiohydrolase
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beta-D-cellobiosidase
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cellobiohydrolase
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cellobiohydrolase I
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cellobiohydrolase II
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cellobiohydrolase, exo-
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cellobiosidase, 1,4-beta-glucan
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cellulose 1,4-beta-cellobiosidase
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exo-1,4-beta-D-cellobiohydrolase
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exo-beta-1,4-glucan cellobiohydrolase
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exocellobiohydrolase
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oligoxyloglucan reducing end-specific cellobiohydrolase
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oligoxyloglucan reducing-end-specific cellobiohydrolase
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OXG-RCBH
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alpha-D-xylopyranosyl-(1-6)-[alpha-D-xylopyranosyl-(1-6)-[alpha-D-xylopyranosyl-(1-6)-beta-D-glucopyranosyl-(1-4)]-beta-D-glucopyranosyl-(1-4)]-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranose + H2O = alpha-D-xylopyranosyl-(1-6)-[alpha-D-xylopyranosyl-(1-6)-beta-D-glucopyranosyl-(1-4)]-beta-D-glucopyranose + alpha-D-xylopyranosyl-(1-6)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranose
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hydrolysis of O-glycosyl bond
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oligoxyloglucan reducing-end cellobiohydrolase
The enzyme is found in the fungus Geotrichum sp. M128. The substrate is a hemicellulose found in plant cell walls.
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heptasaccharide XXXG
trisaccharide XG + tetrasaccharide XX
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xyloglucan heptasaccharide
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xyloglucan tetradecasaccharide
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enzyme substrate specificity to the tetradecasaccharide, XXXGXXXG, is analyzed. Wild-type OXG-RCBH generates XXXGXX and XG due to its strict specificity
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additional information
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oligoxyloglucan
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oligoxyloglucan
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oligoxyloglucan
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enzyme recognizes the reducing end of oligoxyloglucan and releases two glucosyl residue segments from the main chain
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oligoxyloglucan
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enzyme recognizes the reducing end of oligoxyloglucan and releases two glucosyl residue segments from the main chain
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oligoxyloglucan
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OXG-RCBH acts on xyloglucan oligosaccharides at the reducing end in exo mode
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additional information
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enzyme recognizes the reducing end of oligoxyloglucan and releases two glucosyl residue segments from the main chain
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additional information
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enzyme has endoglucanase activity, releases two glucosyl residue segments from the main chain
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additional information
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no activity with beta-1,3/4-glucan and carboxymethyl cellulose (CMC)
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oligoxyloglucan
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oligoxyloglucan
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15
with xyloglucan heptasaccharide as substrate
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sp. M128
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brenda
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Uniprot
brenda
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SwissProt
brenda
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brenda
M128
SwissProt
brenda
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CBHRE_GEOS1
Geotrichum sp. (strain M128)
812
0
87081
Swiss-Prot
Secretory Pathway (Reliability: 1 )
XGCA_EMENI
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)
810
0
87098
Swiss-Prot
Secretory Pathway (Reliability: 2 )
XGCA_NEOFI
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181)
815
0
87559
Swiss-Prot
Secretory Pathway (Reliability: 1 )
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by using the hanging-drop vapor-diffusion method. X-ray crystal structure of the OXG-RCBH-substrate complex is determined to a resolution of 2.4 A. OXG-RCBH consists of two seven bladed beta-propeller domains. There is a large cleft between the two domains, and a unique loop encloses one side of the active site cleft. Substrate bound to the cleft, and its reducing end is arranged near the loop region that is believed to impart OXG-RCBH with its activity
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hanging drop vapor diffusion method
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hanging- and sitting drop vapor diffusion method with polyethylene glycol 3000 and polyethylene glycol 400 as precipitants
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D465N
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inactive OXG-RCBH is co-crystallized with a heptaxyloglucan substrate (XXXG) under conditions similar to the wild-type. There are no differences in the overall structure between the mutant and the wild-type
DELTAG375-H385
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a deltaloop mutant (deletion mutant of the loop region, Gly375His385) is constructed. The mutant shows endo-activity with altered substrate recognition. More specifically, cleavage occurs randomly instead of at specific sites, most likely due to the misalignment of the substrate within the subsite. It is supposed that the loop imparts unique substrate specificity with exo-mode hydrolysis in OXG-RCBH
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4 - 8
stable at 45°C
646831
4 - 8
90% activity at 45°C
646831
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50
10% loss of activity after 10 min at pH 4
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90% activity after 10 min at pH 4
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purified by using a HiTrap DEAE FF column
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expressed in Escherichia coli
expression in Escherichia coli
overexpressed in Escherichia coli BL21(DE3) as a His-tagged fusion protein
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recombinant protein is expressed as insoluble inclusion bodies. Purified inclusion bodies were solubilized in 8 M urea. Renaturing by dialysis results in enzymatically active enzyme
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Yaoi, K.; Mitsuishi, Y.
Purification, characterization, cloning, and expression of a novel xyloglucan-specific glycosidase, oligoxyloglucan reducing end-specific cellobiohydrolase
J. Biol. Chem.
277
48276-48281
2002
Geotrichum sp. (Q8J0D2)
brenda
Yaoi, K.; Kondo, H.; Suzuki, M.; Noro, N.; Tsuda, S.; Mitsuishi, Y.
Crystallization and preliminary X-ray crystallographic study on a xyloglucan-specific exo-beta-glycosidase, oligoxyloglucan reducing-end specific cellobiohydrolase
Acta Crystallogr. Sect. D
59
1838-1839
2003
Geotrichum sp.
brenda
Yaoi, K.; Kondo, H.; Noro, N.; Suzuki, M.; Tsuda, S.; Mitsuishi, Y.
Functions and structures of xyloglucan hydrolases belonging to glycoside hydrolase family 74
J. Appl. Glycosci.
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169-176
2005
Geotrichum sp. (Q8J0D2)
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brenda
Yaoi, K.; Kondo, H.; Noro, N.; Suzuki, M.; Tsuda, S.; Mitsuishi, Y.
Tandem repeat of a seven-bladed beta-propeller domain in oligoxyloglucan reducing-end-specific cellobiohydrolase
Structure
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1209-1217
2004
Geotrichum sp. (Q8J0D2)
brenda
Yaoi, K.; Kondo, H.; Hiyoshi, A.; Noro, N.; Sugimoto, H.; Tsuda, S.; Mitsuishi, Y.; Miyazaki, K.
The structural basis for the exo-mode of action in GH74 oligoxyloglucan reducing end-specific cellobiohydrolase
J. Mol. Biol.
370
53-62
2007
Geotrichum
brenda
Yaoi, K.; Kondo, H.; Hiyoshi, A.; Noro, N.; Sugimoto, H.; Tsuda, S.; Miyazaki, K.
The crystal structure of a xyloglucan-specific endo-beta-1,4-glucanase from Geotrichum sp. M128 xyloglucanase reveals a key amino acid residue for substrate specificity
FEBS J.
276
5094-5100
2009
Geotrichum sp. M128 (Q8J0D2)
brenda
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