Information on EC 3.2.1.187 - (Ara-f)3-Hyp beta-L-arabinobiosidase

beta-L-arabinofuranosidase

GH 121 beta-L-arabinofuranosidase

GH121 beta-L-arabinobiosidase

hypBA2

xcv2729

XeHypBA2

4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O = 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose

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4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose hydrolase

The enzyme, which was identified in the bacterium Bifidobacterium longum JCM1217, is specific for (Ara-f)3-Hyp, a sugar chain found in hydroxyproline-rich glyoproteins such as extensin and lectin. The enzyme was not able to accept (Ara-f)2-Hyp or (Ara-f)4-Hyp as substrates. In the presence of 1-alkanols, the enzyme demonstrates transglycosylation activity, retaining the anomeric configuration of the arabinofuranose residue.

4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O

4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose

7 entries

arabinan + H2O

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carrot extension + H2O

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debranched arabinan + H2O

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potato lectin + H2O

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additional information

?

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6 entries

4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O

4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose

4 entries

Ca2+

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1 mM Ca2+ increases the enzymatic activity by 43.8%

EDTA

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1 mM EDTA decreases the enzymatic activity to 1.4%

0.0107

4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline

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in 50 mM sodium acetate (pH 6.0), at 37°C

2.7

4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline

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in 50 mM sodium acetate (pH 6.0), at 37°C

Xanthomonas euvesicatoria

4.5

assay at

5.5 - 6

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30

Bifidobacterium longum JCM 1217

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725455, 725546

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725455, 725546

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Xanthomonas euvesicatoria

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UniProt

Xanthomonas euvesicatoria 85-10

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UniProt

Xanthomonas euvesicatoria UPB139

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UniProt

additional information

evolution

malfunction

metabolism

physiological function

Bacteroides thetaiotaomicron

156797

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x * 156797, calculated from amino acid sequence

157000

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x * 157000, SDS-PAGE

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7 entries

additional information

4 entries

TALON metal affinity resin column chromatography

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expressed in Escherichia coli BL21(lambdaDE3) cells

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Xanthomonas euvesicatoria

gene XCV2729, belongs to operon xehypBA2-AA containing genes XCV2724, XCV2728, and XCV2729, DNA and amino acid sequence determination and analysis, and genetic structure. The promoter region of the operon (xehypBA2 and xehypAA) contains the plant-inducible promoter box for binding the regulator protein HrpX involved in pathogenicity, recombinant expression of wild-type and mutant enzymes without the signal peptide and fused with the trigger factor chaperone in Escherichia coli strain BL21(DE3), recombinant expression of the operon in transgenic Solanum lycopersicum strain Micro-Tom

the operon is upregulated by transcription factor HrpX